ECLIPSE E400 Instructions NikonBefore Using ‘Thank you for purchasing this Nikon product. ‘This instruction manual is written for the users of Nikon Microscope ECLIPSE E400. ‘To ensure correct usage read this manual carefully before operating the instrument © {Lis prohibited to reproduce or transmit this manual in part or whole without Nikon's expressed permission, @ The contents of this manual are subject to change without any notice. © Although every effort has been made to ensure the accuracy ofthis manual, if you note any points that are unclear or incorrect, contact your nearest Nikon representative. _— Warning/Caution Symbols in This Manual) > ‘Though Nikon products are designed to provide you utmost safety during use, incorrect usage or disregard of the instructions can cause persona injury or property damage. For your safety, read the instruction manual carefully and thoroughly before usage. Do not discard this manual but keep it near the product for easy reference, Inside this instruction manual, safety instructions are indicated with the symbols shown below. Be sure to follow the instructions marked with these symbols for your safety Symbol Contents A Warning Distexarding instructions marked with this symbol may lead death or serious injury Disregarding instructions marked with this symbol may Iead to injury or eaurion| garding tion y 1 Head to injury property damage, Symbol on the product. ———————— Symbol ContentsLAvrvnine BF toned prose se ‘This microscope should only be used for microscopic observation, Do not use it for any other purpose. Fo nt atassemoie $F cotim ne nut vonage st Nikon representative. If the microscope is used with the wrong input voltage, a short circuit or fire may result, | causing the damage of the microscope. poner cont side) before connecting or disconnecting the power cord, Use only the power cond provided with the microscope. (Refer to p. 24.) Using the wrong power eon could result in damage or fire re Heat rom the light source | the tump becomes exremely bt To vod bas, do not teh he lan adhe sn cond it wile he am is forabout hy minutes arte amp has been red ot Fanner fo avo the isk fire do na place (bie, per or bighyNarmabe mail (uch s psi, pstole sn, pint thinner oalcool near he amp while iis lit or for shout thir’ minutes after it has been tured of The back of the microscope also becomes hot during use, Although this is not a malfunction, be careful not go touch | the back of the microscope when itis hotLorre we ¥ Installation location This product is a preci damage it or may b 1» optical instrument, Using or storing the instrument under unsuitable conditions may ean adverse effect on its precision. The following conditions should be kept in mind when selecting the installation location, © .A.oid installing in a place exposed to direct sunlight, or directly under room lights or any other bright place. The {quality of the view through the microscope deteriorates in bright surroundings © ‘Instat in a place with tle dust and dn. © insta in a level place Free trom vibrations © Lnsta on a sturdy desk oF table tha is able wo bear the weight of the microscope, {© Do not install in a warm and humid location. Otherwise, condensation or moll may form on the lenses, degrading performance or damaging the equipment Check the light source ‘The power supply contained in the microscope provides the power for the halogen lamp as the microscope light Source, Although this power supply can power a halogen lamp of up to 6 V-30 W. the specified lamp must be used Use of a non-specfied lamp could damage the microscope, Specified lamp: 6 V-30 W (PHILIPS 5761), Cautions when replacing the lamp In order to prevent burs allow the lamp to coo (for at leas thinty minutes ater tring off the power before replacing te lamp. Furthermore, to prevent cletical shock and damage o the microncope,aleays tum of the power switch ™ side) and unplug the power cord before replacing the lamp. Alter epcing the imp. be sure to attach the lamp cover securely. Never use the lamp without its cover. Cautions when operating the stage “The stage rack protrudes outward when the stage is moved. ‘When operating the focus knobs or condenser Facus knob, be ‘careful nos to bump your hand on the rack since the end of | the rack may injure your hand ——Lv NM “AUTION ae * Do not wet Spilling water on the microscope may cause electrical short eiuits, resulting the microscope failure or abnormal heat generation. If, by mistake, water or liquid is spilled onthe microscope immediately turn off the power swite (flip it tothe side) and unplug the power cord. Then use a dry cloth to wipe away the moisture. Ifany liquid gets inside (of the microscope, do not altempt to use it: instead, contact your nearest Nikon representative Weak electromagnetic waves ‘This microscope emits weak electromagnetic waves, The accuracy of any precision electronic equipment may be adversely affected if positioned near this microscope. If this microscope affects TV or radio reception, move the radio ‘or TV away from the microscope Notes on carrying the microscope Securely hold onto the microscope with the base of the arm from the back ofthe microscope. Because this microscope is «precision optical device, handle it carefully and do not subject it to strong physical shocks. (The precision ofthe objective in particular may be adversely affected by even weak physical shocks,) Imaddition, do noc grasp the focus knobs, the eyepiece tube, the stage, et., when carying the microscope: there is chance thatthe part could come off, and the microscope could be damaged, ‘When picking up the microscope or puting it down, be careful ‘ot to bump the lamp cover on the back pane! against other thingsNames of Component Parts and Operational Parts Eyepiece tube 4 Eyepiece 4 va ff Diopter Specimen holder adjustment ring = tment ring Condenser clamp screw Eyelevel riser Condenser focus knob jevolving nosepiece Coarse torque adjustment ring (TORQUE) Objective AQUE) — Coarse focus knob < Fine focus knob oN Stage toes nop _ avighnessedustor (ane) Stage rotation \ clamp screw Auto-photo switch Auto-photo voltage selector switchEyepiece tube clamp screw Eyelevel riser clamp screw Coarse focus stopper _ting (CLAMP) Condenser aperture diaphragm ring Fine focus knob Condenser Condenser centering screw Field diaphragm ring a JZ Sao Field lens 4 Power switch Y-axis stage motion ‘control knob X-axis stage motion control knob Rating label Tool holder ‘AC input connector mp cover Power cord_ Back panelProper Use Makes a Difference 1 ae eee eee _s peat iy Effect of Condenser Aperture Diaphragm Stopped down tootar Open too far ‘ ‘ + " Suitable aperture setting " Effect of Diopter Adjustment Proper adjustment Improper adjustment 40Effects of Condenser Focusing and Centering tthe ie diaphagin appears chat inthe conte ofthe view Fe. Hen Comer the sill be co position an esto will be ested Ab Effect of Color Balancing Filter (NCB11) sare seen with yellow tng, Using he 1 vi lamination fom The aap atone. tei in cal when bering specimensy bright > PAB Without using the B11 filter NCBI filer _», singe NEST Me ‘Fetiowish color one ms val daylight colo Effect of Lamp Voltage Adjustment nae is deren the colrone othe light econ reer, Conversely ifthe vole is meres the Ieahe lamp Low lamp voltage Ee Reiksh cerone AonwoContents —— — - = = Before Using ——_______—___— 1-4 Names of Component Parts and Operational Parts — 67 Proper Use Makes a Difference to-tt Contents. 8 Preparations for Microscopy 14 Placing a Specimen on the Stage 15 Changing Magnification 16 Photomicrography — 17-48 Locking the Focus Position 19 Changing the Torque of the Coarse Focus Knob— 20 Changing the Torque of the Stage Motion Control Knobs ———— 20 Selecting a Condenser 21-22 ‘Assembling and Disassembling the Microscope — 23-25 Replacing the Lamp - 26 Installing the Condenser 27 Installing the Stage — ——— 28 Installing the Objectives 28 Installing the Eyelevel Riser (If necessary) 29 Installing the Eyepiece Tube. ——— 29 Installing the Eyepieces 30 Lens Cleaning 30 Keeping the Microscope Clean —————_ 30 Storage at Regular Inspections ————_——_—— 31 Troubleshooting Tables — 32-34 Specifications and Ratings— 35 Index — 26St Preparations for Microscopy ot brightness adjuster to a suitable brightness ae © Dicpter and interpupillary Distance Adjustments These adjustments are performed to adjust the microscope tothe person sing it. Continued use ofthe microscope without propery ajo dtr anita dtnce can caus eye sain. Make sure o asthe microscope propel whenever sou use it, opterajusnent compensates fr differences in vision between the et and ight exes. In ation to making “observation through both eyes easier, this adjustment also reduces the defocusing when changing the objective. Perform diopter adjustment for both eyepieces, respectively. 341 Tum the diopter adjustment ring onthe eyepiece to align the edge ofthe diopter adjustment ring with the engraved Tine, (This isthe diopter adjustment 0" position.) The diopter adjustment “0° position 9-2 Turn the coarse and fine focus knobs to focus on the specimen with the 40x objective. (When itis difficult to focus on the specimen, first focus using the 4X or 10% objective, and then switch to the 40x objective.) Since the distance betwen the objective tp an the top ofthe cover glass is indicated us the working distance (WD) on the objective barrel refer to that distance when focusing on the specimen. 348 Move the dor 10x objective ito the optical path. Tum the diopter adjustment ring on the eyepiece not the coarse and fine focus knobs, to focus on the specimen. Do so ‘while peering through the right eyepiece with your right and the left eyepiece with your let eye, 34 Adjust the spread of the binoe part tothe distance ‘between your eves so thatthe field of view for each eye is aligned on one positon when viewing with both eyes,ee Placing a Specimen on the Stage ee the cover lass increased, the color tone becomes bluer.Cor Changing Magnification Adjustment of condenser aperture diaphragm Adjust the ciaphragm coring to the objective numerical aperture. Turning the condenser aperture diaphragm rng changes the sizeof the aperture diaphragm. AS the aperture diaphragm is stopped down, brightness reduces and the details of the specimen become more difficult see, while contrast and depth of Focus ine ease. Conversely. 38 the aperture diaphragm is, ‘opened, brightness increases and the details hecome easier to se, while contrast and depth of focus reduce, Is not possible to ‘adjust one pair of characteristics without affecting the other. Gent aly. a well-defined image with appropriate contrast ean be biained with an aperture setting tha is 70% to 80% of the objective numerical aperture. The numerical aperture of each objective is indicated onthe barrel of the objective, IF the aperture daphragn is tapped down oo Far speinen | Plag.aox an ; ston aged os? FEE merical apenure: 0.35] low such as when viewing # ne ly aI transparent specimen, do not stop down the aperture 10 less thn (60% of the numerical aperture of the objective © Adjusting the size of the aperture diaphragm according to the condenser scale Because the condenser scale indicates the numerical aperture... adjust the apertare diaphragi ring aeconding to the scale 0.6 Objective with N.A, 078 0.75 0.3 400.8) = 05: Adjust Ato this value, | © Adjusting the size of the aperture diaphragm by using a centering telescope (sold separately) Remove one of the eyepieces and, using a special adapter (sold separately). mounts centering telescope in its place. Turn the aperture diaphragm 10 stop down the aperture diaphragm as Far as possible, While holding down the flange of the centering telescope. tum the eyepicce othe telescope to facus on the aperture diaphragm, Turn the aperture diaphragm ring ‘oadjust the size of he aperture diaphragm. Remove the centering telescope and the adapter, and then reinstall the eyepie Adjustment of field diaphragm The Field diaphragm Fimits illumination to the ares of the specimen being viewed to prevent excessive Hight fam entering the view field, Turing the field diaphragm ring changes the size ofthe tcld diaphragm. For normal observation. the size of the diaphnigim should he just outside the elgeof the view Field This adjustment can produce the image with appropriate contestA+ Photomicrography Fenn nnn ee ne a i {© Refer to the instructions of the photomicrographic equipment being used for detailed information on the assembly method and pphotomicrographie procedure. @ Sclecting an eyepiece tube A ticular eyepiece ube equiped with a vortcal be is used, There are to type fino eypiee tubes, Sees he tue acorn othe purpose of Operating the optical pat election lever changes the amount of ight entering the binocular part and vertical tube af the ‘eyepiece tube as shown below F eyepiece ] Lit epic Teyepiece tube ever Bir este pot * ser Bier Vert (im weno. stn pat be Fam Brot 100 T pO 6 AM RSE -@ 2 0 ames locrte | a =} ] on aN Il 1-4 Vertical tube adapter [A photo vertical tube adapters provided tothe tinge |= $$$ eyepiece tube as standard equipment, It allows a | Ts¥ePiece te [ Feyepiece tube eyepiece tube as standard equipment. It all Pa. hotomierographic equipment to be installed. To install the photo vertical tube adapter, insert it into the vertical tube und tighten three clamp screws onthe vertical tube using the hexagonal screwdriver provided. Replace the Photo tube adaper with the TV vertical tube adapter (sold separately) in the case of using a TV camera, 4-2. Release of T and F eyepiece tubes lever pare Re aoeea NO CLICK” switeh is used for minimizing sight 4 Vibrations produced by operation of the optical path selection lever. This switch is equipped on the bottom (installation) surface of the eyepiece tube. Turn the ey ke switeh in the arrow direction sing the hexagonal screwdriver provided, and the “NO CLICK" will become efetiveto remove the clicking action ofthe optical path© selecting color tone and brightness The color tone ofthe lamp varies according Wo the vole. If the volage increased the olortne ofthe np Becomes ber Ifthe vollge i deceased, the color tone ofthe lamp becomes redder. Therefor. n onrto dan the es color rerodution in color photomierography, the lamp voltage should be kept ‘constant. When using daylight-type color film, the standard setup is to use the color balancing filter (NCB11) and set the lamp voltage 1 the standard for photomicrograph. The auto-photo switch is used to automatically set the Standard lamp voltage. IFthe image on colo fil shot with the aulo-photo ‘witch pashed are reddish or bluish, finely adjust the voltage with the auto-photo voltage selector switch, The center position of the $-tevel slide switeh i the standard position. Sliding the switch forward increase the blush int ofthe Hight, whi siding the sist towards the ack increases the reddish tint ofthe ight. If this adjustment is not enough to resolve the problem, aso use commercially available color compensation fikers (CC filters). © trimming Loosen the stage rotation clamp screw in onder to rotate the Mage. Tighten the clamp screw at the desired position and secure in| position |@ coarse focus stopper Once the coaneficus toppers clamped in pla, he oa foes kao cannot welto moe the stage any higher (Movement of he st by the fine focus knob is not restricted.) In effect, once the coarse facus knob is clamped in place atthe focus Position, a rough focus ean be attained the next time simply by turning the coarse Focus knob as far as it will go. This feature is convenient when viewing similar specimens one alter another © Using the coarse focus stopper -—————— With he specimen infocus. tu the coarse focus topper ring as fart wil go (about 270°) in the arrow direction indicated on the microscope base ie, clockwise dretion). The coarse focus stoppers now clamped in place When changing the specimen, lower the stage by tuning nly the coare focus knob Alter ehanging the specimen, gently raise the stage by turning en the coarse Focus knob as far it wil go, ‘The specimen should be roughly in focus when the stage has heen raised as far as it will go; use the fine focus knob to bring the specimen into perfect focus I the coarse focus stopper is not being used, be sure to loosen the coarse Focus stopper ring as far as i¢ will zo (by tarning i as far as i will in the opposite direction of the arrow indicated lon the microscope base). 19,, Changing the Torque of Coe the Coarse Focus Knob Beene The truco the corse fous kab canbe aust Toincrease het, tu he coune oe adjtnent ig (TORQUE) : es Increase located behind the eoarse focus knob in the arrow diction indicted on <3 ao the microscope hase (ithe couner-ockwibe deton. To ede the torque, ur the ing inthe opposite diction ofthe arow Ge elockvise dieton © Continuing to rotate the coarse focus knob after the stage has reached the limit of its motion, ,, Changing the Torque of Cor the Stage Motion Control Knobs —— + The torgue ofthe X-axis and Y-axis stage motion control knobs can each be adjusted, @ Adjustment of torque of Y-axis stage motion Totihten te tension nthe ans tum Knob Bin te dietion of row "a" while holding Knob A; to reduce the tesion, tar Knob Bin the opposite diretion @© Adjustment of torque of X-axis stage motion Totighen he tension in the X ais, tm Knob Cin the direction of artow "b” while holding Knob D: to reduce the tension, turn Knob C in the opposite direction.Cor Selecting a Condenser Pa oe 2 seroma | gvingout | Actomet tbo Low jective ing cu oma pana rmagteaton naga cian! | congenscr | condenser | condenser | “aonvcae x % x x | x x x © evo?) (ore) ; x oe | oe 10-10 100: ° x SOpiman Appropriate” — > appropri Note 1: The entire field of view may not be covered when a UW eyepiece is used [Note 2: Indoor li 1 and similar light sources may be reflected off the surface of the condenser lens and be visible within the field of view. When tis happens either dim the indoor lighting or prevent strong outside ight from entering ont the sage. Note 3: Swing out the 1p lens © Depending on the type of objective, the whole numerical aperture of the objective may not be covered. For example, when an objective with N.A. 1 is used in combination with a ywing-out condenser or abbe condenser, the aperture size will only be about 65% ofthe objective's N.A. even when its opened to its Kimi © For the universal system condenser and phase contrast condensers, refer to each instruction manual @ itimmersion operation (using an oil-immersion type objective and condenser) Objectives marked “Oil” are oil-immersion type objectives. ‘These objectives are used with the provided immersion oil applied between the specimen and the tip of the objective. In order to get maximum performance from oil-immersion type objectives with « numerical apertute of 1.0 or greater, an oil immersion type achromatic aplanat condenser must also be used. COl-immersion type condensers are used with of applied between the specimen and the condenser lens. Bubbles i the oil will adversely affect the viewing of te image, [__"_S\ 4 WQuet Be careful to peevent the formation of air bubbles, To check for aie bubbles, remove the eyepieces, open the field diaphragm and the aperture diaphragm as far as possible and look at the exit pupil of the objective within the eyepiece tube, (The exit pupil will appear asa bright circle) If itis difficult to see if there are any bubbles, mount a centering telescope (sold separately) on an veri ce sleeve by using an adapter (sold separately). Then, while turning the eyepiece on the centering telescope to change the focus, look througth the centering telescope for air bubbles, [there are bubbles in the oi, remove them by one of the folowing, methods: © Tum the revolving nosepiece slightly to move the oil-immersed objective back and Forth one or two times. ‘nthe case of the condenser, gently turn the condenser focus kaob to move the condenser up and down slightly.) © Add more oil ‘© Remove the oil and replace it with new ol= © Use us litle oi as possible (ust enough to fill he space between the tip of he objective and the specimen. or beteen the tip cof the condenser and the specimen). 1ftoo much ol s applied, the excess will flow onto the stage and around the condense. © “0, Problem Cause *-~\ Countermeasures “The power supply is not plugged in Plug the power cord into an outlet. omy “Te power cord snot connected tothe ‘Connect the power cord ay “The lamp does not ight | microscope whan ne power wich Tye np has at een alld Tosa he lamp. 726) “The Lamp s burned out Replace the lamp. (726 “The speci lamp i not being used Use the specified Kamp, (926) ‘The lamp is near the endo is i Replace the lamp. (26) cre erenor 1 connected secur secure the camnecton peeouea cee “The power cord is not connected securely Secure th 24 “The lamp is nox plugged into its socket securely. | Insert the lamp securely ino its socket. (926) eeSpecifications and Ratings 1 | Model Name: ECLIPSE E400 (microscope mai body) 2 | Focusing Mechanism © Fine focus koh sake: 1 step equals | ym {© Amount of movement of fine focus knob (One turn moves the sage up or down by 0.1 mm © Amount of movement of course focus hnob: One tra moves the stage up or down by about 12 mm © Range of sage veil motion 2 mm up and 28 mm down rom the reference (focused) positon 3 | Stage © Provided with knob torque adjustment mechanism (© Provided wih coation mechanism 4 | Revolving Nosepiece © Locking type with Five-holes 5 | illumination © txorsal Koebler‘ypediascopic illumination opis © Provided with a PHOTO switch (S-level fine adjustment selector) © Lamp rating: 6 V DC, 30 W halogen lamp, PHILIPS $761 6 | input Power Supply Voltage © AC 1n0VH120V + 105 soe Curentconsmption: 08 oss Ineral fine ing: 250V, T2A © 5C230V 510% suse Curent consumption: 4 or less Intra fine rating? —-250V, TRA 7 | Protection Class © chat 8 | Operating Environment © Temperaue: Oto. H40rC (© Humidity: Relative amity max. 85%, non-condensing © stitute Max, 2000 m © Potton Degree? © nsllaion: Category It © Indoor se only 9 | Conforming Standards © Praduct with AC 120 V + UL Liste product + ROC TSI Clans satis This equipment hs hen teste and found o comply with helms foe Cas ital device pursuant o Par IS ofthe FCC Rules These nis are designed to provide reasonable protections against harmful inteserence when the open isapeatd ina camel environment. This equipmest generates, uses, nd ean radiate radiofrequency energy and if ot nse and used in accords with ‘he instucton manual. may cause haraful interference to radio communications. Operation of thi equipment na reside re is ikely to cause harm ntererece in which the wer wil erated to correct he interference a this own expense “This Class A gil apparatus meets al requirements ofthe Canadian Intrferenoe-Cavsing Equipment Regulations, Cet apparel numérique de lu Case A respecte totes es exigences da Réglement sr le matériel bruileur dy Canad, © Product with AC 230 V * GS approved prouct + EU Low Voliuge Directive satisfied + BU EMC Directive sasied ————Index ey {© Absolute aleoho $$$ __________ 2,30 (© Aperture diaphragm to. ts © Aperture diaphragm ring —______-— 16 © Auto-photo switch 11,18 © Contering telescope 16 (© Coarse and fine focus knobs “4 (© Coarse focus stopper $9 {© Color balancing fiter (NOB 1) — ttt {© Color compensation (CO) fiter 18 © Color fi 18 © Color tone $$ 11,18 © Condenser focusing and centering $$ ______________ n.27 © Contrast 10,18 © Dioptor adjustment 10,14 © Diopter adjustment ring — OE ) (© Field diaphragm 16 @ Input voltage, ats © ‘Installation location § © Interpupiliary distance adjustment $$___________________4 © Lamp vottage 11,18 © Numerical aperture 10,16 © OiLimmersion 21 ‘© Petroleum benzine 22,30 (© Photo vertical tube adapter, 7 (© Power cord tt (© Power switehy ei © Release of T and F eyepiece tubes lever clicking 7 ‘© Found dovetail 29 © Spocited lamp 3,26 © Specimen holder 2 © substage 28 (© Torque adjustment $29 {© Trinocular eyepiece tube 7 © Trimming 8 © TV vertical tube adapter 7 (© Water-immersion§ $$$ (© Wiping of 9 $$$ 22,30 — — ~ ed