FACULTY OF ENGINEERING AND TECHNOLOGY

DEPARTMENT OF CHEMICAL ENGINEERING

COURSE PLAN
Course Code: CH1106 Course Title : Introduction to Biochemical Principles
Semester : IV (B.TECH) Course Time: Jan – May
Required Text Books:
1. Peter. F, .Stanbury, Alan Whitaker, “Principles of Fermentation Technology” 2ndEditon,
Buterworth – Heineman (an imprint of Elsevier), 195.
2. D.G. Rao, Introduction to Biochemical Engineering Tata McGraw‐Hill Education, 2005 
3. Shruler and Kargi.,Bioprocess Engineering, Basic concenpts, 2nd edition
4. Pauline. M,.Doran. “Bioproces Enginering Principles”;Academic pres. 195.
Objectives:
To familiarize
1. To study the chronological development of bio process technology design and construction of
fermentor and parameters to be monitored and controlled in biochemical process.
2. To study the various media for fermentation process
3. To teach the principle and kinetics of sterilization methods
4. To study the stoichiometry and energetics of cell growth and product formation
5. To evaluate the kinetics and mechanism of microbial growth

Assessment Details:
Cycle test I : 10 marks Cycle Test II : 10 marks
Surprise Test /Assignment : 5 marks Model exam : 20 marks
Attendance : 5 Marks

Test Schedule:

S.No Test Topics Duration

1 Cycle test-I Unit I & II 1.5 hrs
2 Cycle test-II Unit III,VI 1.5 hrs
3 Model Exam Unit I to V 3 hrs

Outcomes
Students who have successfully completed this course will have full understanding of the following
concepts
Course Outcome Program Outcome
This course helps the students Students will:
• To understand the scope, development,
importance, applications of Biochemical • Be able to understand the role of
engineering/ engineer biochemical engineer in industries.
• To understand the various fermentation • Be able to develop a typical
processes fermentation unit/ processes for
• To understand the concepts of production and recovery of bio-
Continuous fermentation, aeration, products.
agitation and scale up of fermentation
unit
• To understand the operation,
measurement and control system of
fermentation unit, product recovery
system, and enzyme engineering

1
Detailed Session plan:

Introduction: Chronological development of bioprocess technologies, basic configuration

of fermentor and accessories, outline of an integrated bioprocess and the various unit
operations involved in bioprocesses, Monitored and controlled of various parameter in
fermentation process.
Sessi Topics to be covered Time Ref Teaching Testing method
on (min) method
No.
1 Definition & scope of 50 1-2 BB Group Discussion
Biochemical processes
2 Chronological development 50 1- 2 BB Group Discussion /
of bioprocess technologies Assignment
3 Comparison of Chemical 50 1-2 BB Group Discussion
and Biochemical Processes
4 Outline of an integrated 50 1-2 BB GD / Assignment
bioprocess
5 The various unit operations 50 1-2 BB Group Discussion
involved in bioprocesses
6 Physio chemical parameter 50 1-2 BB Group Discussion /
Monitoring in fermentation Assignment
process.
7 Biological parameter 1-2 BB Group Discussion /
Monitoring in fermentation Assignment
process
8 Controlled of various 1-2 BB Group Discussion /
parameter in fermentation Assignment
process
9 various types of control 1-2 BB Group Discussion /
system in fermentation Assignment
process
Raw materials and media design: Selection of good medium, medium requirements for
fermentation processes, carbon, nitrogen, minerals, vitamins and other complex nutrients,
oxygen requirements. Designing of media for fermentation process, Types of media-
simple, complex and crude media, design and usage of various commercial media for
industrial fermentations.
10 Selection of good medium 50 1-3 BB Group Discussion
11 Medium requirements for 50 1-3 BB Group Discussion
fermentation processes
12 Selection of carbon source- 50 1-3 BB Group Discussion
synthetic & crude
13 Selection of nitrogen 50 1-3 BB Group Discussion
source-synthetic & crude
14 Micro nutrients- 50 1-3 BB Group Discussion /
Minerals,Vitamins, etc Assignment
15 Designing of media for 50 1-3 BB Group Discussion
fermentation process
16 Different types of media 50 1-3 BB GD / Class test

2
17 Simple, Complex and 50 1-3 BB Group Discussion
Crude media
18 Usage of various 50 1-3 BB Group Discussion/
commercial media for Class test
industrial fermentations.
Sterilization: Types- Thermal death kinetics of micro organisms, batch and continuous
heat sterilization of liquid media, filter sterilization of liquid media and air.
Microorganism: Isolation, preservation and improvement of industrialy important micro-
organisms, development of inoculums for industrial fermentations. Different types of
fermentations process and its applications.

19 Introduction to Sterilization 50 1-3 BB Group discussion

20 Types of sterilization methods 50 1-3 BB Group discussion
22 Thermal death kinetics of 50 1-3 BB Group Discussion
micro organisms
23 Batch and continuous heat 50 1-3 BB Group Discussion /
sterilization of liquid media Assignment
24 Filter sterilization of liquid 50 1-3 BB Group Discussion
media and air
25 Isolation, preservation of 50 1-3 BB Assignment / Class
microorganism test
26 Development of inoculums 50 1-3 BB Group Discussion
for industrial fermentations
27 Different types of 50 1-3 BB Group Discussion /
fermentations process and Seminar
its applications.
Stoichiometry: Cell growth and product formation, elemental balances, degress of
reduction of substrate and biomass available, electron balances, yield coefficient of
biomass and product formation, maintenance co-efficient, energetic analysis of microbial
growth and product formation, oxygen consumption and heat evolution in aerobic
cultures, thermodynamic efficiency of growth.
.
28 Cell growth Kinetics 50 1-4 BB Group Discussion
29 Product formation 50 1-4 Group Discussion /
Kinetics Seminar
30 Elemental & Electron 50 1-4 BB GD
balances for
Biochemical process
31 Degress of reduction 50 1-4 BB GD / Class test
for substrate and
biomass
32 Yield & maintenance 50 1-4 BB GD
coefficient of
biomass and product
33 Energetic analysis of 50 1-4 BB GD / Assignment
microbial growth and
product formation
34 Oxygen consumption 50 1-4 BB Group Discussion

3
 aerobic cultures
35 Thermodynamic 50 1-4 BB Group Discussion /
efficiency of growth. Seminar

36 Heat evolution in 50 1-4 BB Group Discussion /

aerobic cultures Seminar
Kinetics: Phases of cell growth in batch cultures, simple unstructured kinetic models for
microbial growth, Monod model, growth of filamentous organisms. Growth associated
(primary) and non-growth associated (secondary) product formation kinetics, Leudking –
Piret models, substrate and product inhibition on cell growth and product formation.

37 Cell growth in batch 50 2-4 BB Group Discussion

cultures
38 Simple unstructured 50 2-4 BB GD
kinetic models for
microbial growth
39 Monod model for 50 2-4 BB Group Discussion
fermentation process
40 Growth associated 50 2-4 BB GD
product formation
Kinetic
41 Non-growth 50 2-4 BB Group Discussion /
associated product Seminar
formation kinetics
42 Leudking – Piret 50 2-4 BB Group Discussion /
models Seminar
43 Substrate and product 50 2-4 BB Group Discussion /
inhibition on cell Seminar
growth
44 product formation 50 2-4 BB Group Discussion /
Kinetic Seminar
45 Revision of all units 50 1-4 BB Group Discussion