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WORKSHEET

MICROSCOPE CLASS Biology 101


Cabrillo College

Name __________________________
Section__________________________

The completion of this workbook is necessary for you to receive a grade for this course.
Fill in the sections carefully and hand it in at the end of the class.

First listen to the introduction on Safety presented to you by your instructor. Once the
presentation is over you will have some time to answer these questions that follow.

SAFETY QUESTIONS

1. Where do you dispose of blades and broken glass?

2. a. Where do you dispose of material that has touched human secretions or are
considered a BIOHAZARD?

b. Where do you dispose of biohazard sharps?

3. In case of fire, a flame burning that is under 1 meter2 in area prompts what response?

4. When should you use the safety shower?

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5. Should you wait for an instructor to use the First Aid kit?

6. Why is it important that all food and drink be left outside the lab?

The parts of the microscope will be covered by your instructor. Once the presentation is
over, match the microscope part with its function

____1. Coarse adjustment knob a. Focuses light on specimen


____2. Ocular/Eyepiece b. Moves slide L to R on stage
____3. Mechanical stage w/stage clips c. 40X
____4. Vertical adjustment knob (stage) d. “handle” used to secure scope while
carrying
____5. Iris diaphragm e. Moves the slide forward and back on the stage
____6. Pointer in the eyepiece f. Regulates the amount of light reaching
the specimen
____7. Condenser g. Lens nearest to the eye
____8. Arm h. 100X
____9. Oil Immersion objective lens i. Found in ocular piece, used to point
___10. Horizontal adjustment knob (stage) j. 1000X
___11. Medium power objective lens k. Holds a slide steady and moves the slide
on stage
___12. Low Power objective lens l. Used to focus AT LOW POWER ONLY

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Fill in the blanks:
13. Magnification of the ocular lens is usually ______________________.
14. The lens that provides 100X magnification is the _____________________power lens.




OBSERVING PREPARED SLIDES

LETTER ‘e’
1. The image of the letter e is inverted somehow. What does it look like?

2. Does it matter that all images under the scope are inverted? Explain.

3. What does parfocal mean?

MEASURING
4. Your interpupillary distance (distance between your pupils) for the compounds scope
is____________________.

5. Your diopter adjustment sets the ring at ______________________. (Settings are in the following
range: -2, -1, 0, 1, 2 (This for older scopes only)

COLORED THREADS
6. What is meant by depth of field? (Consult handout if needed)

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7. Color of uppermost thread________________, middle thread_____________, lower
thread_________________.

8. a. What are two ways to adjust light?

b. When would you use one of these adjustments over the other one?

9. Which of the two ways above is most helpful for focusing on the threads?

WET MOUNT
10. Draw the wet mount of Elodea cells at high power (400X). Draw at least 5 cells. Label
chloroplasts and cell walls.

11. Draw 3-4 cells that illustrate what happens when NaCl is added to the leaf. Label the
chloroplasts, the cell membrane and the cell wall.

12. What light adjustment allows for better viewing of the Elodea specimen?

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CHEEK CELLS (This is a biohazard!)

13. Draw cheek cells at 400X (medium power). Draw at least 5 cells.

HUMAN BLOOD SMEAR


14. Draw some red blood cells (10 at least) and two white blood cells. Find a neutrophil and a
lymphocyte.

15. What light adjustment allows for better viewing of the blood cells?

16. There are two kinds of paper commonly used in our Biology labs. Indicate the function of
each.
a. Lens paper _________________________________________
b. Kim wipes _________________________________________

17. At what magnification may you use the coarse (gross) focus? (This question worth 5,000 points!)

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THE CELL CYCLE
Read the description of the cell cycle in the handout before beginning this section. You will be
using Whitefish blastula slides. Use the circles below for your drawings.

18. a. Prophase:
Magnification: _______________
Description: _________________
___________________________
___________________________
___________________________

b. Metaphase:
Magnification: _______________
Description: _________________
___________________________
___________________________
___________________________

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c. Anaphase:
Magnification: _______________
Description: _________________
___________________________
___________________________
___________________________

d. Telophase:
Magnification: _______________
Description: _________________
___________________________
___________________________
___________________________

e. Flag your instructor and show him/her these stages as seen in the onion root tip. Once you
have shown the stages, continue on to the next slide.

MITOSIS CREATES MULTICELLULAR LIFE: A STUDY WITH STARFISH

In the previous exercise you used Whitefish blastula slides to study mitosis. Now you will
examine mitosis at the whole organism scale by looking at how cleavage is reflected in the
development of starfish embryos. Read the handout about the different stages. Draw EACH of
the FIVE stages, labeling your drawings as you did above including what it is, the magnification
used, and any salient anatomical features (see the manual). Also, talk with a lab partner and
answer the questions at the bottom of the next page.

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19. What do you think the blastopore becomes, and why?

20. What happens to the size of individual embryo cells as cleavage continues, and why?

21. What happens to the overall size of the embryo as it transforms from zygote to blastula, and
why?

22. What do you think happens to the weight of an individual embryo as it transforms from
zygote to gastrula, and why?

23. Drawing of cork cells. Follow the directions in the handout and use this area to draw the cell
walls of your cork preparation.

24. Drawing of Ice Plant epidermis. Follow the directions in the handout and use this area to
draw the structure of outermost layer of the plant stem. Label three to four stomata.

25. Drawing of potato slice. Follow the directions in the handout and use this area to draw the
structure of the potato cells. Label the amyloplasts within the cells.

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DISSECTING SCOPE PRACTICE

26. Give an example of when you might want to use a dissecting microscope over a compound
microscope.

Pick an insect provided by your instructor for the class, or one you may have brought in from
home. Place the insect on the stage of your microscope in position such that you can see it
clearly.

27. Wings: Draw the wings of your insect IN DETAIL in the space below. Label three wing
structures as described in the manual on page 24. Make sure you draw all the veins.

Insect Legs and Body: Take a good look at your insect and answer the following questions.

28. Does your insect have a coxa? ____________


29. Does your insect have a femur? ___________
30. Does your insect have a tibia?_____________
If so, does it have spine-like hairs? _______
31. How many sections in your insect’s tarsi? _________
32. Does it have zero, one, or two claws on the tarsae’s tip?
_______
33. Does it have spines on its thorax? _______
34. What color are the eyes? ___________
35. What color(s) is the abdomen? ___________

Insect analyzed: _________________________

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LAB CLEAN-UP

36. What does the colored number taped onto your microscope indicate? Return your scope to
the correct spot.

37. What should tables be cleaned with? When should this be done?

38. Indicate the proper way to return your scope to the shelf.
a. The stage should be ___________________________________.
b. The _________________ objective lens should be in place.
c. The cord should _____________________________________.
d. The light should be __________________________________.
e. The dimmer should be ________________________________.

39. Dis you push your chair in? _______


40. Did you wash and disinfect your desktop? _______

Pack your trash. We hope you have fun in your Biology class!

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