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1 of 3 3/26/2011 8:21 PM
How to Measure Cell Viability | eHow.com http://www.ehow.com/print/how_6114019_measure-cell-viability.html
Difficulty: Moderate
2 of 3 3/26/2011 8:21 PM
How to Measure Cell Viability | eHow.com http://www.ehow.com/print/how_6114019_measure-cell-viability.html
Counting dead cells can be subjective, depending on what the technician considers a
blue cell (the intensity of the blue color may vary depending on how much was
absorbed into the cytoplasm). Therefore, it is wise to do a viability measurement with at
least two separate samples and obtain an average. Alternatively, the technician could get
another person to also perform the count and compare results.
Use personal protective equipment when performing this assay, such as gloves and a lab
coat. According to the Material Safety Data Sheet (MSDS), trypan blue may cause
cancer, so practice appropriate laboratory safety methods.
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