Escolar Documentos
Profissional Documentos
Cultura Documentos
, Ambler, PA 19002
610-368-1120 (Mobile), 215-382-7705 x 2105 (O) * ying_homan@hotmail.com
Ying Homan
Summary
An experienced scientist with about eight years of biomedical research experienc
e in both academic and industrial settings. Skilled in bioassays using qPCR, FAC
S, ELISA, Western blot analysis and fluorescence cell staining; experienced with
primary cell culture and establishing immortalized cell lines from primary cell
s; expertise with compound screening using immortal cell lines and primary cell
lines. Knowledge of establishing in vivo and in vitro models for stem cell resea
rch. Expertise in molecular mechanistic studies of cellular functions in stem c
ell regeneration research.
A quick learner capable of multitasking; with extensive knowledge and skills in
modern molecular biology and cell biology; detail orientated with good organizat
ional skills; experienced in data organization and statistical analysis using Ex
cel, Statgraphics plus 5.1; experienced with pathway finding database search, su
ch as, Ingenuigy; experienced in result presentation using PowerPoint.
Professional Experience
February 2009 - present
Aderans Research Institute, Inc.
Philadelphia, PA
Senior Scientist I
* Designed and conducted biological assays to study mechanism underlying our cel
l products potencies. Screened agonists and antagonists that impacts biological
functions of the cells using GFP reporting constructs
Techniques employed include qPCR, immunofluorescence cell staining, FACS, Wester
n blot, FISH , molecular cloning to generate the reporter constructs, lipofectio
n and magnetofection, immunohistochemical staining and GFP detection by FACS ana
lysis.
* Designed and conducted bioassays to identify and validate biomarkers for regen
erative activity of human primary cells. We explored both gene array approach a
nd SILAC protein analysis approach. We also screened cell surface markers for m
arker identification.
Techniques employed include: SILAC media culture, western blotting and immunoflu
orescence staining; mRNA array analysis and qRT-PCR analysis, GUAVA, FACS and ma
gnetic cell sorting.
* Established immortalized human dermal cell lines from primary culture of human
scalp using lentiviruses to study the molecular basis of human dermal cell rege
nerative activity.
February 2008 - February 2009
Aderans Research Institute, Inc.
Philadelphia, PA
Scientist IV
* Established semi high throughput protocols to screen lineage markers of cell p
roducts for phase II clinical trial.
* Studied and screened for protein cell surface markers for lineage identificati
on of our cell products for future clinical trial and production.
* Determined the effects of BMP2, BMP4, and Noggin on the mouse dorsal skins usi
ng immunohistochemistry and fluorescence in-situ hybridization
May 2005 January 2008
Aderans Research Institute, Inc.
Philadelphia, PA
Scientist I
* Conducting bioassays of mRNA and miRNA expression of clinical trial samples us
ing quantitative RT-PCR.
* Determined the effects of SHH, FGF2 and other growth factors on the potency of
cultured human primary cells from human clinical trials
* Optimized culture conditions for primary human cells, and wrote SOPs for trans
ferring culture techniques to the manufacturing department.
* Participated in the development of animal models using nu/nu and NOD/SCID mice
for testing skin cell regenerative activities in vivo.