MUHAMAD HANAFI HAZEMI

93 Colony Manor Dr. * Rochester, New York 14623 * 585.748.6311 * mh9c2f90@westpo

st.net
QUALIFICATIONS PROFILE
======================
Detail-oriented and analytical emerging professional with four years of academic
research is prepared to leverage related expertise, education, and experience t
o contribute to the biotechnology field.
* Performed a range of molecular and biochemistry related laboratory tests, incl
uding:
~ Monoclonal antibody detection using ELISA;
~ SDS-PAGE and Western blot on transformed E.coli;
~ Performed plasmid conjugation on E.coli and bioconjugation of Myeloma and B-ly
mphocyte cells using synthetic polymer polyethylene glycol (PEG );
~ Two-color DNA microarray and qRT-PCR on yeast enzyme;
~ High Performance Liquid Chromatography (HPLC), Liquid-Liquid extraction, Solid
Phase extraction (SPE) and Gas Chromatography with Mass Spectrometry (GCMS) in
analytical chemistry;
~ DNA purification, conventional PCR and gene sequencing using Sequencher to det
ermine sequence similarity, assembly, and alignment;
~ Prepared media, suspension culture, attachment cell, microcarrier, and primary
cell cultures;
~ Conducted karyotype analysis and mycoplasma detection using PCR.
* Expertly operate, calibrate, and maintain complex and sensitive laboratory equ
ipment and instrumentation, as well as cutting-edge software programs.
* Highly motivated and goal-oriented with proven ability to thrive in challengin
g situations requiring independent decision-making.
* Demonstrated ability to work independently with minimal supervision; entrusted
with access to typically restricted areas.
* Possess excellent communication, documentation, and organazational skills.

Related Courses
============
Genomics, Immunology, Genetics, Cell Physiology, Hybridoma Technique, Tissue Cul
ture, Introduction to Microbiology, Molecular Biology, Cell Biology, Bioinformat
ics, UNIX Under the Hood, Perl for Bioinformatics, Introduction to Database and
Data Model, Computer Science I and II and Data Analysis.
Computer Skills
============
Environments: Windows, UNIX, Macintosh, Sun Solaris
Languages: Java, Perl, C++, FORTRAN, MySQL
Software: Sequencher, Magic Tool, Microsoft Office Suite, Matlab
Databases: Protein Data Bank, BLAST, NCBI
EDUCATIONAL BACKGROUND
======================
Bachelor of Science in Biotechnology, Concentration in Bioinformatics (5/2010) G
raduated with Honors
ROCHESTER INSTITUTE OF TECHNOLOGY, Rochester, NY
Deans List ~ Scholarship Recipient ~ Vice President, Malaysian Entrepreneur Club
/ Project Director, Batik Clothing Promotions ~ Career Day Volunteer ~ Sports B
ureau, Malaysian Student Association
PROFESSIONAL EXPERIENCE
=======================
ROCHESTER INSTITUTE OF TECHNOLOGY, Rochester, NY, 2006 - 2010
Biotechnology Student with Emphasis in Bioinformatics (2006 - 2010)
Completed intensive program to gain expertise in biotechnology and bioinformatic
s. Excelled in diverse coursework, including Genomics, Immunology, Genetics, Cel
l Physiology, Hybridoma Technique, Tissue Culture, Introduction to Microbiology,
Molecular Biology, Cell Biology, Bioinformatics, UNIX Under the Hood, Perl for
Bioinformatics, Introduction to Database and Data Model, Computer Science I and
II and Data Analysis.
* Conduct analysis of genetic sequencing for a range of projects to investigate
key research questions.
* Manage, manipulate, and maintain large datasets and create new libraries, incl
uding experience with major protein databases.
* Executed research and created library using large dataset of protein-protein b
inary complex interfacial interactions modeled as tetrahedral motifs from protei
ns in double centroid reduced representation (DCRR).
* Researched effectiveness of hydrocarbon-degrading bacteria in degradation of m
ixed organic waste.
Laboratory Teaching Assistant (2009)
Served as assistant for Cell Physiology labs. Oversaw student performance, ensur
ed adherence with lab protocols and safety guidelines, and responded to a range
of questions. Graded quizzes and recorded student grades. Prepared media / cell
cultures and assisted students using Magic Tool software.
* Conducted hands-on demonstrations on proper way to perform experiments.
* Provided feedback and recommendations to professors regarding various issues.