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Extraction characteristic of bovine serum

albumin using sodium bis(2-ethylhexyl)
sulfosuccinate reverse micelles View Record in Scopus

Koichiro Shiomoria, , Naofumi

a a
Ebuchi , Yoshinobu Kawano , Ryoichi
b b
Kuboi and Isao Komasawa
a
Department of Materials Science,
Miyazaki University, Miyazaki 889-2155,
Japan
b
Department of Chemical Science and
Engineering, Graduate School of Engineering Science, Osaka University,
Toyonaka 560-0043, Japan
Received 6 May 1998; accepted 18 September 1998. ; Available online 31 March
1999.

Abstract
The extraction of bovine serum albumin (BSA, M. W. 66 kDa, pI 4.9) was
carried out using a reverse micelle consisting of sodium bis(2-ethylhexyl)
sulfosuccinate (AOT). When CaCl2 or MgCl2 was used, BSA could be extracted
at a pH higher than the pI of BSA. This effective extraction at a pH higher than
the pI was considered to be caused by the modification of interactions between
the protein and the micelles by the divalent cations. For KCl and NaCl, BSA
could be extracted only at high concentrations of AOT and within a narrow pH
range, and maximum extraction was achieved at the pH values of 5.5 for NaCl
and 5.0 for KCl. The BSA extraction efficiency decreased sharply at high
concentrations of NaCl and KCl. Sufficient concentrations of AOT in the
organic phase, which depends on the salt type and its concentration, are
required to extract BSA into the organic phase. In the range of insufficient
concentration of AOT, aggregation and precipitation of BSA with AOT occurred
in preference to extraction. BSA extracted into the organic phase could be
back-extracted into a fresh aqueous phase at high pH values and high salt
concentrations. The conformation of BSA was maintained to a large extent
during the extraction process.

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ScienceDirect - Journal of Fermentation and Bioengineering : Extraction c... http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T8G...

Author Keywords: reverse micelle; extraction; protein; albumin; BSA; AOT

Article Outline

• References

Corresponding author.

Journal of Fermentation and Bioengineering

Volume 86, Issue 6, 1998, Pages 581-587

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