Experiment No.

Iodometry

Iodine Value of Canola Oil

I. DATA AND RESULTS

A. Weighing Measurement

Sample: Canola Oil

Weight of sample 1: 0.1298 g

Weight of sample 2: 0.1380 g

B. Titration Data

Volume Reading Trial 1 Trial 2 Blank

Initial Reading 0.00 mL 0.00 mL 0.00 mL

Final Reading 36.00 mL 45.35 mL 12.30 mL (>50 mL)

Total Volume Used 36.00 mL 45.35 mL 62.30 mL

Volumetric Solution Used: 0.1N Sodium Thiosulfate

NF: 0.995588

Prepared by: Navarro, Thea

II. POST- LABORATORY COMPUTATIONS

[126.90 [VB − VS ]N ]
Iodine Value =
10w
[126.9 (62.30mL − 36.00mL)(0.1 x 0.995588)]
Iodine ValueTrial 1 = = 255.9896057
10 x 0.1298 g

[126.9 (62.30mL − 45.35mL)(0.1 x 0.995588)]

Iodine ValueTrial 2 = = 155.1786222
10 x 0.1380 g
 |Iodine ValueTrial 1 − Iodine ValueTrial 2 |
%Deviation = x 100
|Iodine Valueaverage |

|255.9896057 − 155.1786222|
%Deviation = x 100 = 49.0364%
|205.584114|

III. DISCUSSION

In the assay of obtaining Iodine value for canola oil, the solution is left to stand

for 30 minnutes, after addition of iodochloride, to allow complete liberation of

iodine. (Fisher & Hart, 1971, p. 74). The procedure requires addition of

iodobromide TS, however, deviation to iodochloride was done, since the former

was not available in the laboratory. The endpoint was observed with the discharge

of the blue color. In this assay, the blank consumed more than 50 mL. The

disappearance of the blue color was observed after 62.30 mL of the titrant was

consumed. However, the blank was overly titrated, so accuracy in the calculations

is reduced. Trial 1 consumed 36.00 mL of the titrant with the persistence of a lightly

colored solution, indicating endpoint. Trial 2 on the other hand, consumed 45.35

mL of the titrant, upon determination of endpoint. The calculated deviation between

the two trials is 49.0364%. The very high deviation of the trials can be due to the

incomplete reaction of the solution that should have been completed upon standing

of the solution. Another factor could be degradation iodine due to light exposure.

Both iodine values obtained in the assay do not comply to the official requirement

as indicated by the USP.

IV. CONCLUSION AND RECOMMENDATION

The calculated amount of Iodine Value for both trials are more than the

official requirement, which is from 110-126 (USP35-NF30, 2012, p.1726), and

the calculated deviation between trials are too high. Therefore, the

computation shows that the calculated values are inaccurate and imprecise.

V. REFERENCES

United States Pharmacopeial Convention Inc. (2012). United States

Pharmacopeia 35st Revision and National Formulary 30th Edition.

Maryland: USPC, p. 1726

Hart, F., & Fisher, H. (1971). Modern Food Analysis. New York: Springer

Verlag, p.174