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ABSTRACT
The concepts of diffusion and osmosis have been identified as very central towards the
understanding of many life processes and are also integrated into other science subjects such as
physics, and chemistry. To assess students’ conceptual understanding and application of
diffusion and osmosis, this study developed and validated a 25- item diagnostic test on diffusion
and osmosis (DTDO), a two-tier test consisting of twelve original items obtained from Diffusion
Osmosis Diagnostic Test (DODT) as well as thirteen newly developed and validated items that
aligned perfectly well with the Senior Secondary School curriculum in Nigeria and also the
university curriculum for Diploma students. The diagnostic instrument was administered to 806
Diploma students (476 biology majors and 330 non- biology majors) who had received one year
instruction in biology, chemistry and physics and had just written both the final year diploma
examinations and the Joint Universities Preliminary Examinations Board (JUPEB) examinations
in a large federal university in Nigeria and were seeking direct entry admission into Nigerian
universities or partnering foreign tertiary universities. The content of DTDO covered all aspects
of diffusion and osmosis in all life processes. The process of development of the items
encompassed content, expert and incremental validity as well as obtaining difficulty and
discriminating indices for the matched pairs of items. Cronbach alpha was used to estimate the
internal consistency of the items in addition to using standard error of measurement for obtaining
bandwidth of scores in a single test administration. The study revealed incremental validity of
DTDO over DODT. Also, lack of conceptual understanding and application was evident in
students’ responses to items in DTDO. The findings …..
INTRODUCTION
Reforms in mastery learning including Science Technology and Society (STS) initiatives
emphasise the teaching and learning of science in the context of human experiences. However,
only few researchers have evaluated the effectiveness of teaching programmes in the classroom
and the process of arriving at an answer rather than simply requiring students to regurgitate the
“right” answer – whether or not they understand either the answer or its justification. (Lawson,
1988; Glordan, 1996, Gallagher, 2000).
With the constructivism movement in science education, a large body of empirical data have
been provided that shows that students often come to science classes with conceptions
concerning a whole range of scientific domains that do not coincide with accepted scientific
thought (Carmichael et al. 1990; Duschl and Gitomer, 1991; Driver et al. 1994, Dfundt and Duit,
1940, Tekkaya, 2003, Chi, 2005,Tsui and Treagust, 2009).
The four major Worldwide Reforms in Science Education before the turn of the 21st Century
embrace four major goals which are: Science for all will lead to better development (Jenkins,
1997), Teaching for understanding and application of science knowledge and principles will lead
to productive work (Scott, 1998), Inclusion of a broader view of science in the curriculum will
incorporate local practices (Cobem, 1998), Less is better (this will avoid overloading the
curriculum) (Solomon, 1999). These major goals aim at attaining mastery in learning. However,
by the turn of the century, Gallagher (2000) affirmed that only a small percentage of students
who studied science in high school came out with any degree of understanding or “love” of
science. Eyibe (1990), Jegede (1992), Okebukola (1997) and Salau (1995) all asserted that a
large number of students find science learning difficult, boring and not interesting. Hence,
different authors and researchers have investigated the causes for the low learning outcomes.
Evidence from literature indicate that assessment also need to be broadened (Baker & Stites,
1991: Cizek & Rochor, 1994, Ferrara & Mctighe, 1992: Stiggins, 1991a, Busari, 1997; 2001,
Udeani, 2002; Baiyelo, 2000). Gallagher (2000) advocated strongly that there should be a shift
from the old vision about teaching and learning science to the current reform vision in science
teaching. In simple terms, the old vision about teaching and learning has the following
attributes:-
The 21st century reforms in science teaching have the following characteristics:
Knowledge is not a commodity that can be transmitted but the result of individual,
personal transformation of factual elements and relationships into a coherent form by the
learner. In concise terms, this vision holds that knowledge must be constructed by
individual learners.
Teaching is viewed as guiding the students towards valid construction of knowledge,
recognizing that unguided construction of knowledge frequently does not conform to the
scientific canon.
Learning is a process of making sense of new information and reconciling new and prior
knowledge to create a new level of understanding and application.
Assessment is viewed as formative in guiding both teacher and students toward deeper
understanding of and reasoning about the instructional topic. (Gallagher, 2000)
According to Gallagher (2000), if teachers are to participate in the contemporary reform
in science teaching, it is essential that they adopt this new vision about teaching and
learning and abandon the former one.
More than two decades ago, Odom and Barrow (1995) developed and applied a two-tier
diagnostic test named the diffusion and osmosis diagnostic test (DODT) on college
biology students to assess their understanding of diffusion and osmosis after a course of
instruction. Their results revealed that the performance of the college biology majors was
consistently poor, and scores obtained by college non- biology majors and high school
students were even lower. Twelve years later after the first report, Odom and Barrow
(2007) investigated responses and the level of certainty among 58 high school students
who responded to the DODT after a week of instruction on diffusion and osmosis.
Responses among the subjects were conspicuously similar to those obtained previously.
Furthermore, the confidence level of the subjects was assessed and discovered that the
students displayed high level of confidence on their incorrect responses.
In a more recent research by Fisher, Williams, and Lineback (2011), a two- tier
diagnostic tool containing 18 items on Diffusion and osmosis named Osmosis and
diffusion conceptual assessment(ODCA), some adopted or modified from the previous
work of Odom and Barrow (DODT) was administered among students at a large public
university. Responses from ODCA were remarkably similarly to the responses to DODT
collected from students 15 years earlier.
This paper assesses students’ conceptual understanding and application of diffusion and
osmosis, by using an enhanced 25- item diagnostic test on diffusion and osmosis (DTDO), a two-
tier test consisting of twelve original items obtained from Diffusion Osmosis Diagnostic Test
(DODT) as well as thirteen newly developed and validated items that aligned perfectly well with
the Senior Secondary School curriculum in Nigeria and also the university curriculum for
Diploma students. The diagnostic instrument was administered to 806 Diploma students (476
biology majors and 330 non- biology majors) who had received one year instruction in biology,
chemistry and physics and had just written both the final year diploma examinations and the
Joint Universities Preliminary Examinations Board (JUPEB) examinations in a large federal
university in Nigeria and were seeking direct entry admission into Nigerian universities or
partnering foreign tertiary universities. The content of DTDO covered all aspects of diffusion
and osmosis in all life processes. The process of development of the items encompassed content,
expert and incremental validity as well as well as obtaining difficulty and discriminating indices
for the matched pairs of items.
STATEMENT OF THE PROBLEM
There is considerable research evidence in science education in the past decades to support the
notion that students construct their own explanations for scientific phenomena, and these
explanations may be different from accepted scientific explanations. Also, there is a dearth in
conceptual assessment instruments in biology (D’Avanzo, 2008,Chi and Roscoe,2002) hence the
need to develop a 2-tier Diagnostic Test in Diffusion and Osmosis (DTDO) to assess student
conceptual understanding and application of Diffusion and Osmosis.
Similarly, diffusion and osmosis are fundamental concepts which could enhance the
understanding of many important life processes. Diffusion is the primary method of short
distance transport in cells and cellular systems. Similarly, understanding of osmosis concepts is
vital to understanding of water intake by plants, water balance in land and aquatic creatures,
turgor pressure in plants, and transport in living organisms. Also, diffusion and osmosis are
closely related to key concepts in physics and chemistry such as permeability, solutions, and the
particulate nature of matter (Friedler, Amir, & Tamir, 1987).
The choice of course content on Osmosis and Diffusion as topics for this study was informed by
the reports released from WAEC Chief Examiners’ Reports (2000, 2003, 2005, 2006, 2012) on
the poor performance of students in cell and environment/transport in animal and plant aspects of
biology. Similarly, an examination of the science scheme of work of the Diploma programme
showed that the students had concluded topics on diffusion and osmosis and related concepts and
had written their last paper.
THEORETICAL FRAMEWORK
The constructivist theory of learning assumes that learning is seen as an adaptive process, one in
which learners’ conceptual schemes are progressively reconstructed so that they are in keeping
with a continually wider range of experiences and ideas. It is also seen as an active process of
sense making over which the learner – has some control.
According to Yager (1990), every individual construct and reconstructs knowledge in the process
of assimilating knowledge and relating it to real life. It is favoured for the following:
RESEARCH QUESTIONS
Research questions
1. What is the level of students’ conceptual understanding and application of diffusion and
osmosis?
2. Is there any difference in the conceptual understanding and application of each of diffusion
and osmosis among biology major and non-biology major among diploma students?
3. Are there differences in the level of conceptual understanding and application of diffusion and
osmosis of biology major and non-biology major among diploma students?
4. Does gender have any effect on students’ conceptual understanding and application of
diffusion and osmosis among diploma students?
5. In which specific areas of diffusion and osmosis do biology major and non-biology major
among diploma students show the most misconceptions?
Research hypothesis
METHODOLOGY
This study developed and validated a 25- item diagnostic test on diffusion and osmosis (DTDO),
a two-tier test consisting of twelve original items obtained from Diffusion Osmosis Diagnostic
Test (DODT) as earlier developed by Odom and Barrow (1995) well as thirteen newly developed
and validated items that aligned perfectly well with the Senior Secondary School curriculum in
Nigeria and also the university curriculum for Diploma students. The newly developed 13- items
diagnostic test on Diffusion and Osmosis carefully selected from past WAEC questions and
restructured to fit into the 2- tier diagnostic test was administered to 40 participants made up of
SSS students and Biology teachers. The difficult to understand questions were further
restructured as based on teachers and students responses to items and interview. The items
covered the existing content areas of the particulate and random nature of matter, concentration
and tonicity, process of diffusion and process of osmosis originally itemized by Odom and
Barrow. Also, the 25 items were further screened by two senior lecturers in cell biology and
genetics department of a big federal university for suitability of the content and items for
Diploma students. Comments from these experts further helped to validate the instrument. The
25- items diagnostic test was then administered to 20 diploma students. The reliability coefficient
of 0.86 was obtained.
The process of development of the items encompassed content, expert and incremental validity
as well as obtaining difficulty and discriminating indices for the matched pairs of items.
Cronbach alpha was used to estimate the internal consistency of the items in addition to using
standard error of measurement for obtaining bandwidth of scores in a single test administration.
The diagnostic instrument was administered by the researcher and three research assistants to
806 Diploma students (476 biology majors and 330 non- biology majors) who had received one
year instruction in biology, chemistry and physics and had just written both the final year
diploma examinations and the Joint Universities Preliminary Examinations Board (JUPEB)
examinations in a large federal university in Nigeria and were seeking direct entry admission into
Nigerian universities or partnering foreign tertiary universities. These students were ready to
write their last JUPEB practical paper and were all gathered in a big hall waiting for the exam
when the 2- tier diagnostic test was conducted.
The content of DTDO covered all aspects of diffusion and osmosis in all life processes. The
DTDO was SAMPLE AND SAMPLING TECHNIQUE
DATA COLLECTION
DATA ANALYSIS
Table 1a. Percentage of Male Biology non-majors selecting correct choice, reason and
combination of choice and reason for the25 items on the Diffusion and Osmosis Test
Table 1b. Percentage of Female Biology non-majors selecting correct choice, reason and
combination of choice and reason for the25 items on the Diffusion and Osmosis Test
Female (N2=136)
1 73.52941 74.26471 61.02941
2 41.91176 27.20588 11.02941
3 55.14706 30.88235 24.26471
4 5.147059 43.38235 2.205882
5 27.20588 36.02941 19.85294
6 37.5 33.08824 21.32353
7 40.44118 58.82353 31.61765
8 33.08824 19.85294 8.823529
9 33.82353 16.91176 9.558824
10 43.38235 30.14706 17.64706
11 17.64706 28.67647 8.823529
12 54.41176 39.70588 21.32353
13 31.61765 35.29412 13.23529
14 46.32353 47.79412 35.29412
15 52.94118 49.26471 36.02941
16 32.35294 36.02941 23.52941
17 49.26471 34.55882 21.32353
18 61.76471 49.26471 43.38235
19 54.41176 56.61765 41.91176
20 49.26471 53.67647 38.97059
21 39.70588 33.82353 19.85294
22 57.35294 47.79412 41.17647
23 29.41176 34.55882 15.44118
24 49.26471 46.32353 38.97059
25 50.73529 50 33.08824
Table 1c. Percentage of Biology non-majors selecting correct choice, reason and
combination of choice and reason for the25 items on the Diffusion and Osmosis Test
Table 2b. Percentage of female Biology majors selecting correct choice, reason and
combination of choice and reason for the25 items on the Diffusion and Osmosis Test
Female (N2=280)
2. The particulate nature and random 2, 3, 6, 16, 18, 47.77 38.40 26.03
motion of matter 22
Table 1b. Percentage of Female Biology non-majors selecting correct choice, reason and
combination of choice and reason for the25 items on the Diffusion and Osmosis Test
Female (N2=136)
2. The particulate nature and random 2, 3, 6, 16, 18, 47.67 37.38 27.45
motion of matter 22
Table 1c. Percentage of Biology non-majors selecting correct choice, reason and
combination of choice and reason for the25 items on the Diffusion and Osmosis Test
2. The particulate nature and random 2, 3, 6, 16, 18, 47.73 37.98 26.62
motion of matter 22
Table 2b. Percentage of female Biology majors selecting correct choice, reason and
combination of choice and reason for the25 items on the Diffusion and Osmosis Test
Female (N2=280)
2. The particulate nature and random 2, 3, 6, 16, 18, 55.24 41.79 32.86
motion of matter 22
Table 2c. Percentage of Biology majors selecting correct choice, reason and combination of
choice and reason for the25 items on the Diffusion and Osmosis Test
2. The particulate nature and random 2, 3, 6, 16, 18, 55.11 42.09 33.33
motion of matter 22
RESULTS
CONCLUSION
RECOMMENDATIONS
APPENDIX
REFERENCES
Table 1a. Percentage of Male Biology non-majors selecting correct choice, reason and
combination of choice and reason for the25 items on the Diffusion and Osmosis Test
Table 2a. Percentage of male Biology majors selecting correct choice, reason and
combination of choice and reason for the25 items on the Diffusion and Osmosis Test
Table 2b. Percentage of female Biology majors selecting correct choice, reason and
combination of choice and reason for the25 items on the Diffusion and Osmosis Test
Female (N2=280)
Table 2c. Percentage of Biology majors selecting correct choice, reason and combination of
choice and reason for the25 items on the Diffusion and Osmosis Test
Table 1a. Percentage of Male Biology non-majors selecting correct choice, reason and
combination of choice and reason for the25 items on the Diffusion and Osmosis Test
2. The particulate nature and random 2, 3, 6, 16, 18, 47.77 38.40 26.03
motion of matter 22
Table 1b. Percentage of Female Biology non-majors selecting correct choice, reason and
combination of choice and reason for the25 items on the Diffusion and Osmosis Test
Female (N2=136)
2. The particulate nature and random 2, 3, 6, 16, 18, 47.67 37.38 27.45
motion of matter 22
Table 1c. Percentage of Biology non-majors selecting correct choice, reason and
combination of choice and reason for the25 items on the Diffusion and Osmosis Test
2. The particulate nature and random 2, 3, 6, 16, 18, 47.73 37.98 26.62
motion of matter 22
Table 2a. Percentage of male Biology majors selecting correct choice, reason and
combination of choice and reason for the25 items on the Diffusion and Osmosis Test
Table 2b. Percentage of female Biology majors selecting correct choice, reason and
combination of choice and reason for the25 items on the Diffusion and Osmosis Test
Female (N2=280)
CONCEPTS ITEMS Content Reason Correct
choice combination
2. The particulate nature and random 2, 3, 6, 16, 18, 55.24 41.79 32.86
motion of matter 22
Table 2c. Percentage of Biology majors selecting correct choice, reason and combination of
choice and reason for the25 items on the Diffusion and Osmosis Test
2. The particulate nature and random 2, 3, 6, 16, 18, 55.11 42.09 33.33
motion of matter 22