Publications in GW182, GW/P bodies, and microRNA

1. Pauley, K.M., Stewart, C.M., Gauna A.E., Dupre, L.C., Kuklani, R., Chan, A.L., Pauley,
B.A., Reeves, W.H., Chan, E.K.L., and Cha, S. (2011) Altered miR-146a expression in
Sjögren’s syndrome and its functional role in innate immunity. European J. Immunol., in press.

2. Pauley, K.M., Gauna A.E., Grichtchenko, I., Chan, E.K.L., and Cha, S. (2011) A
secretagogue-siRNA conjugate confers resistance to cytotoxicity in a cell model of Sjögren's
syndrome. Arthritis Rheum., in press.

3. Yao, B., Li. S., Jung, H.M., Lian, S.L., Abadal, G.X., Han, F., Fritzler, M.J., and Chan,
E.K.L. (2011) Divergent GW182 functional domains in the regulation of translational silencing.
Nucleic Acids Res. 39:2534-47.

4. Nahid, M.A., Rivera, M., Lucas, A., Chan, E.K.L., and Kesavalu, L. (2011) Polymicrobial
infection with periodontal pathogens specifically enhances miR-146a in ApoE-/- mice during
experimental periodontal disease. Infection Immun. 79:1597-605.

5. Nahid, M.A., Satoh, M., and Chan, E.K.L. (2011) Mechanistic role of miR-146a in
endotoxin-induced differential cross-regulation of TLR signaling. J Immunol. 186:1723-34.

6. Ceribelli, A., Tincani, A., Cavazzana, I., Franceschini, F., Cattaneo, R., Pauley, B.A.,
Chan, J.Y.F., Chan, E.K.L., and Satoh, M. (2010) Anti-argonaute2 (Ago2/Su) and -Ro
antibodies identified by immunoprecipitation in primary anti-phospholipid syndrome (PAPS).
Autoimmunity, Aug 9. [Epub ahead of print].

7. Vázquez-Del Mercado, M., Sánchez Orozco, L.V., Pauley, B.A., Chan, J.Y.F., Chan,
E.K.L., Panduro, A., Maldonado González, M., Jimenez-Luevanos, M.A., Martin Márquez, B.T.,
Palafox-Sanchez, C.A., Dávalos-Rodríguez, I.P., Salazar-Páramo, M., González Lopez, L.,
Gámez Nava, J.I., and Satoh, M. (2010) Autoantibodies to a miRNA-binding protein Argonaute
2 (Su antigen) in patients with hepatitis C virus infection. Clin. Exp. Rheumatol., Nov 25. [Epub
ahead of print].

8. Jakymiw, A., Patel, R., Deming, N., Bhattacharyya, I., Shah, P., Lamont, R.J., Stewart,
C., Cohen, D., and Chan, E.K.L. (2010) Overexpression of dicer as a result of reduced let-7
microRNA levels contributes to increased cell proliferation of oral cancer cells. Genes,
Chromosomes and Cancer 49:540-59. PMC2859695

9. Pauley, K.M., Satoh, M., Pauley, B.A., Dominguez-Gutierrez, P.R., Wallet, S.M.,
Holliday, L.S., Cha, S., Reeves, W.H., and Chan, E.K.L. (2010) Formation of GW/P bodies as
marker for microRNA-mediated regulation of innate immune signaling in THP-1 cells. Immunol.
Cell Biol. 88:205-12. PMC2824770

10. Nahid, M.A., Pauley, K.M., Satoh, M., and Chan, E.K.L. (2009) MicroRNA-146a is
critical for endotoxin-induced tolerance. Implication on innate immunity. J. Biol. Chem.
284:34590-34599. PMC2787321
11. Lian, S.L., Li. S., Abadal, G.X., Pauley, B.A., Fritzler, M.J., and Chan, E.K.L. (2009) The
C-terminal half of human Ago2 binds to multiple GW-rich regions of GW182 and requires
GW182 to mediate silencing. RNA 15:804-13. PMC2673069

12. Moser, J.J., Chan, E.K.L., and Fritzler, M.J. (2009) Optimization of immunoprecipitation-
western blot analysis in detecting GW182-associated components of GW/P Bodies. Nat Protoc.
4:674-85. NIHMS163042 PMC2797048

13. Li, S., Lian, S.L., Moser, J.J., Fritzler, M.L., Fritzler, M.J., Satoh, M., and Chan, E.K.L.
(2008) Identification of GW182 and its novel isoform TNGW1 as translational repressors in
Ago2 mediated silencing. J. Cell Sci. 121: 4134-4144.

14. Pauley, K.M., Satoh, M., Chan, A.L., Bubb, M.R., Reeves, W.H., and Chan, E.K.L.
(2008) Upregulated miR-146a expression in peripheral blood mononuclear cells from
rheumatoid arthritis patients. Arthritis Res. Ther., 10:R101.

15. Moser, J.J., Eystathioy, T., Chan, E.K.L., and Fritzler, M.J. (2007) Markers of mRNA
stabilization, degradation and RNAi within astrocytoma GW bodies. J. Neuroscience Research
85:3619-3631.

16. Bhanji, R.A., Eystathioy, T., Chan, E.K.L., Bloch, D.B., and Fritzler, M.J. (2007) Clinical
and serological features of patients with autoantibodies to GW/P Bodies. Clin. Immunol.,
125:247-256. PMC2147044

17. Lian, S., Fritzler, M.J., Katz, J., Hamazaki, T., Terada, N., Satoh, M., and Chan, E.K.L.
(2007) Small interfering RNA-mediated silencing induces target-dependent assembly of GW/P
bodies. Mol. Biol. Cell, 18:3375-87.

18. Jakymiw, A., Pauley, K.M., Li, S., Lian, S., Ikeda, K., Eystathioy, T., Satoh, M., Fritzler,
M.J., and Chan, E.K.L. (2007) The role of GW/P-bodies in RNA processing and silencing. J.
Cell Sci., 120:1317-23.

19. Fritzler, M.J., and Chan, E.K.L. (2006) GW bodies, P bodies and components of the
microRNA pathway. In Autoantibodies, 2nd edition, edited by Shoenfeld, Y., Gershwin, M.E.,
and Meroni, P.L. Elsevier Science, Chapter 34, pp257-262.

20. Chan, E.K.L. (2006) Autoantibodies and the cloning and characterization of cellular
constituents. In Autoantibodies and Autoimmunity: Molecular Mechanisms in Health and
Disease. Edited by K. Michael Pollard, Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim,
Germany, Chapter 18, pp419-446.

21. Ikeda, K., Satoh, M., Pauley, K.M., Fritzler, M.J., Reeves, W.H., and Chan, E.K.L. (2006)
Detection of the argonaute protein Ago2 and microRNAs in the RNA induced silencing complex
(RISC) using a monoclonal antibody. J. Immunol. Methods, 317:38-44.
22. Pauley, K.M., Eystathioy, T., Jakymiw, A., Hamel J.C., Fritzler, M.J., and Chan, E.K.L.
(2006) Formation of GW bodies is a consequence of miRNA genesis. EMBO Reports, 7:904-
910.

23. Jakymiw, A., Ikeda, K., Fritzler, M.J., Reeves, W.H., Satoh, M., and Chan, E.K.L. (2006)
Autoimmune targeting of key components of RNA interference. Arthritis Res. Ther., 8:R87.

24. Lian, S., Jakymiw, A., Eystathioy, T., Hamel, J.C., Fritzler, M.J., and Chan, E.K.L. (2006)
GW bodies, microRNAs and the cell cycle. Cell Cycle 5:242-5.

25. Jakymiw, A., Lian, S., Li, S., Eystathioy, T., Li, S., Satoh, M., Hamel J.C., Fritzler, M.J.,
and Chan, E.K.L. (2005) Disruption of GW bodies impairs mammalian RNA Interference.
Nature Cell Biology 7:1267-74.

26. Chan, E.K.L. (2004) Autoantibody target GW bodies - cytoplasmic foci of mRNA decay
and regulatory functions. In Autoantigens, Autoantibodies, Autoimmunity, Volume 5, Pabst
Scientific Publishers, Lengerich, Germany, p203-208.

27. Stinton, L.M., Eystathioy, T., Selak, S., Chan, E.K.L., and Fritzler, M.J. (2004)
Autoantibodies to protein transport and messenger RNA processing pathways: endosomes,
lysosomes, Golgi complex, proteasomes, assemblyosomes, exosomes and GW bodies. Clin.
Immunol. 110:30-44.

28. Yang, Z., Jakymiw, A., Wood, M.R., Eystathioy, T., Rubin, R.L., Fritzler, M.J., and Chan,
E.K.L. (2004) GW182 is critical for the stability of GW bodies expressed during the cell cycle
and cell proliferation. J. Cell Science 117:5567-78.

29. Eystathioy, T., Chan, E.K.L., Takeuchi, K., Mahler, M., Luft, L.M., Zochodne, D.W., and
Fritzler, M.J. (2003) Clinical and serological associations of autoantibodies to GW bodies and a
novel cytoplasmic autoantigen GW182. J. Mol. Med., 81:811-818.

30. Eystathioy, T., Jakymiw, A., Chan, E.K.L., Séraphin, B., Cougot, N., and Fritzler, M.J.
(2003) The GW182 protein co-localizes with mRNA degradation associated proteins hDcp1 and
hLSm4 in cytoplasmic GW bodies. RNA 9:1171-1173.

31. Eystathioy, T., Chan, E.K.L., Mahler, M., Luft, L.M., Fritzler, M.L., and Fritzler, M.J.
(2003) A panel of monoclonal antibodies to cytoplasmic GW bodies and the mRNA binding
protein GW182. Hybridoma & Hybridomics, 22:79-86.

32. Eystathioy, T., Chan, E.K.L., Tenenbaum, S.A., Keene, J.D., Griffith, K., and Fritzler,
M.J. (2002) A phosphorylated cytoplasmic autoantigen, GW182, associates with a unique
population of human mRNAs within novel cytoplasmic speckles. Mol. Biol. Cell 13: 1338-51.