Protein Quantification

In order to assess the biological function of proteins and their modifications for
understanding signaling mechanisms within cells as well as specific
biomarkers to disease, it is important to study the change of proteome under
different experimental conditions. The emerging technology of mass
spectrometry-based quantitative proteomics provides a powerful tool to
quantitatively and systematically assess quantitative differences in protein
profiles of distinct samples and is increasingly becoming a significant
component of biomedical and clinical research.

Protein Quantification Service

Discovery-based quantitative proteomics compares the proteome of a

diseased sample versus normal at a global scale, and has been widely used to
study various human diseases with the goal to identify biomarkers and/or
reveal the pathogenesis of diseases. Methods for acquiring quantitative
proteomics data are continually developing with very accurate stable isotope
labeling (SIL) and label-free approaches.

SIL provides chemically equivalent but isotopically different interior standards

for each peptide/protein for direct comparison of mass spectral signal
intensities that represent relative abundance Common SIL strategies include
protein and peptide level labeling strategies such as:

SILAC: stable isotope labeling of amino acids in cell culture,a global method
whereby all translated proteins have isotope labels metabolically incorporated
at selected amino acid residues;
ICAT: isotope-coded affinity tags, a technique that labels cysteine residues at
the protein level
iTRAQ: multiplexed isobaric tags for relative and absolute quantification;
GIST: global internal standard technology, a global post-digestion labeling
method that labels primary amine groups (peptide N-terminus and lysine
residues)
ISIL: an extension of GIST called in-gel stable isotope labeling, a method that
labels primary amine groups of proteins (protein N-terminus and lysine
residues) directly from gel separated samples.
Label-free: approaches to quantitative proteomics have gained prominence in
recent years since no additional chemistry or sample preparation steps are
required.
Mass spectrometry-based approaches for targeted protein quantification are
based on the concept of isotope dilution mass spectrometry techniques
commonly used for the detection of small molecules. The approach is
comparable to modern advanced antibody-based methods, such as the
multianalyte profiling platform (MAP), offering multiplexing detection capability
with an excellent dynamic range.

With years’ experience in advanced experiment equipment, Creative

Proteomics can provide a variety of proteomics services to assist your
scientific research, including:

iTRAQ-based proteomics analysis service

TMT-based proteomics analysis service
SILAC-based proteomics analysis service
Absolute quantification (AQUA) service
Label-free quantification service
Semi-quantitative proteomics analysis service
Parallel Reaction Monitoring (PRM)
SRM&MRM
As one of the leading omics industry company, Creative Proteomics now is
opening to provide a series of protein quantification services for our customers.
Our service guarantees accurate and reliable results, at quick turnaround time.
Please feel free to contact us and see how we can help to address your
problems.