Copyright WILEY-VCH Verlag GmbH & Co. KGaA, 69469 Weinheim, Germany, 2010.

Supporting Information for Macromol. Biosci., 2010, 10, 1093 .

Hydrogel Nanofilms for Biomedical Applications: Synthesis via

Polycondensation Reactions

F. Pippig, A. Holländer*

Fluorescence spectra

D
Intensity [kcps]

200

150

100 C

50 B

A
0
450 500 550 600 650
wavelength [nm]

Figure S1. Fluorescence spectra of fluorescamine-labeled amine groups in PEG 2000 diamine GG

hydrogel layers (A: PE + DAE (reference); B: PE + APPG 130 (reference); C: PEG 2000 diamine

GG (35°C); D: PEG 2000 – GG (RT).

10.1002/mabi.201000052
 1000
I [kcps]

800

600

400

200

0
400 450 500 550 600 650
wavelength [nm]

Figure S2. Fluorescence spectrum of dansylcadavarine-labeled carboxylic-acid groups in a PEG

2000 diamine BTC hydrogel layers produced in two reaction cycles (two times coating with PEG

diamine and 30 s reaction each cycle).

6
I [kcps]

0
400 440 480 520 560 600
wavelength [nm]

Figure S3. Fluorescence spectrum of fluram-labeled primary amines in PEG 2000 diamine BTC

network.

10.1002/mabi.201000052
 3.0
4
I [Mcps]

2.5 3

2.0 2

1.5
1
1.0

0.5

0.0
400 450 500 550 600 650
wavelength [nm]

Figure S4. Fluorescence spectra of PEG 2000 diamine BTC hydrogel with an increased carboxylic

acid concentration (PEG 2000 diamine/ alanine ratio: 1: without alanine, 2: 100; 3: 50; 4: 17).

10.1002/mabi.201000052
Calculation of the network mesh size

Based on OH/NH ratio in PEG GG networks

branched unit linear unit

OH

NH

 3V − 3 
Lsum = L0 +   L0
 4 − 2V 
1≤ V < 2

Lsum distance between two branches

L0 length of a branch

V ratio of OH/NH groups

10.1002/mabi.201000052
Model for the calculation of PEG-BTC network composition

C lin + nbranched C PEG

= [C ]
C sum + O sum + N sum
Olin + nbranched O PEG
= [O]
C sum + O sum + N sum
N lin + nbranched N PEG
= [N ]
C sum + Osum + N sum

[X] concentration of the certain element

X amount of the element

nbranched ratio of branched to linear units: 0… 1

{C = C}lin
= [{C = C}]
{C = C}sum + {C − N }sum + {C − O}sum + {CONH }sum + {COOH }sum
{C − N }lin + nbranched {C − N }PEG
= [{C − N }]
{C = C}sum + {C − N }sum + {C − O}sum + {CONH }sum + {COOH }sum
{C − O}lin + nbranched {C − O}PEG
= [{C − O}]
{C = C}sum + {C − N }sum + {C − O}sum + {CONH }sum + {COOH }sum
{CONH }lin + nbranched {CONH }PEG
= [{CONH }]
{C = C}sum + {C − N }sum + {C − O}sum + {CONH }sum + {COOH }sum
{COOH }lin − nbranched {COOH }BTC
= [{COOH }]
{C = C}sum + {C − N }sum + {C − O}sum + {CONH }sum + {COOH }sum

{Y}lin number of atoms of a certain component in a linear unit

{Y}sum sum of the atoms of a certain component

nbranched ration of branched to linear units: 0………1

10.1002/mabi.201000052