Ozonioterapia como terapia pró-oxidante:

Mecanismos de Ação e Evidências Científicas

O Homem “radical livre”
NIST, National Institute of Standards and Technology
Ozonioterapia
 O que é Ozônio ?

▪ É o quarto oxidante mais potente na natureza

▪ 10 vezes mais solúvel na água que o oxigênio
▪ Altamente instável, logo se recompõe como oxigênio
 Ozônio é um radical livre ?

O ozônio não é uma molécula

radicálica porque possui um número O+
par de elétrons na órbita mais
externa (BOCCI, 2000), porém é
muito oxidante e por isso reage O- O-
instantaneamente com outras
moléculas podendo transformá-las
em radicais livres (RL): ROIs, LOPs,
nutrientes....
Entra Sai

Oxigênio Ozônio
Medicinal Medicinal

SEMPRE
uma mistura
oxigênio-ozônio
 Um bom
ozonioterapeuta com
um gerador de ozônio
ineficiente produz má
Ozonioterapia...
O gás Ozônio

ser inalado !!!

@draemiliaserra
*
@draemiliaserra
 Reação com
antioxidantes plasmáticos

Ativação de células
imunocompetentes e
plaquetas – moléculas de
sinalização – IF, IL, fatores de
crescimento

Ativação do metabolismo das hemácias -

↑ 2,3 difosfoglicerato e G6PDH »
↑ oxigenação tissular

Melhora das condições hemorreológicas

 Sangue
Coração
SNC ozonizado
Glândulas

Pulmões
Ossos

MALT
LOPs Músculos

Baço
TGI

Urina Rins
Fígado Bile
Pele
Os efeitos biológicos imediatos do Ozônio Medicinal
podem ser resumidos...

1- Ativação do metabolismo das hemácias

2 - Ativação das células imunocompetentes

3- Regulação do sistema antioxidante e radicais livres

Antioxidantes
 3- Regulação do sistema antioxidante e radicais livres

León, Bocci et al., 1998

Peralta et al., 1999

70 Protective effect of ozone in

60
Hepatic Ischaemia - Reperfusion
50
40
30 Ativação de enzimas anti-oxidantes e varredores
20
10 de radicais livres - nas células saudáveis
0
SOD GSH
Kontrolle Ischaemia/Reperfusion Ozone+I/R

H2O2
https://www.ncbi.nlm.nih.gov/pubmed/17372841
 Ozonioterapia
- dentro da janela terapêutica -
induz stress oxidativo
leve, controlado e transitório.
https://www.ncbi.nlm.nih.gov/pubmed/9071458
1995
https://www.ncbi.nlm.nih.gov/pubmed/9071458
2017
https://www.ncbi.nlm.nih.gov/pubmed/22583557
https://www.ncbi.nlm.nih.gov/pubmed/25699252
DIRETAMENTE DA TURQUIA

Ozonioterapia em recuperação de trauma raquimedular - em ratos: neste estudo, a

Ozonioterapia acelerou o processo de cicatrização, aumentou a vascularização e
reduziu a lesão neuronal em roedores, sugerindo que a Ozonioterapia pode ser um
tratamento adjuvante em pacientes com lesão raquimedular.

Turk Neurosurg. 2016 May 5.

doi: 10.5137/1019-5149.JTN.17508-16.1.

Effects of ozone on spinal cord recovery via Wnt/ β-catenin pathway following
spinal cord injury in rats.
Tural Emon S1, Uslu S, Ilgaz Aydinlar E, Irban A, Ince U, Orakdogen M, Gulec-Suyen G.
https://www.ncbi.nlm.nih.gov/pubmed/27560537
 Ozônio e Prevenção (León et al. 2002 )
Baixas Doses

Insuflação retal de ozônio em

a modelo animal
Depleção de glicogênio em
células hepáticas
a. Controle
b
b. Depleção de glicogênio
induzida por CCl4

c c. Aplicação preventiva de
ozônio como insuflação
retal (15 sessões)
https://www.ncbi.nlm.nih.gov/pubmed/20626256
https://www.ncbi.nlm.nih.gov/pubmed/22196863
https://www.ncbi.nlm.nih.gov/pubmed/19178770
CONCLUSION:
The results of this study suggests that major ozonated autohaemotherapy could be a safe and
effective therapeutic option for high-risk patients with dry AMD, and that a series of such
treatments could improve the natural course of AMD.
Effects of major ozonated autohemotherapy in the treatment of dry age related macular degeneration: a randomized controlled
clinical study.
Borrelli E1, Diadori A, Zalaffi A, Bocci V. Int J Ophthalmol. 2012;5(6):708-13. http://www.ncbi.nlm.nih.gov/pubmed/23275905
Artif Organs. 2017 Mar 5. doi: 10.1111/aor.12863.
[Epub ahead of print]

Ozone Prevents Cochlear Damage From Ischemia-Reperfusion Injury in Guinea Pigs.

Onal M1, Elsurer C2, Selimoglu N3, Yilmaz M4, Erdogan E4, Bengi Celik J5, Kal O6, Onal O5.

Author information
1Department of Otorhinolaryngology, Konya Educational and Training Hospital.
2Department of Otolaryngology, Selcuk University Medical Faculty.
3Department of Plastic Surgery, Konya Educational and Training Hospital.
4Department of Histology and Embryology.
5Department of Anesthesiology and Reanimation, Selcuk University Medical Faculty.
6Department of Nephrology, Baskent University Medical Faculty, Konya, Turkey.

Abstract
The cochlea is an end organ, which is metabolically dependent on a nutrient and oxygen supply to maintain its normal physiological
function. Cochlear ischemia and reperfusion (IR) injury is considered one of the most important causes of human idiopathic sudden
sensorineural hearing loss. The aim of the present study was to study the efficacy of ozone therapy against cochlear damage caused
by IR injury and to investigate the potential clinical use of this treatment for sudden deafness. Twenty-eight guinea pigs were
randomized into four groups. The sham group (S) (n = 7) was administered physiological saline intraperitoneally (i.p.) for 7 days. The
ozone group (O) (n = 7) was administered 1 mg/kg of ozone i.p. for 7 days. In the IR + O group (n = 7), 1 mg/kg of ozone was
administered i.p. for 7 days before IR injury. On the eighth day, the IR + O group was subjected to cochlear ischemia for 15 min by
occluding the bilateral vertebral artery and vein with a nontraumatic clamp and then reperfusion for 2 h. The IR group was subjected
to cochlear IR injury. After the IR procedure, the guinea pigs were sacrificed on the same day. In a general histological evaluation,
cochlear and spiral ganglionic tissues were examined with a light microscope, and apoptotic cells were counted by terminal
deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining. The apoptotic index (AI) was then calculated. Blood samples
were sent for analyses of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase, malondialdehyde (MDA), the total
oxidant score (TOS), and total antioxidant capacity (TAC). Data were evaluated statistically using the Kruskal-Wallis test. The AI was
highest in the IR group. The AI of the IR + O group was lower than that of the IR group. The biochemical antioxidant parameters SOD
and GSH-Px and the TAC values were highest in the O group and lowest in the IR group. The MDA level and TOS were highest in the IR
group and lowest in the O group. Controlled ozone administration stimulated endogenous antioxidant defense systems, thereby
helping the body to combat IR injury. Although this study revealed a statistically significant decrease in cochlear IR damage following
ozone therapy, further studies will be necessary to explain the protective mechanisms of ozone therapy in cochlear IR injury.

© 2017 International Center for Artificial Organs and Transplantation and Wiley Periodicals, Inc.
https://www.ncbi.nlm.nih.gov/pubmed/28594115
Paravertebral

Intradiscal
*
D 021-0002 11.11.2004
108 Alta Smit _FMD_Nov 2014
Maria Emilia Gadelha Serra
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São Paulo – Brasil

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