PMID- 31584997

OWN - NLM
STAT- In-Data-Review
LR - 20191004
IS - 1932-6203 (Electronic)
IS - 1932-6203 (Linking)
VI - 14
IP - 10
DP - 2019
TI - Inactivation of chronic wasting disease prions using sodium hypochlorite.
PG - e0223659
LID - 10.1371/journal.pone.0223659 [doi]
AB - Chronic wasting disease (CWD) is a fatal prion disease that can infect deer,
elk
and moose. CWD has now been detected in 26 states of the USA, 3 Canadian
provinces, South Korea, Norway, Sweden and Finland. CWD continues to spread
from
endemic areas, and new foci of infections are frequently detected. As
increasing
numbers of cervids become infected, the likelihood for human exposure
increases.
To date, no cases of CWD infection in humans have been confirmed, but
experience
with the BSE zoonosis in the United Kingdom suggests exposure to CWD should
be
minimized. Specifically, hunters, meat processors and others in contact with
tissues from potentially CWD-infected cervids need a practical method to
decontaminate knives, saws and other equipment. Prions are notoriously
difficult
to inactivate, and most effective methods require chemicals or sterilization
processes that are either dangerous, caustic, expensive or not readily
available.
Although corrosive, sodium hypochlorite (bleach) is widely available and
affordable and has been shown to inactivate prion agents including those that
cause scrapie, bovine spongiform encephalopathy and Creutzfeldt-Jakob
disease. In
the current study, we confirm that bleach is an effective disinfectant for
CWD
prions and establish minimum times and bleach concentrations to eliminate
prion
seeding activity from stainless steel and infected brain homogenate
solutions. We
found that a five-minute treatment with a 40% dilution of household bleach
was
effective at inactivating CWD seeding activity from stainless-steel wires and
CWD-infected brain homogenates. However, bleach was not able to inactivate
CWD
seeding activity from solid tissues in our studies.
FAU - Williams, Katie
AU - Williams K
AUID- ORCID: http://orcid.org/0000-0002-7400-6056
AD - Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories,
National
Institute of Allergy and Infectious Diseases, National Institutes of Health,
Hamilton, Montana, United States of America.
FAU - Hughson, Andrew G
AU - Hughson AG
AD - Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories,
National
Institute of Allergy and Infectious Diseases, National Institutes of Health,
Hamilton, Montana, United States of America.
FAU - Chesebro, Bruce
AU - Chesebro B
AD - Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories,
National
Institute of Allergy and Infectious Diseases, National Institutes of Health,
Hamilton, Montana, United States of America.
FAU - Race, Brent
AU - Race B
AD - Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories,
National
Institute of Allergy and Infectious Diseases, National Institutes of Health,
Hamilton, Montana, United States of America.
LA - eng
PT - Journal Article
DEP - 20191004
PL - United States
TA - PLoS One
JT - PloS one
JID - 101285081
SB - IM
COIS- The authors have declared that no competing interests exist.
EDAT- 2019/10/05 06:00
MHDA- 2019/10/05 06:00
CRDT- 2019/10/05 06:00
PHST- 2019/08/22 00:00 [received]
PHST- 2019/09/24 00:00 [accepted]
PHST- 2019/10/05 06:00 [entrez]
PHST- 2019/10/05 06:00 [pubmed]
PHST- 2019/10/05 06:00 [medline]
AID - 10.1371/journal.pone.0223659 [doi]
AID - PONE-D-19-23738 [pii]
PST - epublish
SO - PLoS One. 2019 Oct 4;14(10):e0223659. doi: 10.1371/journal.pone.0223659.
eCollection 2019.