Assessment of Genetic Diversity of Benguet

pine (Pinus kesiya Royle ex Gord.) from Seed

Production Areas in the Philippines Using
Microsatellite Markers

MARILYN O. QUIMADO
University Researcher II
Department of Forest Biological Sciences
College of Forestry and Natural Resources
The University of the Philippines – Los Baños
Assessment of Genetic Diversity of Benguet pine (Pinus kesiya Royle ex
Gord.) Seed Production Areas in the Philippines Using Microsatellite

Markers

Co -researchers
Dr Antonio C. Laurena
Research Professor
Crop Science Clusters
College of Agriculture
The University of the Philippines – Los Baños

Dr. Aida B. Lapis

Supervising Scientist
ERDB-DENR
Assessment of Genetic Diversity of Benguet pine (Pinus kesiya Royle ex Gord.)

Seed Production Areas in the Philippines Using Microsatellite Markers

 Significance
 Man-made activities and natural calamities threatens
the genetic diversity of P. kesiya
 Seed Production Areas (SPA) were established using
seeds from unidentified sources; no assessment of
genetic diversity
 Data management on SPA’s is very poor

 Wide genetic pool is important in establishing

plantations and implementation of conservation
program
Assessment of Genetic Diversity of Benguet pine (Pinus kesiya Royle ex Gord.)
Seed Production Areas in the Philippines Using Microsatellite Markers

Objectives

 To determine the relatedness of P. kesiya in seed

production areas at Ilocos, Benguet, Nueva Ecija,
and Bukidnon using microsatellite markers.

 To measure the diversity within- and among-SPA of

P. kesiya.
The Four SPA’s

Population Specific Location Code in the Number of

Name Analysis trees
collected
DLB Busol Watershed, Benguet 1 40

NE Minuli, Carranglan, Nueva Ecija 2 40

MB Rigid Aggregates and Mining 3 30

Corp., Carasi, Ilocos Norte
DMB Bukidnon Provincial Tree Park, 4 40
Malaybalay, Bukidnon
Highlights ( A. Relatedness of the 4 SPA’s)

 ===========================
 pop ID 1(DLB) 2(NE) 3(IN) 4(BU)
 ============================
 1 **** 0.9853 0.8968 0.8669
 2 0.0148 **** 0.8664 0.8423
 3 0.1089 0.1435 **** 0.9657
 4 0.1429 0.1717 0.0349 ****
 ============================


 * Nei's genetic identity (above diagonal) and genetic distance (below

diagonal).
 Highlight (B. Dendrogram)

+---pop1 Benguet
+---------------------------------------------1
! +---pop2 Nueva Ecija
--3
! +-----------pop3 Ilocos
+--------------------------------------2
+-----------pop4 Bukidnon
Highlight – (C. Genetic diversity)

 A. Benguet and Nueva Ecija – 47%

 B. Ilocos – 41%

 C. Bukidnon – 34%
Highlight (E. Diversity within- and among-
SPA)

Genetic diversity within SPA – 66%

Genetic diversity among SPA – 33%

In future genetic diversity study on

Benguet pine, consider collecting
more trees per site and less site
Implications for In situ Conservation

Considering the limitation in geographical

distribution of Pinus kesiya and the degree of
genetic erosion, in situ conservation is a
must.

One conservation site is within the SPA in

Benguet being a protected area
Recommendations

1. Need to assess the genetic diversity of other natural

stands of Pinus kesiya in order to determine seed
source with wider genetic base.

2. In plantation establishment, the seeds from the SPA at

Bukidnon should be mixed with seeds from Benguet
SPA to increase the genetic diversity.

3. More studies on genetic diversity assessment of

endemic, threatened and rare forest species should be
made.
Acknowledgements
 A. UPLB Basic Research Program

 B. IRRI – Dr William G. Padolina, Dr. Darshan S.

Brar, GAMMA Lab Staff

 C. IBS (Molecular Lab), IPB (Biochemistry Lab)

THANK YOU

Diwata
Mountains,
Surigao del Norte
Mindanao Island

November 2003
Photo by E.S.
Fernando
MQ-pppi-Benguet
MQ-pppi-gel 2-Benguet and NE
MOQ-ppi gel 3-NE
moq-dmb-19-35(Bukidnon)
Characteristics of the SSR Custom Primers

Primer Annealing Size of Products

Temperature (°C)

PM01 56 111-117

PM09 52 81-99

PM12 59 181-193