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Rao MD
Importance of Fungal Infections
.
Only a small amount of material is required
Inoculate one edge of the agar square with
the fungus to be examined
Inoculating the agar block.
Agar block with all
four sidesinoculated
Sterilize the coverslips by immersing
them in alcohol.
Picking up a cover-slip with forceps
Using sterile forceps, remove and flame a
coverslip to burn off the alcohol and place it
on top of the agar block.
In slide 4 The coverslip being placed on a slide culture
Add a little sterile water to the base of the
Petri dish to ensure that the agar does not
dry out while the culture is incubating
The duration and temperature of the incubation depend
upon the species of fungus being examined
Removing the cover-slip and its growth from a slide
culture
When sufficient growth has occurred.. lose-up of
cover-slip on a clean slide, fungal growth
uppermost.
Place the coverslip, with the fungal growth
uppermost, onto a clean slide. A drop of alcohol
being applied to the fungal growth on the cover-slip
Add a drop of 70% alcohol to the center of
the coverslip.. Drop of lacto phenol cotton
blue on the coverslip.
Placing a stained slide culture onto a
clean slide Turn the coverslip over and
place it onto a clean slide.
Morphology of Chlamydospores
How the Chlamydospores look
Microscopic appearance of the slide culture at room temperature
(Czapek-Dox solution agar) after 2 weeks. Conidia in a chain with
transverse and oblique septa were observed.
The MycoVue™ System
provides the laboratorian with
a standardized, comprehensive
method that eliminates time-
consuming preparations and
technical difficulties
encountered with the classical
slide culture technique. It
simplifies the slide culture
method by providing all the
necessary components for this
procedure in one ready-to-use
disposable unit.
MycoVue™ System
The system comes complete
with a protective lid and a
built-in humidifying chamber.
The device is designed to fit
easily onto a microscope stage,
thereby allowing direct
viewing of the developing
fungus through the device,
thus eliminating the disruption
of the fungal colony. If desired,
the cover slip can be removed
and stained for further
evaluation or preservation.
References
Microbiology Teaching Support Faculty of Biological
Support University of Leeds
Created by Dr.T.V.Rao MD for ‘e’
learning resources for Medical
Microbiologists and Mycologists in
the Developing world
email
doctortvrao@gmail.com