Analyze 12 Cytokines or Chemokines Simultaneously Using ELISA

Multi-Analyte Profiler ELISArray™ qualitatively profiles a panel of cytokines and chemokines in multiple samples at the
same time. They are designed to survey a specific panel of cytokines or chemokines involved in autoimmunity,
inflammation, or T cell biology in cell culture supernatant, serum or plasma.

Features
● ● ● ●
 Multi-Protein Flexibility : Detect up to 12 cytokines or chemokines at once using the same incubation time.
 High Performance : Profile with high sensitivity and validated capture and detection antibodies.
 Ease of Use : No special equipment is required, only the ELISA plate reader already in your lab.

Negative Control Cytokines Antibody Antibody Antibody

Combination 1 Combination 2 Combination 3
1 2 3 4 5 6 7 8 9 10 11 12

X
Capture Antibody A Capture Antibody B Capture Antibody A
A GM- Detection Antibody C Detection Antibody C Detection Antibody D
IL1α IL1β IL2 IL4 IL6 IL8 IL10 IL12 IL17A IFN TNF CSF
GM- Failed Due to Passed On All Failed Due to
B IL1α IL1β IL2 IL4 IL6 IL8 IL10 IL12 IL17A IFN TNF CSF
High Background ELISArray QC Criteria Poor Linearity
C IL1α IL1β IL2 IL4 IL6 IL8 IL10 IL12 IL17A IFN GM-
TNF CSF 4.0
Samples

GM-
D IL1α IL1β IL2 IL4 IL6 IL8 IL10 IL12 IL17A IFN TNF CSF
GM-
E IL1α IL1β IL2 IL4 IL6 IL8 IL10 IL12 IL17A IFN TNF CSF 3.0
GM-
F IL1α IL1β IL2 IL4 IL6 IL8 IL10 IL12 IL17A IFN TNF CSF
OD 450

GM-
G IL1α IL1β IL2 IL4 IL6 IL8 IL10 IL12 IL17A IFN TNF CSF 2.0
GM-
H IL1α IL1β IL2 IL4 IL6 IL8 IL10 IL12 IL17A IFN TNF CSF

Antigen Standard Control 1.0

Layout of Cataloged ELISArrays（Cat# MEH-004A） : The ELISArray plates are made
up of twelve 8-well strips. Each strip is coated with a capture antibody for its target
cytokine. One well of each strip is dedicated to the analysis of a single biological 0
0
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1000
0
125
250
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1000
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1000
sample. You can profile the levels of your twelve target analytes in eight samples per
plate with a single ELISArray Kit experiment.

IL-10 Antigen (pg/mL)

The ELISArray optimization process is demonstrated by the three examples of
antibody combinations screened for the IL10 analyte. The antigen standard
Components Provided curves from the best performing capture and detection antibody concentra-
tions for three combinations are shown. Only the combination of antibodies B
and C passes all three rigorous quality control riteria (Antibody Combination 2).
Avidin-HRP Conjugate, 10% BSA, Donkey Serum, 96-well pre-coated The other two combinations produce too high a background (Combination 1) or
Capture Antibody microplate, Detection Antibody Dilution Tube Strip, a response that is not linear enough (Combination 3).
Sample Dilution Buffer Stock, Assay Buffer Stock, Wash Buffer (10X Conc),
Development Solution, Stop Solution. Our assay development team is not dedicated to one
antibody supplier. We systematically screen for the best
We provide everything you need for ELISA. capture and detection antibody pairs available on the
market.

Product Description Organism Cat #

Human

Mouse

Human

Mouse

Human

Mouse

Human

Mouse

Genomax Technologies Pte Ltd Tel: (65) 6777 1886 Email: info@genomax.com.sg