WARRIORMAX

Code No: K2301/R05

Set No. 1

IV B.Tech. II Semester Supplementary Examinations, July 2010

ANIMAL CELL SCIENCE AND TECHNOLOGY
(Bio-Technology)
Time: 3 Hours Max Marks: 80
Answer any Five Questions
All Questions carry equal marks
*******
1. Write notes on
i. Growth curve of mammalian cells
ii. Haemocytometer

2. Write notes on
i. Protein free medium
ii. Buffering system in cell culture

3. What is the importance of inverted microscope & Bio safety cabinet used in animal cell
culture. Explain the types of BSC s available & arrangement of parts in inverted
microscope

4. Write notes on
i. Enzymatic disaggregation
ii. Subculture of a monolayer

5. Explain about different transformation methods

6. Differentiate embryonic & adult stem cells. What is the role of embryonic stem cells in
research?

7. Write notes on
i. Differences between organ and histotypic cultures
ii. Microcarrier culture

8. Explain about tissue engineering and its application





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Code No: K2301/R05
Set No. 2

IV B.Tech. II Semester Supplementary Examinations, July 2010

ANIMAL CELL SCIENCE AND TECHNOLOGY
(Bio-Technology)
Time: 3 Hours Max Marks: 80
Answer any Five Questions
All Questions carry equal marks
*******
1. What are the different physico chemical properties maintained in a culture medium?

2. Explain the layout of a small scale cell culture laboratory. List out the essential
equipments used in cell culture.

3. Write notes on

i. MTT assay
ii. Karyotyping

4. Write in detail about three dimensional culture

5. Explain about different cell separation methods

6. Write about

i. Chemical methods for cell synchronization

ii. Somatic cell nuclear transfer

7. Write in detail about different mechanisms involved in programmed cell death

8. Discuss in detail about

i. Monoclonal antibodies
ii. Cell culture based vaccines

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Code No: K2301/R05 Set No. 3
IV B.Tech. II Semester Supplementary Examinations, July 2010
ANIMAL CELL SCIENCE AND TECHNOLOGY
(Bio-Technology)
Time: 3 Hours Max Marks: 80
Answer any Five Questions
All Questions carry equal marks
*******

1. Write in detail about insitu & exsitu molecular mechanisms

2. Enumerate are the constituents in growth medium & discuss the role each constituent

3. Write in detail about

i. What is ‘Vero’ cell line? Make differences between primary and established cell
lines
ii. Balanced Salt Solutions

4. What is the need for characterizing a cell line? Explain any two methods to characterize a
cell line.

5. Discuss in detail about

i. Hybridoma technology
ii. List out the vaccines made by cell culture

6. Write in detail about tissue engineering

7. Write in detail about

i. Centrifugal elutraiator
ii. Micromanipulation

8. Write down the applicative part of animal cell culture.

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WARRIORMAX
Code No: K2301/R05 Set No.4
IV B.Tech. II Semester Supplementary Examinations, July 2010
ANIMAL CELL SCIENCE AND TECHNOLOGY
(Bio-Technology)
Time: 3 Hours Max Marks: 80
Answer any Five Questions
All Questions carry equal marks
*******
1. What is serum containing medium? Enumerate the advantages and disadvantages by
using serum in culture medium

2. What is the role played by CO2 incubator, LAFU, inverted microscope & Liquid N2 in
cell culture laboratory

3. What is cell cytotoxicity? How cytotoxicity can be measured?

4. Explain about different disaggregation methods used in cell culture

5. Write in detail about somatic cell genetics and discuss its role in research

6. Write about different types of organ culture

7. What is tissue engineering? How it is playing a major role in research

8. Write about

i. “The main problem in cell culture is contamination” enumerate the statement

ii. FACS



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