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Operation Manual
UP-5 pH/Temp
UP-10 pH/mV/Temp
UP-25 pH/mV/ion/Temp
°C
pH
4 7 10
setup
mode enter
standardize
902395.1 Rev. A
UltraBasic Meter Quick Reference
1. Install a 9 volt battery in the 2. Connect an electrode to the
battery compartment or con- Input and ATC connectors
nect a AC adapter to the power (optional).
connection (optional).
enter rdize
standa
setup mod
e
er et
V M
pH/m
10
UP-
°C
pH
4 7 10
setup
mode enter
standardize
Getting Started
Front panel controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2
Digital display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2
Rear panel connectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3
Battery Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3
Installing and Maintaining Electrodes . . . . . . . . . . . . . . . . . . . . . .4
Installing electrodes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4
Storing electrodes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5
Standardizing for pH Measurement . . . . . . . . . . . . . . . . . . . . . . . .6
Entering buffers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6
Using Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
Clearing Buffers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
Reviewing electrode standardization . . . . . . . . . . . . . . . . . . . . . .9
Selecting buffer sets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10
Standardizing for Millivolt Measurement
(Relative Millivolts) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11
Entering a millivolt offset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11
Using Setup in mV Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12
Clearing a millivolt offset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12
Standardizing to Ion Measurement . . . . . . . . . . . . . . . . . . . . . . .13
Entering standards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13
Using Setup in Ion Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16
Setting the values of the standard . . . . . . . . . . . . . . . . . . . . . . .17
Understanding pH Theory . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19
Defining pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19
Understanding Temperature Compensation . . . . . . . . . . . . . . . .20
U.S. Standard Buffer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20
Typical electrode response and temperature . . . . . . . . . . . . . . .20
Measuring pH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21
Measuring Ion Concentration . . . . . . . . . . . . . . . . . . . . . . . . . . . .22
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .24
Electrode testing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .24
Meter Operation testing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25
Meter Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .26
Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .26
Technical Assistance . . . . . . . . . . . . . . . . . . . . . . . . . . . Back Cover
1
Getting Started
Front Panel Controls
°C
pH
setup - press to
4 7 10
clear standards,
review electrode
calibration or
select new auto enter - press
recognized to select
buffers and stan- setup
options.
dard sets. mode enter
standardize -
mode - press to
standardize press to auto-
toggle between recognize each
pH, mV or ion buffer or stan-
mode. dard.
power - press
to turn unit on
or off
Measuring
Icon Digital Display
°C %DO
Temperature
Low Battery
Indication
mg/L Mode
Sal m S/cm K cm ion rel mV pH
Standardizing TDS µ M
Icon
Stability
Icon
S Result
standardize
enter
mode
setup
Meter
pH/mV
UP-10
Battery Installation
9V Battery
Battery
Compartment
Cover
3
Installing and Maintaining Electrodes
This meter allows you to use:
• Combination pH electrode with BNC connector
• 3-in-1 combination electrode with BNC connector and 2.5 mm
phone jack for ATC
• Separate 2.5 mm phone jack for ATC
• Ion selective or ORP electrode with BNC connector.
3. Remove the shorting cap on the BNC connector. Install the electrode by
plugging it into the input connection (push on and twist to lock). An
accessory ATC probe can be installed in the ATC port for the most accu-
rate pH measurements.
setup e
er et
V M
pH/m
10
UP-
4
4. Rinse and blot-dry electrodes 5. Store pH electrodes in KCI solu-
between each measurement (do tion or Electrode Storage
not wipe). Rinse electrodes Solution. Always leave the filling
with distilled water or deionized hole open when in use and
water, or part of the next solu- closed when in storage. Refill
tion to be measured. with Filling Solution when the
internal solution level gets 1”
below fill hole.
5
Standardizing for pH Measurement
Because electrodes vary in their response, you must standardize
your pH meter and electrode to compensate for electrode varia-
tion. The more frequently you standardize, the more accurate your
measurements. Standardize daily, or more often, for accurate
results.
NOTE: To escape standardizing mode press Standardize again
°C
pH
4 7 10
setup
mode enter
standardize
°C
pH
pH
S
Clear
4 7
2 4 7 10 12
setup setup
standardize standardize
6
5. The meter displays the percent 6. To enter a second buffer, place
slope of the electrode as 100.0% the electrode in the second
on the first buffer. On entering a buffer solution, stir, allow time
second or third buffer, the for the electrode to stabilize,
meter performs a diagnostic and press standardize again.
check on the electrode and dis- The meter recognizes the buffer.
plays the slope.
°C
pH pH
S S
OK
7 2 4 7 10 12
setup setup
standardize standardize
pH pH
OK
Error
4 7 4 7
setup setup
standardize standardize
7
9. To enter a third standard, place 10. After entering each buffer, the
the electrode in the third buffer Standardizing icon goes off and
solution, stir, allow to stabilize, the Measuring or Stable icon
and press standardize. The appears on the display to indi-
results will be the same as in cate that the meter returns to
steps 7 and 8. Measuring operation.
°C
pH pH
S
OK
7 10 4 7 10
setup setup
standardize standardize
8
Using Setup in pH Mode
The setup button lets you clear all the buffers/standards that you
have entered, review calibration information, or select the
buffer/standard set that you want.
NOTE: Continuing to press Setup will return you to measurement
mode.
1. Press setup and the meter dis- 2. Press setup again to show elec-
plays a flashing Clear icon. Use trode performance. If the meter
this step only when you wish to has accepted two buffers, it will
clear all buffers you have display Good Electrode, display
entered. To clear all existing the slope between the first and
buffers, press enter. The meter second buffers and display the
clears all buffers and returns to two buffer icons.
Measuring screen.
pH pH
OK
Clear
4 7 4 7
setup setup
standardize standardize
mode enter
standardize
9
4. Press Setup again to display a 5. Press Enter to select the set of
flashing Set Buffers icon and to buffers shown on the display
display the first buffer set (U.S. or
buffer set) icons. Press Setup again to view the
next set of buffers. Continue
pressing Setup to view the third
buffer set (NIST buffer set).
pH pH
Set Set
2 4 7 10 12 1 3 6 8 10 13
setup setup
standardize standardize
10
Standardizing for Millivolt Measurement
(Relative Millivolts)
NOTE: UP-5 does not include mV mode.
You use millivolt measurement for electrode diagnostics, titration or mea-
suring redox potential (also called ORP, oxidation reduction potential). ORP
measurements indicate the oxidizing or reducing capability of a solution.
You can use ORP values to monitor or control solutions requiring a set
amount of oxidants or reductants.
°C
mV
setup
mode enter
standardize
°C °C
mV rel mV
S S
setup setup
standardize standardize
11
Using Setup in mV Mode
1. To clear a mV offset and return 2. To clear the mV standard, press
to absolute millivolt mode, enter. You then return to
press setup. The meter displays absolute mV mode.
a flashing Clear icon, and shows
the current relative millivolt off-
set.
°C
rel mV mV
Clear
setup setup
standardize standardize
12
Standardizing for Ion Measurement
Because electrodes vary in their response, you must standardize
your meter and electrode to compensate for electrode variation. The
more frequently you standardize, the more accurate your measure-
ments. Standardize daily, or more often, for accurate results.
Note: You must first setup your standard values in setup mode.
1. Immerse electrode in standard 2. Press and release the mode but-
solution. Stir gently. Allow the ton until your digital display
electrode to reach a stable indicates ion mode.
value. Ion Selective Electrodes
may take up to 10 minutes to °C
reach a stable reading.
ion
setup
mode enter
Note: No measurement will dis-
play on screen if less than two standardize
standards are stored.
°C
ion ion
Clear
1 2 3 1
S1
setup setup
standardize standardize
°C °C
ion ion
1 1
setup setup
standardize standardize
7. The meter will flash “S2” and 8. ”S1” and “S2” are both dis-
after two seconds the 2 will played along with the change in
appear. When the signal is sta- mV/decade. Typical mV change
ble, the meter recognizes the for a monovalent ISE is 59
standard. mV/decade and 27 mV/decade
for a divalent ISE. Refer to the
ISE instructions for acceptable
mV change.
ion ion
S2 S1 S2
setup setup
standardize standardize
14
9. Slope Error indicates that the 10. To enter a third standard, place
change in mV between the stan- the electrode in the third stan-
dards displayed is too small and dard solution, stir, allow to sta-
accurate ion measurements can- bilize, and press standardize.
not be taken. Press enter to con- The results will be the same as
tinue. in steps 7 and 8.
ion ion
Error
S1 S2 S2 S3
setup setup
standardize standardize
11. After entering each standard, 12. Standardize your meter and
the Standardizing icon goes off electrode using at least two
and the Measuring or Stable standards with concentrations
icon appears on the display to bracketing the expected range
indicate that the meter returns of your samples. Stirring with a
to Measuring operation. magnetic stir bar and stirrer pro-
vides faster electrode response.
°C
ion
1 2 3
setup
mode enter
standardize
15
Using Setup in Ion Mode
The setup button lets you clear all the standards that you have
entered, review calibration information, or enter the standard val-
ues that you want.
NOTE: You can escape setup mode at any time by pressing mode.
1. Press setup and the meter dis- 2. Press setup again to show elec-
plays a flashing Clear icon. Use trode performance. If the meter
this step only when you wish to has accepted two standards, it
clear all standards you have will display OK, and display the
entered. To clear all existing mV /decade change between the
standards, press enter. The first and second standards.
meter clears all standards and
returns to Measuring screen.
ion ion
Clear
1 2 3
S1 S2
setup setup
standardize standardize
mode enter
standardize
16
4. Press setup again and “Set” and 5. The current value set for the first
“S1” will begin to flash. Press standard will be displayed (00000
enter to set the value of the first is displayed if no value is set). The
standard. first digit is flashing and is ready to
be set.
ion ion
Set Set
S1 S1
setup setup
standardize standardize
setup
mode enter
standardize
17
6. Press setup until the correct 8. Repeat steps for all digits. After
digit is displayed. the last digit, press mode until the
decimal place is in the proper loca-
7. Press the mode key to move to tion.
the second digit.
Note: To clear standard value set-
ting press mode until “clear” is
displayed and press enter.
ion ion
Set Set
S1 S1
setup setup
standardize standardize
9. Press enter to accept the 10. Press setup and repeat steps 4
value. If the value needs to be - 9 for S2 and S3.
changed, press enter key again.
ion ion
Set Set
S1 S2
setup setup
standardize standardize
18
Understanding pH Theory
Defining pH
The measurement of pH plays an important role in identifying and
controlling acidity and alkalinity levels for industry and research.
pH is a measure of the acidity or alkalinity of a solution and can be
represented by this equation:
pH = -log [H+]
Neutral
Tomato Juice
Lemon Juice
Baking Soda
Strong Base
Strong Acid
Pure water
Ammonia
Coffee
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14
pH
more acidic more basic
Figure 1.
pH Scale showing the relative acidity or basicity
of some common substances.
19
Understanding Temperature Compensation
NOTE: Temperature compensation only takes effect when an ATC
probe is attached.
electrode isopotential
potential point
(mV) 0
-500
0 7 14
ATC
Temperature
Sensor
pH Sensor
Porous
Junction
Reference
Electrode
21
Measuring Ion Concentration
The UP-25 not only has the capability of measuring pH and mV but
also measures ion concentration. In ion mode you can measure the
concentration of specific ions using an ion selective electrode (ISE).
The concept is the same as in pH mode where the activity of
hydrogen ions is measured with a pH electrode.
22
Standardizing
After setting the value of the standards (see Using Setup in Ion
Mode) it will be necessary to standardize the electrode. Place the
electrode in the lowest concentration standard to be standardized.
Typically the recommended storage solution for ion selective elec-
trodes is the lowest concentration standard regularly measured so
it should not take too much time for the electrode to stabilize. You
can press "Mode" until mV is displayed to view the stability of the
result. Follow the steps in the "Standardizing for Ion Measurement"
section to complete standardization taking care to wait for a stable
result before pressing the Standardize key.
Taking measurements
After standardization is complete, you are ready to take measure-
ments. Add ISA to your sample(s). As soon as you put the elec-
trode in the sample, measurements begin. The reading is stable
when both the "S" icon appears and when the result is no longer
changing. The meter displays three significant figures.
Errors
The most common errors in ion measurement are:
• Dilution errors
• Entering standards before the reading is stable
• Impurities in water and/or dirty glassware
• Improper care/storage of ISE
23
Troubleshooting
1. If the signal from the electrode is out 2. The meter will display Electrode
of range, the display will show “- - - Error when it detects an error in elec-
”. This may happen when the elec- trode response. During pH standard-
trode is not in a solution. ization, the message indicates that
the electrode is less than 90% or
more than 105% of the correct
response in pH mode. The Electrode
Error message can indicate either a
bad electrode or bad buffer(s).
°C
pH pH
Error
4 7
setup setup
standardize standardize
S Electrode Test
pH 7 0 ± 30 mV
pH 4 159 to 186 mV
more than pH 7
setup
24
5. To test the meter for correct operation, install the BNC (input) shorting cap. Press
mode to select the mV mode (UP-10 and UP-25 only), and note the mV reading.
Make sure the meter is in mV mode and not relative mV mode.If the meter reads
0 + 0.3 mV, it is measuring correctly. Note that a long term drift of 0.1mV/month
since last calibration is specified.
enter rdize
standa
setup mod
e
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pH/m
10
UP-
25
Accessories
You can order the following accessories for your pH meter:
Meter Specifications
pH Range 0.000 to 14.00 pH
Resolution 0.01 pH
Accuracy 0.01 pH
Standardization 1, 2 or 3 buffers
26
Warranty Instructions
1. Please return the prepaid, pre-addressed Purchase Registration Card to Denver
Instrument Company promptly upon your purchase of the Denver Instrument product.
The return of the card is not a condition precedent to warranty coverage.
2. If you have any questions about a Denver Instrument product, please contact the near-
est Denver Instrument office as listed below.
3. If it becomes necessary to return your Denver Instrument product for service, you must
obtain a “Return Authorization Number”. Please pack the product securely in its origi-
nal approved packing carton or an other suitable container. Include your Return
Authorization Number on the shipping label. Shipping charges must be fully prepaid.
Return to authorized distributor or :
North and South America: Denver Instrument Company Service Center
6542 Fig Street
Arvada, Colorado 80004
Tel: 800.321.1135
Tel: 303.431.7255
Fax: 303.423.4831
U.K. and Ireland: Denver Instrument Company
Denver House, Sovereign Way
Trafalgar Business Park
Downham Market
Norfolk PE38 9SW England
Tel: 44 136 63862 42
Fax: 44 136 63862 04
Europe, Asia and Australia: Denver Instrument GmbH
Robert-Bosch-Briete 10
37079 Gottingen Germany
Tel: 49 551 20977 31
Fax: 49 551 20977 39
Disclaimers
You have purchased a quality precision meter that requires handling with care.
Read entire contents of this Operation Manual prior to operating your new Denver
Instrument meter.
Important Notes:
IP-67 rating is valid only when AC cover is completely installed and securely in place.
The operator shall be responsible for any modifications to Denver Instrument Company
equipment and for any connections of cables or equipment not supplied by Denver
Instrument and must check and if necessary, correct these modifications and connec-
tions.
Changes or modifications not expressly approved by the manufacturer could void the
product’s warranty.
North and South America: Denver Instrument Company
1855 Blake Street, Suite 201
Denver, Colorado 80202
Tel: 800.321.1135
Tel: 303.431.7255
Fax: 303.423.4831
www.denverinstrument.com