Instruct_sht.

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Instructions for Use: Weekly biological monitoring is recommended.1

Use of a Examine
Control
6. 7. both the
Incubate a non- test and
processed, activated the control
Attest indicator at the indicators
same time. A control: for any color
■ ensures spore change at regular
viability intervals and, finally,
(No. 116, dry block incubator)
at 24 hours for the
■ demonstrates the 1261P, or 48 hours for
Write the Place Caution: Check the Activate
1. sterilizer 2. (test)
3. After
4. chemical (crush)
capability of the 5. the 1262P indicators.
media to promote
number, load indicator sterilization, indicator on processed Act on a positive test
growth
and date on the into a package the contents of Attest the Attest indicator indicator by inserting (yellow) immediately.
indicator. that is similar to the biological indicators for a color change into the incubator. ■ confirms that
typical packs used are hot and under from rose to brown. the incubator is
(e.g., in an autoclave pressure. Always functioning properly
bag) and in the area allow to cool. Failure
of the autoclave that
is the most difficult
to cool for at least
10 minutes may cause
Attest ™
to sterilize, i.e., over the glass ampule to Biological Monitoring System Use at Least Once
A Week Per Sterilizer
the drain or in the burst which may Sterilizer Attest Date & Time Date & Time Results Control
center of a full load. result in injury from Date
No.
& Type
Cap
Color
In Incubator/
Initials
Out Incubator/
Initials
(Circle
One)
(Circle
One)
Run cycle. flying debris.
+ +
8. Record results
in the log book. - -

Indicator Selection for Steam Autoclaves Results Interpretations

Negative Test (purple) Spores were killed. The sterilization process was successful.
Sterilization Time Packaging Attest Indicator
Gr

Gr

ity
av

it y
av

27 27
0F 0F

Positive Control (yellow)

/1 3 /1 3
2C 2C

Process (minutes) Materials (cap color)

250oF (121oC) <15 None 1262P Positive Test (yellow) Sterilization process failure. Recall all loads since last negative test. Determine
Gr

Gr

ity ity
av

av

27 27

Positive Control (yellow) cause for sterilization process failure. Reprocess load. Do not process any other
0F 0F

(steam) 20 Wrapped (Brown)

/1 3 /1 3
2C 2C

>30 Containing fabrics Biological Indicators (25) loads until biological indicators test negative in three successive cycles.
Negative Test (purple) There is a problem with spore vitality, the growth media or the incubator
270oF (132oC) >3 None 1261P
Gr

Gr

ity ity
av

av

Negative Control (purple) temperature. Check dating of the Attest indicators used. Repeat test.
27 27
0F 0F
/1 3 /1 3
2C 2C

(steam) 10 Wrapped (Blue)* If results are the same, send incubator in for servicing.
Biological Indicators (25)
Reference: 1. Miller, C. JADA, March 1992.
*The 1262P (Brown) may be substituted for the 1261P (Blue)
when monitoring the 10-minute, 270oF cycle.

3M™ Attest™
Biological Monitoring System (Item No. 116K)
Includes: one incubator, one 1262P box of 25 indicators and one log book.
3 Reliability
70-2009-2093-5