Escolar Documentos
Profissional Documentos
Cultura Documentos
29. G. Chang, R. Spencer, A. Lee, M. Barclay, D. Rees, data 39. S. Bailey, CCP4 Project, Acta Crystallogr. D 50, 760 P. C. Moe from the University of Wisconsin–Madison for
not shown. (1994). several cloned mscL homologs and for the E. coli knock-
30. A. Kreusch, P. J. Pfaffinger, C. F. Stevens, S. Choe, 40. Single-letter abbreviations for the amino acid resi- out mutant for mscL, A. Okabe of the Kagawa Medical
Nature 392, 945 (1998). dues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, School (Kagawa, Japan) for providing cloned DNA of
31. X. R. Ou, P. Blount, R. J. Hoffman, C. Kung, Proc. Natl. Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; mscL from C. perfringens, and D. Dougherty and H.
P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Lester for helpful discussions. We also thank the staff at
Acad. Sci. U.S.A. 95, 11471 (1998).
Y, Tyr. the Stanford Synchrotron Radiation Laboratory (SSRL)
32. P. Blount, S. I. Sukharev, M. J. Schroeder, S. K. Nagle, 41. J. D. Thompson, T. J. Gibson, F. Plewniak, F. Jeanmou- and the Advanced Light Source (ALS) for their help in
C. Kung, ibid. 93, 11652 (1996). gin, D. G. Higgins, Nucleic Acids Res. 25, 4876 (1997). data collection. The synchrotron rotation camera facil-
33. C. C. Hase, A. C. LeDain, B. Martinac, J. Membr. Biol. 42. publ.
R. M. Esnouf, J. Mol. Graphics 15, 132 (1997). ities are supported by the U.S. Department of Energy
First in: Science 282 (1998), pp. 2226-2230
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157, 17 (1997). 43. P. J. Kraulis, J. Appl. Crystallogr. 24, 946 (1991). (ALS and SSRL) and NIH (SSRL). G.C. and R.H.S. were
34. , J. Biol. Chem. 270, 18329 (1995). 44. E. A. Merritt and D. J. Bacon, Methods Enzymol. 277, supported by NIH postdoctoral fellowship grant
35. W. J. Sigurdson, S. I. Sukharev, C. Kung, F. Sachs, 505 (1997). GM18486 and an Amgen postdoctoral fellowship, re-
Biophys. J. 72, WP104 (1997). 45. B. Honig and A. Nicholls, Science 268, 1144 (1995). spectively, during the initial stages of this project. Sup-
36. H. A. Lester, Annu. Rev. Biophys. Biomol. Struct. 21, 46. We thank A. Chirino for advice and computer sup- ported by the Howard Hughes Medical Institute. Protein
267 (1992). port, J. G. Spencer for technical assistance, S. Gordon Data Bank identifier for Tb-MscL is 1MSL.
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37. N. Unwin, Nature 373, 37 (1995). of the Institut Pasteur (Paris, France) for his kind gift
38. , J. Mol. Biol. 257, 586 (1996). of genomic DNA from M. tuberculosis, C. Kung and 29 October 1998; accepted 16 November 1998
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