1.

NTG
TRYPTONE YEAST EXTRACT AGAR

Suspend 24 g of powder in 1 L of distilled water and bring to the boil. Distribute into
containers and sterilize in the autoclave at 121ºC for 15 minutes.

2.COLIFORMI SI E. COLI
CHROMOGENIC COLIFORMS AGAR ISO 9308-1 (CCA Agar, ISO)
Suspend 29,45 g of powder in 1 L of distilled water and bring to the boil until fully
dissolved. Do not autoclave nor overheat. Mix well and distribute into Petri dishes
avoiding bubble formation. The finished plates remain effectives for at least one month if
stored in the dark between 2-8ºC.

3.ENTEROCOCI
SLANETZ BARTLEY AGAR BASE

Suspend 43,4 g of powder in 1 L of distilled water and bring to the boil. Sterilize in the
autoclave at 121ºC for 15 minutes. Cool to 50°C and add 10 mL/L of 1% TTC sterile
solution (Art. No. 06-023). Mix well and distribute into sterile Petri dishes immediately.

BILE ESCULIN AZIDE AGAR

Suspend 56.6 g of powder in 1 L of distilled water and bring to the boil. Distribute in
suitable containers and sterilize in the autoclave at 121ºC for 15 minutes. Cool to 50-
60ºC and pour plates to 3-5 mm thickness. These plates can be stored at 2 -8ºC for up to
two weeks.

4.CLOSTRIDIUM
Tryptose Sulfite Cycloserine Agar (TSC Agar)

Suspend 45 g of powder in 1L of distilled water and let soak minutes. Bring to a boil and
distribute volumes of 250 ml or 100 ml in suitable containers. Sterilize the autoclave for
10 minutes at 121 ° C. Cool to 60 ° C and add 1 vial of Selective Supplement D-
Cycloserine (Ref. 06-116LYO1 or 06-743LYO1) to each portion of media. Mix well and
distribute on plates. If you wish yolk, while adding the antibiotic, sterile egg yolk (Ref.
06-016) at 80 ml / l.