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IV B.Tech II Semester Supplimentary Examinations, May 2008
CHROMATOGRAPHIC SEPARATIONS
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
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2. Describe one method for the development of chromatogram and write a note on
column resolution. [16]
3. Briefly outline the advantages which may be gained by preparing specific ion ex-
change resins which contain functional groups known to react selectively with par-
ticular groups of cations. [16]
(a) PLOT
(b) Chromatogram
(c) Electron capture detector
(d) Properties of mobile phase in GC. [4×4=16]
7. (a) Write about the types and interactions between chiral resolving agent and the
solute.
(b) Write the industrial applications of chiral chromatography. [8+8]
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Code No: RR422301 Set No. 2
IV B.Tech II Semester Supplimentary Examinations, May 2008
CHROMATOGRAPHIC SEPARATIONS
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆
⋆⋆⋆⋆⋆
1 of 1
Code No: RR422301 Set No. 3
IV B.Tech II Semester Supplimentary Examinations, May 2008
CHROMATOGRAPHIC SEPARATIONS
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆
1. Classify and explain the chromatographic techniques with suitable examples and
schematic diagrams. [16]
2. Describe one method for the development of chromatogram and write a note on
column resolution. [16]
3. Briefly outline the advantages which may be gained by preparing specific ion ex-
change resins which contain functional groups known to react selectively with par-
ticular groups of cations. [16]
5. What is the design principle of atypical HPLC machine? Discuss with suitable
equation. [16]
6. Write about the stationary phases commonly used in Gas chromatography. [16]
8. Write about electro osmotic flow and pressure induced flow in capillary electrophore-
sis. [16]
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Code No: RR422301 Set No. 4
IV B.Tech II Semester Supplimentary Examinations, May 2008
CHROMATOGRAPHIC SEPARATIONS
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆
2. Differentiate between the photouiode array detector and fluorescence detector with
suitable diagrams. [16]
3. (a) List out the commonly used ion exchangers and write matrices used for them.
(b) What are the factors that influence the ion exchange chromatography perfor-
mance? [8+8]
7. (a) What are various chiral mobile phase additives used in chiral chromatography?
(b) Write the principles separation of racemic mixture in chiral chromatography.
[8+8]
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