Escolar Documentos
Profissional Documentos
Cultura Documentos
Dr. W. McLaughlin
Course outline
l Overview
l History of biotechnology
l Elements of biotechnology
l Genetic engineering: Successes and problems
l Environmental issues
l Trade issues
Overview
l Selection of
transformants on
kanamycin media
l Regeneration of
whole plants by
tissue culture
Regenerate Transgenic plant
Delayed ripening in Tomato
l Introduction of a gene
that results in
degradation of a
precursor of the plant
hormone, ethylene
l Production of tomatoes
for human consumption,
either fresh or processed
l Agritope Inc. USA
Resistance to Colorado potato beetle
l Glyphosate herbicide
tolerance
l Production of Z. mays
for human consumption
(wet mill or dry mill or
seed oil), and meal and
silage for livestock feed
l Monsanto Company,
USA
Papaya Resistant to viral infection
l Resistance to papaya
ringspot virus (PRSV)
l Production of papaya for
human consumption,
either fresh or processed
l Cornell University, USA
& UWI, Jamaica
Virus resistant tomato
l Insertion of a mutant
gene from the virus to
prevent replication
l Production of
tomatoes for human
consumption, either
fresh or processed
l UW-Madison, Hebrew
Univ., UWI
Benefits of the New Technology
l Food safety
l Allergic reaction – Cry9C protein in Starlink
corn
l Use of antibiotic marker genes
l Negative impact non-target species – Bt corn
l Creation of super weeds - gene flow
l Creation of new viruses - recombination
Food safety
l Cartagena Protocol
l CODEX Alimentarius
l National Biosafety Committee-NCST
Cartagena Protocol
l http://www.who.int/fsf/Gmfood/codex_index.ht
m
l http://www.foodstandards.gov.uk/gmdebate/?vi
ew=GM%20Microsite
l http://greennature.com/article299.html
l http://www.doh.gov.uk/cmo/gmfood.htm
l http://www.who.int/fsf/GMfood/FAO-
WHO_Consultation_report_2000.pdf