Biology Form 4 Paper III Question II Introduction

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Butterworth
04- 3313310
Bkt Mertajam
04- 537 5537
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Form 4 Paper III
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Question 2 Answering Techniques
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By: Clement Tan, Bsc.(Hons) Northwestern University
© Copyright reserved
04- 33 13310 ; 013- 533 9133
choonpintan@gmail.com
Prepared by Clement Tan
mqwertyuiopasdfghjklzxcvbnmqwert Copy right 2012

Butterworth Bkt Mertajam
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Paper Paper 1 (4551/1) Paper 2 (4551/2) Paper 3 (4551/3)

Aspect
Type of Objective Test Subjective Test Written Practical
Instrument
Type of questions Objective items: Subjective questions: Subjective questions:
• Multiple-choice (Practical type questions)
• Multiple- Part A Question 1: Structured
combination Structured questions item
– Each item consists of four Question 2: Open ended
opinions: A, B, C & D. Part B respond(Essay)
Restricted & Extended
respond essay questions
Number of 50 compulsory questions Part A Structured item:

questions 5 compulsory questions 2 Compulsory Questions.
-Question 1 requires the
Part B application of scientific
4 questions- Choose any skills
two(based on latest -Question 2 tests the
format since 2008) ability to plan and do an
experimental write-up.
Total marks 50 marks 100 marks 50 marks
Part A – 60 marks
Part B – 40 marks
Answering Blacken the chosen answer Write the answer in the Write the answer in the
Instructions on OMR sheet with 2B space provided in the space provided.
pencil only. question paper.
Duration 1 hour and 15 minutes 2 hours and 30 minutes 1 hour and 30 minutes
(75 minutes) (150 minutes) (90 minutes)
Requirement of Knowledge – 25 Knowledge – 10 Scientific Processing
Construct Understanding – 15 Understanding – 20 Skills – 16 aspects with
Application skills – 10 Application skills – 30 the maximum of 3 marks
Analyzing skills – 15 each + 2 marks.
Synthesizing skills – 15
Evaluating skills –10
Level of Difficulty: L M H • 40 easy scores • 10 easy aspects
Low: L: 50% Ratio 3 1 1 • 40 moderate • 30 moderate
Moderate: M: 30% scores aspects
• 30 easy items
High: H: 20%
• 10 moderate items • 20 difficult • 30 difficult
scores aspects
• 10 difficult items
Overall:
L:M:H = 5:3:2
Marking Dichotomous 1 or 0 mark Analytical marking Analytical
marking based
on rubric of 3
level.
Apparatus Scientific Calculator Scientific Calculator Scientific
Allowed Calculator
By: Clement Tan, Bsc.(Hons) Northwestern University 04- 33 13310 ; 013- 533 9133
© Copyright reserved choonpintan@gmail.com
04- 3313310 04- 537 5537
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General Info:
Question 2 of biology paper III requires the candidates to plan and write up a full experimental
write up.
Normally, you will be given a real life situation and you will need to apply what you learn in
scientific investigation to plan an experiment to solve the problem.
Example of Situation:
Situation 1:
House wife A uses warm water to wash her clothes using washing liquid which contains added
enzyme. The cleaning is more effective.
Situation 2:
Using the same washing liquid as in situation 1, housewife B uses cold water to wash her
clothes. The cleaning is less effective.
Based on both situations, design a laboratory experiment to study the effect of temperature on
the rate of enzyme reaction.
Experimental Write-Up (Fixed):
Then, after the situation given, SPM candidate has to do an experimental write up that consists of
the following parts:
Part Scientific Descriptions/Markings/Important Key Total Marks

Investigation Points
1. Problem a. A problem statement is also called a P1 = MV
Statement research statement. P2 = RV
b. A problem statement must always be 3m
in question form. (‘?’ has to be H = Question
there) P1 + P2 + H = 3m
c. A problem statement consists of three
criteria:
1. Manipulated
Variable(MV)
2. Responding
Variable(RV)
3. Relationship between
MV & RV.
Example: What is the effect of
temperature(MV) on the rate of enzymatic
reaction of salivary amylase(RV)?
2. Objective of a. State the objective/aim of the No mark. Just a tick
Investigation experiment given.
b. To study/investigate the effects of
MV on RV
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3. Hypothesis a. Hypothesis should be able to P1 = MV

answer your problem statement. 3m
b. Must have the following criteria: P2 = RV
1. MV H = Relationship
P1 + P2 + H = 3m
2. RV
MV = Temp…
3. Relate MV & RV. RV = Rate of enzyme
Format: When MV increases/decreases, reaction
RV increases/decreases. (no neutral H = increases/decreases
term accepted).
Example: When temperature(MV)
increases, the rate of enzyme reaction(RV)
increases.
4. Variables 1. Write down three variables: All three variable
a. MV correct
b. RV No mark just a tick
c. CV
2. CV must be one that will affect the
outcome of the experiment if it is
not kept constant. (time is
generally not accepted as CV)
5. List of 1. You need to list all the materials 3 marks max.
Apparatus & and apparatus needed to carry out
Materials the experiment. How many A & M
2. Materials: things that are used up needed depends on
or perishable in the experiment. the experiment.
3. Apparatus: is used to manipulate
variables to get results(could be
found in laboratory).
6. Techniques 1. Write down what is observed or Bonus mark = 1
Used measured(include the instruments mark.
used)
2. State the specific name of the
techniques used.
Example:
1. Using a stopwatch to record time taken
for the starch to disappear
2. Calculate the rate of enzyme reaction
using the formula 1/time
7. Experimental 1. Write the procedure clearly and All 5K = 3m

Procedures systematically which must include 3 to 4 K only = 2m
all of these steps: 2k only = 1m
K1: Setting up the 1K = 0m but tick
apparatus/materials (write 3)
K2: Handling the MV(write 1)
K3: Handling the RV(write 1)
K4: Handling the CV(write 1)
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K5: Precaution/ Accuracy of

experiment (write 1)
Example: Maintain water bath temp,
Rinse the apparatus, repeat expt to get
average reading
2. Written as a set of instructions, use
simple present tense, NOT in
passive voice.
8. Presentation 1. Use tables(graphs, charts can be Bonus # 2

of data used if requested in question). =1m
2. Construct the table with correct
titles & units.
3. MV in first column, RV in second
column, Calculation (if any) in third
column.
4. LEAVE the table blank (without
results or readings)
9. Conclusion 1. Rewrite your hypothesis correctly No mark, just a tick.

TOTAL MARKS 14 marks
Additional Comments (Where has

the remaining 3 marks gone?)
Question 2: Sample Answer Keys for SPM Biology Question #2

Construct Sample Answers Comments/remarks
1.Objective 1. To study the effects of temperature NO MARK ( just a tick)
on the rate of enzyme reaction
2. Problem Statement Able to related P1, P2 and H in a P1 = MV
(PS) question form P2 = RV 3m
1. What are the effects of H = Question
temperature on salivary P1 + P2 + H = 3m
amylase activity on starch? MV = Temperature/ cold
2. What is the effect of water, warm water
temperature on the rate of RV= Rate of enzyme reaction
enzyme reaction? (if student use a specific
3. What is the optimum enzyme then must be the
temperature for enzyme to work correct substrate)
effectively?
Reject : hot water

3. Hypothesis (Hp) Able to state the hypothesis relating 3 P1 = MV 3m
aspects (P1, P2 and H) P2 = RV
1. When the temp increases, the H = Relationship
rate of (amylase/ enzyme P1 + P2 + H = 3m
reaction (on starch) increase MV = Temp…
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(until the optimum temp) RV = Rate of enzyme

2. When the temp increases, the reaction
rate of enzyme reaction H = increases/decreases
increases.
4. Variables (Vr)
Manipulated : Temp of (medium of reaction)// warm
water and cold water
Responding : 1. The rate of enzyme reaction
(catalaysed by salivary
amylase)
2. The time taken for (the
completion of) the hydrolysis of
starch// time taken for effective
cleaning
Constants : 1. Volume of amylase/ All three variables correct
enzyme/washing No mark just a tick
2. Concentration of
amylase/enzyme/washing liquid
3. Volume of starch
(suspension)/suibstrate
4. Concentration of starch
(suspension)/substrate
5. pH value
6. Type on enzyme/washing liquid
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5. Apparatus Apparatus Materials 6A + 5M = 3m
and 1. Beaker 1. Starch 4/5 A + 3M = 2m
Materials 2. Test tube 2. Saliva/ 3 A + 2 M = 1m
(AM 3. Thermometer amylase/washing ½ A + 1M = 0m
4. Syringe/measuring liquid but tick
cylinder 3. Water
5. Stop watch 4. Ice cube
6. Bunsen burner + 5. Iodine solution/any
tripod stand/ wire other reagent for
gauze food test
6. Technique Able to state the operating responding variable B1 = 1m

(Tq) correctly,
3. Using a stopwatch to record time taken for the starch
to disappear/time taken for complete hydrolysis of
starch/time taken for the iodine (any food test) to
change colour。
4. Calculate the rate of enzyme reaction using the

formula 1/time
7. Procedure K1: Preparation of materials & apparatus(any 3 ) All 5K = 3m

(K) • Named substrate. eg: starch/clothe +stain/dirty cloth 3 to 4 K only = 2m
• Named enzyme/washing liquid 2k only = 1m
• Put/immerse test tubes into breaker/ water bath 1K = 0m but
• At same temp, mix/pour/stir tick
• Start stopwatch
• Named reagent for food test
• Repeat the food test every… minutes
• Diagram with at least 5 labels.
K2: Operating Fixed variable (any 1)
• Volume/concentration of substrate…ecf from app
and material
• Volume/concentration of enzyme… ecf from app
and material
K3 : Operating Responding variable (any 1)
• Record the time taken for the hydrolysis of starch
(any stain/substrate given)/ change of colour of
indicator in food test
• Record the result/time taken in a table
K4: Operating manipulated variable (any1)
K5 : Precaution/ Accuracy of experiment(any1)
• Maintain water bath temp
• Rinse the dropper/mouth (before taking saliva)
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• Repeat experiment to get average reading

8. Recording Able to construct a table of results B2 = 1m
data/ Result Temperature ºC Time taken for Rate of
(RD) the starch to reaction tick
disappear/ min /min -¹
9.Conclusion A hypothesis statement No mark, just a tick

(CN)
Planning 7-9 ticks = 3m
Experiment 4-6 ticks = 2m
03 2-3 ticks = 1m
Sample Answer for Procedure

1. Use a syringe to put 5ml of 1% starch (suspension) into each of the test tubes, labeled
A1,B1,C1,D1 and E1.
2. Use a second syringe to put 2ml of saliva/amylase solution into each of the test tubes,
labeled A2,B2,C2,D2 and E2
3. Immerse/put test tubes A1 and A2, (B1 and B2, C1 and C2, D1 and D2, E1 and E2) into
water bath with temp at 10ºC/cold water.
4. Mix/pour/stir the enzyme with starch from test tube into A1 into test tube A2(must be from
the same temp before mixing)
5. Start the stop watch (immediately)
6. Use a dropper to put a drop of mixture from test tube A2 onto the iodine solution in the first
groove of the white tile.
7. Repeat the iodine test for every minute for 10 minutes.
8. Rinse the dropper after each sampling.
9. Record the time taken for the complete hydrolysis of starch/ change of iodine colour…
10. Keep/maintain the water bath at their respective temp.
11. Repeat the experiment with temp 30ºC/warm water.
12. Record the time taken/ result in a table.
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Sample Question 1：
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Sample Question 2：
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Sample Question 3:
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Sample Question 4:
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Question 5：
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Question 6:

Biology Form 4 Paper III Question II Introduction

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vbnmqwertyuiopasdfghjklzxcvbnmq

tyuiopasdfghjklzxcvbnmqwertyuiopa Clement Tan

mqwertyuiopasdfghjklzxcvbnmqwert Copy right 2012

Paper Paper 1 (4551/1) Paper 2 (4551/2) Paper 3 (4551/3)

Number of 50 compulsory questions Part A Structured item:

Experimental Write-Up (Fixed):

Part Scientific Descriptions/Markings/Important Key Total Marks

3. Hypothesis a. Hypothesis should be able to P1 = MV

7. Experimental 1. Write the procedure clearly and All 5K = 3m

K5: Precaution/ Accuracy of

8. Presentation 1. Use tables(graphs, charts can be Bonus # 2

9. Conclusion 1. Rewrite your hypothesis correctly No mark, just a tick.

Additional Comments (Where has

Question 2: Sample Answer Keys for SPM Biology Question #2

Reject : hot water

(until the optimum temp) RV = Rate of enzyme

6. Technique Able to state the operating responding variable B1 = 1m

4. Calculate the rate of enzyme reaction using the

7. Procedure K1: Preparation of materials & apparatus(any 3 ) All 5K = 3m

• Repeat experiment to get average reading

9.Conclusion A hypothesis statement No mark, just a tick

Sample Answer for Procedure

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