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Isolation and Analysis of DNA from White Onion

(Scientific Paper)

Submitted by: Jan Iña Lamberte, Renz Lester Laudato, Rona Mallari, Ruby Rose Marfil,
John Nicholo Katipunan

De La Salle Health Sciences Institute

Dasmariñas, Cavite
College of Nursing and Midwifery

This experiment allowed us to determine the nucleotides present in the DNA of an onion
through isolation subjected to chemical characterization tests. DNA was characterized
through the Dische Reaction: Test for Deoxyribose, Test for Phosphate and Murexide
Test for Purines. The first test, which is the test for deoxyribose resulted a purple
solution, the test for phosphate resulted a yellow precipitate, and the test for phosphate
gave us a yellow-green solution.

Deoxyribonucleic acid (DNA) is the genetic material of all organisms. It is the

master molecule in whose structure is encoded all information needed to create and
direct the chemical machinery of life (Micklos and Freyer, 1999).

To most students, DNA is an abstraction. We can memorize the names and

structures of the nitrogenous bases and know all about the history of DNA's discovery,
but until you actually handle DNA, it remains a strange and mysterious substance. DNA
is the hereditary material in humans and almost all other organisms. Nearly every cell in
our body has the same DNA.