IHC Protocol Guide

R&D Systems Tools for Cell Biology Research
IHC Products & Protocol Guide
IHC & ICC cover images using R&D Systems primary antibodies.
Delta-like 1 (DLL1) in Embryonic Rat Stomach Wnt-2 in Human Stomach Cancer Tie-2 in Embryonic Zebrafish
Catalog # AF3970 Vanilloid R1 in Rat Dorsal Root Ganglion
Catalog # AF3464 Olig2 and Oligodendrocyte Marker O4 in Rat Cortical Stem Cells (RCSC)
Catalog # AF928 IL-2 Receptor a in Mouse Spleen
Catalog # AF3066 Notch-2 in Rat Choroid Plexis
Catalog # AF2418/MAB1326 STAT6 in Human Peripheral Blood Mononuclear Cells (PBMCs)
Catalog # AF2438 Glutathione Peroxidase 1 (GPX1) in Rat Liver
Catalog # AF1190 MESDC2 in Adult Mouse Testis
Catalog # MAB2167 Meprin b Subunit in Mouse Intestine
Catalog # AF3798 GFAP in Rat Astrocytes
Catalog # AF4545
Catalog # AF3300
Catalog # AF2594
To view our continuously updated IHC/ICC image gallery, please visit our website at www.RnDSystems.com/go/IHCimages
TABLE OF CONTENTS
This guide provides an introduction to the techniques, protocols, and troubleshooting methods that lead to a successful immunohistochemistry (IHC) or immunocytochemistry (ICC) experiment. Contributors include scientists from R&D Systems IHC/ICC core facility who have experience qualifying thousands of antibodies for these applications, and R&D Systems expert technical service team who have guided researchers through a range of IHC/ICC-related issues.
INTRODUCTION. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-10 Designing a Successful IHC/ICC Experiment . . . . . . . . . . . . . . . . . . . . . . . . . . 2 Sample Preparation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 Paraffin-embedded & Frozen Tissues Cell Samples Fixation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 Formaldehyde, Alcohols, & Acetone Fixation of Tissues Fixation of Cultured Cells Preventing Non-specific Staining . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Preventing Non-specific Hydrophobic Interactions Preventing Non-specific Ionic Interactions Endogenous Enzyme Interference Endogenous Biotin Interference Primary Antibody Selection & Optimization . . . . . . . . . . . . . . . . . . . . . . . . . 6 Selecting a Primary Antibody Supplier Selecting a Monoclonal or Polyclonal Antibody Affinity Purification of Polyclonal Antibodies Optimization of Primary Antibody Incubation Detection & Visualization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-8 Direct or Indirect Detection, & Amplification Fluorescence Detection Chromogenic Detection IHC/ICC Controls. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 Endogenous Tissue Background Control No Primary Antibody Control Isotype Control Absorption Control Tissue Type Control Limitations of Western Blot Comparisons Antigen Retrieval . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 Antigen Retrieval Techniques Protease-induced Epitope Retrieval (PIER) Heat-induced Epitope Retrieval (HIER) Optimization & Limitations
IHC/ICC PROTOCOLS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-20 A. Making a 4% Formaldehyde Solution in PBS . . . . . . . . . . . . . . . . . . . . 12 B. Preparation of Gelatin-coated Slides for Histological Tissue Sections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 C. Chromogenic Staining of Tissue Sections Using HRP-DAB Detection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14-15 Slide Preparation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 Chromogenic Staining Using HRP-DAB Detection . . . . . . . . . . . . . . . . 15 D. IHC Staining of Tissue Sections Using Fluorescence Microscopy . . . . . 16 E. Antigen Retrieval Using Heat-induced Epitope Retrieval (HIER) . . . . 17 F. Preparation of Coverslips & Cell Fixation for ICC . . . . . . . . . . . . . . . . . . 18 G. Fluorescent ICC Staining of Cultured Cells . . . . . . . . . . . . . . . . . . . . . . . 19 H. Preparation of a Cell Smear for Non-adherent Cell ICC . . . . . . . . . . . . . 20 TROUBLESHOOTING GUIDE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 ALPHABETICAL LISTING OF R&D SYSTEMS IHC/ICC ANTIBODIES . . . . . . . . . . . . . . . . . . . . . . . 22-108 SUPPLEMENTAL IHC/ICC REAGENTS . . . . . . . . . . . . . . . . . . . . . . . . . 109-112
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INTRODUCTION
Designing a Successful IHC/ICC Experiment
Immunohistochemistry (IHC) and immunocytochemistry (ICC) are techniques employed to localize antigen expression and are dependent on specific epitopeantibody interactions. IHC refers to the use of tissue sections, whereas ICC describes the use of cultured cells or cell suspensions. In both methods, positive staining is visualized using a molecular label, which can be fluorescent or chromogenic. Briefly, samples are fixed to preserve cellular integrity, and then subjected to incubation with blocking reagents to prevent non-specific binding of the antibodies. Samples are subsequently incubated with primary and secondary antibodies, and the signal is visualized for microscopic analysis. Technically, IHC/ICC are relatively simple and straightforward experimental methods. However, there are many variables which must be recognized and optimized for each individual IHC/ICC study. The most challenging aspect of these techniques is determining the experimental conditions necessary to generate a strong and specific signal for each antigen of interest. For example, detection of an abundant protein in cultured cells may require a short fixation period, minimal blocking, and may be compatible with direct visualization using a flurochrome-conjugated primary antibody. In contrast, detection of a phosphorylation-dependent epitope in a section of frozen tissue may require antigen retrieval and be dependent on amplified chromogenic visualization. This guide provides information on the IHC/ICC variables listed below and is intended to assist in the design, optimization, and troubleshooting of IHC/ICC experiments.
VARIABLES FOR EXPERIMENTAL DESIGN & OPTIMIZATION

VARIABLE Antigen Epitope Sample Type Sample Preparation Appropriate Controls Fixation Method Fixative* Blocking Reagent* Antigen Retrieval* Detection Method Primary Antibody* Secondary Antibody* Labeling Method Label Counterstain* Mounting Reagent Visualization & Analysis FACTORS Species, expression levels, sample types, & subcellular location Dependence on conformation or post-translation modification Tissue or cells Tissue: embedded or frozen Cells: adhered cell cultures or cell suspensions No primary antibody, isotype control, absorption control, tissue type control Perfusion or immersion (with or without freezing) Formaldehyde, alcohols, or acetone Normal serum, bovine serum albumin (BSA), or non-fat milk Protease-induced Epitope Retrieval (PIER) or Heat-induced Epitope Retrieval (HIER) Direct or indirect (with or without amplification) Monoclonal or polyclonal Species and label Fluorescence or chromogenic Fluorochromes and spectral properties Chromogens: 3,3' Diaminobenzidine (DAB), 3-amino-9-ethylcarbazole (AEC) Fluorescence: 4,6'-diamidino-2-phenylindole (DAPI) Chromogenic: hematoxylin Fluorescence: anti-fade mounting medium Chromogenic: aqueous mounting medium Fluorescence microscope or standard light microscope
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Examples of Fluorescence Detection by IHC & ICC. A. Fluorescence detection by IHC was used to visualize V-set and Ig domain-containing protein 1 (VSIG1) in a frozen section of human testis using anti-human VSIG1 affinity-purified polyclonal antibody (Catalog # AF4818). Tissue was stained using NorthernLights-557 anti-sheep IgG secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue). B. Fluorescence detection was used to measure Glial Fibrillary Acidic Protein (GFAP) expression in differentiating cultures of rat cortical stem cells using anti-human GFAP affinity-purified polyclonal antibody (Catalog # AF2594). The cells were stained using anti-sheep NorthernLights-557 secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue).
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* These variables require optimization of additional factors including concentration, pH, temperature, incubation time, and diluent. This table is not intended to be comprehensive but represents the most commonly applied IHC/ICC methods at R&D Systems.
Examples of Chromogenic Detection by IHC & ICC. A. Chromogenic detection of Interferon Regulator Transcription Factor 8 (IRF8) expression in paraffin-embedded human lymphoma tissue section using anti-human IRF8 affinity-purified polyclonal antibody (Catalog # AF5117). The tissue was stained using the anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue). B. Chromogenic detection of IFN-g expression in human peripheral blood mononuclear cells, stimulated with calcium ionomycin (0.5 mg/mL) and PMA (50 ng/mL), using anti-human IFN-g affinity-purified polyclonal antibody (Catalog # AF285). The cells were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
For research use only. Not for use in diagnostic procedures.
INTRODUCTION
Sample Preparation
Tissue and cell samples must be appropriately harvested and prepared for each IHC/ICC study. To facilitate the required incubation steps, whole tissues must be cut into ultra thin (5-10 mm) sections or cut into smaller pieces for whole mount IHC. For ICC experiments, cells must be attached to a microscope slide or coverslip before commencing the staining procedure. Sample preparation is also intimately linked to the method of fixation, which in turn is influenced by the desired detection technique (fluorescence versus chromogenic). In most circumstances, one experimental variable will determine the most appropriate method of sample preparation. For example, tissue that is immersion-fixed in formaldehyde must be paraffin-embedded and cut using a microtome. Alternatively, to detect a phosphorylationdependent epitope, tissue may need to be snap frozen, which requires a cryostat for sample sectioning followed by alcohol fixation.
Paraffin-embedded Tissue
Paraffin embedding offers the best option for long-term preservation of tissue samples. Tissue must be fixed before being embedded in paraffin. Fixation can be achieved by perfusion or immersion immediately following dissection, and typically requires 4-24 hours. It is not recommended to fix tissue for more than 24 hours because overfixation can cause masking of the antigen (see page 10). If necessary, tissues can be transferred to alcohol after fixation until there is time for the embedding process. Because paraffin is immiscible with water, tissue must be dehydrated before adding molten paraffin wax. Dehydration is achieved by immersion in increasing concentrations of alcohol. This approach allows for a gradual change in hydrophobicity and minimizes cell damage. Following dehydration, the tissue is incubated with xylene to clear any remaining ethanol. Paraffin is typically heated to 60 C for embedding and is subsequently allowed to harden overnight. The tissue is subsequently cut with a sharp blade into ultra thin slices using a microtome. Sections are then dried onto microscope slides and can be stored at room temperature for extended periods of time. Tissue sections must be rehydrated before commencing the IHC/ICC protocol (see page 14). Paraffin is the cheapest and most commonly used substance for tissue embedding. However, tissue can also be embedded in plastic, which sets harder and allows thinner tissue sections (1.5 mm versus 5 mm).
IHC SAMPLE PREPARATION COMPARISON

TISSUE Paraffin-embedded FIXATION SECTIONING STORAGE ADVANTAGES LIMITATIONS Pre-embedding Microtome Multiple years at room temperature Preserves tissue morphology Overfixation can mask the epitope Frozen Post-sectioning Cryostat 1 year at - 80 C Preserves enzyme and antigen function Formation of ice crystals may negatively affect tissue structure
Cell Samples
To perform ICC studies, the cells must first be attached to a solid support, such as a microscope slide or coverslip. This is simple with adherent cells, which can be grown directly on high-grade, sterilized coverslips placed on the bottom of a 6- or 24-well plate. Cell adhesion can be enhanced by precoating the coverslip with a charged polymer like Poly-L-Lysine and/or an extracellular matrix protein such as Laminin, Fibronectin, or Collagen. All steps of the staining process can be performed with the coverslip in the microplate by adding the appropriate solution to each well and removing solutions by aspiration. Cells in suspension can be adhered to a slide by Cytospin centrifugation or by a 10 minute incubation of the cells on slides that have been coated with Poly-L-Lysine. Note: It is important to be able to recognize artifacts of poorly prepared samples in order to properly interpret results. For example, folds or tears in the tissue, or bubbles between the tissue/cells and the slide, can collect staining reagents non-specifically and obscure positive staining or lead to false-positive signals.
Frozen Tissue
One of the benefits of frozen tissue samples is the time saved by omitting the initial fixation step required for paraffin-embedded tissue. Snap freezing can be particularly beneficial when detecting post-translational modifications such as phosphorylation. Frozen tissue can be prepared by immersing the tissue in liquid nitrogen or isopentane, or by burying it in dry ice. For frozen samples, a short fixation is done after freezing and sectioning. Frozen tissue sections are most often fixed using alcohol, which avoids the possible requirement to retrieve epitopes masked by formaldehyde cross-linking. Frozen tissue is cut on a cryostat and sections can be stored at - 80 C for up to 1 year. Frozen tissue sections can be processed in a shorter amount of time than paraffin-embedded sections. However, freezing is not adequate for longterm preservation of the tissues and the formation of ice crystals within the cells may negatively affect subcellular detail. In addition, frozen sections are often thicker than paraffin sections. This can increase the potential for lower microscopic resolution and poor images of tissue morphology. Because frozen tissue often retains enzymatic activities, blocking the functions of endogenous enzymes that may affect the IHC detection method is also particularly important (see page 5).
Cytospin is a registered trademark of Thermo Electron Corporation.
INTRODUCTION
Fixation
All samples used in IHC/ICC experiments must be fixed to preserve tissue morphology and retain the antigenicity of the target molecules. Fixation alters the chemical composition of tissues and often requires a compromise between preserving tissue structure and preserving the epitope. Incomplete xation (underxation) of cells or tissues may allow rapid proteolytic degradation of target proteins within the tissue and can reduce specific immunoreactivity. However, excessive xation (overfixation) may result in masking of the epitope or in strong non-specic background staining that can obscure specic labeling. As discussed in the previous section, the method and timing of fixation must be considered in terms of sample preparation. In addition, duration, temperature, and pH will all affect the degree of fixation.
Formaldehyde
Formaldehyde is the most common xative used to preserve protein targets within tissues and cells. Formaldehyde-mediated tissue fixation is thought to be dependent on the formation of protein-protein and proteinnucleic acid cross-links involving methylene bridges (-CH2-). Formaldehyde can chemically link NH2 (amino) and CONH (peptide) groups, NH2 and NH, or NH2 and NH2 groups. Formaldehyde is a good choice for most IHC/ICC applications but is not a universal fixative. Overfixation with formaldehyde can modify the amino acids that are part of an epitope and can block an antibody from binding to it. However, in most cases the epitope can be unmasked to restore antibody binding using antigen retrieval techniques (see page 10). Formaldehyde has been shown to induce intracellular translocation of phosphorylation-dependent epitopes from the membrane to the cytoplasm. In situations like this, ice cold absolute methanol or absolute ethanol are appropriate alternative fixatives. Note: Although there are many different xatives available, formaldehyde is the most commonly used, and is an appropriate starting point for most IHC/ICC experiments. Formaldehyde-based solutions should be aliquoted and frozen, or stored at 4-8 C for no longer than one month. See page 12 for a protocol for making a typical 4% formaldehyde solution.
Acetone
Acetone is a strong dehydrating agent, which can cause irreversible precipitation of tissue proteins. It is usually used on sections of unxed, snapfrozen tissues, followed by xation with alcohols or formaldehyde.
Fixation of Tissues
Whole animal perfusion fixation is often the best way to preserve antigens when studying intact tissues of small animals such as mice, rats, and guinea pigs. It involves replacing the animals systemic blood with a fixative solution. However, whole animal perfusion may not be sufficient to fix the tissues of interest. In cases such as these, dissected tissues can be immersed in the fixative. For example, 4% formaldehyde in PBS is a commonly used solution for perfusion and immersion fixation of tissues. To enhance penetration of the fixative during immersion fixation, it is recommended that tissues be no thicker than 10 mm. For complete fixation, the volume of the fixative should be 50-100 times larger than the volume of the tissue. Fixation is usually achieved at room temperature for 4-24 hours. It is very important to optimize fixation conditions since underfixation or overfixation may reduce or abolish tissue immunoreactivity.
Fixation of Cultured Cells

In contrast to tissue samples, cultured cells can be fixed for a shorter period of time with a less concentrated fixative solution. For example, fixation with a 2% formaldehyde solution for 20 minutes at room temperature is often sufficient to preserve both cellular morphology and antigenicity. Fixation of cultured cells is usually achieved by simply discarding the culture medium and adding the fixative solution. However, the change in surface tension following removal of the culture medium can damage some cell types. If this is the case, the fixative can be added directly into the culture medium. For example, adding the same volume of 4% formaldehyde as the volume of culture medium will result in a 2% formaldehyde solution, which is strong enough to pre-fix the cells. After 2 minutes, the pre-fixation culture medium should be replaced with a fresh volume of 2% fixative. The pre-fixation step makes the cells more rigid so they can withstand any potential deleterious effects created by changes in surface tension. Note: Fixation can result in hydrophobic cross-linking of tissue proteins. The time, temperature, pH, and fixative used will determine the degree of cross-linking. Once the fixation protocol has been optimized, the same procedure should be used consistently.
Alcohols
The most popular alcohols used for cell and tissue fixation are methanol and ethanol. The molecular structures of methanol and ethanol closely resemble that of water. Therefore, they compete with water for protein hydrogen bonds, replacing water molecules in tissues. This causes proteins to precipitate at their isoelectric points by reducing their dielectric constant, and can block antibody-epitope binding due to changes in conformation. While alcohols affect the tertiary structure of proteins by breaking hydrophobic bonds, it appears that they can stabilize the secondary structure of proteins. However, it is generally thought that alcohols do not preserve tissue morphology as well as formaldehyde-based fixatives. Alcohols do not penetrate as well as formaldehyde and are primarily used to fix frozen tissue sections and cells. Consequently, alcohol fixation is more suitable for membrane surface antigens. Antigen retrieval is not recommended following alcohol fixation as it is generally considered too harsh and will likely compromise the integrity of the tissue sections or cells.
INTRODUCTION
Preventing Non-specific Staining
Once the tissue or cell samples have been appropriately prepared and fixed, the samples are ready to be stained. All IHC/ICC studies are dependent on specific antibody-epitope binding, which is governed by hydrophobic interactions, ionic interactions, hydrogen bonding, and other intermolecular forces. However, the same attractive forces can also result in non-specific staining, i.e. binding of the primary antibody to amino acids other than those within the desired epitope of the antigen. This is a common problem that occurs in IHC/ICC experiments. The challenge is to reduce non-specific interactions without impairing antibody-epitope binding. Causes of non-specific staining include interactions of the primary and secondary antibodies with serum proteins, ionic interactions between antibodies and tissues, and interactions with endogenous molecules capable of affecting the IHC detection system used. These issues can result in high background and an inability to visualize the antigen of interest in its appropriate cellular location. Staining problems of this type can be addressed by blocking non-specific interactions using a blocking reagent. These steps are performed prior to incubation of the sample with the primary antibody.
Preventing Non-specific Hydrophobic Interactions

Although hydrophobic interactions play an essential role in epitopeantibody binding, these forces can also promote non-specific binding. Most proteins have some degree of hydrophobicity due to the neutral side chains of several amino acids. Tissue incubation with heat-inactivated normal serum or bovine serum albumin (BSA) is a common procedure used to reduce non-specific hydrophobic binding. Selection of the type of normal serum is important to prevent interactions with the primary or secondary antibodies, or with the tissues/cells being stained. For example, goat serum would not be advised as a blocking reagent for use with a goat-derived primary antibody. Instead, a serum identical to the host animal of the secondary antibody or from an unrelated species is recommended. BSA and non-fat dry milk are also frequently used as blocking reagents. One of these reagents is typically included in the diluents for the primary and secondary antibodies. The addition of non-ionic detergents including 0.3% Triton X-100 or Tween 20 can also reduce nonspecific hydrophobic interactions. negatively impact staining specificity. Due to their single epitope specificity, increasing ionic strength is more likely to impair monoclonal than polyclonal antibody performance.
Endogenous Enzyme Interference

Chromogenic detection methods often use a conjugated enzyme to visualize epitope-antibody interactions. When using this method of detection, the endogenous activity of the same enzyme must be blocked. For example, protocols that include horseradish peroxidase (HRP) or alkaline phosphatase (AP) may require reagents to prevent non-specific signals. Tissues such as kidney, liver, or vascular areas with red blood cells, contain endogenous peroxidase activity. Peroxidase blocking reagents formulated with 3-10% H2O2 can be used to prevent endogenous peroxidase from cleaving the substrate. Endogenous AP found in intestine, kidney, lymphoid and other tissue can be blocked with 1 mM Levamisole. The intestinal form of AP is unaffected by Levamisole but can be blocked by using 1% acetic acid.
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Blocking Non-specific Binding with Serum. A. CD14 was detected in paraffin-embedded human tonsil tissue using anti-human CD14 biotinylated affinity-purified polyclonal antibody (Catalog # BAF383). Tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013). Tissue was stained using high sensitivity streptavidin conjugated to HRP (HSS-HRP) and DAB, and counterstained with hematoxylin (blue). B. Non-specific background staining is markedly reduced in a parallel experiment which included a blocking step using animal serum for 15 minutes at room temperature prior to incubation with the primary antibody. HSS-HRP and animal blocking serums are included in all R&D Systems Cell and Tissue Staining Kits (see page 112 for available kits).
Quenching Endogenous Peroxidase Activity. A. Failing to quench endogenous peroxidase prior to staining produced a false positive signal in sections of human kidney. Tissue was stained using the anti-goat HRP-DAB Cell & Tissue Staining Kit (Catalog # CTS008; brown). B. Endogenous peroxidase activity was quenched in the same tissue using 3% H2O2 in methanol for 15 minutes at room temperature prior to staining.
Endogenous Biotin Interference

Streptavidin binding to a biotinylated primary or secondary antibody is another common detection system that is utilized for IHC/ICC experiments (see page 7). Therefore endogenous biotin must be blocked before streptavidin incubation. Endogenous biotin is found in many tissues including liver, kidney, heart, brain, and lung. Pre-incubation of the sample with avidin is routinely used to block endogenous biotin. This must be followed by a subsequent incubation with biotin to block additional biotin binding sites on the avidin molecule. Following these biotin/avidin blocking steps, the sample can be incubated with the primary antibody.
Preventing Non-specific Ionic Interactions

If the antibody employed and the target tissue have net opposite charge, ionic interactions can cause non-specific background staining. For example, non-specific staining may be due to attraction between opposing carboxyl and amino groups. Van der Waals forces, weak electrostatic interactions between dipolar molecules, can also contribute. Increasing the ionic strength of the fixative and/or the antibody diluent buffer can reduce ionic interactions. However, epitope-antibody binding is commonly dependent on ionic forces, so this approach also has the potential to
Triton X-100 is a trademark of Dow Chemical Corporation. Tween is a trademark of ICI Americas.
INTRODUCTION
Primary Antibody Selection & Optimization
The most important factor when designing an IHC/ICC experiment is selection of the primary antibody. In turn, the critical feature of a primary antibody is specificity for the epitope. All steps of an IHC/ICC experiment must be optimized to visualize specific staining and minimize non-specific background signals. This includes performing initial studies to determine the appropriate incubation conditions for each primary antibody. The working dilution for an antigen afnity-puried polyclonal antibody is generally lower than that of a monoclonal antibody but these values must be determined empirically. To achieve a robust and specific signal, a high quality antibody that exhibits minimal cross-reactivity should be employed.
Selecting a Primary Antibody Supplier

When searching for a primary antibody, it is likely that products will be available from several different commercial sources that may offer varying degrees of antibody quality. This initial choice can make the difference between a successful experiment and a missed opportunity. A first step is to look for independent verification. This includes checking for prior successful use of the antibody in the literature. Many commercial suppliers, including R&D Systems, can provide assistance to researchers looking for this information. Another potential question to ask is whether the antibody is actually manufactured by the supplier or is it purchased and resold? Purchasing direct ensures the commercial source has had complete control of manufacturing, quality control processes, and shipping and storage conditions. In addition, the manufacturer may be in the best position to provide quality technical service should questions arise about the use of the antibody. reduce background staining, and produce more consistent results compared to non-purified antibodies. Monoclonal Antibody Binding Polyclonal Antibody Binding
Monoclonal and Polyclonal Antibody Binding. Monoclonal antibodies bind only a single epitope, while polyclonal antibodies bind different epitopes on the same protein,
Optimization of Primary Antibody Incubation

Primary antibody concentration, diluent, incubation time, and temperature all impact the quality of staining. These variables need to be optimized for each antibody and sample to achieve specific staining and low background. Often optimization is approached by maintaining a constant incubation time and temperature, while varying the antibody concentration to determine when an optimal signal is achieved with low background noise. For example, if a high affinity antibody is used, then the antibody can be used at a relatively high concentration for a shorter incubation time. Alternatively, a high affinity antibody at lower antibody concentration may be used with a longer incubation time. Longer incubation durations are often employed to ensure penetration throughout tissue sections used for stereological techniques. To promote specific staining, longer incubation periods are often conducted at lower temperatures (i.e. 4 C versus room temperature). Optimization of R&D Systems staining protocols for tissue sections typically begin with an overnight incubation with the primary antibody at 4 C. For staining cells, a 1 hour incubation with the primary antibody at room temperature is a common starting point. The working dilution of an antigen affinity purified polyclonal antibody is generally lower (from 1.7-15 mg/mL) than that of a monoclonal antibody (5-25 mg/mL). When comparing samples stained with different concentrations of the same antibody, it is important to use a consistent time and temperature for the incubation step. When working with a new antibody for the first time, it is recommended to conduct preliminary studies testing a broad range of antibody concentrations.
Selecting a Monoclonal or Polyclonal Antibody

The inherent features of monoclonal and polyclonal antibodies are associated with advantages and limitations when applied to IHC/ICC. Monoclonal antibodies, produced from a single B cell clone, represent a homogeneous population that bind with high affinity and specificity to a single epitope. This can be particularly useful when detecting a single member of a protein family that shares a high percentage of amino acid identity. Antibody binding is often dependent on the target protein maintaining its native conformational state. Access to a desired epitope can be compromised by interactions with other proteins, post-translational modifications, temperature, pH, fixation, and salt concentration. Because polyclonal antibodies are heterogeneous and can recognize multiple epitopes, they are less likely to be affected by changes in protein conformation. In general, polyclonal antibodies are also more stable than monoclonal antibodies over a range of pH and salt concentration. For these reasons, polyclonal antibodies are more frequently used for IHC/ICC experiments than monoclonal antibodies.
Affinity Purification of Polyclonal Antibodies

Polyclonal antiserum contains a mixture of antibodies produced by a large number of B cell clones. Antibodies comprising polyclonal antiserum bind to target epitopes with different specicities and afnities, as well as crossreact with irrelevant target molecules (non-specic interactions). To enrich for antibodies with the highest specicity and afnity toward the antigen of interest, R&D Systems polyclonal antibodies are antigen affinity purified. During this process polyclonal antiserum is passed through an affinity column containing immobilized antigen molecules. Specic antibodies are retained by the immobilized antigen, whereas non-specic antibodies pass through the column and are discarded. The retained antigen affinity purified antibodies are subsequently eluted from the column. Antigen afnity puried antibodies interact predominantly with their target antigen,
STARTING CONDITIONS FOR PRIMARY ANTIBODY INCUBATION

SAMPLE Tissue Cells Advantage Limitation ANTIBODY Monoclonal 5-25 mg/mL, overnight at 4 C 5-25 mg/mL, 1 hour at room temperature Single epitope specificity Vulnerable to epitope masking Polyclonal 1.7-15 mg/mL, overnight at 4 C 1.7-15 mg/mL, 1 hour at room temperature Lower concentration required Heterogeneous population
INTRODUCTION
Detection & Visualization
Following incubation with the primary antibody, antibody binding is visualized using an appropriate detection system. The method of detection can be direct or indirect, and may generate a fluorescent or chromogenic signal. Direct detection involves the use of primary antibodies that are directly conjugated to a label. Indirect detection methods utilize a labeled secondary antibody raised against the primary antibody host species. Indirect methods can also include amplification steps to increase signal intensity. Commonly used labels for the visualization of epitope-antibody interactions include fluorophores and enzymes that convert soluble substrates into insoluble, chromogenic end products. The choice of label is affected by the detection method employed, personal preference, and the type microscope equipment that is available
Direct or Indirect Detection, & Amplification

The choice of whether to use direct or indirect detection is often dictated by the level of antigen expression. For example, detection of a highly expressed epitope might be possible using a primary antibody directly conjugated to a label. Advantages of direct detection include the ease of use for multicolor staining and the elimination of concerns regarding nonspecific binding of the secondary antibody. The major disadvantage is that direct detection may lack the sensitivity required to visualize lower expression levels. In rare cases the conjugation process may also have a negative impact on the affinity of the primary antibody. In contrast, indirect detection methods generally have a higher level of sensitivity and generate a more intense signal. The signal is amplified using the indirect method because of the potential for at least two labeled secondary antibodies to bind to each primary antibody. However, the use of a secondary antibody requires extra blocking steps and additional controls. Further amplification of the signal can be achieved by taking advantage of the strong affinity of avidin and streptavidin to bind biotin. Avidin is a glycoprotein in egg white that combines stoichiometrically with biotin. Streptavidin is purified from the bacterium Streptomyces avidinii, is not glycosylated, and exhibits lower non-specific binding than avidin. Both proteins bind four biotins per molecule. If a biotinylated secondary antibody is employed, the signal can be significantly amplified by subsequent incubation with an avidin-biotin complex (ABC Method), or labeled streptavidin-biotin (LSAB Method). Streptavidin may be conjugated to a detection enzyme (i.e. horseradish peroxidase or alkaline phosphatase) or fluorochrome. Using these amplification methods requires additional steps to prevent non-specific binding (see page 5).
DETECTION METHODS & SIGNAL INTENSITY

DIRECT
ADVANTAGE Good for multicolor labeling INDIRECT
LIMITATION Lower sensitivity
ADVANTAGE Higher sensitivity
LIMITATION Potential non-specific binding of secondary antibodies AMPLIFICATION ABC METHOD
ADVANTAGE Increased signal intensity
LIMITATION Requires additional time & controls
KEY:
Antigen Primary Antibody Secondary Antibody Label Avidin/Streptavidin Biotin ADVANTAGE Increased signal intensity LIMITATION Requires additional time & controls AMPLIFICATION LSAB METHOD
INTRODUCTION
Fluorescence Detection
Fluorescence detection is based on the use of fluorochromes that emit light when excited by light of a shorter wavelength. The fluorochrome can be conjugated directly to the primary or secondary antibody, or to streptavidin. Immunofluorescence is commonly used for the simultaneous visualization of multiple cellular targets. For example, tissues can be incubated with a mixture of different primary antibodies followed by incubation with secondary reagents conjugated to fluorochromes that emit light at different wavelengths. Multicolor experiments must be designed to limit cross-reactivity between the detection reagents and crossover between the spectral properties of the fluorochromes being used. To limit cross-reactivity between secondary reagents, the primary antibodies should be derived from different species. This allows for the use of species-specific secondary antibodies that recognize only one primary antibody. However, there may be some instances when it is necessary to simultaneously use antibodies from the same species. This can be achieved using a biotinylated form of one of the primary antibodies. When using this technique, tissues must be incubated with the non-biotinylated antibody first, followed by incubation with the fluorophore-conjugated secondary antibody. The tissues are then incubated with the biotinylated primary antibody followed by incubation with a streptavidin-conjugated fluorochrome. This approach will ensure that the streptavidin conjugate will only bind the biotinylated antibody, limiting cross-reactivity.
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Emission Intensity
400
450
500
550
600
650
700
Wavelength (nm)
B.
Detection
Emission Filter Dichroic Mirror Excitation Light Source TYPICAL FLUORESCENCE FILTER SET Excitation Filter Sample
R&D Systems NorthernLights Fluorochromes have Non-overlapping Spectral Properties that make them Ideal for Multicolor IHC and ICC. A. Emission curves for NorthernLights-493 anti-goat IgG (Catalog # NL003; green) and NorthernLights-557 anti-mouse IgG (Catalog # NL007; red). B. Multicolor detection of Nestin and b-III Tubulin in rat cortical stem cells (Catalog # NSC001) using anti-rat Nestin affinity-purified polyclonal antibody (Catalog # AF2736; green) and anti-neuron specific b-III Tubulin monoclonal (clone TuJ-1) antibody (Catalog # MAB1195; red). Cells were stained with NorthernLights-493 (Catalog # NL003; green) and NorthernLights-557 (Catalog # NL007; red) secondary antibodies and counterstained with DAPI (blue).
Chromogenic Detection
To facilitate chromogenic detection, the primary antibody, secondary antibody, or streptavidin is conjugated to an enzyme. When a soluble organic substrate is applied, the enzyme reacts with the substrate to generate an insoluble colored product that is localized to the sites of antigen expression. Commonly used enzymes include horseradish peroxidase (HRP) and alkaline phosphatase, which convert 3,3' diaminobenzidine (DAB) and 3-amino-9-ethylcarbazole (AEC), into brown and red end products, respectively. DAB is more commonly used than AEC since the latter is soluble in alcohol and is more prone to fade when exposed to excessive light. Aqueous counterstain and mounting media must be used with AEC. Chromogenic detection is considered to be more sensitive than that of immunofluorescence, but is less convenient because it includes more incubation and blocking steps. Like immunofluorescence, chromogenic detection allows for the visualization of multiple antigens, but only if the antigens are confined to different locations in the cell and tissue because overlapping colors may obscure results. An advantage of DAB chromogenic staining is that the colored precipitate formed during the reaction between HRP and DAB is not sensitive to light and the slides can be stored for many years. Unlike fluorescence microscopy, which requires a specialized light source and filter sets, chromogenic techniques require only a typical light microscope.
Multicolor Fluorescence Microscopy. A typical immunofluorescence setup that depicts the light source, a suitable filter set for the fluorochrome of interest, and a detection mechanism.
NorthernLights is a trademark of R&D Systems.
INTRODUCTION
IHC/ICC Controls
Appropriate controls are critical for the accurate interpretation of IHC/ICC results. A satisfactory IHC/ICC experimental design produces results that demonstrate that the antigen is localized to the correct specialized tissues, cell types, or subcellular location. Optimization of fixation, blocking, antibody incubation, and antigen retrieval steps will generate a strong and specific signal. However, IHC/ICC experiments must include positive and negative controls to support the validity of staining and identify experimental artifacts. In addition, variances in antibody specificity, experimental conditions, biological conditions between tissue types, and even researchers may generate inconsistent staining and lead to inaccurate conclusions. To facilitate consistent performance, detailed record keeping is an important factor for IHC/ICC studies. Here, we describe several established controls that can be used to support the specificity of your IHC/ICC results.
Endogenous Tissue Background Control

Certain cells and tissues may have inherent biological properties resulting in background staining that could lead to a misinterpretation of the results. Before applying primary antibodies, cells and tissues should be inspected under the microscope using either uorescence (for fluorescent labels) or bright-field (for chromogenic labels) illumination to ensure there is no signal inherent to the tissue itself. For instance, lipofuscin is an endogenous autofluorescent pigment that can be confused with positive staining. The antigen to antibody mixture should be made at a working dilution of 10:1 (molar ratio) and be pre-incubated overnight at 4 C. The pre-absorbed antibody can then be incubated with tissue in place of the primary antibody alone. The staining pattern produced by the primary antibody can be compared to that produced by the pre-absorbed antibody. Absorption controls work better if the immunogens are peptides. However, if antibodies were raised against the whole protein, addition of the mixture of antibody plus protein may result in higher non-specific staining. Although the mechanism is unclear, the antigen used for pre-absorption may itself bind to the tissue and result in non-specific staining. Thus, it is important to note that an absorption control using whole protein may not always confirm the specificity of an antibody for the protein in the tissue.
A.
B.
C.
A.
B.
Lipofuscin Background in Nervous System Tissue. Lipofuscin is a pigment that accumulates with age in many tissue types. It also has autofluorescent properties that overlap with the excitation and emission spectra of commonly used fluorochromes. Circled in the micrographs above are lipofuscin-containing neurons that may appear labeled using either bright-field microscopy (A) or fluorescence microscopy in the green (B) and red spectrums (C).
No Primary Antibody Control

A control in which the tissue is incubated with antibody diluent, without the primary antibody included, is always necessary. This is followed by incubation with secondary antibodies and detection reagents. Staining with detection reagents alone should be negligible to the point that it does not obscure specic staining or resemble the specific staining pattern.
Absorption Control in Rat Dorsal Root Ganglion. A. A cryostat section of rat dorsal root ganglion stained for phospho-MSK1 (S212) using anti-human Phospho-MSK1 (S212) affinity purified polyclonal antibody (Catalog # AF1036). B. Nuclear staining (indicated by arrows) is abolished if the antibody is first pre-absorbed with the S212 phosphorylated immunogen.
Tissue Type Control

Additional controls for IHC/ICC experiments include using tissue samples that are known to express (or not express) the epitope of interest. This strategy can provide a useful reference and may also be utilized during initial optimization studies. Tissue from transgenic animals that overexpress or do not express the antigen can be particularly useful. In addition, tissue samples from different species can be included to support the speciesspecificity of an antibody.
Isotype Control
This control can be utilized when working with monoclonal primary antibodies. The sample is incubated with antibody diluent, supplemented with a non-immune immunoglobulin of the same isotype (for example, IgG1, IgG2A, IgG2B, IgM) and concentration as the primary monoclonal antibody. The sample is then incubated with the secondary antibody and detection reagents. These steps will help ensure that what appears to be specic staining was not caused by non-specic interactions of immunoglobulin molecules with the sample. Background staining should be negligible and not resemble specic staining.
Limitations of Western Blot Comparisons

Western blot experiments are often conducted to complement and support IHC/ICC studies. However, changes in protein conformation during denaturation can result in potentially misleading results. For a given antibody, inconsistent results between Western blot and IHC/ICC studies may simply reflect differences in the experimental conditions employed. Since multiple epitopes are recognized, polyclonal antibodies are less vulnerable to such experimental artifacts.
Absorption Control
To demonstrate that an antibody is binding specifically to the antigen of interest, it is first pre-incubated with the immunogen. This should inactivate the antibody and the tissue should show little or no staining.
INTRODUCTION
Antigen Retrieval
Although fixation is essential for the preservation of tissue morphology, this process can also have a negative impact on IHC/ICC detection. Fixation can alter protein biochemistry such that the epitope of interest is masked and can no longer bind to the primary antibody. Masking of the epitope can be caused by cross-linking of amino acids within the epitope, cross-linking unrelated peptides at or near an epitope, altering the conformation of an epitope, or altering the electrostatic charge of the antigen. Antigen retrieval refers to any technique in which the masking of an epitope is reversed and epitope-antibody binding is restored.
Antigen Retrieval Techniques

The need for antigen retrieval depends on multiple variables, including but not limited to, the target antigen, the antibody used, the type of tissue, and the method and duration of fixation. Because they recognize multiple epitopes, polyclonal antibodies are more likely than monoclonal antibodies to detect a given antigen without the use of antigen retrieval techniques. There are multiple techniques to restore the immunoreactivity of an epitope. Methods as simple as changing the pH or the cation concentration of the antibody diluent can influence the affinity of an antibody for its epitope. For partially masked epitopes it may be appropriate to first try increasing the primary antibody incubation conditions before commencing antigen retrieval. However, this step will also require further optimization in terms of appropriate antibody concentration, incubation time, and temperature. When discussing antigen retrieval methods, techniques generally fall into two main categories, protease-induced epitope retrieval (PIER) and heat-induced epitope retrieval (HIER). Once optimized, the effects of antigen retrieval can be pronounced. artifactual staining should always be considered when using any antigen retrieval methodology. The use of controls to demonstrate specific antibody binding should be included since staining is influenced by multiple variables in any given experiment.
TIME 1 minute 5 minutes 10 minutes ANTIGEN RETRIEVAL SOLUTION pH Acidic Slide #1 Slide #4 Slide #7 Neutral Slide #2 Slide #5 Slide #8 Basic Slide #3 Slide #6 Slide #9
Optimization of HIER. A typical experimental set-up to determine optimum HIER incubation time and pH. Results should be compared to a tenth slide, with no HIER. A similar approach can be employed to optimize incubation temperature.
Protease-induced Epitope Retrieval (PIER)

In the PIER method, enzymes including Proteinase K, Trypsin, and Pepsin have been used successfully to restore the binding of an antibody to its epitope. The mechanism of action is thought to be the cleavage of peptides that may be masking the epitope. The disadvantages of PIER are the low success rate for restoring immunoreactivity and the potential for destroying both tissue morphology and the antigen of interest.
R&D Systems offers reagents to improve tissue antigen detection and enhance immunoreactivity. During initial optimization, investigators can compare samples treated with neutral pH 7.0 Universal Antigen Retrieval Solution (Catalog # CTS015) with a slide of the same tissue without antigen retrieval. Subsequent tests using acidic or basic antigen retrieval solutions may be necessary depending on the tissue. At R&D Systems, we find that treatment with Basic Antigen Retrieval reagent (Catalog # CTS013) is frequently successful. We also offer Acidic (Catalog # CTS014) and Sampler (Catalog # CTS016) Antigen Retrieval Reagents. Acidic and basic antigen retrieval solutions are more likely to affect tissue morphology than a neutral solution.
Heat-induced Epitope Retrieval (HIER)

HIER is believed to reverse some cross-links and allows for restoration of secondary or tertiary structure of the epitope. The protocol must be optimized for each tissue, fixation method, and antigen to be studied. In general, HIER has a much higher success rate than PIER. HIER is performed using microwave ovens, pressure cookers, vegetable steamers, autoclaves, or water baths. Microwaves are an increasingly popular appliance for HIER. These protocols tend to involve 5 minute periods of heat followed by replacement of the buffer. For all HIER methods, slides must be cooled before commencing IHC/ICC incubations. HIER is especially time-, temperature-, buffer-, and pH-sensitive, and the best method must be determined empirically.
No HIER
Acidic HIER
Neutral HIER
Basic HIER
Optimization & Limitations

Antigen retrieval may require increased adherence of the sample to the slide or coverslip. In addition, the technique is often too harsh for cryostat tissue sections and alcohol-fixed tissue. The time, temperature, and pH must also be optimized for each appliance (i.e. 5-10 minutes at 92-95 C in a water bath, versus 1-5 minutes at 120 C in a pressure cooker). To optimize antigen retrieval preliminary studies must be conducted using a matrix of time, temperature, and pH combinations. The possibility of
Antigen Retrieval Improves Detection of p27. IHC images show the detection of p27 in paraffinembedded human prostate cancer sections following incubation of tissue for 10 minutes at 95 C in the specified antigen retrieval solution. Compared to no HIER treatment, p27 detection was enhanced following incubation in neutral (pH 7.0) and basic (pH 9.5) (Catalog # CTS013) but not acidic (pH 5.0) (Catalog # CTS014) antigen retrieval solution. p27 was detected using antihuman/mouse/rat p27 monoclonal antibody (Catalog # MAB22561; brown).
10
IHC/ICC PROTOCOLS
IHC/ICC PROTOCOLS
Immunohistochemistry Immunocytochemistry
BLOCK PRIMARY ANTIBODIES SECONDARY ANTIBODIES LABEL COUNTERSTAIN COVERSLIP
RAGE in Alzheimers Brain.
Meteorin in Rat Cortical Stem Cells.
11
IHC/ICC PROTOCOLS
A. Making a 4% Formaldehyde Solution in PBS The vast majority of IHC/ICC procedures employ fixation of tissues and cells using formaldehyde-based fixatives.
The protocol below describes the technique for generating a 4% formaldehyde solution in PBS. The most effective fixative must be determined experimentally. Caution: Formaldehyde is toxic, read the MSDS before working with this chemical. Gloves and safety glasses should be worn, and solutions made inside a fume hood.
Please read the protocol in its entirety before starting.
SUPPLIES REQUIRED
REAGENTS Deionized H2O HCl (Dilute) NaOH (1 N) Paraformaldehyde powder PBS (1x): 0.137 M NaCl, 0.05 M NaH2PO4 , pH 7.4 MATERIALS Filter units Glassware and stir bar (Dedicated for formaldehyde solution) Gloves and eye protection Hot plate with magnetic stirrer Thermometer Ventilated hood
40 g Paraformaldehyde Powder 1 N NaOH UNTIL POWDER DISSOLVES
800 mL 1x PBS AT 60 C
HEAT WHILE STIRRING
PROCEDURE
1. For 1 L of 4% Formaldehyde add 800 mL of 1x PBS to a glass beaker on a stir plate in a ventilated hood. Heat while stirring to approximately 60 C. Take care that the solution does not boil. 2. Add 40 g of paraformaldehyde powder to the heated PBS solution. 3. The powder will not immediately dissolve into solution. Slowly raise the pH by adding 1 N NaOH dropwise from a pipet until the solution clears. 4. Once the paraformaldehyde is dissolved the solution should be cooled and filtered. 5. Adjust the volume of the solution to 1 L with 1x PBS. 6. Recheck the pH and adjust it with small amounts of dilute HCl to approximately 6.9. 7. The solution can be aliquoted and frozen, or stored at 4 C for no longer than one month.
COOL & FILTER WITH FILTER PAPER ADJUST THE VOLUME TO 1 L WITH 1x PBS
ADJUST THE pH TO 6.9 WITH DILUTE HCl ALIQUOT & FREEZE
H C H
Formaldehyde
H2O
H C H
OH OH
Methylene Glycol
Formaldehyde Structure. In an aqueous solution, formaldehyde exists primarily as methylene glycol.
The difference between paraformaldehyde, formaldehyde, & formalin. Paraformaldehyde (chemical name is polyoxymethylene) is a powder of polymerized formaldehyde that by itself cannot x tissues. To be usable as a tissue xative, paraformaldehyde has to be dissolved in hot water to become a formaldehyde solution. Formalin is a saturated formaldehyde solution in water (37% by weight, 40% by volume) containing 10-15% methanol. Methanol is added to slow down the polymerization of formaldehyde, which reduces the xing power of formalin. Formalin can also be made in an alcohol-free form from powdered paraformaldehyde.
12
IHC/ICC PROTOCOLS
B. Preparation of Gelatin-coated Slides for Histological Tissue Sections In order for tissue sections to be retained on histological slides during staining
and washing steps, slides need to be coated with adhesive compounds. Although there are a variety of such compounds, gelatin is the most frequently used for histological purposes.
SUPPLIES REQUIRED
REAGENTS Gelatin-coating solution: 1 L deionized H2O, 5 g gelatin, 0.5 g chromium potassium sulfate CrK(SO4)2 MATERIALS Filter units Histological slides Hot plate with magnetic stirrer Slide racks Staining dish Thermometer
5 g Gelatin AFTER THE GELATIN DISSOLVES ADD 0.5 g CrK(SO4)2
1 L Deionized H20
HEAT AT 45 C WHILE STIRRING
PROCEDURE
1. Prepare the gelatin-coating solution by dissolving 5 g of gelatin in 1 L of heated, deionized H2O (temperature should not exceed 45 C). 2. After the gelatin has dissolved, add 0.5 g of chromium potassium sulfate. Chromium potassium sulfate will positively charge the slides allowing them to attract negatively charged tissue sections. 3. Filter this solution and store at 2-8 C until use. It is recommended that this solution be filtered again immediately before use (adjust to room temperature before filtration). 4. Place the histological slides into metal racks. Note: The slides should be cleaned by washing them in soapy water and rinsing them thoroughly, first in tap water and finally in deionized water. 5. Dip the racks containing the slides 3 to 5 times (~5 seconds each) into the gelatin-coating solution. 6. Remove the racks containing the slides and let them drain. Blot excess solution from the racks onto filter paper (gently tap the racks against the filter paper for better drainage). 7. Place the racks containing the slides on the lab bench and cover them with paper towels to protect them from dust. 8. Dry at room temperature for 48 hours. 9. Dried slides can be put back into the boxes that they arrived in and stored at room temperature until use. Slides intended for cryostat sections can be stored at -20 C.
COOL & FILTER AT ROOM TEMPERATURE
ADD Gelatin-coating Solution
DIP RACKS WITH SLIDES 3 to 5 TIMES, 5 SECONDS EACH
Staining Dish
Histological Slides
DRY AT ROOM TEMPERATURE FOR 48 HOURS
STORE AT ROOM TEMPERATURE OR AT -20 C IF INTENDED FOR CRYOSTAT SECTIONS
13
IHC/ICC PROTOCOLS
C. Chromogenic Staining of Tissue Sections Using HRP-DAB Detection The following protocol has been developed and optimized by R&D Systems IHC/ICC laboratory using the HRP-DAB Cell and Tissue Staining Kits (see page 112). Protocols may need to be modified depending on the type of tissue used. Each investigator should determine the optimal working dilution of an antibody. If using R&D Systems primary antibodies, refer to the product data sheets to obtain approximate working dilutions. For all other reagents follow the manufacturers instructions.
Caution: DAB is hazardous. Gloves and safety glasses should be worn, and all steps performed inside a fume hood.
SUPPLIES REQUIRED
REAGENTS Aqueous Mounting Medium (Catalog # CTS011, or equivalent) Primary Antibodies Cell and Tissue Staining Kit: (See page 112) Anti-goat (Catalog # CTS008), Anti-mouse (Catalog # CTS002), Anti-rabbit (Catalog # CTS005), Anti-rat (Catalog # CTS017) Kit contents: Peroxidase Blocking Reagent, Avidin Blocking Reagent, Biotin Blocking Reagent, Biotinylated Secondary Antibodies, High Sensitivity Streptavidin-HRP Conjugate (HSS-HRP), DAB Chromogen Solution, Serum Blocking Reagents Deionized H20 Dilution buffer: PBS (1x), 1% bovine serum albumin, 1% normal donkey serum, 0.3% Triton X-100, and 0.01% sodium azide PBS (1x): 0.137 M NaCl, 0.05 M NaH2PO4 , pH 7.4 Wash buffer: PBS (1x) For Paraffin-embedded tissue sections : Xylenes (mixed isomers) 100% denatured alcohol 95% denatured alcohol 70% denatured alcohol 50% denatured alcohol OPTIONAL REAGENTS Antigen retrieval reagents (see page 17) DAB Enhancer (Catalog # CTS010) MATERIALS Coverslips Hydrophobic barrier pen Slides with paraffin-embedded or cryostat tissue sections
If necessary, perform antigen retrieval (see page 17 for protocol). If endogenous peroxidase is present in the tissue 3. Incubate the sample with 1-3 drops peroxidase blocking reagent for 5 minutes Note: This procedure may affect the morphology of unfixed frozen tissue sections. Reduction in the incubation time may be required. 4. Rinse the sample, then gently wash in wash buffer for 5 minutes. 5. Surround the tissue with a hydrophobic barrier using a barrier pen. Proceed to the staining protocol on page 15.
CRYOSTAT SECTIONS THAW SLIDES AT ROOM TEMPERATURE 10-20 MINUTES

Xylene
PARAFFIN-EMBEDDED TISSUE SECTIONS

100% Alcohol 95% Alcohol 70% Alcohol 50% Alcohol
2 times 2 times 1 time 10 minutes 10 minutes 5 minutes
1 time 5 minutes
1 time 5 minutes
PROCEDURE
1. Slide preparation for Cryostat Sections a. When staining cryostat sections stored in a freezer, thaw the slides at room temperature for 10-20 minutes. Slide preparation for Paraffin-embedded Tissue Sections a. Immerse the slides in xylene (mixed isomers) 2 times for 10 minutes each. b. Immerse the slides in 100% alcohol 2 times for 10 minutes each. c. Immerse the slides in 95% alcohol for 5 minutes. d. Immerse the slides in 70% alcohol for 5 minutes. e. Immerse the slides in 50% alcohol for 5 minutes. f. Rinse the slides with deionized H20. 2. Rehydrate the slides with wash buffer for 10 minutes. Drain the excess wash buffer. If proceeding directly to one of the staining protocols on pages 15 or 16, surround the tissue with a hydrophobic barrier using a barrier pen.
REHYDRATE THE SLIDES WITH Wash Buer FOR 5 MINUTES
RINSE WITH Deionized H20
DRAIN EXCESS Wash Buer AND PERFORM ANTIGEN RETRIEVAL IF NECESSARY
14
IHC/ICC PROTOCOLS
Chromogenic Staining Using HRP-DAB Detection
PROCEDURE
1. Block the slides with 1-3 drops of serum blocking reagent for 15 minutes. Drain the slides and wipe away any excess blocking reagent before proceeding to the next step. Do not rinse. 2. Incubate the sample with 1-3 drops of avidin blocking reagent for 15 minutes. Rinse the sample with wash buffer, drain slides, and wipe away any excess wash buffer. 3. Incubate the sample with 1-3 drops of biotin blocking reagent for 15 minutes. Rinse with wash buffer, drain the slides, and wipe away any excess wash buffer. 4. Incubate the sample with primary antibodies in dilution buffer. Follow manufacturers recommendations regarding working dilution, incubation time, and temperature. These variables may need to be modified for your system. 5. Rinse the sample with wash buffer and then wash 3 times in wash buffer for 5 minutes each and drain the slides. 6. Incubate the sample with 1-3 drops of biotinylated secondary antibodies for 30-60 minutes, adjusting the incubation time depending on the thickness of the section (approximately 30 minutes for 5-10 mm thick sections and 60 minutes for 10-20 mm thick sections). 7. Rinse the sample with wash buffer then wash 3 times in wash buffer for 15 minutes each and drain the slides. 8. Incubate the sample with 1-3 drops of HSS-HRP for 30 minutes. 9. Rinse the sample with wash buffer then wash 3 times in wash buffer for 2 minutes each. 10. Calculate the working volume of DAB Chromogen Solution given that 100-200 mL is required to cover the entire tissue section on a slide. Add 1-5 drops of DAB Chromogen Solution to cover the entire tissue section and incubate for 3-20 minutes. Monitor the intensity of the tissue staining under a microscope. Note: DAB Enhancer can be used to intensify the DAB Chromogen solution. DAB is hazardous. Refer to the MSDS for safe deactivation. 11. Rinse the sample with wash buffer 3 times for 10 minutes each. 12. Rinse in deionized H20 and drain the slides. 13. Stained tissue can be mounted either without counterstaining or counterstained with hematoxylin for better visualization of the tissue morphology. 14. Cover stained tissue with a coverslip of an appropriate size. Place slides vertically on a filter paper or towel to drain excess mounting medium and allow them to dry. 15. Visualize tissues under a light microscope.
BLOCKING (FOLLOW STEPS 1-3)
RINSE WITH Wash Buer, DRAIN SLIDES ADD Primary Antibodies INCUBATE THE SAMPLE ACCORDING TO THE MANUFACTURERS RECOMMENDATIONS RINSE 3 TIMES FOR 5 MINUTES EACH WITH Wash Buer, AND DRAIN ADD Biotinylated Secondary Antibodies
INCUBATE FOR 30-60 MINUTES
RINSE 3 TIMES FOR 15 MINUTES EACH WITH Wash Buer, AND DRAIN ADD HSS-HRP
INCUBATE FOR 30 MINUTES
RINSE AND WASH 3 TIMES IN Wash Buer FOR 2 MINUTES EACH ADD DAB Chromogen Solution
INCUBATE 3-20 MINUTES MONITORING INTENSITY OF TISSUE STAINING UNDER A MICROSCOPE RINSE 3 TIMES FOR 10 MINUTES EACH WITH Wash Buer & 1 TIME WITH Deionized H2O
COUNTERSTAIN IF REQUIRED
MOUNT WITH Aqueous Mounting Medium
15
IHC/ICC PROTOCOLS
D. IHC Staining of Tissue Sections for Fluorescence Microscopy The following protocol has been developed and optimized by R&D Systems IHC/ICC laboratory
using the NorthernLights fluorescent secondary reagents (see page 110). Protocols may need to be modified depending on the type of tissue used. Each investigator should determine the optimal working dilution of an antibody. If using R&D Systems primary antibodies, refer to the product data sheets to obtain approximate working dilutions. For all other reagents follow the manufacturers instructions. Caution: DAB is hazardous. Gloves and safety glasses should be worn, and all steps performed inside a fume hood.
ADDITIONAL SUPPLIES FOR FLUORESCENT IHC STAINING

REAGENTS Primary Antibodies Anti-fade Mounting Medium (i-BRITE Plus, Catalog # SF40000, Neuromics, Inc., or equivalent) Blocking buffer: 10% normal donkey serum, 0.3% Triton X-100 Dilution buffer: PBS (1x), 1% bovine serum albumin, 1% normal donkey serum, 0.3% Triton X-100, and 0.01% sodium azide NorthernLights Fluorescent Secondary Antibodies/Streptavidin Conjugates (or equivalent) PBS (1x): 0.137 M NaCl, 0.05 M NaH2PO4 , pH 7.4 Wash buffer: PBS (1x) MATERIALS Coverslips Slides with paraffin-embedded or cryostat tissue sections
IHC Staining Using Fluorescence Microscopy 5. Incubate for 1 hour at room temperature with a NorthernLightsconjugated secondary antibody (or equivalent) in dilution buffer designed to recognize the host species of the primary antibody. Alternatively, a NorthernLights-streptavidin conjugate (or equivalent) can be used if the primary antibody is biotinylated. Note: Some tissues may bind non-specifically to biotin-avidin labeling components resulting in high background staining. (See page 5) 6. Wash the slides 3 times for 15 minutes each in wash buffer. 7. Mount with an anti-fade mounting medium.
BLOCK THE SLIDE WITH Blocking Buer (STEP 2)
PROCEDURE
1. Slide preparation (See Step 1 on page 14) 2. Block the slides using blocking buffer for 15 minutes at room temperature and rinse with wash buffer. 3. Make working dilutions of the primary antibodies in dilution buffer according to manufacturers instructions. Add small volumes of these dilutions to the slides and incubate at 4 C overnight, or for 30-60 minutes at room temperature. Note: Multiple primary antibodies to different antigens can be added in step 5 as long as care is taken to avoid the use of secondary reagents with overlapping spectra. If possible, primary antibodies should also be raised in different species. Please see page 8 for more details. Note: A negative control and/or an isotype-matched control should be included to identify non-specific binding from the secondary antibody. Please see page 5 for more details about IHC/ICC controls. 4. Wash the slides 3 times for 15 minutes each in wash buffer.
INCUBATE FOR 15 MINUTES AT ROOM TEMPERATURE
RINSE WITH Wash Buer ADD Primary Antibodies INCUBATE SAMPLE AT 4 C OVERNIGHT OR 1 HOUR AT ROOM TEMPERATURE RINSE 3 TIMES IN Wash Buer FOR 15 MINUTES ADD Secondary Antibodies INCUBATE FOR 1 HOUR AT ROOM TEMPERATURE
RINSE 3 TIMES IN Wash Buer FOR 15 MINUTES MOUNT WITH Anti-fade Medium
Triton X-100 is a trademark of Dow Chemical Corporation.
16
IHC/ICC PROTOCOLS
E. Antigen Retrieval Using Heat-induced Epitope Retrieval (HIER) Antigen retrieval can reveal epitopes masked during the preparation of tissues for staining. The protocol below describes a technique used by R&D Systems and can be used on both cryostat and paraffin-embedded sections. The protocol can be used with R&D Systems Acidic, Basic, or Universal Antigen Retrieval Reagents. Pretreatment with these solutions may induce a dramatic enhancement of immunoreactivity. However, they also have the potential to affect tissue morphology. The optimal method of antigen retrieval must be determined experimentally. For examples of IHC studies using antigen retrieval, see page 10.
SUPPLIES REQUIRED
REAGENTS Antigen Retrieval Solution (10x): Antigen Retrieval Reagent-Basic (Catalog # CTS013) or Antigen Retrieval Reagent-Acidic (Catalog # CTS014) or Antigen Retrieval Reagent-Universal (Catalog # CTS015) Deionized H2O PBS (1x): 0.137 M NaCl, 0.05 M NaH2PO4 , pH 7.4 EQUIPMENT Polypropylene Coplin staining jar (or equivalent) Water bath at 92-95 C MATERIALS Slides with paraffin-embedded or cryostat tissue sections HEAT SLIDES IN Antigen Retrieval Solution FOR 2-10 MINUTES 92-95 C
Water bath ALLOW TO COOL AT ROOM TEMPERATURE RINSE WITH Deionized H20 AND THEN WITH PBS AVOID VIGOROUS RINSING
PROCEDURE
1. Make working dilutions by mixing 1 part of 10x Antigen Retrieval concentrate with 9 parts of deionized water. 2. Preheat retrieval solution to 92-95 C. This may be achieved by placing a polypropylene Coplin staining jar filled with retrieval solution into a water bath. Note: Heating may cause cracking of glass staining dishes. 3. Immerse slides into preheated retrieval solution for 2-10 minutes. Note: Since the effect of antigen retrieval reagents depends on their temperature (90-100 C) and incubation time (up to 30 minutes), optimal conditions should be determined by the individual investigator. Note: Cryostat sections are more sensitive to damage by retrieval solution than paraffin-embedded tissues. To avoid tissue damage, it may be necessary to shorten the incubation time to 2-5 minutes. 4. After the incubation is finished, remove the Coplin jar with retrieval solution and slides from the water bath, and let it cool to room temperature. 5. Gently rinse the slides with deionized water and then with PBS. Note: Because tissues may be loosened after the retrieval procedure, avoid vigorous rinsing to prevent detachment from the slides. 6. Tissues are now ready for blocking and primary antibody incubation.
Coplin Staining Jar
BEGIN STAINING PROCEDURE
17
IHC/ICC PROTOCOLS
F. Preparation of Coverslips & Cell Fixation for ICC The fundamental principles of IHC experiments using tissue sections also apply to ICC experiments. However,
these protocols must be specially adapted for use with cultured cells or cell suspensions. The following protocols describe how to prepare gelatin-coated coverslips and fix cultured cells for ICC experiments.
Please read the protocols in its entirety before starting.
Coverslip Preparation Using Gelatin Many cultured cell types do not adhere well to glass coverslips. There are several techniques used to enhance cell adhesion including the use of polyamino acids such as Polyornithine or Poly-L-Lysine, and/or extracellular matrix proteins such as Laminin or Fibronectin. Adding a thin layer of gelatin to a coverslip is also often used to enhance the adhesion of cultured cells to glass. SUPPLIES REQUIRED
REAGENTS Gelatin-coating solution: 0.1% gelatin in deionized H2O MATERIALS Coverslips (Sterilized) Cell culture plate (6- or 24-well)
Preparation & Fixation of Cells on Coverslips SUPPLIES REQUIRED

REAGENTS Fixative: 2-4% formaldehyde in PBS Wash buffer: 0.1% BSA in 1x PBS Cell culture medium PBS (1x): 0.137 M NaCl, 0.05 M NaH2PO4, pH 7.4
MATERIALS Gelatin-coated coverslips in a 24-well plate
PROCEDURE
1. Culture cells by adding 0.5 mL of culture media containing approximately 5,000 cells to the wells of a cell culture plate containing gelatin-coated coverslips. 2. When cells have reached the desired density/age, remove the culture media from each well and wash twice with PBS. 3. Add 300-400 mL of 2-4% formaldehyde to each well and incubate for 20 minutes at room temperature. 4. Wash the wells twice with PBS and then cover with 400 mL of wash buffer. The coverslips can be stored at 2-8 C for up to 3 months or they may be stained immediately. Note: Fixation can result in hydrophobic cross-linking of tissue proteins. The time, temperature, pH, and fixative used will determine the degree of cross-linking. Once the fixation protocol has been optimized, the same procedure should be used consistently.
PROCEDURE
1. Sterilize coverslips and place them into the wells of a 24-well plate. 2. Add 400 mL of gelatin-coating solution and incubate the coverslips for 10 minutes at room temperature. 3. Remove the gelatin-coating solution and air dry the coverslips for 15 minutes. 4. The dried coverslips can now be stored at room temperature until use.
PLATE CELLS ON GELATIN-COATED COVERSLIPS
CULTURE TO DESIRED DENSITY/AGE
ADD 4% Formaldehyde FOR 20 MINUTES AT ROOM TEMPERATURE
ADD 400 L of Wash Buer STAIN OR STORE AT 2-8 C FOR UP TO 3 MONTHS
WASH 2 TIMES WITH PBS
WASH 2 TIMES WITH PBS
18
IHC/ICC PROTOCOLS
G. Fluorescent ICC Staining of Cultured Cells The following protocol has been developed and optimized using R&D Systems primary antibodies and NorthernLights fluorescent secondary antibodies (see page 8). This protocol may need to be optimized for each specific ICC experiment.
SUPPLIES REQUIRED
REAGENTS Primary Antibodies Blocking buffer: 10% normal donkey serum, 0.3% Triton X-100 DAPI (4',6-diamidino-2-phenylindole) solution: add 1 mL of 14.3 mM stock for every 5 mL of PBS. Store any unused DAPI at 2-8 C, wrapped in aluminum foil Deionized H2O Dilution buffer: PBS (1x), 1% bovine serum albumin, 1% normal donkey serum, 0.3% Triton X-100, and 0.01% sodium azide Anti-fade mounting medium NorthernLights-conjugated secondary reagents (see page 110), or equivalent PBS (1x): 0.137 M NaCl, 0.05 M NaH2PO4, pH 7.4 Wash and antibody dilution buffer: 0.1% BSA in PBS (1x) MATERIALS Cell-covered coverslips in a 6- or 24-well plate Fine tweezers
10. Carefully remove the coverslips from the wells and blot to remove any excess water. Dispense 1 drop of Anti-fade mounting medium onto the microscope slide per coverslip. Mount the coverslip with the cells facing towards the slide. 11. Visualize using a fluorescence microscope and filter sets appropriate for the label used. Slides can also be stored in a slide box at < -20 C for later examination.
BLOCK
RINSE FIXED CELLS WITH Wash Buer INCUBATE SAMPLE 45 MINUTES AT ROOM TEMPERATURE ADD Primary Antibodies NO RINSING IS NECESSARY INCUBATE OVERNIGHT AT 2-8 C OR FOR 1 HOUR AT ROOM TEMPERATURE ADD Secondary Antibodies RINSE 2 TIMES WITH Wash Buer
PROCEDURE
Note: This protocol is optimized for cells grown on coverslips in a 6- or 24-well plate but can be adapted accordingly. 1. Wash the coverslips or slides containing the fixed cells two times in 400 mL of wash buffer 2. Add 400 mL of blocking buffer and block for 45 minutes at room temperature. 3. Remove blocking buffer. No rinsing is necessary. 4. Dilute the unconjugated primary antibody (or fluorescenceconjugated primary) in dilution buffer according to the manufacturers instructions. Incubate overnight at 2-8 C, or alternatively, incubate at room temperature for 1 hour. Note: Experimentation with both antibody concentration and incubation time may be required to achieve optimal labeling. 5. Wash two times in 400 mL of wash buffer. If using a primary antibody with a direct fluorescent conjugate, go to step 8. 6. Dilute the secondary antibody in dilution buffer according to the manufacturers instructions. Add 400 mL to the wells and incubate at room temperature for 1 hour in the dark. From this step forward samples should be protected from light. 7. Wash two times in 400 mL of wash buffer. 8. Add 300 ml of the diluted DAPI solution to each well and incubate 2-5 minutes at room temperature. DAPI binds to DNA and is a convenient nuclear counterstain. It has an absorption maximum at 358 nm and fluoresces blue at an emission maximum of 461 nm. 9. Wash once with PBS and once with water.
INCUBATE FOR 1 HOUR ADD DAPI Counterstain
RINSE 2 TIMES WITH Wash Buer
INCUBATE 2-5 MINUTES AT ROOM TEMPERATURE RINSE 1 TIME WITH PBS RINSE 1 TIME WITH Deionized H2O REMOVE THE COVERSLIP WITH A FINE TWEEZERS MOUNT WITH THE CELLS FACING THE SLIDE
19
IHC/ICC PROTOCOLS
H. Preparation of a Cell Smear for Non-adherent Cell ICC One method of preparing non-adherent cells for ICC involves smearing the sample across a gelatincoated slide. This technique forms a monolayer of cells that can be easily visualized.
SUPPLIES REQUIRED
REAGENTS Deionized H2O Fixative: 4% formaldehyde in PBS (1x) PBS (1x): 0.137 M NaCl, 0.05 M NaH2PO4, pH 7.4 MATERIALS Microfuge tube (1.5 mL) Gelatin-coated microscope slides Hot plate Hydrophobic barrier pen Microfuge FIX CELLS FOR 20 MINUTES AT ROOM TEMPERATURE SPIN DOWN CELLS FOR 30 SECONDS RESUSPEND IN 1 mL Deionized H2O
PROCEDURE
1. Fix the cells with 4% formaldehyde for 20 minutes at room temperature by adding formaldehyde directly to the culture media (see page 4) and adjust to approximately 1 x 10 6 cells/mL. 2. Add 1 mL of the cell solution to a 1.5 mL microfuge tube. 3. Spin down the cells for 30 seconds in a microfuge. 4. Pour off the supernatant and resuspend the cell pellet in 1 mL of deionized H2O. 5. Spin down the cells for 30 seconds in the microfuge. 6. Pour off the supernatant and resuspend the cell pellet in 200 mL of deionized H2O. 7. Add 5 mL of the cell suspension to a gelatin-coated slide (3 spots per slide) and smear with the side of a pipette tip. 8. Place the slide on a hot plate (low heat setting) and allow the liquid to evaporate. 9. Check the slide under a microscope and make sure that there are no salt crystals. If salt crystals are observed, wash the slide with water. 10. Surround the cell spot with a hydrophobic barrier using a barrier pen and air dry. 11. The slides can be stored at 2-8 C for up to 3 months or they may be stained immediately.
SPIN DOWN CELLS FOR 30 SECONDS
RESUSPEND IN 200 L Deionized H2O
ADD 5 L Cell Suspension TO GELATIN-COATED SLIDE
SMEAR WITH A PIPETTE TIP
HEAT TO EVAPORATE THE LIQUID
SURROUND CELLS WITH A Hydrophobic Barrier
20
TROUBLESHOOTING GUIDE
Problem: Lack of Staining
Possible Source
Lack of antigen. Antibodies do not work due to improper storage. Inactive primary or secondary antibodies. Inadequate tissue xation. Tissue overxation. Incompatible secondary and primary antibodies. Antigen was destroyed before incubation with the primary antibody. Epitope altered during fixation or embedding procedure. Antigen retrieval was ineffective. Reagents omitted or used in wrong order.
Test or Action
Check protein expression by in situ hybridization (in some rare cases translation may be blocked even though mRNA is detected). Follow storage instructions on the datasheet. In general, aliquot antibodies into smaller volumes sufficient to make a working solution for a single experiment. Store aliquots in a manual defrost freezer (-20 to -70 C) and avoid repeated freeze-thaw cycles. Test reporter system independently to assess reagent viability. Try increasing the fixation time or try a different xative. Reduce the duration of the immersion or post-xation steps. If immersion xation cannot be avoided (for example, collection of postmortem tissues or biopsies in pathology lab), antigens may be unmasked by treatment with antigen retrieval reagents. Use a secondary antibody that will interact with the primary antibody. For example, if the primary antibody was raised in rabbits, use an anti-rabbit secondary antibody. If quenching of endogenous peroxidase was done prior to the addition of primary antibodies, block peroxidase after incubation with the primary antibody. Try restoring immunoreactivity through various antigen retrieval techniques. Embed tissue at 58 C or below. Increase the time of treatment or change the treatment solution. Repeat staining and confirm that correct reagents are used and are added in the correct order.
Problem: High Background

Possible Source
High concentration of primary and or secondary antibodies. Hydrophobic interactions of the antibody and proteins in the tissue. Non-specic binding of primary and/or secondary reagents to tissues. Non-specific binding of secondary antibody. Tissue dried out. Reagents sticking to old or poorly prepared slides. Background due to ionic interactions.
Test or Action
Titer antibody to determine optimal concentration needed to promote a specific reaction of the primary and the secondary antibodies. Lower the ionic strength of the antibody diluent (particularly monoclonal antibodies respond well to reducing the salt concentration). Use blocking step just prior to primary antibody incubation (we use 1% bovine serum albumin with 10% normal donkey serum, or non-fat dry milk is another option). Use an antibody that has cross-reactive IgG species removed (absorbed against sample species). Avoid letting the tissue dry during the staining procedure. Start over with freshly prepared or purchased slides. Increase the ionic strength of the diluent buffer.
Problem: Cell/Tissue Morphology Destroyed

Possible Source
Antigen retrieval methods are too harsh. Tissue sections falling off slide. (more common with frozen sections) Tissue section appears torn or folded. Air bubbles under section. Poor resolution of tissue morphology Underfixation has physically damaged the tissue or cells during the staining process Autolysis of tissue leading to staining of necrotic debris.
Test or Action
Empirically determine the conditions that preserve tissue morphology while restoring the immunoreactivity of the antigen. Increase fixation time. Empirically determine an additional or alternative fixative. Use freshly prepared, adequately charged slides. Re-cut sections using a sharp blade, or ignore damaged areas when analyzing the results. Cut thinner tissue sections. Ice crystals may have destroyed morphology of frozen sections. Increase fixation time and/or add a post-fixation step. Increase the fixative/tissue ratio. Cut smaller pieces of tissue for more thorough immersion fixation. Increase the fixation time, ratio. Consider using cross-linking fixative.
Problem: Staining is Inappropriate

Possible Source
Fixation method is inappropriate for the antigen. Antigen retrieval may be inappropriate for this antigen or tissue. Electrostatic charge of the antigen has been altered. Delay in fixation caused diffusion of the antigen.
Test or Action
Try a different fixative or increase the fixation time. Try different antigen retrieval conditions. Try adjusting the pH or cation concentration of the antibody diluent. Fix tissue promptly. Try a cross-linking fixative rather than organic (alchohol) fixative.
21
R&D SYSTEMS IHC/ICC ANTIBODIES

MOLECULE SPECIES Human 2B4/CD244/SLAMF4 Mouse Human Human Multi-species Human Human Mouse LABEL None None Biotin None Biotin None None None None None CLONE Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 198960 499325 Polyclonal 524731 Polyclonal HOST/TYPE Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG2B Mouse IgG1 Sheep IgG Mouse IgG1 Sheep IgG CATALOG # AF1039 AF1050 BAF1050 AF838 BAF838 MAB3249 MAB5858 AF4975 MAB4975 AF5049 SIZE 100 g 100 g 50 g 100 g 50 g 100 g 100 g 100 g 100 g 100 g PRICE $355 $355 $380 $355 $380 $240 $240 $355 $240 $355
4-1BB/TNFRSF9/CD137 4-Hydroxynonenal 5HT1B
5T4
5T4 in Human Placenta. 5T4 was detected in paraffin-embedded human placenta sections using antihuman 5T4 affinity-purified polyclonal antibody (Catalog # AF4975). Tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013). Tissue was stained using anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).
8-oxo-dG A2B5 Human/Mouse/Rat/Chicken
None None
2E2 105
Mouse IgG2B Mouse IgM
4354-MC-050 MAB1416
50 L 100 g
$415 $295
A2B5 in Human differentiating NTera2 Cells. NTera2 neuronal carcinoma cells were differentiated with retinoic acid for 10 days. A2B5 was detected using anti-human/mouse/rat/chicken A2B5 monoclonal antibody (Catalog # MAB1416). The cells were stained using NorthernLights-557 anti-mouse IgG secondary antibody (Catalog # NL007; red) and the nuclei counterstained with DAPI (blue).
a1-Acid Glycoprotein a-2-Macroglobulin
Human Human
None None Biotin None
386131 Polyclonal Polyclonal Polyclonal 129736 129736 Polyclonal 402104 Polyclonal 5D3 5D3 Polyclonal Polyclonal Polyclonal Polyclonal
Mouse IgG2A Goat IgG Goat IgG Goat IgG Mouse IgG1 Mouse IgG1 Goat IgG Rat IgG2A Goat IgG Mouse IgG2B Mouse IgG2B Goat IgG Goat IgG Goat IgG Goat IgG
MAB3694 AF1938 BAF1938 AF586 BAM586 MAB586 AF3080 MAB3080 AF2756 BAM995 MAB995 AF929 BAF929 AF1513 BAF1513
100 g 100 g 50 g 100 g 250 g 500 g 100 g 100 g 100 g 250 g 500 g 100 g 50 g 100 g 50 g
$240 $355 $380 $355 $380 $325 $355 $240 $355 $380 $325 $355 $380 $355 $380
a2u-Globulin
Rat
Biotin None
A33
Human Mouse
None None None Biotin None None
ABCG2
Human
ACE/CD143
Human
Biotin None
Mouse
Biotin
ACE in Human Kidney. Angiotensin Converting Enzyme (ACE) was detected in paraffin-embedded human kidney tissue sections using anti-human ACE polyclonal antibody (Catalog # AF929). Tissues were stained using the Goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
22

MOLECULE SPECIES LABEL None ACE-2 Human Biotin None Mouse None CLONE Polyclonal Polyclonal 171606 Polyclonal HOST/TYPE Goat IgG Goat IgG Mouse IgG2A Goat IgG CATALOG # AF933 BAF933 MAB933 AF3437 SIZE 100 g 50 g 500 g 100 g PRICE $355 $380 $325 $355
ACE-2 in Mouse Kidney. Angiotensin Converting Enzyme-2 (ACE-2) was detected in a cryostat section of mouse kidney using anti-mouse affinity-purified ACE-2 polyclonal antibody (Catalog # AF3437; red). The tissue was counterstained green.
Actin Activin A bA subunit Activin C bC subunit Activin RIA/ALK-2 Activin RIB/ALK-4 Activin RIIA Activin RIIB ADAM8 Ectodomain ADAM9 Ectodomain ADAM10 Ectodomain
Human/Mouse/Rat Human/Mouse/Rat Human Human Mouse Human Human Human Mouse Human Mouse
None None None None None None None None None None None None None
Polyclonal Polyclonal Polyclonal Polyclonal 71412 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 23G9 Polyclonal
Sheep IgG Goat IgG Goat IgG Goat IgG Mouse IgG1 Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Total Goat IgG Total Goat IgG Goat IgG Goat IgG Mouse IgG1 Goat IgG
AF4000 AF338 AF1629 AF637 MAB637 AF1477 AF340 AF339 AF1031 AF949 AB936 AB946 AF935 BAF935 MAB935 AF945
100 g 100 g 100 g 100 g 500 g 100 g 100 g 100 g 100 g 100 g 1 mg 1 mg 100 g 50 g 500 g 100 g
$355 $355 $355 $355 $325 $355 $355 $355 $355 $355 $355 $355 $355 $380 $325 $355
ADAM15 Ectodomain
Human
Biotin None
Mouse
None
ADAM15 Expression in Mouse Cerebellum. A disintegin and mettalloprotease domain 15 (ADAM15) was detected in cryostat sections of mouse cerebellar Purkinje cells using anti-mouse ADAM15 polyclonal antibody (Catalog # AF945; red). The tissue was counterstained green.
ADAM23 aa 60-585
Human
None
504425
Mouse IgG2A
MAB4974
100 g
$240
ADAM23 in Human Spleen. A disintegrin and metalloprotease domain 23 (ADAM23) was detected in paraffin-embedded human spleen sections using anti-human ADAM23 monoclonal antibody (Catalog # MAB4974). Tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013). Tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).
ADAM32 aa 17-476
Human
None
539220
Mouse IgG1
MAB5380
100 g
$240
23

MOLECULE ADAMTS1 Propeptide SPECIES Human LABEL None CLONE Polyclonal HOST/TYPE Goat IgG CATALOG # AF3079 SIZE 100 g PRICE $355
ADAMTS1 in Human Placenta. A disintegrin-like and mettaloprotease with thrombospondin motifs (ADAMTS1) was detected in a paraffin-embedded human placenta section using anti-human ADAMTS1 affinity-purified polyclonal antibody (Catalog # AF3079). The tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Adiponectin/Acrp30 Adiponutrin/ADPN
Mouse Rat Human
None None None
Polyclonal Polyclonal Polyclonal
Goat IgG Goat IgG Sheep IgG
AF1119 AF3100 AF5208
100 g 100 g 100 g
$355 $355 $355
ADPN in Human Adipocytes. Adiponutrin (ADPN) was detected in human adipocytes using anti-human ADPN affinity-purified polyclonal antibody (Catalog # AF5208). Cells were stained using NorthernLights-557 donkey anti-sheep IgG secondary antibody (Catalog # NL010; red) and the nuclei counterstained with DAPI (blue).
Human a-Fetoprotein/AFP Human/Mouse Mouse Aggrecan G1-IGD-G2 Domains Human Human Mouse
None None None None None None None None
Polyclonal 189506 189502 Polyclonal Polyclonal 179509 Polyclonal Polyclonal
Chicken IgY Mouse IgG1 Mouse IgG1 Goat IgG Goat IgG Mouse IgG2B Goat IgG Goat IgG
AF1369 MAB1369 MAB1368 AF5369 AF1220 MAB1220 AF704 AF634
100 g 100 g 100 g 100 g 100 g 500 g 100 g 100 g
$355 $240 $240 $355 $355 $325 $355 $355
AgRP/ART
AgRP in Mouse Thalamus. Agouti-Related Protein (AgRP) was detected in a cryostat section of mouse thalamus using anti-mouse AgRP monoclonal antibody (MAB634; red). The tissue was counterstained green.
AIF a-Internexin Akt Pan Specific Phospho-Akt (S473) Pan Specific Akt1 Akt2 Serum Albumin ALCAM/CD166
Human/Mouse/Rat Human Human/Mouse/Rat Human/Mouse/Rat Human Human Human/Mouse/Rat Human/Mouse Mouse
None None None None None None None None None None Biotin None Biotin
Polyclonal Polyclonal 529724 281046 Polyclonal 302407 302501 Polyclonal 188835 Polyclonal Polyclonal Polyclonal 118311
Rabbit IgG Sheep IgG Mouse IgG1 Mouse IgG2B Rabbit IgG Mouse IgG1 Mouse IgG1 Goat IgG Mouse IgG2A Goat IgG Goat IgG Goat IgG Mouse IgG2A
AF1457 AF5824 MAB4844 MAB2055 AF887 MAB17751 MAB23152 AF23151 MAB1455 AF1172 BAF1172 AF577 BAM5771
100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 50 g 100 g 100 g
$285 $355 $240 $240 $285 $240 $240 $355 $240 $355 $380 $355 $240
ALK-7
Rat
24

MOLECULE Alkaline Phosphatase/ALPL SPECIES Human/Mouse/Rat LABEL Biotin None CLONE B4-78 B4-78 HOST/TYPE Mouse IgG1 Mouse IgG1 CATALOG # BAM1448 MAB1448 SIZE 100 g 100 g PRICE $240 $240
Alkaline Phosphatase and Oct-3/4 in Human BG01V Embryonic Stem Cells. Alkaline Phosphatase was detected in the human BG01V embryonic stem cell line using anti-human/mouse/rat Alkaline Phosphatase monoclonal antibody (Catalog # MAB1448). Oct-3/4 was detected using anti-human Oct-3/4 polyclonal antibody (Catalog # AF1759). The cells were stained for Alkaline Phosphatase using NorthernLights-557 anti-mouse secondary antibody (Catalog # NL007; pseudocolored green) and stained for Oct-3/4 using NorthernLights-637 anti-goat secondary antibody (Catalog # NL002; red).
AMIGO2 AMIGO3
Human Mouse
None None
273610 371301
Mouse IgG3 Rat IgG2A
MAB20801 MAB2375
100 g 100 g
$240 $240
AMIGO3 in Mouse Cerebellum. Amphoterin-induced Gene and ORF 3 (AMIG03) was detected in Purkinje neuron cell bodies in a cryostat section of mouse cerebellum using anti-mouse AMIG03 monoclonal antibody (Catalog # MAB2375).
Aminopeptidase N/ANPEP
Human Mouse
None None
498001 Polyclonal
Mouse IgG2A Goat IgG
MAB3815 AF2335
100 g 100 g
$240 $355
Aminopeptidase N/CD13 in Human Liver Cancer. Aminopeptidase N (APN) was detected in paraffinembedded human liver cancer sections using antihuman APN monoclonal antibody (Catalog # MAB3815). Tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).
Aminopeptidase O/ONPEP Aminopeptidase P1/XPNPEP1
Human Human Human Mouse
None None None None None None None None None None None None
Polyclonal 409124 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal H7507 Polyclonal Polyclonal 171718
Goat IgG Mouse IgG1 Total Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG2A Goat IgG Goat IgG Mouse IgG2B
AF4647 MAB29701 AB-262-NA AF262 AF989 AF3197 AF2850 AF2854 PP-H7507-00 AF265 AF923 MAB923
100 g 100 g 1 mg 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 500 g
$355 $240 $355 $355 $355 $355 $355 $355 $380 $355 $355 $325
Amphiregulin
AMPKa1 aa 340-550 AMPKa2 aa 340-552 AMPKb1 Androgen R/NR3C4 Angiogenin Angiopoietin-1
Human/Mouse/Rat Human/Mouse/Rat Human/Mouse/Rat Human Human Human
25

MOLECULE Angiopoietin-2 SPECIES Human LABEL None CLONE Polyclonal HOST/TYPE Goat IgG CATALOG # AF623 SIZE 100 g PRICE $355
Angiopoietin-2 in Human Gastrointestinal Cancer. Angiopoietin-2 was detected in paraffin-embedded human gastrointestinal cancer sections using anti-human Angiopoietin-2 affinity-purified polyclonal antibody (Catalog # AF623). Tissue was stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Angiopoietin-3 Angiopoietin-4 Angiopoietin-like 1 Angiopoietin-like 2 Angiopoietin-like 3 ANP AP-2g APC C-Terminus APJ APLP-1
Mouse Human Human Mouse Mouse Human Human Human Human Human
None None None None None Biotin None None None None None None None None None None
Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 361101 Polyclonal Polyclonal 72133 Polyclonal 354008 Polyclonal 369717 Polyclonal 304904
Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Rat IgG2B Goat IgG Goat IgG Mouse IgG3 Goat IgG Mouse IgG2B Goat IgG Mouse IgG2B Goat IgG Mouse IgG1
AF738 AF964 AF2204 AF1444 AF136 BAF136 MAB3366 AF5059 AF3695 MAB856 AF3129 MAB3129 AF3556 MAB4124 AF1168 MAB2508
100 g 100 g 100 g 100 g 100 g 50 g 100 g 100 g 100 g 500 g 100 g 100 g 100 g 100 g 100 g 100 g
$355 $355 $355 $355 $355 $380 $240 $355 $355 $325 $355 $240 $355 $240 $355 $240
Apolipoprotein B/ApoB APP Pan Specific Phospho-APP (T668)
Human Human Human/Mouse
Phospho-Amyloid Precursor Protein (668T) in Human Alzheimers Disease Brain. Amyloid precursor protein (APP) phosphorylated at T668 was detected in paraffin-embedded human Alzheimers disease brain sections using anti-human phospho-APP monoclonal antibody (Catalog # MAB2508). Tissue was stained using anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).
ARC aa 176-195 b-Arrestin 1 b-Arrestin 2
Human/Mouse/Rat Human Human Human
None None None None None None
Polyclonal Polyclonal 425817 Polyclonal Polyclonal Polyclonal Polyclonal 185234 Polyclonal Polyclonal Polyclonal Polyclonal
Rabbit IgG Goat IgG Rat IgG2A Goat IgG Goat IgG Goat IgG Goat IgG Rat IgG2A Goat IgG Sheep IgG Rabbit IgG Goat IgG
AF636 AF4560 MAB45601 AF4564 AF2589 AF1085 BAF1085 MAB10851 AF2485 AF3575 PPS064 AF154
100 g 100 g 100 g 100 g 100 g 100 g 50 g 500 g 100 g 100 g 100 L 100 g
$285 $355 $240 $355 $355 $355 $380 $325 $355 $355 $355 $355
Artemin
Mouse
Biotin None
Arylsulfatase A/ARSA ASK1 aa 1011-1196 Phospho-ATF2 (S490/S498) Axl
Human Human Human/Rat Human
None None None None
26

MOLECULE Phospho-Axl (Y779) SPECIES Human LABEL None CLONE Polyclonal HOST/TYPE Rabbit IgG CATALOG # AF2228 SIZE 100 g PRICE $285
Phospho-Axl (Y779) in Human Stomach. PhosphoAxl was detected in paraffin-embedded human stomach sections using anti-human/mouse phospho-Axl affinity-purified polyclonal antibody (Catalog # AF2228). Tissue was stained using anti-rabbit HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS005; brown) and counterstained with hematoxylin (blue).
B220/CD45R
Mouse
Biotin None None None None None Biotin None None Biotin None None None Biotin None None None Biotin None None None None None None None None None
RA3-6B2 RA3-6B2 Polyclonal 37711 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 137612 391017 Polyclonal Polyclonal 542515 409633 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal YTH-2H12 Polyclonal Polyclonal 35928
Rat IgG2A Rat IgG2A Goat IgG Mouse IgG1 Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG1 Rat IgG2A Goat IgG Goat IgG Mouse IgG1 Mouse IgG1 Goat IgG Rabbit IgG Total Goat IgG Goat IgG Goat IgG Mouse IgG2A Chicken IgY Chicken IgY Mouse IgG1
BAM1217 MAB1217 AF140 MAB140 AF740 AF1214 BAF1214 AF-141-NA AF1340 BAF1340 AF1019 AF158 AF1027 BAF1027 MAB931 MAB4097 AF124 BAF2106 MAB4969 MAB39651 AF921 AF820 AB820 AF810 AF8241 2300-MC-100 AF248 BAF248 MAB248
250 g 500 g 100 g 500 g 100 g 100 g 50 g 200 g 100 g 50 g 100 g 100 g 100 g 50 g 500 g 100 g 100 g 50 g 100 g 100 g 100 g 30 g 400 g 100 g 100 g 100 g 100 g 50 g 500 g
$380 $325 $355 $325 $355 $355 $380 $230 $355 $380 $355 $355 $355 $380 $325 $240 $355 $380 $240 $240 $355 $285 $395 $355 $355 $285 $355 $380 $325
Human B7-1/CD80 Mouse Rat Human B7-2/CD86 Rat Mouse Mouse Human Human/Mouse Human Human Mouse Human Human Human Human/Mouse Human Human/Mouse Human/Mouse Human/Mouse/Rat
B7-H1/PD-L1 B7-H2 B7-H3 BACE-1 Ectodomain BACE-2 Ectodomain BAFF/BLyS/TNFSF13B BAI1 BAI3 BAMBI/NMA Bax aa 12-33 Bax Minus C-Terminus Bcl-2 aa 1-208 Bcl-w aa 1-172 Bcl-xL aa 3-14
BDNF
Human
Biotin None
BDNF in Rat Spinal Cord and Dorsal Root Ganglia. Brain-derived neurotrophic factor (BDNF) was detected in rat dorsal root ganglion using anti-human BDNF affinity-purified polyclonal antibody (Catalog # AF248; red) and counterstained green.
Human Betacellulin/BTC Mouse Mouse
None None Biotin None
Polyclonal Polyclonal Polyclonal Polyclonal
Goat IgG Goat IgG Goat IgG Sheep IgG
AF-261-NA AF1025 BAF1025 AF2559
100 g 100 g 50 g 100 g
$355 $355 $380 $355
bIG-H3
27

MOLECULE Biglycan SPECIES Human LABEL None CLONE Polyclonal HOST/TYPE Goat IgG CATALOG # AF2667 SIZE 100 g PRICE $355
Biglycan in Human Skin. Biglycan was detected in paraffin-embedded human skin sections using antihuman Biglycan affinity-purified polyclonal antibody (Catalog # AF2667). Tissue was stained using antigoat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
BIK BMI-1 BMP-1/PCP Pro BMP-2 BMP-2/BMP-4 BMP-3 BMP-3b/GDF-10 BMP-5 BMP-6 BMP-7 BMP-8 BMP-15/GDF-9B aa 268-392
Human Human Human Human Human Human Human Human Human Human Human Human
None None None None None Biotin None None None Biotin None None None None None
Polyclonal Polyclonal Polyclonal 253717 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 164311 164313 Polyclonal 317809
Sheep IgG Goat IgG Goat IgG Mouse IgG2A Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG2B Mouse IgG1 Goat IgG Rat IgG2A
AF5474 AF3334 AF1927 MAB2260 AF355 BAF355 AF113 AF1543 AF615 BAF615 AF507 MAB3541 MAB3542 AF1073 MAB2925
$355 $355 $355 $325 $355 $380 $355 $355 $355 $380 $355 $325 $325 $355 $240
BMP-15/GDF-9b in Mouse Ovary. Bone Morphogenetic Protein-15 (BMP-15) was detected in a frozen section of adult mouse ovary using anti-human BMP15 monoclonal antibody (Catalog # MAB2925). Cells were stained using NorthernLights-557 goat anti-rat IgG secondary antibody (red; Catalog # NL013) and counterstained with DAPI (blue).
BMPR-IA/ALK-3 BMPR-IB/ALK-6 BMPR-II
Human Human Human
None None None Biotin
550607 477914 Polyclonal Polyclonal
Mouse IgG2B Mouse IgG2B Goat IgG Goat IgG
MAB24061 MAB5051 AF811 BAF811
100 g 100 g 100 g 50 g
$240 $240 $355 $380
BMPR-II in Mouse Embryo. BMPR-II was detected in a frozen section of E15 mouse ribs using anti-human BMPR-II affinity-purified polyclonal antibody (Catalog # AF811; green).
BNP Precursor BOC
Human Mouse
None None
370124 Polyclonal
Rat IgG2B Goat IgG
MAB36041 AF2385
100 g 100 g
$240 $355
28

MOLECULE Brachyury SPECIES Human LABEL None CLONE Polyclonal HOST/TYPE Goat IgG CATALOG # AF2085 SIZE 100 g PRICE $355
Brachyury in Differentiated Human Embryonic Stem cells. Brachyury was detected in differentiated human Embryonic Stem cells using anti-human Brachyury affinity-purified polyclonal antibody (Catalog # AF2085). Cells were stained using an anti-goat secondary antibody (green) and counterstained with DAPI (blue). Courtesy of Dr. Frank Solder from the National Institute of Neurological Disorders and Stroke & Stem Cell Unit at NIH.
Brachyury in Embryonic Mouse Notochord. Brachyury was detected in E9.5 mouse notochord using anti-human Brachyury affinity-purified polyclonal antibody (Catalog # AF2085). The cells were stained using NorthernLights-557 secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).
Brachyury NorthernLights-conjugated BRCA1 Phospho-BRCA1 (S1423) BRCA2 Brevican Brk aa 1-70 BSRP-A
Human Human Human/Mouse/Rat Human Human Human Human Mouse
NL557 None None None None None Biotin None None
Polyclonal Polyclonal 265402 Polyclonal 234403 Polyclonal Polyclonal Polyclonal 529805
Goat IgG Goat IgG Mouse IgG2A Rabbit IgG Mouse IgG1 Sheep IgG Sheep IgG Goat IgG Rat IgG2A
NL2085R AF2210 MAB2210 AF1386 MAB2476 AF4009 BAF4009 AF3966 MAB4916
500 L 100 g 100 g 100 g 100 g 100 g 50 g 100 g 100 g
$350 $355 $240 $285 $240 $355 $380 $355 $240
BSRP-A in Mouse Hippocampus. Brain-specific receptor-like protein A (BSRP-A) was detected in a cryostat section of mouse hippocampus using anti mouse BSRP-A monoclonal antibody (Catalog # MAB4916). The tissue was stained using the anti-rat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS017; brown) and counterstained with hematoxylin (blue).
C1q R1/CD93 C4.4A/LYPD3
Human Mouse Mouse
None None None None
Polyclonal 223437 Polyclonal Polyclonal 427909 427903
Goat IgG Rat IgG2B Goat IgG Sheep IgG Mouse IgG1 Mouse IgG3
AF2379 MAB1696 AF5567 AF2715 MAB2715 MAB27151
100 g 100 g 100 g 100 g 100 g 100 g
$355 $240 $355 $355 $240 $240
Cadherin-6/KCAD
Human
None None
K-Cadherin in Human Liver Cancer. K-Cadherin was detected in paraffin-embedded human liver cancer sections using anti-human K-cadherin monoclonal antibody (Catalog # MAB27151). The tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).
Cadherin-11 Cadherin-12
Human Human
None None
Polyclonal 343621
Goat IgG Rat IgG1
AF1790 MAB2240
100 g 100 g
$355 $240
29

MOLECULE Cadherin-13 SPECIES Human LABEL None CLONE Polyclonal HOST/TYPE Goat IgG CATALOG # AF3264 SIZE 100 g PRICE $355
Cadherin-13 in Human Lung Cancer Cells. Cadherin-13 was detected in NCI-H460 human lung cancer cell line using anti-human Cadherin-13 affinitypurified polyclonal antibody (Catalog # AF3264). Cells were stained using NorthernLights-557 antigoat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).
None Cadherin-17 Human Biotin None None Human E-Cadherin Mouse None None None Biotin None
E-Cadherin and SOX2 in the BG01V Human Embryonic Stem Cell Line. E-Cadherin and SOX2 were detected in human BG01V cells using antihuman E-Cadherin affinity-purified polyclonal antibody (Catalog # AF648; red) and anti-human/ mouse SOX2 monoclonal antibody (Catalog # MAB2018; green). Cells were stained using anti-goat NorthernLights-557 (Catalog # NL001) and antimouse NorthernLights-493 (Catalog # NL009). Cells were counterstained with DAPI (blue).
Polyclonal Polyclonal 141713 Polyclonal 180215 180224 Polyclonal Polyclonal 114420
Goat IgG Goat IgG Mouse IgG1 Goat IgG Mouse IgG2B Mouse IgG2B Goat IgG Goat IgG Rat IgG2A
AF1032 BAF1032 MAB1032 AF648 MAB1838 MAB18381 AF748 BAF748 MAB7481
100 g 50 g 500 g 100 g 500 g 100 g 100 g 50 g 100 g
$355 $380 $325 $355 $325 $240 $355 $380 $240
N-Cadherin
Human
None
Polyclonal
Sheep IgG
AF1388
100 g
$355
N-Cadherin in Human Hippocampus. N-Cadherin was detected in a paraffin-embedded section of human hippocampus using anti-human N-Cadherin affinity-purified polyclonal antibody (Catalog # AF1388). The tissue was stained using anti-sheep HRP-DAB detection reagents (brown) and counterstained with hematoxylin (blue).
Human P-Cadherin Mouse
None None None None None None None
104805 Polyclonal 106020 Polyclonal 123413 Polyclonal 401025
Mouse IgG1 Goat IgG Rat IgG2A Goat IgG Mouse IgG2B Goat IgG Mouse IgG2B
MAB861 AF761 MAB761 AF938 MAB9381 AF3320 MAB3320
500 g 100 g 500 g 100 g 100 g 100 g 100 g
$325 $355 $325 $355 $240 $355 $240
VE-Cadherin
Human
Calbindin D
Human
Calbindin D in Rat Hypothalamus. Calbindin D was detected in a frozen rat hypothalamus section using anti-human calbindin D monoclonal antibody (Catalog # MAB3320). Tissue was stained using anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).
30

MOLECULE Calretinin SPECIES Human/Mouse/Rat LABEL None CLONE Polyclonal HOST/TYPE Goat IgG CATALOG # AF5065 SIZE 100 g PRICE $355
Calretinin/Calbindin-2 in Human Brain Cortex. Calretinin was detected in paraffin-embedded human brain cortex sections using anti-human/mouse/ rat Calretinin affinity-purified polyclonal antibody (Catalog # AF5065). The tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Calsyntenin-3 CaM Kinase IIa CaM Kinase IId aa 402-478
Human Human Human
None None None
Polyclonal 525501 Polyclonal
Sheep IgG Mouse IgG2B Sheep IgG
AF5244 MAB5584 AF4176
100 g 100 g 100 g
$355 $240 $355
CaMKIId in Human Cerebral Cortex. Calcium/ Calmodulin-dependent protein kinase type II delta (CaMKIId) was detected in paraffin-embedded hum a n cerebral cortex tissue sections using anti-human CaMKIId affinity-purified polyclonal antibody (Catalog # AF4176). Tissues were stained using antisheep HRP-DAB detection (brown) and counterstained with hematoxylin (blue).
cAMP Cannabinoid R2/CB2/CNR2 CAR/NR1I3 Carbonic Anhydrase II
Multi-species Human Human Human Human Mouse
None None None None None None None None None None None None Biotin None None None None None None
250532 352114 N4111 Polyclonal Polyclonal 310413 Polyclonal Polyclonal Polyclonal Polyclonal 352216 Polyclonal Polyclonal 113612 Polyclonal 269518 LAP6 Polyclonal Polyclonal
Mouse IgG1 Mouse IgG2A Mouse IgG1 Sheep IgG Goat IgG Mouse IgG2B Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG1 Goat IgG Goat IgG Mouse IgG2B Rabbit IgG Rabbit IgG Mouse IgG1 Goat IgG Sheep IgG
MAB2146 MAB3655 PP-N4111-00 AF2184 AF2186 MAB2186 AF2414 AF2188 AF2344 AF2489 MAB2489 AF163 BAF163 MAB163 AF835 MAB835 MAB8301 AF8301 AF5145
500 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 50 g 500 g 50 g 50 g 100 g 100 g 100 g
$325 $240 $380 $355 $355 $240 $355 $355 $355 $355 $240 $355 $380 $325 $285 $270 $240 $355 $355
Carbonic Anhydrase IV
Carbonic Anhydrase IX
Human Mouse Human
Carnosine Dipeptidase 1/CNDP1
CART CART aa 28-116 Active Caspase-3 aa 163-175 Cleaved Caspase-3 (Asp175) Caspase-9 aa 1-134 Caspase-9 aa 139-330 Caspr2
Human Human Human/Mouse Human/Mouse Human Human Human
Caspr2 in Mouse Neurons. Caspr2 was detected in a frozen section of adult mouse brain using anti-human Caspr2 affinity-purified polyclonal antibody (Catalog # AF5145). Cells were stained using NorthernLights-557 donkey anti-sheep IgG secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue).
31

MOLECULE SPECIES Human Human/Mouse/Rat LABEL None None None CLONE 196618 196618 Polyclonal HOST/TYPE Mouse IgG3 Mouse IgG3 Goat IgG CATALOG # MAB13291-050 MAB13291-100 AF1329 SIZE 50 g 100 g 100 g PRICE $150 $240 $355
b-Catenin
b-Catenin in Human Kidney Cancer. b-Catenin was detected in paraffin-embedded human kidney cancer sections using anti-human/mouse/rat b-Catenin affinity-purified polyclonal antibody (Catalog # AF1329). Tissue was stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Cathepsin A/Lysosomal Carboxypeptidase A
Human Mouse Human
None Biotin None None Biotin None Biotin None None Biotin None Biotin None Biotin None Biotin None Biotin None None None
Polyclonal Polyclonal 191424 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 237208 Polyclonal
Goat IgG Goat IgG Rat IgG2B Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG1 Goat IgG
AF1049 BAF1049 MAB11231 AF953 BAF953 AF965 BAF965 AF1071 AF1034 BAF1034 AF1014 BAF1014 AF1029 BAF1029 AF1294 BAF1294 AF1130 BAF1130 AF2075 MAB2075 AF2544
100 g 50 g 500 g 100 g 50 g 100 g 50 g 100 g 100 g 50 g 100 g 50 g 100 g 50 g 100 g 50 g 100 g 50 g 100 g 500 g 100 g
$355 $380 $325 $355 $380 $355 $380 $355 $355 $380 $355 $380 $355 $380 $355 $380 $355 $380 $355 $325 $355
Cathepsin B Mouse Human Cathepsin C/DPPI Mouse
Human Cathepsin D Mouse
Human Cathepsin E Mouse
Cathepsin F
Human Mouse
Cathepsin F in Human Heart. Cathepsin F was detected in paraffin-embedded human heart tissue sections using anti-human Cathepsin F monoclonal antibody (Catalog # MAB2075). Tissues were stained using anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).
Cathepsin H
Mouse
None
Polyclonal
Goat IgG
AF1013
100 g
$355
32

MOLECULE SPECIES Human Cathepsin L Human/Mouse Mouse LABEL None None None None CLONE Polyclonal 204106 204101 Polyclonal HOST/TYPE Goat IgG Rat IgG2B Rat IgG2A Goat IgG CATALOG # AF952 MAB952 MAB9521 AF1515 SIZE 100 g 500 g 500 g 100 g PRICE $355 $325 $325 $355
Cathepsin L in Mouse Ovary. Cathepsin L was detected in frozen sections of mouse ovary using anti-mouse Cathepsin L affinity-purified polyclonal antibody (Catalog # AF1515). Tissue was stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Cathepsin O Cathepsin S
Human Human
None None Biotin None None None Biotin None Biotin None None None
Polyclonal Polyclonal Polyclonal Polyclonal 182016 Polyclonal Polyclonal Polyclonal Polyclonal 257003 Polyclonal 525222
Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG1 Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG2B Goat IgG Mouse IgG1
AF1174 AF1183 BAF1183 AF1080 MAB1080 AF934 BAF934 AF1033 BAF1033 MAB2676 AF5599 MAB4499
100 g 100 g 50 g 100 g 500 g 100 g 50 g 100 g 50 g 100 g 100 g 100 g
$355 $355 $380 $355 $325 $355 $380 $355 $380 $240 $355 $240
Cathepsin V
Human
Human Cathepsin X/Z/P Mouse CBP Ccd1/DIXDC1 CCK4 aa 31-199 Human/Mouse/Rat Mouse Human/Rat
CCK4 in Human Colon Cancer. Colon carcinoma kinase 4 (CCK4) was detected in a paraffin-emedded section of human colon cancer using anti-human CCK4 monoclonal antibody (Catalog # MAB4499). The tissue was stained using the anti-mouse HRPDAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).
CCK-A R aa 256-267
Human/Mouse/Rat
None None
Polyclonal Polyclonal Polyclonal 280702 23002 Polyclonal 93321 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 53405
Rabbit IgG Goat IgG Goat IgG Mouse IgG1 Mouse IgG2B Goat IgG Mouse IgG1 Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Rat IgG2A
AF2680 AF1774 BAF1774 MAB28171 MAB2791 BAF270 MAB270 AF-450-NA AF-271-NA BAF271 AF-451-NA BAF451 AF-278-NA AF478 MAB478
$285 $355 $380 $325 $325 $380 $325 $355 $355 $380 $355 $380 $355 $355 $325
CCL2/JE/MCP-1
Canine
Biotin None
Human Human Mouse Human CCL4/MIP-1b Mouse Human CCL5/RANTES Mouse
None Biotin None None None Biotin None Biotin None None None
CCL3/MIP-1a
33

MOLECULE SPECIES Human LABEL None None None Mouse Biotin Biotin None CCL16/HCC-4 CCL17/TARC CCL19/MIP-3b Human Human Human Mouse Human Human Human Mouse Mouse Human None None None None None None Biotin None None None None None None Human CCL27/CTACK Mouse None None None Biotin None
CCL27/CTACK in Normal Human Skin. CCL27/ CTACK was detected in a frozen tissue section of normal human skin using anti-human CCL27/CTACK affinity-purified polyclonal antibody (Catalog # AF376). Tissues were stained using the anti-goat HRP-AEC Cell and Tissue Staining Kit (Catalog # CTS009; red) and counterstained with hematoxylin (blue).
CLONE Polyclonal 43911 Polyclonal Polyclonal 42285 42285 Polyclonal 70218 Polyclonal 54909 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 89827 Polyclonal Polyclonal 124308 124302 Polyclonal 68706 68706
HOST/TYPE Goat IgG Mouse IgG1 Goat IgG Goat IgG Rat IgG2A Rat IgG2A Goat IgG Mouse IgG1 Goat IgG Mouse IgG2B Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Rat IgG2A Goat IgG Goat IgG Mouse IgG1 Mouse IgG2A Goat IgG Rat IgG2A Rat IgG2A
CATALOG # AF-320-NA MAB320 AF-420-NA BAF420 BAM420 MAB420 AF802 MAB328 AF364 MAB361 AF880 AF360 BAF360 AF366 AF343 AF-481-NA MAB4811 AF653 AF376 MAB376 MAB3761 AF725 BAM7251 MAB7251
SIZE 100 g 500 g 100 g 50 g 250 g 500 g 100 g 500 g 100 g 500 g 100 g 100 g 50 g 100 g 100 g 100 g 500 g 100 g 100 g 500 g 500 g 100 g 250 g 500 g
PRICE $355 $325 $355 $380 $380 $325 $355 $325 $355 $325 $355 $355 $380 $355 $355 $355 $325 $355 $355 $325 $325 $355 $380 $325
CCL11/Eotaxin
CCL20/MIP-3a CCL21/6Ckine CCL24/Eotaxin-2/MPIF-2 CCL25/TECK CCL25/TECK CCL26/Eotaxin-3
CCR1 CCR3 CCR5
Human Human Human Human Mouse Human Mouse
53504 61828 45523 45549 53103 140706 150503 4B12
Mouse IgG2B Rat IgG2A Mouse IgG2B Mouse IgG2B Mouse IgG2B Rat IgG2A Mouse IgG2A Rat IgG2A
MAB145 MAB155 MAB181 MAB183 MAB195 MAB590 MAB197 MAB3477
500 g 500 g 500 g 500 g 500 g 500 g 500 g 100 g
$325 $325 $325 $325 $325 $325 $325 $240
CCR6
CCR7
CCR7 in Human Peripheral Blood Mononuclear Cells. CCR7 was detected in human PBMCs using anti-human CCR7 monoclonal antibody (Catalog # MAB197; red). Cells were counterstained green.
CCR9
Human
None None
112509 248621
Mouse IgG2A Mouse IgG2A
MAB179 MAB1791
500 g 500 g
$325 $325
34

MOLECULE CD2 CD3 SPECIES Human Mouse LABEL None Biotin None CLONE 299812 17A2 17A2 HOST/TYPE Mouse IgG1 Rat IgG2B Rat IgG2B CATALOG # MAB18561 BAM4841 MAB4841 SIZE 100 g 250 g 500 g PRICE $240 $380 $325
CD3-expressing Cells in Mouse Brain (perivascular cuffing). Cells expressing CD3 were detected in inflamed mouse brain using anti-mouse CD3 affinitypurified (Catalog # MAB4841; green). The nuclei were counterstained with DAPI (blue).
CD3e
Human
None
UCHT1
Mouse IgG1
MAB100
500 g
$325
CD3e in Human Peripheral Blood Mononuclear Cells (PBMCs). CD3e was detected in human PBMCs using anti-human CD3e monoclonal antibody (Catalog # MAB100). Cells were stained using NorthernLights-557 anti-mouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).
None Canine Biotin None Feline None None None None Biotin None None Biotin None None Biotin None None Biotin None None Biotin None None None None None None None None None
Polyclonal Polyclonal 296712 Polyclonal 372711 Polyclonal 34930 GK1.5 GK1.5 Polyclonal 53-7.3 53-7.3 Polyclonal 53-6.7 53-6.7 479608 Polyclonal Polyclonal 528807 Polyclonal 301731 Polyclonal Polyclonal Polyclonal 9G11 Polyclonal 240742 82111 Polyclonal
Goat IgG Goat IgG Mouse IgG2B Goat IgG Mouse IgG2A Goat IgG Mouse IgG1 Rat IgG2B Rat IgG2B Goat IgG Rat IgG2A Rat IgG2A Goat IgG Rat IgG2A Rat IgG2A Rat IgG2A Sheep IgG Goat IgG Mouse IgG2B Goat IgG Mouse IgG2B Goat IgG Goat IgG Sheep IgG Mouse IgG1 Goat IgG Mouse IgG2A Mouse IgG2B Goat IgG
AF2410 BAF2410 MAB2410 AF2597 MAB25971 AF-379-NA MAB379 BAM554 MAB554 AF1636 BAM115 MAB115 AF2598 BAM116 MAB116 MAB5218 BAF383 AF123 MAB5248 BAF382 MAB2738 AF-342-PB AF852 AF806 BBA7 AF4117 MAB2404 MAB6321 AF440
$355 $380 $240 $355 $240 $355 $325 $380 $325 $355 $380 $325 $355 $380 $325 $240 $380 $355 $240 $380 $240 $355 $355 $355 $230 $355 $325 $325 $355
CD4
Human
Mouse Human CD5 Mouse Feline CD8a Mouse Mouse Human Human Human Human Human Human Mouse Human Rat Human Human Mouse
CD9 CD14 CD23/Fce RII CD24 CD27/TNFRSF7 CD27 Ligand/TNFSF7 CD28 CD30/TNFRSF8 CD31/PECAM-1 CD34 CD38 CD40/TNFRSF5
35

MOLECULE SPECIES Human CD40 Ligand/TNFSF5 Mouse CD43 CD44 H CD44 v3 CD44 v4/5 CD44 v6 Human Human Human Human Human Human CD45 Mouse LABEL None None None Biotin None None None None None Biotin None None Biotin None CD46 Human None CLONE Polyclonal 40804 Polyclonal Polyclonal 290111 2C5 3G5 3D2 2F10 2D1 2D1 Polyclonal 30-F11 30-F11 Polyclonal HOST/TYPE Goat IgG Mouse IgG2B Goat IgG Goat IgG Mouse IgG2B Mouse IgG2A Mouse IgG2B Mouse IgG1 Mouse IgG1 Mouse IgG1 Mouse IgG1 Goat IgG Rat IgG2B Rat IgG2B Goat IgG CATALOG # AF617 MAB617 AF1163 BAF1163 MAB2038 BBA10 BBA11 BBA25 BBA13 BAM1430 MAB1430 AF114 BAM114 MAB114 AF2005 SIZE 100 g 500 g 100 g 50 g 100 g 200 g 200 g 200 g 200 g 100 g 100 g 100 g 250 g 500 g 100 g PRICE $355 $325 $355 $380 $240 $315 $315 $315 $315 $295 $240 $355 $380 $325 $355
CD46 in Human Skin. CD46 was detected in paraffin-embedded human skin tissue sections using antihuman CD46 affinity-purified polyclonal antibody (Catalog # AF2005). Tissues were stained using antigoat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
CD47 CD47 N-terminal IgV-like Extracellular Domain
Human Mouse Human Mouse Human Human Human Mouse Human
None None Biotin None None None Biotin None None Biotin None None None
Polyclonal Polyclonal Polyclonal 394620 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 446703 298807 Polyclonal 298633 298614 310116 Polyclonal Polyclonal HB15e Polyclonal
Sheep IgG Goat IgG Goat IgG Mouse IgG2B Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Rat IgG1 Mouse IgG2B Goat IgG Mouse IgG2A Mouse IgG2A Rat IgG1 Goat IgG Goat IgG Mouse IgG1 Goat IgG
AF4670 AF1866 BAF1866 MAB36441 AF3327 AF2009 BAF2009 AF1689 AF1987 BAF1987 MAB5417 MAB20401 AF2359 MAB2359 MAB23591 MAB23861 AF1279 BAF1279 MAB1774 AF1437
100 g 100 g 50 g 100 g 100 g 100 g 50 g 100 g 100 g 50 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 50 g 500 g 100 g
$355 $355 $380 $240 $355 $355 $380 $355 $355 $380 $240 $240 $355 $240 $240 $240 $355 $380 $325 $355
CD48/SLAMF2
CD55/DAF CD58/LFA-3 CD59 CD63 CD68/SR-D1
CD69
Human
None None
Mouse CD72 Mouse Human Mouse
None None Biotin None None
CD83
36

MOLECULE CD109 SPECIES Human LABEL None CLONE Polyclonal HOST/TYPE Sheep IgG CATALOG # AF4385 SIZE 100 g PRICE $355
CD109 in Human Squamous Cell Carcinoma. CD109 was detected in paraffin-embedded human squamous cell carcinoma sections using anti-human CD109 affinity-purified polyclonal antibody (Catalog # AF4385). Tissue was stained using the anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).
CD151
Mouse
None None
455807 Polyclonal Polyclonal 300903 300907 Polyclonal Polyclonal 380529 Polyclonal Polyclonal Polyclonal 336445 Polyclonal Polyclonal Polyclonal Polyclonal
Rat IgG2A Goat IgG Goat IgG Mouse IgG1 Mouse IgG1 Goat IgG Goat IgG Mouse IgG2B Goat IgG Goat IgG Rabbit IgG Mouse IgG2B Goat IgG Goat IgG Sheep IgG Goat IgG
MAB4609 AF2530 BAF2530 MAB2530 MAB25301 AF2724 AF3355 MAB34141 AF2554 BAF2554 AF888 MAB1648 AF1649 AF2666 AF4515 AF5097
$240 $355 $380 $240 $240 $355 $355 $240 $355 $380 $285 $240 $355 $355 $355 $355
CD155/PVR
Human
Biotin None None
CD200
Human Mouse Human
None None None None Biotin None None None None None None
CD200 R1
Mouse Human/Mouse/Rat Human/Mouse/Rat Human/Mouse/Rat Human Mouse Human
Phospho-CDC2 (Y15) CDC25A CDC25B aa 377-566 CDCP1 CDNF
CDNF in Human Cortex. Conserved Dopamine Neurotrophic Factor (CDNF) was detected in paraffinembedded human cortex sections using anti-human CDNF affinity-purified polyclonal antibody (Catalog # AF5097). Tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013). Tissue was stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
CDO
Human Mouse
None None
Polyclonal Polyclonal
Sheep IgG Goat IgG
AF4384 AF2429
100 g 100 g
$355 $355
CDO Expression in Embryonic Mouse Retina. CAMrelated/Down-regulated by Oncogenes (CDO) was detected in a cryostat tissue section of embryonic mouse retina (13 d.p.c.) using anti-mouse CDO polyclonal antibody (Catalog # AF2429) and anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown). Tissues were counterstained with hematoxylin (blue).
CDX2 CDX4
Human Human
None None None None
Polyclonal Polyclonal 322007 Polyclonal Polyclonal 283324
Goat IgG Goat IgG Mouse IgG2B Goat IgG Goat IgG Mouse IgG1
AF3665 AF2807 MAB2807 AF2244 BAF2244 MAB22441
100 g 100 g 100 g 100 g 50 g 500 g
$355 $355 $240 $355 $380 $325
CEACAM-1/CD66a
Human
Biotin None
37

MOLECULE CEACAM-5/CD66e Cerberus 1 CFTR C-Terminus CFTR R Domain Chitinase 3-like 1 Chk2 aa 1-234 Phospho-Chk2 (T68) CHL-1/L1CAM-2 Choline Acetyltransferase/ChAT Isoform R Chondrolectin Chordin SPECIES Human Human Human Human Human Mouse Human Human Mouse Human Human Mouse Human Mouse Chorionic Gonadotropin, a Chain (a HCG) CIS-1 Claspin Claudin-3 Claudin-12 Human Human Human Human Human LABEL None None None None None None None None None None None None None None Biotin None None None None None None CLONE Polyclonal 487609 162836 24-1 13-1 321806 235303 Polyclonal 316223 Polyclonal Polyclonal 396305 Polyclonal Polyclonal Polyclonal 328905 381012 Polyclonal Polyclonal 385021 393314 HOST/TYPE Sheep IgG Mouse IgG2A Mouse IgG2B Mouse IgG2A Mouse IgG1 Rat IgG2B Rat IgG2A Rabbit IgG Rat IgG1 Goat IgG Goat IgG Mouse IgG2B Goat IgG Goat IgG Goat IgG Rat IgG2A Mouse IgG2A Goat IgG Goat IgG Mouse IgG2A Mouse IgG2A CATALOG # AF4128 MAB41281 MAB1075 MAB25031 MAB1660 MAB2599 MAB22851 AF1626 MAB2126 AF2147 AF3447 MAB2576 AF758 AF2448 BAF2448 MAB2520 MAB4169 AF3194 AF3310 MAB4620 MAB4284 SIZE 100 g 100 g 500 g 50 g 50 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 50 g 100 g 100 g 100 g 100 g 100 g 100 g PRICE $355 $240 $325 $215 $215 $240 $240 $285 $240 $355 $355 $240 $355 $355 $380 $240 $240 $355 $355 $240 $240
Chordin-Like 2/CHRDL2
Claudin-12 in Murine NS0 Cells. Claudin-12 was detected in a Claudin-12-transfected murine NS0 myeloma cell line using anti-human Claudin-12 monoclonal antibody (Catalog # MAB4284). Cells were stained using NorthernLights-557 donkey antimouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).
CL-P1/COLEC12 Clusterin Clusterin Isoform 1 CNP CNP Propeptide
Human Mouse Human Human Human
Polyclonal Polyclonal Polyclonal Polyclonal 347625 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal
Goat IgG Goat IgG Goat IgG Goat IgG Rat IgG2A Total Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG
AF2690 AF2747 AF2937 AF3127 MAB3739 AB-557-NA AF-557-NA BAF557 AF-559-NA BAF559
100 g 100 g 100 g 100 g 100 g 1 mg 100 g 50 g 100 g 50 g
$355 $355 $355 $355 $240 $355 $355 $380 $355 $380
CNTF
Rat
None Biotin
CNTF Ra
Rat
None Biotin
CNTF Ra in Rat Brain. Ciliary Neurotrophic Factor Receptor a (CNTF Ra) was detected in a cryostat tissue section of rat brain using biotinylated anti-rat CNTF Ra polyclonal antibody (Catalog # BAF559). Tissues were stained using the anti-goat HRP-AEC Cell and Tissue Staining Kit (Catalog # CTS009; red) and counterstained with hematoxylin (blue). Antigen retrieval using Antigen retrieval-Basic Reagent is recommended (Catalog # CTS013).
Coagulation Factor III/Tissue Factor
Human
None
Polyclonal
Goat IgG
AF2339
100 g
$355
38

MOLECULE SPECIES LABEL None Coagulation Factor VII Human Biotin None
Coagulation Factor VII in Human PBMCs. Coagulation Factor VII was detected in PHA-stimulated human peripheral blood mononuclear cells (PBMCs) using anti-human coagulation factor VII monoclonal antibody (Catalog # MAB2338; red). Cells were counterstained (green).
CLONE Polyclonal Polyclonal 321621
HOST/TYPE Goat IgG Goat IgG Mouse IgG1
CATALOG # AF2338 BAF2338 MAB2338
SIZE 100 g 50 g 100 g
PRICE $355 $380 $240
Collagen II Collectrin Complement Component C1qC aa 115-245 Complement Component C3d Contactin-1 Contactin-2/TAG1 Corneodesmosin COUP-TF I/NR2F1
Mouse Mouse Human Mouse Human Human Human Human
None None None None None None None None None None None None None None Biotin None None
Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 372944 Polyclonal H8132 H8132 H7147 H7147 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal
Sheep IgG Sheep IgG Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG2B Sheep IgG Mouse IgG2A Mouse IgG2A Mouse IgG2A Mouse IgG2A Goat IgG Sheep IgG Sheep IgG Goat IgG Goat IgG
AF3615 AF4965 AF3337 AF2655 AF904 AF1714 MAB1714 AF5725 PP-H8132-00 PP-H8132-10 PP-H7147-00 PP-H7147-10 AF4198 AF1707 BAF1707 AF1829 AF1744
100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 50 g 100 g 100 g
$355 $355 $355 $355 $355 $355 $240 $355 $380 $380 $380 $380 $355 $355 $380 $355 $355
COUP-TF II/NR2F2 COX-2 aa 18-604
Human Human/Mouse Human
C-Reactive Protein/CRP
Mouse Rat
C-Reactive Protein in Human Kidney Cancer. CReactive Protein (CRP) was detected in paraffinembedded human kidney cancer tissue sections using anti-human CRP affinity-purified polyclonal antibody (Catalog # AF1707). Tissues were stained using anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).
CREB aa 136-341 CRELD1 CRHBP CRHR-1
Human Mouse Human Mouse Human
Polyclonal 321120 Polyclonal Polyclonal Polyclonal 343919 Polyclonal Polyclonal 237603 89646 295203 Polyclonal 221428 Polyclonal
Goat IgG Mouse IgG1 Goat IgG Goat IgG Goat IgG Mouse IgG2A Sheep IgG Sheep IgG Rat IgG2B Mouse IgG1 Mouse IgG1 Goat IgG Rat IgG2B Goat IgG
AF2989 MAB2989 AF4116 AF2796 AF3087 MAB3930 AF1538 BAF1538 MAB1538 MAB2771 MAB23971 AF3764 MAB18401 AF4079
100 g 100 g 100 g 100 g 100 g 100 g 100 g 50 g 150 g 100 g 100 g 100 g 100 g 100 g
$355 $240 $355 $355 $355 $240 $355 $380 $240 $240 $240 $355 $240 $355
Cripto
Mouse
Biotin None
Cripto 1 CRISP-3 CRY1 Cryptic CSL
Human Human Human/Mouse Mouse Human
None None None None None
39

MOLECULE CTGF/CCN2 C-Terminus CTLA-4 Cubilin SPECIES Human Human Human Human Rat CXADR CXCL1/GROa/KC/CINC-1 CXCL2/GROb/MIP-2/CINC-3 Mouse Rat Mouse LABEL None None None None None None None None None None Biotin None None None Biotin None None Canine Biotin None CXCL8/IL-8 Feline None None Human Biotin None CXCL8/IL-8 NorthernLights-conjugated CXCL9/MIG Human Human Human CXCL10/IP-10/CRG-2 Mouse Human/Mouse Human Mouse Human Feline Human Mouse Human Human Human Human NL637 None None None None Biotin None None None None Biotin None None None None None None None None CLONE Polyclonal 88430 Polyclonal Polyclonal Polyclonal 81513 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 33160 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 258911 Polyclonal Polyclonal Polyclonal 6217 6217 Polyclonal 49106 Polyclonal Polyclonal Polyclonal 79018 Polyclonal Polyclonal Polyclonal Polyclonal 374606 44716 247506 51505 56811 358426 11G8 328303 HOST/TYPE Goat IgG Mouse IgG1 Goat IgG Sheep IgG Goat IgG Mouse IgG1 Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG1 Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG1 Goat IgG Goat IgG Goat IgG Mouse IgG1 Mouse IgG1 Goat IgG Mouse IgG1 Goat IgG Goat IgG Goat IgG Mouse IgG1 Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG2A Mouse IgG2B Rat IgG2B Mouse IgG2B Mouse IgG2B Mouse IgG2A Mouse IgG1 Mouse IgG1 CATALOG # AF660 MAB660 AF-386-PB AF3700 AF365 MAB3651 AF537 AF2654 AF-515-NA AF-452-NA BAF452 AF254 MAB254 AF793 BAF793 AF1116 AF1608 BAF1608 MAB1608 AF2277 AF-208-NA BAF208 MAB208 NL208V AF392 MAB392 AF-266-NA AF-466-NA BAF466 MAB350 AF801 AF470 AF976 BAF976 MAB4287 MAB172 MAB21651 MAB190 MAB699 MAB4227 MAB42273 MAB4314 SIZE 100 g 500 g 100 g 100 g 100 g 500 g 100 g 100 g 100 g 100 g 50 g 100 g 500 g 100 g 50 g 100 g 100 g 50 g 100 g 100 g 100 g 50 g 500 g 500 L 100 g 500 g 100 g 100 g 50 g 500 g 100 g 100 g 100 g 50 g 100 g 500 g 100 g 500 g 500 g 100 g 100 g 100 g PRICE $355 $325 $355 $355 $355 $325 $355 $355 $355 $355 $380 $355 $325 $355 $380 $355 $355 $380 $240 $355 $355 $380 $325 $345 $355 $325 $355 $355 $380 $325 $355 $355 $355 $380 $240 $325 $325 $325 $325 $240 $240 $240
CX3CL1/Fractalkine Chemokine Domain
CXCL5/ENA-78
Human
CXCL7/Thymus Chemokine-1
Mouse Rat
CXCL12/SDF-1 CXCL13/BLC/BCA-1
CXCL16 CXCR4 CXCR4 CXCR5 CXCR6 CXCR7/RDC-1 Cyclin D1
40

MOLECULE Cyr61/CCN1 SPECIES Mouse LABEL None None CLONE Polyclonal 466305 HOST/TYPE Sheep IgG Rat IgG2B CATALOG # AF4055 MAB4864 SIZE 100 g 100 g PRICE $355 $240
Cyr61 in Developing Embryonic Mouse Rib. Cyr61 was detected in cryostat sections of E13 mouse embryo using anti-mouse Cry61 affinity-purified polyclonal antibody (Catalog # AF4055). Tissues were stained using anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).
Cystatin A Cystatin B
Human Human Mouse
None None None
Polyclonal Polyclonal 227807
Goat IgG Goat IgG Rat IgG2A
AF1407 AF1408 MAB14091
100 g 100 g 500 g
$355 $355 $325
Cystatin B in Mouse Cerebellum. Cystatin B was detected in a frozen section of mouse cerebellum using anti-mouse Cystatin B monoclonal antibody (Catalog # MAB14091). Tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013). Tissue was stained using anti-rat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS017; brown) and counterstained with hematoxylin (blue).
Cystatin C
Human Mouse
None None None
Polyclonal Polyclonal Polyclonal Polyclonal 211515 2B5F8 LP3K LL001 BA17 Polyclonal 293904 358307 375604 Polyclonal Polyclonal 120507 120612 Polyclonal 270012 270012 Polyclonal Polyclonal 290814 290804 Polyclonal Polyclonal 115402 Polyclonal Polyclonal
Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG2B Mouse IgG2A Mouse IgG1 Mouse IgG2A Mouse IgG1 Goat IgG Mouse IgG1 Mouse IgG2A Rat IgG2A Goat IgG Goat IgG Mouse IgG2B Mouse IgG2A Goat IgG Rat IgG2A Rat IgG2A Goat IgG Goat IgG Mouse IgG2B Mouse IgG2B Goat IgG Goat IgG Mouse IgG1 Goat IgG Goat IgG
AF1196 AF1238 AF1286 BAF1286 MAB1286 MAB898 MAB3165 MAB3164 MAB3506 AF955 MAB3095 MAB4139 MAB4230 AF142 BAF142 MAB161 MAB1621 AF2378 BAM2378 MAB2378 AF2396 AF2538 MAB2538 MAB25381 AF2047 AF143 MAB143 AF1060 BAF1060
$355 $355 $355 $380 $325 $240 $240 $240 $240 $355 $240 $240 $240 $355 $380 $325 $325 $355 $380 $240 $355 $355 $240 $240 $355 $355 $325 $355 $380
Cystatin E/M
Human
Biotin None
Cytochrome c Cytokeratin 8 Cytokeratin 14 Cytokeratin 19 DAN DANCE DARC DARPP-32 aa 51-204 DcR3/TNFRSF6B DC-SIGN DC-SIGN+DC-SIGNR
Human/Equine Human Human Human Human Human Human Mouse/Rat Human Human Human
None None None None None None None None None Biotin None None None
DcTRAIL R1/TNFRSF23
Mouse
Biotin None
DDR1 DDR2 DDR2 DEC-205/CD205
Human Human Human Human Human
None None None None None None None None Biotin
Decorin Mouse
41

MOLECULE Dectin-1/CLEC7A b-Defensin 2/BD-2 Desert Hedgehog C-Terminus Desert Hedgehog N-Terminus Desmin SPECIES Human Human Mouse Mouse Human LABEL None None None None Biotin None CLONE 259931 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal HOST/TYPE Mouse IgG2B Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG CATALOG # MAB1859 AF2758 AF196 AF733 BAF733 AF3844 SIZE 100 g 100 g 100 g 100 g 50 g 100 g PRICE $240 $355 $355 $355 $380 $355
Desmin in Human Heart. Desmin was detected in paraffin-embedded human heart sections using antihuman desmin affinity-purified polyclonal antibody (Catalog # AF3844). Tissue was stained using the antigoat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Desmoglein-1 Propeptide
Human
None Biotin None None None
Polyclonal Polyclonal Polyclonal 216519 Polyclonal
Goat IgG Goat IgG Goat IgG Mouse IgG2B Goat IgG
AF944 BAF944 AF1720 MAB1720 AF3316
100 g 50 g 100 g 500 g 100 g
$355 $380 $355 $325 $355
Desmoglein-3 Dishevelled-1
Human Human
Dishevelled-1 in Human Skeletal Muscle. Dishevelled-1 was detected in a paraffin-embedded human skeletal muscle section using anti-human dishevelled-1 affinity-purified polyclonal antibody (Catalog # AF3316). The tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Dishevelled-3
Human
None None
Polyclonal Polyclonal 259631 259631 Polyclonal Polyclonal Polyclonal Polyclonal 251123 Polyclonal Polyclonal
Sheep IgG Goat IgG Rat IgG2B Rat IgG2B Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG2B Sheep IgG Sheep IgG
AF3754 AF1765 BAM1765 MAB1765 AF2435 AF1118 BAF1118 AF1376 MAB18181 AF5026 AF3970
100 g 100 g 100 g 100 g 100 g 100 g 50 g 100 g 100 g 100 g 100 g
$355 $355 $380 $240 $355 $355 $380 $355 $240 $355 $355
Dkk-1
Mouse
Biotin None
Dkk-2 Dkk-3 DLEC/CLEC4C/BDCA2
Mouse Human Human Human
None None Biotin None None None None
DLL1
Mouse/Rat Rat
DLL1 Expression in Mouse Embyronic Stomach. Delta-like 1 (DLL1) was detected in a cryostat tissue section of mouse E13.5 embryonic stomach using anti-rat DLL1 affinity-purified polyclonal antibody (Catalog # AF3970). Tissues were stained using NorthernLights-557-conjugated anti-sheep secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue).
DMC/VCC-1
Human
None
422208
Mouse IgG2B
MAB4207
100 g
$240
42

MOLECULE DMP-1 SPECIES Mouse LABEL None CLONE Polyclonal HOST/TYPE Sheep IgG CATALOG # AF4386 SIZE 100 g PRICE $355
DMP-1 in Developing Mouse Teeth. Dentin Matrix Protein 1 (DMP-1) was detected in a cryostat section of developing embryonic (E15.5) mouse teeth using anti-mouse DMP-1 affinity-purified polyclonal antibody (Catalog # AF4386). Cells were stained using NorthernLights-557 donkey anti-sheep IgG secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue).
Human DNER
None Biotin None Biotin None None
Polyclonal Polyclonal Polyclonal 298306 298306 Polyclonal
Goat IgG Goat IgG Goat IgG Rat IgG1 Rat IgG1 Sheep IgG
AF3646 BAF3646 AF2254 BAM2254 MAB2254 AF4826
100 g 50 g 100 g 100 g 100 g 100 g
$355 $380 $355 $380 $240 $355
Mouse Mouse
DOK4
Human
DOK4 in Human Kidney. Downstream of kinase 4 (DOK4) was detected in a paraffin-embedded section of human kidney using anti-human DOK4 affinitypurified polyclonal antibody (Catalog # AF4826). Tissues were stained using anti-sheep HRP-DAB detection reagents (brown) and counterstained with hematoxylin (blue).
Dopa Decarboxylase/DDC Dopamine Transporter C-Terminus Dopamine Transporter Extracellular Loop 2 DPP6 DPPA4
Human Human/Mouse/Primate Human/Primate Human Human Mouse Human
None None None None None None None None Biotin None None Biotin None None None
Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 399212
Goat IgG Rabbit IgG Rabbit IgG Goat IgG Sheep IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG2B
AF3564 PPS068 PPS069 AF2360 AF3674 AF3730 AF3125 AF3984 BAF3984 AF1180 AF954 BAF954 AF2437 AF3666 MAB36661
100 g 100 L 100 L 100 g 100 g 100 g 100 g 100 g 50 g 100 g 100 g 50 g 100 g 100 g 100 g
$355 $355 $355 $355 $355 $355 $355 $355 $380 $355 $355 $380 $355 $355 $240
DPPA5/ESG1
Mouse Human
DPPIV/CD26
Mouse Mouse Human
DR3/TNFRSF25 DSCAM Long Isoform
DSCAM in Human A172 Cells. Down Syndrome Cell Adhesion Molecule (DSCAM) was detected in human A172 glioblastoma cell line using anti-human DSCAM affinity-purified polyclonal antibody (Catalog # AF3666). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).
DSPG3
Human
None
329707
Mouse IgG1
MAB3110
100 g
$240
43

MOLECULE SPECIES Human Dtk LABEL None None Mouse Biotin None Phospho-Dynamin (S774) DYRK1A aa 438-571 E2F-1 EAR2/NR2F6 ECE-1 ECE-2 ECM-1 Human/Mouse/Rat/Bovine/ Canine/Chicken/Primate Human/Rat Human Human Human Human Human None None None None None None None CLONE 96201 Polyclonal 109646 109646 Polyclonal Polyclonal Polyclonal N2025 Polyclonal 233914 Polyclonal HOST/TYPE Mouse IgG1 Goat IgG Rat IgG1 Rat IgG1 Sheep IgG Sheep IgG Sheep IgG Mouse IgG2A Goat IgG Rat IgG2A Sheep IgG CATALOG # MAB859 AF759 BAM759 MAB759 PPS004 AF5407 AF4825 PP-N2025-00 AF1784 MAB16451 AF3937 SIZE 500 g 100 g 100 g 500 g 100 L 100 g 100 g 100 g 100 g 100 g 100 g PRICE $325 $355 $380 $325 $355 $355 $355 $380 $355 $240 $355
ECM-1 in Human Colon Cancer. Extracellular matrix protein-1 (ECM-1) was detected in paraffin-embedded sections of human colon cancer using antihuman ECM-1 affinity-purified polyclonal antibody (Catalog # AF3937). Tissue was stained using antisheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).
None Human EGF Mouse None None None None None Biotin None None None None
Polyclonal 10825 10827 Polyclonal 463004 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 406913
Goat IgG Mouse IgG1 Mouse IgG1 Goat IgG Rat IgG2A Goat IgG Goat IgG Rabbit IgG Rabbit IgG Goat IgG Rat IgG2A
AF236 MAB236 MAB636 AF2028 MAB5256 AF1280 BAF1280 AF1095 AF3394 AF2818 MAB2818
100 g 500 g 500 g 100 g 100 g 100 g 50 g 50 g 50 g 100 g 100 g
$355 $325 $325 $355 $240 $355 $380 $285 $285 $355 $240
EGF R/ErbB1 Phospho-EGF R/ErbB1 (Y1173) Phospho-EGF R/ErbB1 (Y845) EGR1
Mouse Human Human Human
EGR1 in Human Prostate Cancer. Early growth response 1 (EGR1) was detected in a paraffin-embedded human prostate cancer tissue section using antihuman EGR1 monoclonal antibody (Catalog # MAB2818). Tissues were stained using the anti-rat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS017; brown) and counterstained with hematoxylin (blue).
eIF4E ELF3 aa 375-552 EMAP-II
Human/Mouse/Rat Human/Mouse Human
None None None
299910 Polyclonal Polyclonal
Mouse IgG2B Goat IgG Goat IgG
MAB3228 AF5787 AF1910
100 g 100 g 100 g
$240 $355 $355
EMAP-II in Human Colon Cancer. Endothelial Monocyte-activation Polypeptide II (EMAP-II) was detected in a paraffin-embedded human colon cancer section using anti-human EMAP-II affinity-purified polyclonal antibody (Catalog # AF1910). Tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
44

MOLECULE EMMPRIN EMR1 SPECIES Human Mouse Human Endoglin/CD105 Mouse LABEL None None None Biotin None Biotin None Human Mouse None None None CLONE Polyclonal 521204 Polyclonal Polyclonal Polyclonal Polyclonal 209701 Polyclonal 211816 Polyclonal HOST/TYPE Goat IgG Rat IgG2A Goat IgG Goat IgG Goat IgG Goat IgG Rat IgG2A Goat IgG Mouse IgG2A Goat IgG CATALOG # AF972 MAB5580 AF1097 BAF1097 AF1320 BAF1320 MAB1320 AF1524 MAB1524 AF3534 SIZE 100 g 100 g 100 g 50 g 100 g 50 g 100 g 100 g 100 g 100 g PRICE $355 $240 $355 $380 $355 $380 $240 $355 $240 $355
Endoglycan/PODLX2
Endoglycan in Mouse Testis. Endoglycan was detected in a cryostat section of mouse testis using antimouse Endoglycan affinity-purified polyclonal antibody (Catalog # AF3534). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Endomucin Endorepellin/Perlecan
Mouse Human Human Mouse
None None None Biotin None None None None None None None Biotin None None
Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 3G10 279122 Polyclonal Polyclonal Polyclonal Polyclonal 158210 Polyclonal Polyclonal 304519 Polyclonal Polyclonal
Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Sheep IgG Rat IgG2B Mouse IgG2B Sheep IgG Sheep IgG Goat IgG Goat IgG Mouse IgG2A Goat IgG Goat IgG Mouse IgG2B Goat IgG Goat IgG
AF4666 AF2364 AF1098 BAF1098 AF570 AF4496 MAB3440 MAB2600 AF5169 AF5160 AF1052 BAF1052 MAB960 AF2245 BAF2245 MAB22451 AF2749 BAF2749
100 g 100 g 100 g 50 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 50 g 500 g 100 g 50 g 100 g 100 g 50 g
$355 $355 $355 $380 $355 $355 $240 $240 $355 $355 $355 $380 $325 $355 $380 $240 $355 $380
Endostatin
EDNRB/Endothelin R Type B Endothelin-1 C-Terminus, aa 53-73 (EDN1) and 15-22 (Mature) Engrailed-2 Enolase 2 ENPP-7/Alk-SMase EN-RAGE/S100A12 EpCAM/TROP-1
Human Human Human Human/Mouse Mouse Human Human
Human EPCR Mouse
Biotin None None Biotin
EPCR in Normal Human Skin. Endothelial Protein C Receptor (EPCR) was detected in a paraffin-embedded human skin section using anti-human EPCR monoclonal antibody (Catalog # MAB22451). The tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).
Human EphA1 Mouse
None None None
Goat IgG Goat IgG Rat IgG2A
AF638 AF3034 MAB30341
100 g 100 g 100 g
$355 $355 $240
45

MOLECULE SPECIES Human EphA2 Mouse EphA3 EphA4 EphA5 Mouse Mouse Rat LABEL None None None Biotin None None None None CLONE Polyclonal 371805 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 86731 HOST/TYPE Goat IgG Mouse IgG2A Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG1 CATALOG # AF3035 MAB3035 AF639 BAF639 AF640 AF641 AF541 MAB541 SIZE 100 g 100 g 100 g 50 g 100 g 100 g 100 g 500 g PRICE $355 $240 $355 $380 $355 $355 $355 $325
EphA5 in Human U-118 MG Cells. EphA5 was detected in human U-118 MG glioblastoma cell line using anti-rat EphA5 monoclonal antibody (Catalog # MAB541). Cells were stained using NorthernLights-557 donkey anti-mouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).
EphB1
Rat
None None None
Polyclonal 88512 Polyclonal 512012 512001
Goat IgG Mouse IgG1 Goat IgG Rat IgG2A Rat IgG2A
AF542 MAB5421 AF467 MAB467 MAB4671
100 g 100 g 100 g 100 g 100 g
$355 $240 $355 $240 $240
EphB2
Mouse
None None
EphB2 in Embryonic Mouse Brain. EphB2 was detected in a frozen section of E15 mouse brain using anti-mouse EphB2 affinity-purified polyclonal antibody (Catalog # AF467). Tissues were stained using NorthernLights-557 secondary antibody (Catalog # NL001; red) and counterstained (green).
EphB3
Mouse Human
None None None Biotin None
Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal
Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG
AF432 AF3038 AF446 BAF446 AF603
100 g 100 g 100 g 50 g 100 g
$355 $355 $355 $380 $355
EphB4
Mouse Mouse
Ephrin-A2
Ephrin-A2 in Rat Embryonic Neurons. Ephrin-A2 was detected in rat embryonic hippocampal neurons using anti-mouse Ephrin-A2 affinity-purified polyclonal antibody (Catalog # AF603; green). Astrocytes were stained using an anti-GFAP antibody (red).
Ephrin-A5 Ephrin-B1 Ephrin-B2 Ephrin-B3 Epigen
Human Mouse Mouse Human Mouse
Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal
Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG
AF3743 AF473 AF496 BAF496 AF395 AF1127
100 g 100 g 100 g 50 g 100 g 100 g
$355 $355 $355 $380 $355 $355
46

MOLECULE Epimorphin/Syntaxin 2 SPECIES Mouse LABEL None None CLONE Polyclonal 338930 HOST/TYPE Goat IgG Rat IgG2A CATALOG # AF2568 MAB2568 SIZE 100 g 100 g PRICE $355 $240
Epimorphin/Syntaxin 2 in Mouse Eye. Epimorphin was detected in frozen embryonic mouse eye cup sections using anti-mouse Epimorphin monoclonal antibody (Catalog # MAB2568). Tissue was stained using the anti-rat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS017; brown) and counterstained with hematoxylin (blue).
Epiregulin
Human
None None
Polyclonal Polyclonal Polyclonal 191924 416711
Goat IgG Goat IgG Goat IgG Mouse IgG2B Mouse IgG2B
AF1195 AF1129 BAF1129 MAB1129 MAB11291
100 g 100 g 50 g 500 g 100 g
$355 $355 $380 $325 $240
ErbB2/Her2
Human
Biotin None None
Phospho-ErbB2 in Human Breast Cancer. Phospho-ErbB2 was detected in paraffin-embedded human breast cancer tissue sections using anti-human phospho-ErbB2 affinity-purified polyclonal antibody (Catalog # AF1768). Tissues were stained using antirabbit HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS005; brown) and counterstained with hematoxylin (blue)
Phospho-ErbB2/Her2 (Y1196) Phospho-ErbB2/Her2 (Y1248)
Polyclonal Polyclonal 66219 526806 Polyclonal 216703
Rabbit IgG Rabbit IgG Mouse IgG1 Mouse IgG2B Sheep IgG Mouse IgG1
AF4438 AF1768 MAB348 MAB3483 AF4518 MAB1576
100 g 50 g 500 g 100 g 100 g 100 g
$285 $285 $325 $240 $355 $240
ErbB3/Her3
ERK1/ERK2
Human/Mouse/Rat
Phosphorylated ERK1/ERK2 Expression in Rat Brain Cortex. Phosphorylated ERK1/ERK2 was detected in cryostat tissue sections of rat brain cortex using anti-human/mouse/rat phospho-ERK1/ERK2 affinity-purified polyclonal antibody (Catalog # AF1018). Tissue was stained using the anti-rabbit HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS005; brown) and counterstained with hematoxylin (blue).
Phospho-ERK1/ERK2 (ERK1 T202/Y204, ERK2 T185/Y187) ERK1 ERK1 aa 1-28
Human/Mouse/Rat Human/Mouse/Rat Human
None None None None
Polyclonal Polyclonal 250603 Polyclonal Polyclonal 191801 335102 355810 Polyclonal H5844 H5844 H6705 H6812
Rabbit IgG Rabbit IgG Mouse IgG2A Rabbit IgG Goat IgG Mouse IgG2A Mouse IgG2B Rat IgG2A Goat IgG Mouse IgG2A Mouse IgG2A Mouse IgG2A Mouse IgG2A
AF1018 AF1575 MAB1940 AF1230 AF12301 MAB1230 MAB3196 MAB3914 AF2848 PP-H5844-00 PP-H5844-10 PP-H6705-00 PP-H6812-00
100 g 50 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g
$285 $285 $240 $285 $355 $240 $240 $240 $355 $380 $380 $380 $380
ERK2
Human/Mouse/Rat
None None
ERK3 aa 344-721 ERK4 aa 1-126 ERK5/BMK1 aa 1-432 ERRa/NR3B1 ERRb/NR3B2 ERRg/NR3B3
Human Human/Mouse/Rat Human/Mouse Human Human Human
47

MOLECULE Erythropoietin Erythropoietin R SPECIES Human Mouse LABEL None None Biotin None Human ESAM Mouse None None None None CLONE Polyclonal Polyclonal Polyclonal Polyclonal 408519 408522 Polyclonal 340236 HOST/TYPE Total Rabbit IgG Goat IgG Goat IgG Goat IgG Mouse IgG2B Mouse IgG2B Goat IgG Rat IgG2A CATALOG # AB-286-NA AF1390 BAF1390 AF2688 MAB4204 MAB42041 AF2827 MAB28271 SIZE 1 mg 100 g 50 g 100 g 100 g 100 g 100 g 100 g PRICE $355 $355 $380 $355 $240 $240 $355 $240
ESAM in Human Kidney. Endothelial Cell Adhesion Molecule (ESAM) was detected in paraffin-embedded human kidney sections using anti-human ESAM monoclonal antibody (Catalog # MAB42041). Tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013) and stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown). Tissue was counterstained with hematoxylin (blue).
ESGP ERa/NR3A1 EZH2
Mouse Human Human/Mouse Human
None None None None None None None None None None None
Polyclonal Polyclonal H4624 Polyclonal Polyclonal 328607 Polyclonal 220119 Polyclonal Polyclonal Polyclonal Polyclonal 242305 Polyclonal Polyclonal Polyclonal 258417
Sheep IgG Sheep IgG Mouse IgG2A Goat IgG Goat IgG Mouse IgG2A Goat IgG Mouse IgG2B Goat IgG Goat IgG Sheep IgG Sheep IgG Rat IgG2A Goat IgG Goat IgG Goat IgG Rat IgG2A
AF4580 AF5715 PP-H4624-00 AF4767 AF2964 MAB2964 AF1565 MAB1565 AF3078 AF1486 AF1678 BAF1678 MAB1678 AF3150 AF1443 BAF1443 MAB1443
$355 $355 $380 $355 $355 $240 $355 $240 $355 $355 $355 $380 $240 $355 $355 $380 $240
FABP1 Rat Human Rat
FABP2
FABP3
Human
Biotin None
Human FABP4
None None
Mouse
Biotin None
FABP4 in Mouse Mesenchymal Stem Cells. Fatty Acid Binding Protein 4 (FABP4) was detected in mouse mesenchymal stem cells using anti-mouse FABP4 affinity-purified polyclonal antibody (Catalog # AF1443). Cells were stained using anti-goat NorthernLights-557 (Catalog # NL001; red) and counterstained with DAPI (blue).
FABP5FABP5
Human Mouse
None None None Biotin None Biotin None None None
Polyclonal 311215 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 324926
Goat IgG Rat IgG2A Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Rat IgG2A
AF3077 MAB3077 AF1476 BAF1476 AF3880 BAF3880 AF3166 AF2750 MAB2750
100 g 100 g 100 g 50 g 100 g 50 g 100 g 100 g 100 g
$355 $240 $355 $380 $355 $380 $355 $355 $240
FABP5 FABP6 FABP7 FABP9
Mouse Human Human Mouse
48

MOLECULE FAK aa 213-412 SPECIES Human Human/Mouse/Rat LABEL None None CLONE 495919 Polyclonal HOST/TYPE Mouse IgG2B Sheep IgG CATALOG # MAB4467 AF4467 SIZE 100 g 100 g PRICE $240 $355
FAK in Human Hippocampus. Focal adhesion kinase 1 (FAK) was detected in paraffin-embedded human brain hippocampus sections using anti-human/ mouse/rat FAK affinity-purified polyclonal antibody (Catalog # AF4467). The tissue was stained using the anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).
Fibroblast Activation Protein a/FAP
Human Feline
None None Biotin None None None None None None None None None None Biotin None None Biotin None None None
Polyclonal Polyclonal Polyclonal DX2 Polyclonal Polyclonal Polyclonal Polyclonal 252029 308420 10.1 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 254625 Polyclonal Polyclonal Polyclonal 221903 299026 Polyclonal 47109 Polyclonal Polyclonal Polyclonal 121630 117601 Polyclonal 272317 461804 283507 Polyclonal
Sheep IgG Goat IgG Goat IgG Mouse IgG1 Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG2B Mouse IgG2B Mouse IgG1 Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Total Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG2A Goat IgG Goat IgG Goat IgG Mouse IgG1 Mouse IgG1 Goat IgG Mouse IgG1 Goat IgG Goat IgG Goat IgG Mouse IgG2B Mouse IgG1 Goat IgG Rat IgG2A Mouse IgG2B Rat IgG2A Goat IgG
AF3715 AF2267 BAF2267 MAB142 AF435 AF2159 AF126 AF1858 MAB1858 MAB3304 MAB1257 AF1460 AF1330 BAF1330 AF1960 AF2426 BAF2426 AF1184 AF232 AB-233-NA AF-233-NA BAF233 AF1206 MAB1206 AF235 BAF235 AF-237-NA MAB2371 MAB1957 AF-251-NA MAB323 AF-423-NA AF-273-NA AF345 MAB319 MAB969 AF2547 MAB2547 MAB25372 MAB26291 AF1413
100 g 100 g 50 g 500 g 100 g 100 g 100 g 100 g 500 g 100 g 500 g 100 g 100 g 50 g 100 g 100 g 50 g 100 g 100 g 1 mg 100 g 50 g 100 g 500 g 100 g 50 g 100 g 500 g 100 g 250 g 500 g 100 g 100 g 100 g 500 g 500 g 100 g 100 g 100 g 100 g 100 g
$355 $355 $380 $325 $355 $355 $355 $355 $325 $240 $325 $355 $355 $380 $355 $355 $380 $355 $355 $355 $355 $380 $355 $325 $355 $380 $355 $325 $240 $315 $325 $355 $355 $355 $325 $325 $355 $240 $240 $240 $355
Fas/TNFRSF6/CD95
Human Mouse Rat Human
Fas Ligand/TNFSF6
Rat Human Human Mouse Human Mouse Human Human Human
FATP1 Fcg RI/CD64 Fcg RII/RIII (CD32/CD16) Fcg RIIB/CD32b Fcg RIIIA/B (CD16) FCRL4/FcRH4 Fetuin A/AHSG FGF acidic
FGF basic
Human
None Biotin
FGF-3
Human
None None None Biotin None None None None None None None None None None None None None None None
FGF-4
Human
FGF-5
Human Canine Human Human/Mouse Mouse Human Human Human Human Human Human Mouse Rat
KGF/FGF-7 FGF-8 FGF-8b Isoform FGF-9 FGF-10 FGF-17 FGF-19 FGF-20 FGF-21 FGF-23 FGF-BP
49

MOLECULE FGF R2 FGF R3 (IIIc) SPECIES Human Human Human FGF R4 Mouse Mouse Human Human/Rat LABEL None None None None Biotin None None None None Fibronectin Human None None
Fibronectin in Human WS-1 Cells. Fibronectin was detected in WS-1 human dermal fibroblasts using anti-human Fibronectin monoclonal antibody (Catalog # MAB19181). Cells were stained using NorthernLights-557 donkey anti-mouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).
CLONE 98739 136312 240929 Polyclonal Polyclonal Polyclonal 349331 Polyclonal Polyclonal P1H11 P1F11
HOST/TYPE Mouse IgG1 Mouse IgG1 Rat IgG2A Goat IgG Goat IgG Goat IgG Mouse IgG2A Goat IgG Sheep IgG Mouse IgG1 Mouse IgM
CATALOG # MAB6843 MAB7662 MAB6852 AF2265 BAF2265 AF1899 MAB32071 AF3207 AF1918 MAB1918 MAB19181
SIZE 500 g 500 g 100 g 100 g 50 g 100 g 100 g 100 g 100 g 100 g 100 g
PRICE $325 $325 $240 $355 $380 $355 $240 $355 $355 $240 $240
FGF R5/FGFRL1 Fgr Fgr aa 1-82
FKBP38 aa 1-326 FKBP51
Human/Mouse/Rat Human/Mouse/Rat
None None
Goat IgG Goat IgG
AF3580 AF4094
100 g 100 g
$355 $355
FKBP51 in Human Prostate. FK506 Binding Protein, 51 kDa molecular weight (FKBP51), was detected in paraffin-embedded human prostate sections using anti-human/mouse/rat FKBP51 affinity-purified polyclonal antibody (Catalog # AF4094). Tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
FKBP52 Follistatin-related Gene Protein/FLRG Flt-3/Flk-2 Phospho-Flt-3/Flk-2 (Y591) Flt-3 Ligand Follistatin
Human Mouse Mouse Human Feline Human Human Mouse/Rat Human Human Human
None None Biotin None None None None None None None None None None Biotin None None
425123 Polyclonal Polyclonal 113308 Polyclonal Polyclonal Polyclonal 85918 Polyclonal 228208 548908 Polyclonal Polyclonal Polyclonal Polyclonal 407003
Rat IgG1 Goat IgG Goat IgG Rat IgG2A Rabbit IgG Goat IgG Goat IgG Mouse IgG2A Goat IgG Rat IgG2B Mouse IgG1 Goat IgG Sheep IgG Sheep IgG Goat IgG Rat IgG2B
MAB4095 AF1255 BAF1255 MAB7681 AF368 AF2467 AF669 MAB669 AF1694 MAB17381 MAB5646 AF2214 AF4248 BAF4248 AF3619 MAB3619
$240 $355 $380 $240 $285 $355 $355 $325 $355 $240 $240 $355 $355 $380 $355 $240
Follistatin-like 1/FSL1 FOLR1 FosB/G0S3 FoxH1
FoxJ1
Human
50

MOLECULE FoxM1 SPECIES Human LABEL None CLONE Polyclonal HOST/TYPE Sheep IgG CATALOG # AF3975 SIZE 100 g PRICE $355
FoxM1 in Human U2OS Cells. FoxM1 was detected in human osteosarcoma U2OS cells using antihuman FoxM1 affinity-purified polyclonal antibody (Catalog # AF3975). Cells were stained using NorthernLights-557 donkey anti-sheep IgG secondary antibody (Catalog # NL010; red) and counterstained (green).
FoxP2 FoxP3
Human/Mouse Human
None None
Sheep IgG Goat IgG
AF5647 AF3240
100 g 100 g
$355 $355
FoxP3 in Human Tonsil. FoxP3 was detected in paraffin-embedded human tonsil sections using antihuman FoxP3 affinity-purified polyclonal antibody (Catalog # AF3240). Tissue was stained using antigoat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Human/Mouse Frizzled-1 Mouse
Biotin None Biotin
Rat IgG2A Goat IgG Goat IgG
BAM11201 AF1120 BAF1120
100 g 100 g 50 g
$240 $355 $380
Frizzled-1 Expression in Mouse Intestine. Frizzled-1 was detected in a cryostat tissue section of embryonic mouse intestine (E13) using anti-mouse Frizzled-1 affinity-purified polyclonal antibody (Catalog # AF1120). Tissues were stained using the antigoat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Human/Mouse Frizzled-3 Mouse
None None None Biotin Biotin
169310 169310 Polyclonal 169310 145901 145901 145901 Polyclonal Polyclonal 151143 151143 Polyclonal Polyclonal Polyclonal
Rat IgG2A Rat IgG2A Goat IgG Rat IgG2A Rat IgG2A Rat IgG2A Rat IgG2A Goat IgG Goat IgG Rat IgG2A Rat IgG2A Goat IgG Goat IgG Goat IgG
MAB1001-050 MAB1001-100 AF1001 BAM1001 BAM194 MAB194-050 MAB194-100 AF194 AF1526 MAB1981-050 MAB1981-100 AF198 AF112 AF2440
50 g 100 g 100 g 100 g 100 g 50 g 100 g 100 g 100 g 50 g 100 g 100 g 100 g 100 g
$150 $240 $355 $295 $240 $150 $240 $355 $355 $150 $240 $355 $355 $355
Frizzled-4
Human/Mouse
None None
Mouse Frizzled-6 Mouse Human/Mouse Mouse Frizzled-8 Frizzled-9 Mouse Mouse
Frizzled-7
51

MOLECULE Frk aa 1-107 SPECIES Human Human/Mouse/Rat LABEL None None CLONE 393812 Polyclonal HOST/TYPE Rat IgG1 Goat IgG CATALOG # MAB3766 AF3766 SIZE 100 g 100 g PRICE $240 $355
Frk in Human Breast Cancer. Fyn-related kinase (Frk) was detected in paraffin-embedded human breast cancer sections using anti-human Frk monoclonal antibody (Catalog # MAB3766). Tissue was subjected to antigen retrieval using Antigen Retrieval Kit (Catalog # CTS013; brown). Tissue was stained using anti-rat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS017; brown) and counterstained with hematoxylin (blue).
FRS2 Fucosyltransferase 8/FUT8 Furin FXR/NR1H4 Fyn G9a/EHMT2 GABAA R d N-Terminus GABAB R2 N-Terminus GAD1/GAD67 aa 2-97 GAD2/GAD65 aa 2-150
Human Human Human Human Human Human/Mouse Mouse/Rat Rat Human Human Human
None None None None None None None None None None None None None
Polyclonal Polyclonal 222728 A9033A Polyclonal A8620A Polyclonal Polyclonal Polyclonal 252910 Polyclonal Polyclonal Polyclonal Polyclonal 201002 201002 Polyclonal 194804 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 212923 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 288518 289802 234732 Polyclonal Polyclonal 291106 Polyclonal Polyclonal
Sheep IgG Sheep IgG Mouse IgG1 Mouse IgG2A Goat IgG Mouse IgG2A Rabbit IgG Goat IgG Goat IgG Mouse IgG1 Goat IgG Goat IgG Goat IgG Goat IgG Rat IgG2B Rat IgG2B Goat IgG Mouse IgG2B Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Rat IgG2A Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG2B Mouse IgG2B Rat IgG2B Goat IgG Goat IgG Mouse IgG1 Goat IgG Goat IgG
AF4069 AF5768 MAB15031 PP-A9033A-00 AF3574 PP-A8620A-00 PPS090 AF1188 AF2086 MAB2086 AF2247 AF1152 AF1245 BAF1245 BAM12451 MAB12451 AF1154 MAB1154 AF1197 BAF1197 AF1227 AF2128 AF1339 BAF1339 AF1304 MAB1304 AF1305 AF2045 BAF2045 AF3535 AF2644 AF885 MAB20701 MAB21363 MAB1779 AF2046 BAF2046 MAB26051 AF2605 BAF2605
100 g 100 g 500 g 100 g 100 g 100 g 100 L 100 g 100 g 100 g 100 g 100 g 100 g 50 g 250 g 500 g 100 g 500 g 100 g 50 g 100 g 100 g 100 g 50 g 100 g 500 g 100 g 100 g 50 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 50 g 100 g 100 g 50 g
$355 $355 $325 $380 $355 $380 $355 $355 $355 $240 $355 $355 $355 $380 $380 $325 $355 $325 $355 $380 $355 $355 $355 $380 $355 $325 $355 $355 $380 $355 $355 $355 $240 $240 $240 $355 $380 $240 $355 $380
Galectin-1
Mouse
Biotin Biotin None
Human Galectin-3 Mouse Human Mouse Human Galectin-7 Mouse Galectin-8 Galectin-9 Galectin-9 Short Isoform Gas1 Gas6 GASP-1/WFIKKNRP GASP-2/WFIKKN GATA-1 GATA-2 GATA-3 GATA-3 aa 135-258 Human Human Mouse Mouse Human Human Human Human Human Human Human
None None None Biotin None None None Biotin None None None None Biotin None None None None None None None Biotin None None Biotin
Galectin-4
52

MOLECULE GATA-4 SPECIES Human LABEL None Biotin CLONE Polyclonal Polyclonal HOST/TYPE Goat IgG Goat IgG CATALOG # AF2606 BAF2606 SIZE 100 g 50 g PRICE $355 $380
GATA-4 in Differentiated Human Embryonic Stem Cells. GATA-4 was detected in differentiated human Embryonic Stem cells using anti-human GATA-4 polyclonal antibody (Catalog # AF2606; green). The cells were counterstained with DAPI (blue). Courtesy of Dr. Frank Solder from the National Institute of Neurological Disorders and Stroke & Stem Cell Unit at NIH.
GATA-5
Human
None None
Polyclonal Polyclonal Polyclonal 222228 Polyclonal Polyclonal Polyclonal Polyclonal 90509 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 273807
Goat IgG Goat IgG Goat IgG Mouse IgG1 Goat IgG Goat IgG Goat IgG Goat IgG Rat IgG2A Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Sheep IgG Sheep IgG Mouse IgG1
AF2170 AF1700 BAF1700 MAB1700 AF3180 AF958 AF853 BAF853 BAM853 AF788 BAF788 AF739 BAF739 AF-212-NA AF2594 BAF2594 MAB2594
$355 $355 $380 $240 $355 $355 $355 $380 $380 $355 $380 $355 $380 $355 $355 $380 $240
GATA-6
Human
Biotin None
GCNF/NR6A1 GDF-3
Human Mouse
None None None
GDF-5
Mouse
Biotin Biotin
GDF-8/Myostatin
Mouse
None Biotin None Biotin None None
GDF-9 GDNF
Mouse Human/Rat
GFAP
Human
Biotin None
GFAP in Rat Astrocytes. Glial fibrillary acidic protein (GFAP) was detected in astrocytes in differentiating cultures of rat cortical stem cells using antihuman GFAP affinity-purified polyclonal antibody (Catalog # AF2594). The cells were stained using NorthernLights-557 anti-sheep secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue).
GFI-1
Human Human
None None None
Polyclonal Polyclonal Polyclonal Polyclonal 81401
Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG2B
AF3540 AF714 AF560 BAF560 MAB560
100 g 100 g 100 g 50 g 500 g
$355 $355 $355 $380 $325
GFRa-1/GDNF Ra-1
Rat
Biotin None
GFRa-1 and GDNF Expression in Rat Dorsal Root Ganglia. GFRa-1 and GDNF were detected in rat dorsal root ganglia using biotinylated anti-rat GFRa-1 affinity-purified polyclonal antibody (Catalog # BAF560; red) and anti-human GDNF affinitypurified polyclonal antibody (Catalog # AF-212-NA; green).
GFRa-2/GDNF Ra-2
Human Mouse
None None
Goat IgG Goat IgG
AF613 AF429
100 g 100 g
$355 $355
53

MOLECULE SPECIES Human GFRa-3/GDNF Ra-3 Mouse LABEL None None Biotin CLONE Polyclonal Polyclonal Polyclonal HOST/TYPE Goat IgG Goat IgG Goat IgG CATALOG # AF670 AF2645 BAF2645 SIZE 100 g 100 g 50 g PRICE $355 $355 $380
GFRa-3 in Mouse Embryonic Dorsal Root Ganglion (DRG). Detection of Glial cell line-derived Factor Receptor alpha 3 (GFRa-3) in a cryostat section of E15 DRG mouse using anti-mouse GFRa-3 affinity-purified polyclonal antibody (Catalog # AF2645). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (brown; Catalog # CTS008 and counterstained with hematoxylin (blue).
None GFRa-4/GDNF Ra-4 Mouse Biotin None GITR/TNFRSF18 GLI-1 GLI-1 aa 1-234 GLI-1 aa 1-237 GLI-2 GLI-2 aa 1-416 GLI-3 aa 1-479 GLP/EHMT1 GLP-1R GLP-2R Glucagon Human Human/Mouse Human Human/Mouse Human Mouse Human/Mouse Human/Mouse Human Human Human/Mouse None None None None None Biotin None None None None None None
Polyclonal Polyclonal 171217 Polyclonal 388516 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal B0422 419208 413801 181402
Goat IgG Goat IgG Rat IgG2A Goat IgG Rat IgG2A Goat IgG Goat IgG Sheep IgG Sheep IgG Goat IgG Goat IgG Mouse IgG2A Mouse IgG2A Mouse IgG2A Mouse IgG2A
AF1677 BAF1677 MAB1677 AF689 MAB3324 AF3324 AF3455 AF3526 BAF3526 AF3635 AF3690 PP-B0422-00 MAB28141 MAB4285 MAB1249
$355 $380 $325 $355 $240 $355 $355 $355 $380 $355 $355 $380 $240 $240 $240
Glucagon in bTC6 Cells. Glucagon expression was detected in bTC6 mouse insulinoma cells using antihuman/mouse Glucagon monoclonal antibody (Catalog # MAB1249). The cells were stained using NorthernLights-557 donkey anti-mouse IgG secondary antibody ( Catalog # NL007; red) and counterstained with DAPI (blue).
Glucagon NorthernLights-conjugated Glut1
Human/Mouse Human Human
NL557 None None Biotin None Biotin None Biotin None Biotin None
181402 202915 199017 205115 205115 202017 202017 1F8 1F8 195205 195205
Mouse IgG2A Mouse IgG2B Mouse IgG2A Rat IgG2B Rat IgG2B Mouse IgG2B Mouse IgG2B Mouse IgG1 Mouse IgG1 Mouse IgG2A Mouse IgG2A
NL1249R MAB1418 MAB1414 BAM1440 MAB1440 BAM1415 MAB1415 BAM1262 MAB1262 BAM1349 MAB1349
500 L 200 g 200 g 200 g 200 g 200 g 200 g 250 g 200 g 200 g 200 g
$345 $270 $270 $380 $270 $315 $270 $380 $270 $380 $270
Glut2
Mouse
Glut3
Human
Glut4
Rat
Glut5
Human
54

MOLECULE Glutathione Peroxidase 1 SPECIES Human/Mouse/Rat LABEL None CLONE Polyclonal HOST/TYPE Goat IgG CATALOG # AF3798 SIZE 100 g PRICE $355
GPX1 in Rat Liver. Glutathione Peroxidase 1 (GPX1) was detected in a cryostat section of rat liver using anti-human/mouse/rat GPX1 affinity-purified polyclonal antibody (Catalog # AF3798). The tissue was stained using the anti-goat Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Glutathione Peroxidase 3 aa 74-226
Human/Mouse/Rat
None
Polyclonal
Goat IgG
AF4199
100 g
$355
GPX3 in Human Kidney. Glutathione Peroxidase 3 (GPX3) was detected in paraffin-embedded human kidney sections using anti-human GPX3 affinity-purified polyclonal antibody (Catalog # AF4199). Tissue was stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Glycine R Pan Specific Glycophorin A Glypican 1
Rat Human Human Human
None Biotin None None None None
Polyclonal R10 R10 Polyclonal Polyclonal Polyclonal Polyclonal 300736 Polyclonal Polyclonal Polyclonal Polyclonal 3209 Polyclonal Polyclonal 125623 125623
Rabbit IgG Mouse IgG1 Mouse IgG1 Goat IgG Goat IgG Goat IgG Goat IgG Rat IgG2A Sheep IgG Goat IgG Goat IgG Goat IgG Mouse IgG1 Goat IgG Goat IgG Rat IgG2A Rat IgG2A
PPS009 BAM12281 MAB1228 AF4519 AF2304 AF2355 BAF2355 MAB2355 AF2119 AF1053 AF1546 BAF1546 MAB215 AF1276 AF4086 BAM4681 MAB4681
$355 $380 $325 $355 $355 $355 $380 $240 $355 $355 $355 $380 $325 $355 $355 $240 $240
Glypican 2
Mouse
Biotin None
Glypican 3 Glypican 6
Human Mouse Canine Human
None None None Biotin None None None Biotin None
GM-CSF
GMF-b Goosecoid gp130
Human Human Mouse
gp130 in Mouse Splenocytes. gp130 was detected in mouse splenocytes using anti-mouse gp130 monoclonal antibody (Catalog # MAB4681; red). Cells were counterstained with DAPI (blue).
GPR34
Human
None
419859
Mouse IgG2A
MAB4617
100 g
$240
55

MOLECULE GPR50 SPECIES Human LABEL None CLONE 461129 HOST/TYPE Mouse IgG2A CATALOG # MAB4645 SIZE 100 g PRICE $240
GPR50 in Human Glioblastoma Cells. G proteincoupled receptor 50 (GPR50) was detected in human A172 glioblastoma cell line using anti-human GPR50 monoclonal antibody (Catalog # MAB4645). Cells were stained using NorthernLights-557 donkey antimouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).
GPRC5A GR/NR3C1
Human Human
481906 H8004 H8031 RB6-8C5 RB6-8C5 356412
Mouse IgG2A Mouse IgG2A Mouse IgG2A Rat IgG2B Rat IgG2B Mouse IgG2B
MAB5239 PP-H8004-00 PP-H8031-00 BAM1037 MAB1037 MAB29051
100 g 100 g 100 g 250 g 500 g 100 g
$240 $380 $380 $380 $325 $240
Gr-1/Ly-6G Granzyme A
Mouse Human
Granzyme A in Human Peripheral Blood Mononuclear Cells. Granzyme A was detected in human peripheral blood mononuclear cells using antihuman Granzyme A monoclonal antibody (Catalog # MAB29051). Cells were stained using donkey antimouse NorthernLights-557 secondary antibody (Catalog # NL007; red) and counterstained green.
Human Granzyme B Mouse Mouse Human Human
None None Biotin None None Biotin None
351927 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal
Mouse IgG2A Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG
MAB2906 AF1865 BAF1865 AF1365 AF1377 BAF1377 AF3846
100 g 100 g 50 g 100 g 100 g 50 g 100 g
$240 $355 $380 $355 $355 $380 $355
Granzyme D Granzyme H GRB2
GRB2 in Human Breast Cancer. Growth Factor Receptor Bound Protein 2 (GRB2) was detected in paraffin-embedded human breast cancer sections using anti-human/mouse/rat GRB2 affinity-purified polyclonal antibody (Catalog # AF3846). Tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013) and stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown). Tissue was counterstained with hematoxylin (blue).
Gremlin GRK2 aa 468-689
Mouse Human/Mouse/Rat Human
None Biotin None None Biotin None
Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 222517
Goat IgG Goat IgG Sheep IgG Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG2B
AF956 BAF956 AF4339 AF1067 BAF1067 AF1566 BAF1566 MAB15661
100 g 50 g 100 g 100 g 50 g 100 g 50 g 500 g
$355 $380 $355 $355 $380 $355 $380 $325
Growth Hormone Rat
Biotin None
56

MOLECULE SPECIES Human Growth Hormone R/GHR LABEL None Biotin None Biotin None None CLONE Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 419605 HOST/TYPE Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG1 CATALOG # AF1210 BAF1210 AF1360 BAF1360 AF1211 MAB35841 SIZE 100 g 50 g 100 g 50 g 100 g 100 g PRICE $355 $380 $355 $380 $355 $240
Mouse Rat
GRP75/HSPA9B
Human
GRP75 in Human Fibroblastic Meningioma. Glucose Regulated 75 kDa Protein (GRP75) was detected in a paraffin-embedded human fibroblastic meningioma section using anti-human GRP75 monoclonal antibody (Catalog # MAB35841). Tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).
GRP75/HSPA9B aa 542-679 GSK-3a aa 90-483 Phospho-GSK-3a (S21)
Human/Mouse/Rat Human Human/Mouse/Rat
None None None
Goat IgG Mouse IgG2B Rabbit IgG
AF3584 MAB2986 AF4125
100 g 100 g 100 g
$355 $240 $285
Phosphorylated GSK-3a in Human Thymus. Phosphorylated Glycogen Synthase kinase-3a (GSK-3a) was detected in paraffin-embedded human thymus tissue sections using anti-human/mouse/rat Phospho-GSK-3a (S27) affinity-purified polyclonal antibody (Catalog # AF4125). Tissues were stained using anti-rabbit HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS005; brown) and counterstained with hematoxylin (blue).
Phospho-GSK-3a/b (S21/S9) GSK-3b HAI-1 aa 30-433
Human/Mouse/Rat Human Mouse
None None None Biotin None Biotin None Biotin None None Biotin None None None None None None Biotin None None None None None
Polyclonal 272535 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 456738 456723 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal
Rabbit IgG Rat IgG2B Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG2A Mouse IgG2B Goat IgG Goat IgG Goat IgG Sheep IgG Goat IgG Goat IgG Goat IgG
AF1590 MAB25061 AF1141 BAF1141 AF1048 BAF1048 AF1107 BAF1107 AF5708 AF3168 BAF3168 AF3876 AF4085 AF-259-NA MAB5246 MAB52461 AF1105 BAF1105 AF3317 AF3600 AF-294-NA AF2207 AF1514
100 g 100 g 100 g 50 g 100 g 50 g 100 g 50 g 100 g 100 g 50 g 100 g 100 g 250 g 100 g 100 g 100 g 50 g 100 g 100 g 100 g 100 g 100 g
$285 $240 $355 $380 $355 $380 $355 $380 $355 $355 $380 $355 $355 $355 $240 $240 $355 $380 $355 $355 $355 $355 $355
HAI-1 Ectodomain
Human
HAI-2 Ectodomain Hairless HAND1 HAND2 HAPLN4 HB-EGF HCRTR2
Mouse Human Human Human/Mouse Human Human Human
Hepassocin HES-1 aa 93-280 HES-4 aa 94-221 HGF HGF Activator
Rat Human Human Human Mouse Human
57

MOLECULE SPECIES Human HGF R/c-MET Mouse Phospho-HGF R/c-MET (Y1003) Phospho-HGF R/c-MET (Y1234/Y1235) Phospho-HGF R/c-MET (Y1349) HIF-1a aa 575-826 HIF-2a aa 542-874 Phospho-Histone H2AX (S139) HMGA2 HNF-3b/FoxA2 aa 242-457 Human Human Human Human/Mouse Mouse Human Human Human LABEL None None None Biotin None None None None None None None None CLONE Polyclonal 95308 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 241812 Polyclonal Polyclonal Polyclonal Polyclonal HOST/TYPE Goat IgG Mouse IgG1 Goat IgG Goat IgG Rabbit IgG Rabbit IgG Rabbit IgG Mouse IgG1 Goat IgG Rabbit IgG Goat IgG Goat IgG CATALOG # AF276 MAB3583 AF527 BAF527 AF4059 AF2480 AF3950 MAB1935 AF2997 AF2288 AF3184 AF2400 SIZE 100 g 100 g 100 g 50 g 100 g 50 g 50 g 100 g 100 g 50 g 100 g 100 g PRICE $355 $240 $355 $380 $285 $285 $285 $240 $355 $285 $355 $355
HNF-3b in Human BG01V Cells. Hepatic Nuclear Factor 3 b (HNF-3b) was detected in endoderm differentiated human BG01V embryonic stem cell line using anti-human HNF-3b affinity-purified polyclonal antibody (Catalog # AF2400). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).
HNF-4a/NR2A1 HNF-4g/NR2A2 HNF-4a/NR2A1 Isoform 7-9 HOXA1 HSP60 HSP70 HSPH1 aa 595-733
Human Human Human Human Human/Mouse/Rat Human/Mouse Human/Mouse/Rat
H1415 K9218 N3224 H6939 Polyclonal 264233 242707 Polyclonal
Mouse IgG2A Mouse IgG2A Mouse IgG2A Mouse IgG1 Goat IgG Mouse IgG2B Mouse IgG2A Goat IgG
PP-H1415-00 PP-K9218-00 PP-N3224-00 PP-H6939-00 AF5014 MAB1800 MAB1663 AF4029
100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g
$380 $380 $380 $380 $355 $240 $240 $355
HSPH1 in Rat Cerebellum. HSP (Heat Shock Protein) H1 was detected in a cryostat section of rat cerebellum using anti-human/mouse/rat HSPHI affinitypurified polyclonal antibody (Catalog # AF4029). The tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
HTRA2/Omi HVEM/TNFRSF14 cIAP-1/HIAP-2 cIAP-2/HIAP-1 ICA1
Human Human/Mouse/Rat Human Human Human Human
229926 Polyclonal 94804 Polyclonal Polyclonal 315304 Polyclonal Polyclonal BBIG-I1 BBIG-I1 Polyclonal Polyclonal Polyclonal
Mouse IgG2B Rabbit IgG Mouse IgG1 Goat IgG Goat IgG Mouse IgG1 Goat IgG Goat Serum Mouse IgG1 Mouse IgG1 Goat IgG Goat IgG Goat IgG
MAB1458 AF1458 MAB3561 AF8181 AF8171 MAB817 AF4084 BBA17 BBA3 BBA4 AF796 BAF796 AF583
100 g 100 g 500 g 100 g 100 g 100 g 100 g 500 L 100 g 1 mg 100 g 50 g 100 g
$240 $285 $325 $355 $355 $240 $355 $195 $230 $840 $355 $380 $355
Human ICAM-1/CD54 Mouse Rat
58

MOLECULE ICAM-2/CD102 ICAM-3/CD50 ICAM-5 ICAM-5 ICOS ID2 IFITM1 IFITM3/Fragilis SPECIES Mouse Human Human Mouse Human Human Human Mouse LABEL None Biotin None None None Biotin None None None None CLONE Polyclonal Polyclonal ICAM-3.3 283607 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal HOST/TYPE Goat IgG Goat IgG Mouse IgG1 Mouse IgG2A Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG CATALOG # AF774 BAF774 BBA15 MAB1950 AF1173 BAF1173 AF169 AF4660 AF4827 AF3377 SIZE 100 g 50 g 200 g 100 g 100 g 50 g 100 g 100 g 100 g 100 g PRICE $355 $380 $315 $240 $355 $380 $355 $355 $355 $355
IFITM3/Fragilis in Embryonic Mouse Ovary. Interferon-induced transmembrane protein 3 (IFITM3) was detected in an E13.5 mouse embryonic ovary section using anti-mouse IFITM3 affinity-purified polyclonal antibody (Catalog # AF3377). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).
None None None None Fluorescein None Human None None IFN-a None None None None None Mouse Fluorescein None None None Biotin None None None
MMHA-3 MMHA-1 MMHA-8 MMHA-11 MMHA-11 MMHA-13 MMHA-17 MMHA-6 MMHA-9 MMHA-14 Polyclonal Polyclonal Polyclonal RMMA-1 Polyclonal K9 F17 Polyclonal Polyclonal MMHAR-2 Polyclonal
Mouse IgG1 Mouse IgG1 Mouse IgG2B Mouse IgG1 Mouse IgG1 Mouse IgG1 Mouse IgG1 Mouse IgG1 Mouse IgG1 Mouse IgG1 Sheep Serum Rabbit Serum Rabbit Serum Rat IgG1 Rabbit Serum Mouse IgG1 Mouse IgG1 Goat IgG Rabbit IgG Mouse IgG2A Rabbit Serum
21101-1 21105-1 21110-1 21112-1 21112-3 21116-1 21118-1 21125-1 21127-1 21129-1 31100-1 31101-1 31130-1 22100-3 32100-1 27100-1 27105-1 BAF3039 PPS074 21385-1 31385-1
50 g 50 g 50 g 50 g 50 Tests 50 g 50 g 50 g 50 g 50 g 100000 U 100000 U 20000 U 100 L 20000 U 500 g 500 g 50 g 100 L 50 g 50 g
$195 $195 $195 $250 $300 $195 $195 $195 $195 $195 $235 $235 $215 $355 $325 $315 $315 $380 $355 $330 $250
Porcine IFN-a/b R1 Phospho-IFN-a/b R1 (S535/S539) IFN-a/b R2 Mouse Human/Mouse/Rat/Bovine/ Canine/Primate/Sheep Human
59

MOLECULE SPECIES LABEL Fluorescein None None None Human IFN-b None None None None None Mouse Fluorescein None None None None Biotin None None Human Biotin None None Mouse Porcine Rat None Biotin None CLONE MMHB-3 MMHB-1 MMHB-12 MMHB-13 MMHB-14 MMHB-15 MMHB-16 Polyclonal Polyclonal RMMB-1 Polyclonal Polyclonal 345025 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 25718 25723 H22 Polyclonal Polyclonal HOST/TYPE Mouse IgG1 Mouse IgG2A Mouse IgG1 Mouse IgG3 Mouse IgG2A Mouse IgG2B Mouse IgG1 Sheep Serum Rabbit Serum Rat IgG1 Rabbit Serum Goat IgG Rat IgG2A Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG2A Mouse IgG2B Hamster IgG Goat IgG Goat IgG CATALOG # 21400-3 21405-1 21450-1 21455-1 21460-1 21465-1 21470-1 31400-1 31410-1 22400-3 32400-1 AF2300 MAB2300 AF781 BAF781 AF1586 AF-285-NA BAF285 MAB285 MAB2851 MAB4851 BAF985 AF-585-NA SIZE 50 Tests 50 g 50 g 50 g 50 g 50 g 50 g 20000 U 20000 U 100 L 20000 U 100 g 100 g 100 g 50 g 100 g 100 g 50 g 500 g 500 g 500 g 50 g 100 g PRICE $270 $195 $195 $195 $195 $195 $195 $280 $225 $320 $315 $355 $240 $355 $380 $355 $355 $380 $325 $325 $325 $380 $355
Bovine
Canine Equine IFN-g
IFN-g in Human Peripheral Blood Mononuclear Cells (PBMC). Interferon g (IFN-g) was detected in PMA-, ionomyocin-, and monensin-activated human PBMC using anti-human IFN-g affinity-purified polyclonal antibody (Catalog # AF-285-NA). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).
IFN-g R1/CD119
Human
None NL557
Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 133304 73009
Goat IgG Donkey IgG Donkey IgG Donkey IgG Donkey IgG Donkey IgG Donkey IgG Donkey IgG Donkey IgG Donkey IgG Goat IgG Goat IgG Goat IgG Donkey IgG Donkey IgG Donkey IgG Mouse IgG2A Mouse IgG2B
AF673 NL001 NL002 NL003 NL007 NL008 NL009 NL004 NL005 NL006 NL013 NL014 NL015 NL010 NL011 NL012 MAB0031 MAB0042
100 g 500 L 500 L 500 L 500 L 500 L 500 L 500 L 500 L 500 L 500 L 500 L 500 L 500 L 500 L 500 L 500 g 500 g
$355 $115 $115 $115 $115 $115 $115 $115 $115 $115 $115 $115 $115 $115 $115 $115 $170 $170
Goat
NL637 NL493 NL557
Mouse
NL637 NL493 NL557
IgG NorthernLights-conjugated
Rabbit
NL637 NL493 NL557
Rat
NL637 NL493 NL557
Sheep
NL637 NL493
IgG2A IgG2B
Mouse Mouse
None None
60

MOLECULE SPECIES LABEL NL557 IgY NorthernLights-conjugated Chicken NL637 NL493 IGFBP-1 IGFBP-3 Mouse Human None None None None Biotin None None None CLONE Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 84728 Polyclonal Polyclonal Polyclonal 164503 Polyclonal HOST/TYPE Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG2B Goat IgG Goat IgG Goat IgG Mouse IgG2B Goat IgG CATALOG # NL016 NL017 NL018 AF1240 AF675 MAB305 AF804 BAF804 AF875 MAB875 AF578 SIZE 500 L 500 L 500 L 100 g 100 g 500 g 100 g 50 g 100 g 500 g 100 g PRICE $115 $115 $115 $355 $355 $325 $355 $380 $355 $325 $355
IGFBP-4
Human
IGFBP-5
Human Mouse
IGFBP-5 in Mouse Ovary. Insulin-like Growth Factor Binding Protein 5 (IGFBP-5) was detected in a cryostat section of mouse ovary (follicle with oocyte) using anti-mouse IGFBP-5 affinity-purified polyclonal antibody (Catalog # AF578). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
IGFBP-6 IGFBP-L1
Mouse Mouse
None None Biotin None Biotin None None None None None None None Biotin None None None None Biotin None None None None None None
Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 292917 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 307707 Polyclonal 404619 404617 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 346909 Polyclonal Polyclonal 4414 Polyclonal
Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Rat IgG1 Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Rat IgG2A Goat IgG Rat IgG1 Rat IgG1 Goat IgG Goat IgG Goat IgG Sheep IgG Goat IgG Goat IgG Mouse IgG1 Goat IgG Goat IgG Mouse IgG2A Goat IgG
AF776 AF4130 BAF4130 AF1334 BAF1334 MAB21201 AF-291-NA AF791 AF-305-NA AF-292-NA AF792 AF2447 BAM3368 AF3678 MAB4164 MAB41641 AF3117 BAF3117 AF4984 AF3768 AF2684 AF3199 MAB3199 AF-200-NA BAF200 MAB200 AF-400-NA
100 g 100 g 50 g 100 g 50 g 100 g 250 g 100 g 100 g 250 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 50 g 100 g 100 g 100 g 100 g 100 g 100 g 50 g 500 g 100 g
$355 $355 $380 $355 $380 $240 $355 $355 $355 $355 $355 $355 $380 $355 $240 $240 $355 $380 $355 $355 $355 $355 $240 $355 $380 $325 $355
IGFBP-rp1/IGFBP-7
Human Mouse
IGF-I IGF-I R IGF-II IGF-II R IGSF2/CD101 IGSF4B IGSF4C
Human Mouse Human Human Mouse Human Mouse Human Human
IGSF8 Ikaros IKKa aa 375-745 IKKg IKKe
Mouse Human Human/Mouse/Rat Human/Mouse/Rat Human Human/Mouse/Rat
IL-1a/IL-1F1
Human
Biotin None
Mouse
None
61

MOLECULE SPECIES Feline LABEL None Biotin None None Human IL-1b/IL-1F2 Biotin None None Mouse None None Rat Biotin None
IL-1b in Human Peripheral Blood Mononuclear Cells (PBMCs). Interleukin-1b (IL-1b) was detected in LPS-stimulated human PBMCs using anti-human IL-1b affinity-purified polyclonal antibody (Catalog # AF-201-NA). The cells were stained using NorthernLights-557 anti-goat secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).
CLONE Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 8516 2805 Polyclonal Polyclonal Polyclonal 38139
HOST/TYPE Goat IgG Goat IgG Total Goat IgG Goat IgG Goat IgG Mouse IgG1 Mouse IgG1 Goat IgG Goat IgG Goat IgG Mouse IgG1
CATALOG # AF1796 BAF1796 AB-201-NA AF-201-NA BAF201 MAB201 MAB601 AF-401-NA AF-501-NA BAF501 MAB501
SIZE 100 g 50 g 1 mg 100 g 50 g 500 g 500 g 100 g 100 g 50 g 500 g
PRICE $355 $380 $315 $355 $380 $325 $325 $355 $355 $380 $325
IL-1b in Rat Splenocytes. Interleukin 1b (IL-1b) was detected in LPS-stimulated rat splenocytes using anti-rat IL-1b affinity-purified polyclonal antibody (Catalog # AF-501-NA). Cells were stained using NorthernLights-557 anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained (green).
IL-1b/IL-1F2 NorthernLights-conjugated
Human Equine
NL557 None None None None None Biotin None Biotin None None None None
Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 32437 Polyclonal
Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG2A Goat IgG
NL201R AF2466 AF-280-NA AF780 AF1275 AF1078 BAF1078 AF1099 BAF1099 AF771 AF-263-NA MAB263 AF563
500 L 100 g 100 g 100 g 100 g 100 g 50 g 100 g 50 g 100 g 100 g 500 g 100 g
$350 $355 $355 $355 $355 $355 $380 $355 $380 $355 $355 $325 $355
IL-1ra/IL-1F3
Human Porcine
IL-1F5/FIL1d IL-1F6/FIL1e
Human Human
IL-1F8/FIL1 IL-1 RI
Human Mouse Human Mouse
IL-1 RII
IL-1 RII in Human Peripheral Blood Mononuclear Cells (PBMCs). Interleukin-1 receptor II (IL-1 RII) was detected in human PBMCs by indirect immunofluorescent staining using anti-human IL-1 RII affinitypurified polyclonal antibody (Catalog # AF-263-NA) and counterstained (green).
IL-1 RAPL2/IL-1 R9
Human
None
Polyclonal
Goat IgG
AF1007
100 g
$355
62

MOLECULE SPECIES Bovine Canine Equine LABEL None Biotin None Biotin None Biotin None Human Biotin None None Porcine Rat None Biotin None CLONE Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 5334 5355 Polyclonal Polyclonal Polyclonal HOST/TYPE Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG1 Mouse IgG2A Goat IgG Goat IgG Goat IgG CATALOG # AF2465 BAF1815 AF1613 BAF1613 AF1890 BAF1890 AF-202-NA BAF202 MAB202 MAB602 AF652 BAF652 AF-502-NA SIZE 100 g 50 g 100 g 50 g 100 g 50 g 100 g 50 g 500 g 500 g 100 g 50 g 100 g PRICE $355 $380 $355 $380 $355 $380 $355 $380 $325 $325 $355 $380 $355
Feline IL-2
IL-2 in Human Peripheral Blood Mononuclear Cells (PBMCs). Interleukin-2 (IL-2) was detected in PMA- and ionomycin-stimulated human PBMCs using biotinylated anti-human IL-2 affinity-purified polyclonal antibody (Catalog # BAF202; red) and counterstained green.
Human IL-2 Ra Mouse Rat
None None None None
22722 24204 Polyclonal Polyclonal
Mouse IgG1 Mouse IgG1 Goat IgG Sheep IgG
MAB223 MAB623 AF2438 AF5156
500 g 500 g 100 g 100 g
$325 $325 $355 $355
IL-2 Ra in Mouse Spleen. Interleukin-2 Receptor a (IL-2 Ra) was detected in cryostat tissue sections of mouse spleen using anti-mouse IL-2 Ra affinity-purified polyclonal antibody (Catalog # AF2438). Tissues were stained using NorthernLights-557 donkey antigoat IgG secondary antibody (Catalog # NL001; red). Tissues were counterstained green.
IL-2 Rb
Human
None None
27302 Polyclonal Polyclonal 4806 Polyclonal Polyclonal 32703
Mouse IgG1 Total Goat IgG Goat IgG Mouse IgG1 Goat IgG Goat IgG Mouse IgG1
MAB224 AB-203-NA AF-203-NA MAB203 AF2524 BAF2524 MAB301
500 g 1 mg 100 g 500 g 100 g 50 g 500 g
$325 $355 $355 $325 $355 $380 $325
Human IL-3 Rat IL-3 Ra Human
63

MOLECULE SPECIES Bovine Equine IL-4 Human Porcine Rat LABEL None None Biotin None None Biotin CLONE Polyclonal Polyclonal Polyclonal 3007 Polyclonal Polyclonal HOST/TYPE Goat IgG Goat IgG Goat IgG Mouse IgG1 Goat IgG Goat IgG CATALOG # AF2469 AF1809 BAF204 MAB304 AF654 BAF504 SIZE 100 g 100 g 50 g 500 g 100 g 50 g PRICE $355 $355 $380 $325 $355 $380
IL-4 in Human Lymph Node. Interleukin-4 (IL-4) was detected in a cryostat tissue section of frozen human lymph node using anti-human IL-4 monoclonal antibody (Catalog # MAB304). Tissues were stained using anti-mouse secondary antibody (brown) and counterstained with hematoxylin (blue).
IL-4 Ra
Human Canine Equine Feline
None None None None None None None None None None
25463 Polyclonal Polyclonal Polyclonal 9906 Polyclonal Polyclonal Polyclonal 299298 Polyclonal Polyclonal 247002 Polyclonal Polyclonal 341031 Polyclonal Polyclonal 1936 Polyclonal 77830 Polyclonal
Mouse IgG2A Goat IgG Goat IgG Goat IgG Mouse IgG1 Goat IgG Goat IgG Goat IgG Mouse IgG1 Goat IgG Goat IgG Mouse IgG2B Goat IgG Goat IgG Mouse IgG2B Goat IgG Goat IgG Mouse IgG2B Goat IgG Mouse IgG1 Goat IgG
MAB230 AF1964 AF2470 AF1795 MAB605 AF-405-NA AF3137 AF545 MAB5452 AF1609 BAF1609 MAB16091 AF1886 AF2305 MAB23051 AF-206-NA BAF206 MAB2061 AF-406-NA MAB686 AF506
500 g 100 g 100 g 100 g 500 g 100 g 100 g 100 g 100 g 100 g 50 g 500 g 100 g 100 g 100 g 100 g 50 g 500 g 100 g 500 g 100 g
$325 $355 $355 $355 $325 $355 $355 $355 $240 $355 $380 $325 $355 $355 $240 $355 $380 $325 $355 $325 $355
IL-5
Human Mouse Porcine Rat
Canine
Biotin None
Equine Feline IL-6 Human
None None None None Biotin None
Mouse Porcine Rat
None None None
IL-6 in Porcine Peripheral Blood Mononuclear Cells. Interleukin-6 (IL-6) was detected in porcine PBMCs using anti-porcine IL-6 monoclonal antibody (Catalog # MAB686). Cells were stained using NorthernLights-557 donkey anti-mouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).
None IL-6 Ra Mouse Biotin None None
Polyclonal 255821 255821 255820
Goat IgG Rat IgG2A Rat IgG2A Rat IgG1
AF1830 BAM1830 MAB1830 MAB18301
100 g 100 g 500 g 100 g
$355 $380 $325 $240
64

MOLECULE IL-7 CXCR1/IL-8 RA CXCR2/IL-8 RB SPECIES Human Human Human Equine Human IL-10 Porcine Rat IL-10 Ra Human LABEL None None None None None None None None None None IL-12 Human Biotin None IL-12 p70 IL-12/IL-23 p40 IL-13 IL-13 Ra1 IL-13 Ra2 Human Feline Mouse Rat Human Human Mouse Human IL-15 Mouse Human Human Human Human Mouse IL-17C IL-17D Human Human None None None Biotin None None None None None Biotin None None None None Biotin None None None None CLONE 7417 42705 48311 Polyclonal Polyclonal Polyclonal 262715 Polyclonal Polyclonal Polyclonal Polyclonal 24910 24945 Polyclonal Polyclonal 296202 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 246018 HOST/TYPE Mouse IgG1 Mouse IgG2A Mouse IgG2A Goat IgG Goat IgG Goat IgG Mouse IgG2B Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG1 Mouse IgG1 Goat IgG Goat IgG Mouse IgG2B Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG2B CATALOG # MAB207 MAB330 MAB331 AF1605 AF-217-NA AF693 MAB6932 AF519 AF-274-NA AF-219-NA BAF219 MAB219 MAB611 AF1954 AF-413-NA BAM1945 AF152 AF146 AF539 AF315 AF447 BAF447 AF247 AF-316-PB AF-317-NA AF1207 BAF1207 AF1040 AF1234 AF1504 MAB15041 SIZE 500 g 500 g 500 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 500 g 500 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 50 g 100 g 100 g 100 g 100 g 50 g 100 g 100 g 100 g 100 g PRICE $325 $325 $325 $355 $355 $355 $240 $355 $355 $355 $380 $325 $325 $355 $355 $380 $355 $355 $355 $355 $355 $380 $355 $355 $355 $355 $380 $355 $355 $355 $240
IL-15 Ra IL-16 C-terminal Peptide IL-17
IL-17B R
IL-17D in Human Heart. Interleukin-17D (IL-17D) was detected in a paraffin-embedded human heart section using anti-human IL-17D monoclonal antibody (Catalog # MAB15041). The tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).
IL-17F
Human Mouse
None None
Goat IgG Goat IgG
AF1335 AF2057
100 g 100 g
$355 $355
65

MOLECULE SPECIES Human IL-17 RD/SEF Mouse LABEL None None None CLONE Polyclonal Polyclonal 400210 HOST/TYPE Goat IgG Goat IgG Rat IgG2A CATALOG # AF2275 AF2276 MAB2276 SIZE 100 g 100 g 100 g PRICE $355 $355 $240
IL-17 RD in Mouse Liver. Interleukin-17 Receptor D (IL-17 RD) was detected in frozen mouse liver sections using anti-mouse IL-17 RD monoclonal antibody (Catalog # MAB2276; red) and counterstained green.
IL-17 RD in Human Kidney. Interleukin-17 Receptor D (IL-17 RD) was detected in a paraffin-embedded human kidney sections using anti-human IL-17 RD affinity-purified polyclonal antibody (Catalog # AF2275). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Canine IL-18/IL-1F4 Porcine Rhesus Macaque IL-18 Ra/IL-1 R5 Human
Polyclonal 314811 Polyclonal Polyclonal Polyclonal 70625
Goat IgG Mouse IgG1 Goat IgG Goat IgG Goat IgG Mouse IgG1
AF2924 MAB29241 AF588 AF2548 AF840 MAB840
100 g 100 g 100 g 100 g 100 g 500 g
$355 $240 $355 $355 $355 $325
IL-18 R in Human Skin. Interleukin-18 Receptor (IL18 R) was detected in frozen sections of human skin using anti-human IL-18 R affinity-purified polyclonal antibody (Catalog # AF840). Tissue was stained using the anti-goat HRP-AEC Cell and Tissue Staining Kit (Catalog # CTS009; red) and counterstained with hematoxylin (blue).
IL-20 Ra IL-21
Human Mouse
None None
173707 Polyclonal
Mouse IgG1 Goat IgG
MAB11761 AF594
500 g 100 g
$325 $355
IL-21 in Mouse Splenocytes. Interleukin-21 (IL-21) was detected in mouse splenocytes stimulated with concanavalin A using anti-mouse IL-21 affinity-purified polyclonal antibody (Catalog # AF594). Cells were stained using NorthernLights-557 anti-goat secondary antibody (Catalog # NL001; red) and counterstained green.
IL-21 R Subunit IL-22 IL-22 Ra1
Mouse Human Human Mouse Human
None None None None None None Biotin None None None None None
Polyclonal Polyclonal 305405 496514 283123 Polyclonal 303308 Polyclonal Polyclonal Polyclonal 308202 Polyclonal
Goat IgG Goat IgG Mouse IgG1 Rat IgG2A Mouse IgG2B Goat IgG Rat IgG2A Goat IgG Goat IgG Goat IgG Mouse IgG1 Goat IgG
AF596 AF782 MAB2770 MAB42941 MAB19651 AF2786 BAM2786 AF1834 AF1587 AF2824 MAB28241 AF2769
100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g
$355 $355 $240 $240 $240 $355 $380 $355 $355 $355 $240 $355
IL-24
Mouse Mouse Human Human Human
IL-27 p28 IL-28A/IFN-2 IL-31 IL-31 RA
66

MOLECULE SPECIES Human Mouse IL-33 Propeptide Human Mouse LABEL None None None None None CLONE Polyclonal 390412 Polyclonal Polyclonal Polyclonal HOST/TYPE Goat IgG Rat IgG2B Goat IgG Goat IgG Sheep IgG CATALOG # AF3625 MAB3625 AF3626 AF4810 AF5010 SIZE 100 g 100 g 100 g 100 g 100 g PRICE $355 $240 $355 $355 $355
IL-33
IL-33 Propeptide in Human Umbilical Vein Endothelial Cells. Interleukin-33 (IL-33) Propeptide was detected in HUVECs using anti-human IL-33 Propeptide affinity-purified polyclonal antibody (Catalog # AF4810). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).
Indian Hedgehog N-Terminus ING1 Insulin Proinsulin
Mouse Human Human/Mouse/Bovine Human/Mouse
None None None Biotin None None None None None Biotin
Polyclonal Polyclonal 182410 253627 253627 Polyclonal 243524 Polyclonal Polyclonal HAS3 HAS3 430903 Polyclonal Polyclonal 386629 IA3 IA3 7.2R 7.2R
Goat IgG Goat IgG Rat IgG2A Mouse IgG2A Mouse IgG2A Goat IgG Mouse IgG2B Goat IgG Sheep IgG Mouse IgG2A Mouse IgG2A Rat IgG2A Sheep IgG Sheep IgG Rat IgG2A Mouse IgG1 Mouse IgG1 Mouse IgG1 Mouse IgG1
AF1705 AF5758 MAB1417 BAM13361 MAB13361 AF1544 MAB1544 AF3055 AF5676 BAM1233 MAB1233 MAB12332 AF1740 BAF1740 MAB41181 BAM1345 MAB1345 BAM1354 MAB1354
$355 $355 $240 $240 $240 $355 $240 $355 $355 $295 $325 $240 $355 $380 $240 $295 $325 $295 $325
Insulin R/CD220 INSRR Integrin a1/CD49a
Human Human Human
Human Integrin a2/CD49b Mouse Integrin a2b/CD41 Integrin a3/CD49c Mouse Human
None None None Biotin None Biotin None Biotin None
Integrin a4/CD49d
Human
Integrin a4/CD49d in Human U937 Cells. Integrin a4/CD49d was detected in human U937 lymphoma cell line using anti-human integrin a4/CD49d monoclonal antibody (Catalog # MAB1354). Cells were stained using NorthernLights-557 donkey antimouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).
Integrin a5/CD49e
Human Mouse
None None
238307 235112
Mouse IgG1 Rat IgG2B
MAB1864 MAB1754
100 g 100 g
$240 $240
Integrin a5 in Human Fibrosarcoma Cell Line (HT1080). Integrin a5 was detected in HT1080 human fibrosarcoma cells using anti-human integrin a5 monoclonal antibody (Catalog # MAB1219). Cells were stained using NorthernLights-557 donkey antimouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).
67

MOLECULE Integrin a6/CD49f SPECIES Human Human/Mouse/Bovine Mouse LABEL None None None Biotin Biotin Human Integrin aM/CD11b Mouse None None Biotin None None Integrin aV/CD51 Human Biotin None None Integrin aVb3 Integrin aVb5 Integrin b1/CD29 Human Human Human None None None None Human Integrin b1/CD29 Mouse None None None None CLONE MP4F10 GoH3 Polyclonal Polyclonal ICRF44 ICRF44 238446 M1/70 M1/70 Polyclonal Polyclonal P2W7 273210 23C6 P5H9 4B7R P5D2 P4G11 419127 Polyclonal 265917 HOST/TYPE Mouse IgG2B Rat IgG2A Goat IgG Goat IgG Mouse IgG1 Mouse IgG1 Mouse IgG2B Rat IgG2B Rat IgG2B Goat IgG Goat IgG Mouse IgG1 Mouse IgG1 Mouse IgG1 Mouse IgG1 Mouse IgG1 Mouse IgG1 Mouse IgG1 Mouse IgG2B Goat IgG Rat IgG2A CATALOG # MAB1350 MAB13501 AF4076 BAF4076 BAM1699 MAB1699 MAB16991 BAM1124 MAB1124 AF1219 BAF1219 MAB1219 MAB12191 MAB3050 MAB2528 MAB1778 MAB17781 MAB17782 MAB17783 AF2405 MAB2405 SIZE 500 g 100 g 100 g 50 g 100 g 500 g 100 g 250 g 500 g 100 g 50 g 500 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g PRICE $325 $240 $355 $380 $240 $325 $240 $380 $325 $355 $380 $325 $240 $240 $240 $240 $240 $240 $240 $355 $240
Integrin a8
Integrin b1, Orexin b, and p38a in Mouse Brainstem. Cryostat sections of mouse brainstem were labeled with anti-human Orexin B monoclonal antibody (Catalog # MAB734), anti-human/mouse/rat p38a affinitypurified polyclonal antibody (Catalog # AF8691), and anti-mouse Integrin b1 affinity-purified polyclonal antibody (Catalog #AF2405). Tissue was stained with NorthernLights (NL)-conjugated secondary polyclonal antibodies: NL-493 donkey anti-rabbit (Catalog # NL006; green), NL-557 donkey anti-mouse (Catalog # NL007; red), and NL-637 donkey anti-goat (Catalog # NL002; blue). Immunohistochemical image of integrin b1 expression is pseudo-colored for presentation.
Integrin b2/CD18
Human Human Mouse
None None None None None Biotin None
Polyclonal Polyclonal 422325 308601 Polyclonal Polyclonal Polyclonal Polyclonal 437211 Polyclonal Polyclonal 268622 473207
Goat IgG Sheep IgG Mouse IgG2B Rat IgG2A Sheep IgG Sheep IgG Sheep IgG Sheep IgG Mouse IgG2B Goat IgG Goat IgG Rat IgG2A Mouse IgG1
AF1730 AF4060 MAB4060 MAB4054 AF3824 BAF3824 AF4155 BAF4155 MAB4155 AF2389 BAF2389 MAB2389 MAB4669
100 g 100 g 100 g 100 g 100 g 50 g 100 g 50 g 100 g 100 g 50 g 100 g 100 g
$355 $355 $240 $240 $355 $380 $355 $380 $240 $355 $380 $240 $240
Integrin b4/CD104
Integrin b5
Human
Human Integrin b6 Mouse
Biotin None None Biotin None
Integrin b7
Human
None
68

MOLECULE Integrin b8 SPECIES Human LABEL None CLONE 416911 HOST/TYPE Mouse IgG1 CATALOG # MAB47751 SIZE 100 g PRICE $240
Integrin b8 in Human A172 Cells. Integrin b8 was detected in regions of membrane ruffling in A172 human glioblastoma cells using anti-human Integrin b8 monoclonal antibody (Catalog # MAB4775). Cells were stained using NorthernLights-557 donkey anti-mouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).
Intelectin-1/Omentin
Human
None
Polyclonal
Sheep IgG
AF4254
100 g
$355
Intelectin-1 in Mouse Intestine. Intelectin-1 was detected in a section of mouse intestine using antimouse Intelectin-1 affinity-purified polyclonal antibody (Catalog # AF4254; red). The tissue was stained using NorthernLights-557 anti-sheep secondary antibody (Catalog # NL010) and the nuclei counterstained with DAPI (blue).
IRAK4 IRF3 IRF5 aa 214-395 IRF8 IRS-1 ISG15/UCRP aa 2-157 Islet-1 Islet-1 NorthernLights-conjugated Islet-2
Human Human Human Human Human Human Human Human Human
None None None None None None None NL557 None Biotin
Polyclonal Polyclonal Polyclonal Polyclonal 395601 539442 Polyclonal Polyclonal Polyclonal Polyclonal
Goat IgG Goat IgG Sheep IgG Sheep IgG Mouse IgG1 Mouse IgG2B Goat IgG Goat IgG Sheep IgG Sheep IgG
AF3919 AF4019 AF4508 AF5117 MAB39781 MAB4845 AF1837 NL1837R AF4244 BAF4244
100 g 100 g 100 g 100 g 100 g 100 g 100 g 500 L 100 g 50 g
$355 $355 $355 $355 $240 $240 $355 $350 $355 $380
Islet-2 and Plexin A3 in Embryonic Mouse Spinal Cord. Islet-2 and Plexin A3 were detected in a section of embryonic mouse spinal cord using biotinylated anti-human Islet-2 affinity-purified polyclonal antibody (Catalog # BAF4244) and anti-mouse Plexin A3 (Catalog # AF4075). The tissue was stained using NorthernLights-493 streptavidin (Catalog # NL997; green) and NorthernLights-557 donkey anti-goat IgG affinity-purified polyclonal antibody (Catalog # NL001; red), respectively. The nuclei were stained using DAPI (blue).
ISLR-2 Jagged 1 Jagged 2 Jak1 aa 14-55 Jak1 aa 20-274
Mouse Human Human Mouse/Rat Human/Mouse/Rat
Sheep IgG Goat IgG Goat IgG Goat IgG Rat IgG2B
AF4650 AF1277 AF1726 AF602 MAB4260
100 g 100 g 100 g 100 g 100 g
$355 $355 $355 $355 $240
Jak1 in Human Skin. Jak1 was detected in a section of paraffin-embedded human epidermis using antihuman Jak1 monoclonal antibody (Catalog # MAB4260). Tissue was stained using anti-rat HRP-DAB reagents (brown) and counterstained with hematoxylin (blue).
69

MOLECULE JAM-A JAM-C JNK Pan Specific Phospho-JNK (T183/Y185) JNK1/JNK2 JNK1 JNK2 c-Jun aa 1-331 Kallikrein 3/PSA Kallikrein 5 Kallikrein 6/Neurosin SPECIES Human Mouse Mouse Human/Mouse/Rat Human/Mouse/Rat Human/Mouse/Rat Human/Mouse/Rat Human/Mouse/Rat Human/Mouse Human Human Human LABEL None None None None None None None None None None None None CLONE Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 252323 228601 252320 320831 Polyclonal Polyclonal Polyclonal HOST/TYPE Goat IgG Goat IgG Goat IgG Rabbit IgG Rabbit IgG Mouse IgG2A Mouse IgG1 Mouse IgG2B Mouse IgG1 Goat IgG Goat IgG Goat IgG CATALOG # AF1103 AF1077 AF1213 AF1387 AF1205 MAB2076 MAB17761 MAB1846 MAB2670 AF1344 AF1108 AF2008 SIZE 100 g 100 g 100 g 100 g 50 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g PRICE $355 $355 $355 $285 $285 $240 $240 $240 $240 $355 $355 $355
Kallikrein 6 in Human Breast Cancer. Kallikrein 6/ Neurosin was detected in paraffin-embedded human breast cancer tissue sections using anti-human Kallikrein 6 affinity-purified polyclonal antibody (Catalog # AF2008). Tissues were stained using anti-goat HRPDAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Kallikrein 7
Human
None
Polyclonal
Goat IgG
AF2624
100 g
$355
Kallikrein 7 in Normal Human Skin. Kallikrein 7 (KLK7) was detected in paraffin-embedded normal human skin sections using anti-human KLK7 affinitypurified polyclonal antibody (Catalog # AF2624). Tissue was stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Kallikrein 9
Human
None
Polyclonal
Goat IgG
AF3486
100 g
$355
Kallikrein 9 in Human Ovarian Cancer. Kallikrein 9 (KLK9) was detected in paraffin-embedded human ovarian cancer sections using anti-human KLK9 affinity-purified polyclonal antibody (Catalog # AF3486). Tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Kallikrein 11 Kallikrein 12 Kallikrein 13
Human Human Human
None None None None
Polyclonal 189308 Polyclonal Polyclonal
Goat IgG Mouse IgG3 Sheep IgG Goat IgG
AF1595 MAB1595 AF3095 AF2625
100 g 500 g 100 g 100 g
$355 $325 $355 $355
Kallikrein 13 in Human Prostate Cancer. Kallikrein 13 (KLK13) was detected in paraffin-embedded human prostate cancer sections using anti-human KLK13 affinity-purified polyclonal antibody (Catalog # AF2625). Tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
70

MOLECULE Kallikrein 15 KLF4 KLF5 Klotho SPECIES Human Human Mouse Human Mouse LABEL None None None None None None CLONE Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 236214 HOST/TYPE Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Rat IgG2A CATALOG # AF2540 AF3640 AF3158 AF3758 AF1819 MAB1819 SIZE 100 g 100 g 100 g 100 g 100 g 500 g PRICE $355 $355 $355 $355 $355 $325
Klotho in Mouse Kidney. Klotho was detected in a frozen section of mouse kidney tissue using antimouse Klotho affinity-purified polyclonal antibody (Catalog # AF1819). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
KOR
Human Human Mouse Mouse Mouse
None None None None Biotin None
387301 387310 Polyclonal Polyclonal Polyclonal AL-4
Mouse IgG1 Mouse IgG1 Goat IgG Goat IgG Goat IgG Rat IgG2A
MAB3895 MAB38951 AF2127 AF1647 BAF1647 MAB4656
100 g 100 g 100 g 100 g 50 g 100 g
$240 $240 $355 $355 $380 $240
Kremen-1 Kremen-1 Laminin a1
Laminin a1 in Mouse Decidua. Laminin a1 was detected in frozen sections of mouse E10.5 decidua using anti-mouse laminin a1 monoclonal antibody (Catalog # MAB4656; red). Tissue was counterstained with DAPI (blue).
Laminin a1 N-Terminus Domain VI Laminin a4 Laminin g1 Laminin S Laminin-1 Laminin-5
Human Mouse Human/Rat Human/Rat/Chicken Mouse Human
None None Biotin None None None None None
Polyclonal Polyclonal Polyclonal D18 C4 AL-2 AL-3 P3H9-2
Goat IgG Goat IgG Goat IgG Mouse IgG2A Mouse IgG1 Rat IgG1 Rat IgG1 Mouse IgG1
AF4187 AF3837 BAF3837 MAB2139 MAB2066 MAB2549 MAB25491 MAB2144
100 g 100 g 50 g 100 g 100 g 100 g 100 g 250 g
$355 $355 $380 $240 $295 $240 $240 $240
Laminin-5 in Human MCF10A Cells. Laminin-5 was detected in human MCF10A breast epithelial cell line using anti-human Laminin-5 monoclonal antibody (Catalog # MAB2144). Cells were stained using NorthernLights-557 donkey anti-mouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).
LAMP1/CD107a Lumenal Domain Langerin/CD207 Lck
Human Human Human Human
None None None None None Biotin
Polyclonal Polyclonal 355713 Polyclonal Polyclonal Polyclonal
Sheep IgG Goat IgG Mouse IgG2B Goat IgG Goat IgG Goat IgG
AF4800 AF2088 MAB3704 AF2148 AF2255 BAF2255
100 g 100 g 100 g 100 g 100 g 50 g
$355 $355 $240 $355 $355 $380
LDL R
Mouse
71

MOLECULE LEDGF Leptin/OB SPECIES Human Human Mouse Human Mouse LABEL None None None None None None CLONE Polyclonal Polyclonal Polyclonal Polyclonal 52263 Polyclonal HOST/TYPE Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG2B Goat IgG CATALOG # AF3468 AF398 AF498 AF389 MAB867 AF497 SIZE 100 g 100 g 100 g 100 g 500 g 100 g PRICE $355 $355 $355 $355 $325 $355
Leptin R
Leptin Receptor in Rat Brain. Leptin Receptor was detected in cryostat tissue sections of rat brain using anti-mouse Leptin Receptor affinity-purified polyclonal antibody (Catalog # AF497). Tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
LIF
Human Mouse
None None None None None None Biotin None None None None None None None Biotin None None None None None Biotin None
Polyclonal 9824 Polyclonal Polyclonal 320416 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 332237 Polyclonal 382007 Polyclonal Polyclonal Polyclonal Polyclonal 220310 234903 Polyclonal Polyclonal Polyclonal
Goat IgG Mouse IgG2B Total Goat IgG Goat IgG Mouse IgG2A Goat IgG Goat IgG Goat IgG Sheep IgG Goat IgG Mouse IgG1 Goat IgG Mouse IgG2A Goat IgG Goat IgG Goat IgG Goat IgG Rat IgG2A Rat IgG2B Goat IgG Goat IgG Sheep IgG
AF-250-NA MAB250 AB-449-NA AF4074 MAB2725 AF597 BAF597 AF3757 AF5104 AF3086 MAB30861 AF3679 MAB36791 AF3885 BAF3885 AF4064 AF1757 MAB1757 MAB2774 AF2726 BAF2726 AF4995
100 g 500 g 1 mg 100 g 100 g 100 g 50 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 50 g 100 g 100 g 500 g 100 g 100 g 50 g 100 g
$355 $325 $355 $355 $240 $355 $380 $355 $355 $355 $240 $355 $240 $355 $380 $355 $355 $325 $240 $355 $380 $355
LILRC1 LIM1 Limitin LIN-28 LIN-41 LINGO-1 LINGO-1 aa 40-556 LINGO-2
Rat Human Mouse Human Human Human Human Human
Lipin 1 Lipin 3 Lipocalin-2/NGAL LMIR3/CD300LF LMO2 LRRC4
Human Mouse Human Human Human Human
LRRC4 in Human Cerebellum. Leucine rich repeat/ LRR-containing protein 4 (LRRC4) was detected in paraffin-embedded sections of human cerebellum using anti-human LRRC4 affinity-purified polyclonal antibody (Catalog # AF4995). Tissue was stained using anti-sheep HRP-DAB secondary reagents (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).
72

MOLECULE LRRN1/NLRR-1 SPECIES Human LABEL None CLONE Polyclonal HOST/TYPE Sheep IgG CATALOG # AF4990 SIZE 100 g PRICE $355
LRRN1 in Mouse Somites. Leucine rich repeat neuronal 1 (LRRN1) was detected in a frozen section from E9.5 mouse somite using anti-human LRRN1 affinitypurified polyclonal antibody (Catalog # AF4990). Cells were stained using NorthernLights-557 donkey anti-sheep IgG secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue).
LRRTM2 Lumican
Human/Mouse Human Mouse Human
None None None None None None Biotin None None None None None Biotin None
Polyclonal Polyclonal Polyclonal PPZ0412 PPZ0412 Polyclonal Polyclonal Polyclonal 338716 Polyclonal 340113 Polyclonal Polyclonal Polyclonal Polyclonal 223322 Polyclonal Polyclonal Polyclonal Polyclonal
Sheep IgG Goat IgG Goat IgG Mouse IgG2A Mouse IgG2A Goat IgG Goat IgG Goat IgG Mouse IgG2B Goat IgG Mouse IgG2B Goat IgG Goat IgG Goat IgG Goat IgG Rat IgG2A Goat IgG Goat IgG Goat IgG Goat IgG
AF5589 AF2846 AF2745 PP-PPZ0412-00 PP-PPZ0412-10 AF1008 BAF1008 AF3206 MAB3206 AF3428 MAB3428 AF2089 BAF2089 AF2125 BAF2125 MAB2125 AF993 AF3917 AF538 BAF538
$355 $355 $355 $380 $380 $355 $380 $355 $240 $355 $240 $355 $380 $355 $380 $325 $355 $355 $355 $380
LXRa/NR1H3 Ligand Binding Domain
Lymphotoxin bR/TNFRSF3
Mouse Human/Mouse/Rat Human/Rat Human
Lyn aa 1-67
Lyp
Human LYVE-1 Mouse
Biotin None
MAdCAM-1 MafF MAG/Siglec-4a
Mouse Human Rat
MAG/Siglec-4a in Rat Cerebellum. Myelin-associated Glycoprotein (MAG/Siglec-4a) was detected in cryostat sections of rat cerebellum using anti-rat MAG affinity-purified polyclonal antibody (Catalog # AF538). Tissues were stained using the anti-goat HRP DAB Cell and Tissue Staining Kit (Catalog # CTS008); brown) and counterstained with hematoxylin (blue).
Mash1 Matrilin-2 MCAM/CD146 Mcl-1 aa 1-230 M-CSF R Phospho-M-CSF R (Y723) MD-1 MD-2 MDM2 MEK1/MEK2 Phospho-MEK1/MEK2 (MEK1 S218/S2220, MEK2 S222/S226)
Mouse Human Human Human Mouse Human Human Human Human/Mouse/Rat Human/Mouse/Rat Human/Mouse/Rat
None None None None Biotin None None None None Biotin None None None
Polyclonal 290622 Polyclonal Polyclonal Polyclonal 542808 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 239416 Polyclonal
Goat IgG Rat IgG2A Goat IgG Goat IgG Goat IgG Mouse IgG2B Sheep IgG Rabbit IgG Goat IgG Sheep IgG Rabbit IgG Mouse IgG2B Rabbit IgG
AF2567 MAB2567 AF3044 AF932 BAF932 MAB828 AF3818 AF3268 AF925 BAF1787 AF1244 MAB2678 AF2506
100 g 100 g 100 g 100 g 50 g 100 g 100 g 50 g 100 g 50 g 100 g 100 g 100 g
$355 $240 $355 $355 $380 $240 $355 $285 $355 $380 $285 $240 $285
73

MOLECULE MEK2 MELK Melusin Meprin a Subunit/MEP1A Meprin b Subunit/MEP1B SPECIES Human/Mouse/Rat Human Human Human Human Mouse LABEL None None None None None None None CLONE 300317 Polyclonal Polyclonal 308705 Polyclonal Polyclonal Polyclonal HOST/TYPE Mouse IgG2A Sheep IgG Goat IgG Mouse IgG2B Goat IgG Goat IgG Goat IgG CATALOG # MAB2855 AF4820 AF2740 MAB2740 AF3220 AF2895 AF3300 SIZE 100 g 100 g 100 g 100 g 100 g 100 g 100 g PRICE $240 $355 $355 $240 $355 $355 $355
Meprin b Subunit in Mouse Intestine. Meprin b Subunit (MEP1B) was detected in a frozen section of mouse intestine using anti-mouse MEP1B affinitypurified polyclonal antibody (Catalog # AF3300). The tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
MESDC2
Human Mouse
None None
Sheep IgG Goat IgG
AF5577 AF4545
100 g 100 g
$355 $355
MESDC2 in Adult Mouse Testis. Mesoderm development candidate 2 (MESDC2) was detected in section of mouse testis using anti-mouse MESDC2 affinity-purified polyclonal antibody Catalog # AF4545). The tissue was stained using anti-goat NorthernLight-557 secondary antibody (Catalog # NL001, red) and the nuclei were counterstained with DAPI (blue).
Mesothelin Isoform 1, aa 296-580 Meteorin
Human Mouse
None None None
420404 Polyclonal 347505
Rat IgG2A Goat IgG Rat IgG2B
MAB32651 AF3475 MAB3475
100 g 100 g 100 g
$240 $355 $240
Meteorin in Rat Cortical Stem Cells. Meteorin was detected in 7-day differentiated rat cortical stem cells using anti-mouse Meteorin monoclonal antibody (Catalog # MAB3475). Cells were stained using NorthernLights-557 goat anti-rat IgG secondary antibody (Catalog # NL013; red) and counterstained with DAPI (blue).
MFRP mGluR1a C-Terminus mGluR3 mGluR5
Human Mouse Rat Human Human
291204 Polyclonal Polyclonal Polyclonal Polyclonal 464823
Rat IgG1 Goat IgG Rabbit IgG Sheep IgG Sheep IgG Mouse IgG2B
MAB19151 AF3445 PPS077 AF4668 AF4514 MAB45141
100 g 100 g 100 L 100 g 100 g 100 g
$240 $355 $355 $355 $355 $240
mGluR5 in Human Brain (Putamen). Metabotropic glutamate receptor 5 (mGluR5) was detected in a paraffin-embedded human brain (putamen) section using anti-human mGluR5 affinity-purified polyclonal antibody (Catalog # AF4514). The tissue was stained using anti-sheep HRP-DAB detection reagents (brown) and counterstained with hematoxylin (blue).
74

MOLECULE mGluR5/1a mGluR8 MIA SPECIES Human/Mouse/Rat Human Human LABEL None None None None None Biotin None None None None None None None None None None None None None None None None None None CLONE Polyclonal 476410 Polyclonal 294203 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal H3122 385707 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 319919 275909 323042 Polyclonal 304328 Polyclonal 324302 Polyclonal HOST/TYPE Rabbit IgG Mouse IgG2B Goat IgG Mouse IgG2B Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG2A Mouse IgG2B Goat IgG Goat IgG Sheep IgG Goat IgG Goat IgG Mouse IgG2A Mouse IgG2B Mouse IgG2B Rabbit IgG Rat IgG2B Goat IgG Mouse IgG2B Rabbit IgG CATALOG # PPS079 MAB5277 AF2050 MAB20501 AF1300 BAF1300 AF2946 AF2950 AF-258-PB PP-H3122-00 MAB4369 AF1446 AF2748 AF4749 AF1618 AF5769 MAB2610 MAB2515 MAB3390 AF2990 MAB1604 AF3579 MAB3579 AF3435 SIZE 100 L 100 g 100 g 100 g 100 g 50 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g PRICE $355 $240 $355 $240 $355 $380 $355 $355 $355 $380 $240 $355 $355 $355 $355 $355 $240 $240 $240 $285 $240 $355 $240 $285
MICA
Human Human Mouse Human Human Human Rat Human Human Rat Human Human Human/Mouse/Rat Human Human/Mouse/Rat Human/Mouse/Rat Human Human/Mouse/Rat
MICL/CLEC12A Midkine Mineralocorticoid R/NR3C2 Mineralocorticoid R/NR3C2 aa 1-670 MIS/AMH MIS/AMH Propeptide MIS RII MITF MIXL1 MKK3 MKK4 Phospho-MKK4 (S257/T261) MKK6 MKK7 MLK4a aa 560-570
Pan MLK-4 in Human Colon Cancer. Mixed lineage Kinase-4 (MLK-4) was detected in paraffin-embedded human colon cancer sections using anti-human/ mouse/rat pan MLK-4 affinity-purified polyclonal antibody (Catalog # AF3435). Tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013). Tissue was stained using antirabbit HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS005; brown) and counterstained with hematoxylin (blue).
MLX MMP-1 Pro MMP-1
Human Human Human Human Mouse/Rat
Polyclonal Polyclonal 36665 36660 Polyclonal 36006 Polyclonal Polyclonal 50647 10D6
Goat IgG Goat IgG Mouse IgG1 Mouse IgG1 Goat IgG Mouse IgG1 Goat IgG Goat IgG Mouse IgG1 Mouse IgG1
AF4186 AF901 MAB901 MAB900 AF902 MAB902 AF1488 AF513 MAB513 MAB905
100 g 100 g 200 g 200 g 100 g 200 g 100 g 100 g 500 g 200 g
$355 $355 $295 $295 $355 $295 $355 $355 $325 $295
MMP-2
MMP-3
Human
None None
MMP-3 in Human Osteosarcoma Cell Line (MG63). Matrix Metalloproteinase-3 (MMP-3) was detected in MG-63 cells using anti-human MMP-3 monoclonal antibody (Catalog # MAB513). Cells were stained using NorthernLights-557 donkey anti-mouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).
75

MOLECULE SPECIES Human Mouse Pro MMP-7 Human LABEL None None None None None MMP-8 Human Biotin None None Human MMP-9 None None None Mouse None Biotin None MMP-10 Human None None None MMP-12 Catalytic Domain MMP-12 Hemopexin Domain MMP-13 Human Human Human None None None None CLONE Polyclonal 111433 Polyclonal 6A4 Polyclonal Polyclonal 100608 Polyclonal Polyclonal 4H3 36020 Polyclonal Polyclonal Polyclonal 110304 117239 110316 82902 4D2 Polyclonal 87512 HOST/TYPE Goat IgG Mouse IgG2B Goat IgG Mouse IgG1 Goat IgG Goat IgG Mouse IgG2A Total Goat IgG Goat IgG Mouse IgG1 Mouse IgG1 Goat IgG Goat IgG Goat IgG Mouse IgG1 Mouse IgG2B Mouse IgG1 Mouse IgG2B Mouse IgG1 Goat IgG Mouse IgG1 CATALOG # AF907 MAB9071 AF2967 MAB907 AF908 BAF908 MAB9081 AB911 AF911 MAB911 MAB936 AF909 BAF909 AF910 MAB910 MAB9101 MAB9102 MAB919 MAB917 AF511 MAB511 SIZE 100 g 500 g 100 g 200 g 100 g 50 g 500 g 1 mg 100 g 200 g 500 g 100 g 50 g 100 g 500 g 500 g 500 g 500 g 200 g 100 g 500 g PRICE $355 $325 $355 $295 $355 $380 $325 $355 $355 $295 $325 $355 $380 $355 $325 $325 $325 $325 $295 $355 $325
MMP-7
MMP-13 in Human Ovarian Cancer Tissue. Matrix metalloproteinase-13 (MMP-13) producing cells in human ovarian cancer tissue were detected using anti-human MMP-13 affinity-purified antibody (Catalog # AF511). Tissues were stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and Hematoxylin (blue) counterstain.
Pro MMP-13 MMP-14 MMP-14 aa 165-175 MMP-15 MMP-15 Catalytic Domain MMP-24/MT5-MMP MMP-25/MT6-MMP Hemopexin Domain MMR
Human Human Human Human Human Human/Mouse Human Human Human
None None None None None None None None None None None Biotin None Biotin None None None None None None
87518 Polyclonal 128527 5H2 130522 130516 Polyclonal 141825 Polyclonal 309210 Polyclonal 296829 Polyclonal Polyclonal QCRL 252608 Polyclonal Polyclonal Polyclonal Polyclonal
Mouse IgG2B Goat IgG Mouse IgG2B Mouse IgG1 Mouse IgG1 Mouse IgG1 Total Rabbit IgG Mouse IgG1 Goat IgG Rat IgG2A Goat IgG Rat IgG2B Goat IgG Goat IgG Mouse IgG1 Mouse IgG2B Goat IgG Rabbit IgG Rabbit IgG Goat IgG
MAB913 AF918 MAB9181 MAB918 MAB9161 MAB916 AB924 MAB11421 AF2534 MAB2534 AF2395 BAM2439 AF2439 BAF2439 MAB19291 MAB2518 AF2518 AF1036 AF189 AF3826
$325 $355 $325 $325 $325 $325 $355 $325 $355 $240 $355 $380 $355 $380 $240 $240 $355 $285 $285 $355
MOG
Human/Mouse Mouse
MRP1 MSK1 Phospho-MSK1 (S212) Phospho-MSK2 (S196) MUCDHL
Human Human Human/Mouse Human Human/Mouse Human
76

MOLECULE Musashi-1 SPECIES Human LABEL None None CLONE Polyclonal 282613 HOST/TYPE Goat IgG Mouse IgG2B CATALOG # AF2628 MAB2628 SIZE 100 g 100 g PRICE $355 $240
Musashi-1 in Human Intestine. Musashi-1 was detected in a paraffin-embedded human intestine section using anti-human Musashi-1 affinity-purified polyclonal antibody (Catalog # AF2628). Tissues were stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Mxi1 c-Myc aa 66-201
Human Human Human
None None None None None None None None None
Polyclonal Polyclonal Polyclonal 316603 316902 Polyclonal Polyclonal Polyclonal 392105
Goat IgG Goat IgG Goat IgG Rat IgG2A Rat IgG2A Goat IgG Goat IgG Goat IgG Mouse IgG2B
AF4185 AF3696 AF2928 MAB2928 MAB3109 AF3109 AF3174 AF3667 MAB3174
100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g
$355 $355 $355 $240 $240 $355 $355 $355 $240
MyD88
Mouse Mouse/Rat
Myeloperoxidase/MPO Myeloperoxidase/MPO aa 279-745
Human Human/Mouse Human/Mouse
Myeloperoxidase in Human Spleen. Myeloperoxidase (MPO) was detected in paraffin-embedded human spleen tissue sections using anti-human/mouse MPO monoclonal antibody (Catalog # MAB3174) and Antigen Retrieval Reagent - Basic (Catalog # CTS013). The tissue was stained using anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).
Myosin Heavy Chain NAALADase-like 2/NAALADL2
MF20 Polyclonal Polyclonal Polyclonal Polyclonal
Mouse IgG2B Goat IgG Goat IgG Goat IgG Goat IgG
MAB4470 AF4665 AF1997 BAF1997 AF2729
100 g 100 g 50 g 50 g 100 g
$240 $355 $355 $395 $355
Nanog
Nanog in Human BG01V Embryonic Stem Cells. Nanog was detected in human BG01V embryonic stem cells using anti-human Nanog affinity-purified polyclonal antibody (Catalog # AF1997). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).
Nanog NorthernLights-conjugated NCAM-1/CD56 NCAM-L1 Nectin-3 Nectin-4
NL493 None None None None None
Polyclonal 301021 Polyclonal Polyclonal Polyclonal 337506
Goat IgG Mouse IgG2B Goat IgG Goat IgG Goat IgG Mouse IgG2A
NL1997G MAB24081 AF277 AF3064 AF2659 MAB26591
500 L 100 g 100 g 100 g 100 g 100 g
$350 $240 $355 $355 $355 $240
77

MOLECULE NEDD8 SPECIES Human/Mouse/Rat LABEL None CLONE Polyclonal HOST/TYPE Sheep IgG CATALOG # AF4936 SIZE 100 g PRICE $355
NEDD8 in Human Skeletal Muscle. Neural precursor cell-expressed developmentally down-regulated gene 8 (NEDD8) was detected in paraffin-embedded human skeletal muscle sections using anti-human/ mouse/rat NEDD8 affinity-purified polyclonal antibody (Catalog # AF4936). Tissue was stained using anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).
Neogenin Nephrin
Mouse Human Mouse
None None None
Goat IgG Sheep IgG Goat IgG
AF1079 AF4269 AF3159
100 g 100 g 100 g
$355 $355 $355
Nephrin in Mouse Kidney. Nephrin was detected in frozen sections of mouse kidney using anti-mouse nephrin affinity-purified polyclonal antibody (Catalog # AF3159). Tissue was stained using anti-goat IgG NorthernLights-557-conjugated secondary antibody (Catalog # NL001; red) and counterstained green.
Human Neprilysin/CD10 Human/Mouse Mouse Human Nestin Rat
None Biotin None None None None None
Polyclonal Polyclonal 200103 Polyclonal 196908 Polyclonal 307501
Goat IgG Goat IgG Rat IgG2B Goat IgG Mouse IgG1 Goat IgG Mouse IgG2A
AF1182 BAF1182 MAB1126 AF1126 MAB1259 AF2736 MAB2736
100 g 50 g 500 g 100 g 100 g 100 g 100 g
$355 $380 $325 $355 $240 $355 $240
Nestin in Undifferentiated Rat Neural Stem Cells. Nestin was detected in rat cortical stem cells using anti-rat Nestin monoclonal antibody (Catalog # MAB2736). The cells were stained using anti-mouse NorthernLights-557 secondary antibody (Catalog # NL007; red) and the nuclei counterstained with DAPI (blue).
Nestin NorthernLights-conjugated
Human Rat Human
NL637 NL557 None Biotin None Biotin
196908 307501 Polyclonal Polyclonal Polyclonal Polyclonal
Mouse IgG1 Mouse IgG2A Goat IgG Goat IgG Goat IgG Goat IgG
NL1259V NL2736R AF1254 BAF1254 AF1132 BAF1132
500 L 500 L 100 g 50 g 100 g 50 g
$345 $345 $355 $380 $355 $380
Netrin-4 Mouse
Netrin-4 in Mouse Brain. Netrin-4 was detected in frozen sections of mouse brain (cerebellum) using anti-mouse Netrin-4 affinity-purified polyclonal antibody (Catalog # AF1132). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Netrin-G2a
Mouse
None
349724
Rat IgG2A
MAB27441
100 g
$240
78

MOLECULE NRG1-a/HRG1-a EGF Domain NRG1-b1/HRG1-b1 EGF Domain Neuregulin-3/NRG3 EGF-like Domain Neurexin 3/NRXN3 Neuritin Neurocan NeuroD1 NF-H SPECIES Human Human Mouse Human/Mouse Human Mouse/Rat Human Human LABEL None None None None None None None Biotin None CLONE Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal HOST/TYPE Goat IgG Goat IgG Goat IgG Sheep IgG Goat IgG Sheep IgG Goat IgG Goat IgG Goat IgG CATALOG # AF-296-NA AF-396-NA AF753 AF5269 AF283 AF5800 AF2746 BAF2746 AF3108 SIZE 100 g 100 g 100 g 100 g 100 g 100 g 100 g 50 g 100 g PRICE $355 $355 $355 $355 $355 $355 $355 $380 $355
Neurofilament-H in Human Brain. Neurofilament-H (NF-H) was detected in paraffin-embedded human brain (cerebellum) tissue sections using anti-human NF-H affinity-purified polyclonal antibody (Catalog # AF3108). Tissues were stained using anti-goat HRPDAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
NF-L NF-M Neurogenin-2 Neuroglycan C/CSPG5
Human Human Human/Rat Human Mouse/Rat
268014 Polyclonal 7G4 Polyclonal Polyclonal
Mouse IgG2B Sheep IgG Mouse IgG2A Sheep IgG Goat IgG
MAB2216 AF3029 MAB3314 AF5685 AF5665
100 g 100 g 100 g 100 g 100 g
$240 $355 $240 $355 $355
Neuroglycan C in Mouse Cerebellum. Neuroglycan C was detected in a section of adult mouse cerebellum using anti-mouse/rat Neuroglycan affinity-purified polyclonal antibody (Catalog # AF5665). The cells were stained using NorthernLights-557 anti-goat lgG secondary antibody (Catalog # NL001; red) and the nuclei counterstained with DAPI (blue).
Neuronal Pentraxin R Neurophysin II
Human Human Human
Sheep IgG Goat IgG Sheep IgG Goat IgG Mouse IgG1
AF4414 AF009 AF3870 AF566 MAB5661
100 g 100 g 100 g 100 g 500 g
$355 $355 $355 $355 $325
Neuropilin-1/BDCA4
Rat
Neuropilin-1 Expression in Mouse Embryonic Spinal Cord. Neuropilin-1 was detected in a cryostat section of mouse embryonic spinal cord (E15) using anti-rat Neuropilin-1 affinity-purified polyclonal antibody (Catalog # AF566). Tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Neuropilin-2
Human
None
Polyclonal
Goat IgG
AF2215
100 g
$355
79

MOLECULE Neuroplastin Isoform 1 SPECIES Human LABEL None CLONE Polyclonal HOST/TYPE Sheep IgG CATALOG # AF5174 SIZE 100 g PRICE $355
Neuroplastin in Human Cerebellum. Neuroplastin was detected in a paraffin-embedded human cerebellum section using anti-human Neuroplastin affinity-purified polyclonal antibody (Catalog # AF5174). The tissue was stained using anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).
Neuroplastin 65 Neurotrimin
Human/Mouse Human Human Human/Mouse Human Human Human Human Rat Human
None None Biotin None None None None None None None None None Biotin None None
Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 291319 LHM-2 Polyclonal Polyclonal 74902 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 302117 Polyclonal Polyclonal Polyclonal 191004 Polyclonal 195314 Polyclonal Polyclonal 259416 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 106814 Polyclonal Polyclonal
Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG1 Mouse IgG1 Goat IgG Goat IgG Mouse IgG1 Goat IgG Goat IgG Sheep IgG Goat IgG Goat IgG Mouse IgG1 Goat IgG Goat IgG Sheep IgG Rat IgG2B Goat IgG Mouse IgG2B Goat IgG Goat IgG Mouse IgG1 Goat IgG Rabbit IgG Rabbit IgG Rabbit IgG Rabbit IgG Rabbit IgG Rabbit IgG Rabbit IgG Mouse IgG2B Goat IgG Goat IgG
AF5360 AF1235 BAF1235 AF387 AF477 AF5616 MAB28881 MAB2585 AF-256-NA AF-556-NA MAB367 AF1157 BAF1157 AF5378 AF2570 BAF2570 MAB2570 AF3385 BAF3385 AF5056 BAM1547 AF1850 MAB1850 AF2444 BAF2444 MAB2444 AF5857 PPS080 PPS082 PPS081 PPS083 PPS012 PPS013 PPS033 MAB167 AF719 AF3098
100 g 100 g 50 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 50 g 100 g 100 g 50 g 100 g 100 g 50 g 100 g 100 g 100 g 500 g 100 g 50 g 100 g 100 g 25 g 25 g 25 g 25 g 10 g 10 g 10 g 500 g 100 g 100 g
$355 $355 $380 $355 $355 $355 $240 $240 $355 $355 $240 $355 $380 $355 $355 $380 $240 $355 $380 $355 $380 $355 $325 $355 $380 $240 $355 $355 $355 $355 $355 $355 $355 $355 $325 $355 $355
Neurturin NF2/Merlin aa 363-578 NFB2 aa 1-447 NG2/MCSP b-NGF
NGF R/TNFRSF16
Mouse Human
Nicastrin Isoform 1
Nidogen-1/Entactin
Human
Biotin None
Nidogen-2 Ninjurin-2 NKG2D/CD314 NKp46/NCR1
Human Human Mouse Human
None Biotin None Biotin None None None
NKX2.5
Human
Biotin None
NKX6.1 NMDA R, NR1 Subunit C1 Splice Variant NMDA R, NR1 Subunit C2 Splice Variant NMDA R, NR1 Subunit N1 Splice Variant NMDA R, NR2A Subunit NMDA R, NR2B Subunit NMDA R, NR2C Subunit N-Me-6,7-diOH-TIQ Noggin Nogo-A aa 2-172
Human/Mouse Mouse/Rat Human/Mouse/Rat Mouse/Rat Mouse/Rat Human/Mouse/Rat Human/Mouse/Rat Human/Mouse/Rat Multispecies Mouse Rat
80

MOLECULE Nogo-A aa 566-748 SPECIES Human LABEL None CLONE Polyclonal HOST/TYPE Sheep IgG CATALOG # AF3515 SIZE 100 g PRICE $355
Nogo-A in differentiated rat cortical stem cells. Nogo-A was detected in 7-day differentiated rat cortical stem cells using anti-human Nogo-A affinitypurified polyclonal antibody (Catalog # AF3515). Cells were stained using NorthernLights-557 donkey anti-sheep IgG secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue).
Nogo-C Norrin eNOS aa 1030-1203 iNOS aa 781-798 nNOS Notch-1 Notch-1 Intracellular Domain, aa 2428-2556
Human Human Human Human Human Rat Human
None None None Biotin None None None None None
499825 Polyclonal Polyclonal Polyclonal 2D2-B2 Polyclonal Polyclonal 433802 Polyclonal
Mouse IgG3 Goat IgG Goat IgG Goat IgG Mouse IgG1 Goat IgG Goat IgG Mouse IgG2A Sheep IgG
MAB5766 AF3014 AF950 BAF950 MAB9502 AF2416 AF1057 MAB3647 AF3647
100 g 100 g 100 g 50 g 100 g 100 g 100 g 100 g 100 g
$240 $355 $355 $380 $240 $355 $355 $240 $355
Notch-1 in Human SAOS-2 Cells. Notch-1 was detected in human SAOS-2 osteosarcoma cells using anti-human Notch-1 affinity-purified polyclonal antibody (Catalog # AF3647). Cells were stained using NorthernLights-557 donkey anti-sheep IgG secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue).
Notch-2
Rat
None
Polyclonal
Goat IgG
AF1190
100 g
$355
Notch-2 in Rat Brain. Notch-2 was detected in cryostat sections of rat brain (choroid plexus) using anti-rat Notch-2 affinity-purified polyclonal antibody (Catalog # AF1190). Tissues were stained using antigoat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Notch-2 Intracellular Domain Notch-3
Human Mouse
None None Biotin None None None Biotin None None
Polyclonal Polyclonal Polyclonal Polyclonal 411913 Polyclonal Polyclonal 353124 Polyclonal
Goat IgG Goat IgG Goat IgG Goat IgG Rat IgG2A Goat IgG Goat IgG Mouse IgG2B Goat IgG
AF3735 AF1308 BAF1308 AF3847 MAB3847 AF1640 BAF1640 MAB3835 AF2034
100 g 100 g 50 g 100 g 100 g 100 g 50 g 100 g 100 g
$355 $355 $380 $355 $240 $355 $380 $240 $355
Notch-4 Intracellular Domain
Human
NOV/CCN3 NRAGE NrCAM Isoform 3
Human Human Human
81

MOLECULE NT-3 SPECIES Human LABEL None CLONE Polyclonal HOST/TYPE Goat IgG CATALOG # AF-267-NA SIZE 100 g PRICE $355
NT-3 in Rat Cerebellum. Neurotrophin-3 (NT-3) was detected in a cryostat section of rat cerebellar Purkinje neurons using anti-human NT-3 affinitypurified polyclonal antibody (Catalog # AF-267-NA). Tissues were stained with AEC substrate (red) and counterstained with hematoxylin (blue).
NT-4 Nucleostemin
Human Human/Mouse/Rat
None None
Goat IgG Goat IgG
AF-268-NA AF1638
100 g 100 g
$355 $355
Nucleostemin in Human U2OS Cells. Nucleostemin was detected in the nucleoli of the U2OS human osteosarcoma cell line using anti-human/mouse/ rat Nucleostemin affinity-purified polyclonal antibody (Catalog # AF1638). Cells were stained using NorthernLights-557 anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).
None Numb Human Biotin None Nurr-1/NR4A2 N-WASP OAS2 OBCAM Human Human/Mouse Human Human Human Human Human/Mouse None None None None None None Biotin None
Polyclonal Polyclonal 447716 N1404 Polyclonal Polyclonal HLS56/3 Polyclonal Polyclonal Polyclonal 240408
Sheep IgG Sheep IgG Mouse IgG2A Mouse IgG2A Goat IgG Goat IgG Mouse N/A Goat IgG Goat IgG Goat IgG Rat IgG2B
AF4338 BAF4338 MAB4338 PP-N1404-00 AF2156 AF3854 MAB1925 AF2777 AF1759 BAF1759 MAB1759
100 g 50 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 50 g 100 g
$355 $380 $240 $380 $355 $355 $240 $355 $355 $380 $240
Oct-3/4
Oct-3/4 and Alkaline Phosphatase in Human BG01V Cells. Oct-3/4 was detected in BG01V embryonic stem cells using anti-human Oct-3/4 affinity-purified polyclonal antibody (Catalog # AF1759). Alkaline phosphatase (ALPL) was detected using anti-human/mouse/rat ALPL monoclonal antibody (Catalog # MAB1448). Cells were stained for Oct-3/4 using NorthernLights-637 donkey antigoat IgG secondary antibody (Catalog # NL002; red) and stained for ALPL using NorthernLights-557 donkey anti-mouse IgG secondary antibody (pseudo-stained green; Catalog # NL007).
Oct-3/4 NorthernLights-conjugated Olig1 Olig 1, 2, 3
Human/Mouse Human Human
NL493 None None None
240408 Polyclonal 257219 257224
Rat IgG2B Goat IgG Mouse IgG2B Mouse IgG2A
NL1759G AF2417 MAB2417 MAB2230
500 L 100 g 100 g 100 g
$345 $355 $240 $295
82

MOLECULE Olig2 SPECIES Human LABEL None Biotin CLONE Polyclonal Polyclonal HOST/TYPE Goat IgG Goat IgG CATALOG # AF2418 BAF2418 SIZE 100 g 50 g PRICE $355 $380
Olig2 and Oligodendrocyte Marker O4 in Rat Cortical Stem Cells (RCSC). Olig2 and O4 were detected in 7 day differentiated RCSC using anti-human Olig2 affinity-purified polyclonal antibody (Catalog # AF2418) and anti-human/mouse/rat/chicken O4 monoclonal antibody (Catalog # MAB1326). Cells were stained for Olig2 using NorthernLights-637 anti-goat IgG secondary antibody (Catalog # NL002; red) and for O4 using an anti-mouse IgM Secondary antibody (green).
Olig3
Human Human/Mouse
None Biotin None None None
Polyclonal Polyclonal 257934 O1 O4
Goat IgG Goat IgG Mouse IgG1 Mouse IgM Mouse IgM
AF2356 BAF2356 MAB2456 MAB1327 MAB1326
100 g 50 g 100 g 50 g 50 g
$355 $380 $240 $200 $2040
Oligodendrocyte Marker O1 Oligodendrocyte Marker O4
Human/Mouse/Rat/Chicken Human/Mouse/Rat/Chicken
Oligodendrocyte Marker O4 in Rat Cortical Stem Cells (RCSC). Oligodendrocyte marker O4 (O4) was detected in 7-day differentiated RCSC using anti-human/mouse/rat/chicken O4 monoclonal antibody (Catalog # MAB1326). Cells were stained using NorthernLights-557 donkey anti-mouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).
Oligodendrocyte Marker O4 NorthernLights-conjugated Orexin A Orexin B
Human/Mouse/Rat/Chicken Human/Mouse/Rat Human
NL637 Biotin None None
O4 97505 97505 145202
Mouse IgM Mouse IgG1 Mouse IgG1 Mouse IgG1
NL1326V BAM763 MAB763 MAB734
500 L 250 g 500 g 500 g
$345 $380 $325 $325
Orexin B, p38a, & Integrin b1 in Mouse Brain Stem. Orexin B, p38a, and Integrin b 1 were detected in frozen sections of mouse brain stem using antihuman Orexin B monoclonal antibody (Catalog # MAB734), anti-human/mouse/rat p38a affinity-purified polyclonal antibody (Catalog # AF8691), and anti-mouse Integrin b1 affinity-purified polyclonal antibody (Catalog # AF2405). Tissue was stained with NorthernLights (NL) secondary polyclonal antibodies: NL-493 anti-rabbit IgG (Catalog # NL006; green), NL-557 anti-mouse IgG (Catalog # NL007; red), and NL-637 anti-goat IgG (Catalog # NL002; blue).
Oncostatin M/OSM
Mouse Human
None None None
Polyclonal 469221 Polyclonal 118125 118125 303822 190125 Polyclonal Polyclonal 190312 Polyclonal Polyclonal Polyclonal Polyclonal
Goat IgG Mouse IgG1 Goat IgG Rat IgG2A Rat IgG2A Mouse IgG2B Mouse IgG1 Goat IgG Goat IgG Mouse IgG2B Goat IgG Goat IgG Goat IgG Goat IgG
AF-495-NA MAB4389 AF662 BAM662 MAB662 MAB25501 MAB1419 AF1433 BAF1433 MAB1433 AF808 AF805 BAF805 BAF459
100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 50 g 100 g 100 g 100 g 50 g 50 g
$355 $240 $355 $240 $240 $240 $240 $355 $380 $240 $355 $355 $380 $380
OSM Rb
Mouse
Biotin None
Osteoactivin/GPNMB Osteocalcin
Human Human
None None None
Osteopontin/OPN
Human
Biotin None
Mouse Human Mouse
None None Biotin Biotin
Osteoprotegerin/TNFRSF11B
83

MOLECULE SPECIES LABEL None Otx2 Human Biotin None OV-6 OX40 Ligand/TNFSF4 Human/Rat Human Mouse None None None None p21/CIP1/CDKN1A Human Biotin None
p21 in Human Breast Cancer. p21 was detected in paraffin-embedded human breast cancer tissue sections using anti-human p21 affinity-purified polyclonal antibody (Catalog # AF1047). Tissues were stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with haematoxylin (blue).
CLONE Polyclonal Polyclonal 246826 OV-6 159403 Polyclonal Polyclonal Polyclonal 195720
HOST/TYPE Goat IgG Goat IgG Mouse IgG2B Mouse IgG1 Mouse IgG1 Goat IgG Goat IgG Goat IgG Mouse IgG2A
CATALOG # AF1979 BAF1979 MAB1979 MAB2020 MAB10541 AF1236 AF1047 BAF1047 MAB1047
SIZE 100 g 50 g 100 g 100 g 500 g 100 g 100 g 50 g 200 g
PRICE $355 $380 $240 $295 $325 $355 $355 $380 $240
p27/Kip1 Phospho-p38 MAP Kinase (T180/Y182)
Human Human/Mouse/Rat Human/Mouse/Rat
None None None
Polyclonal 341116 Polyclonal Rat IgG2A Rabbit IgG
CTS2256 MAB22561 AF869
50 Tests 100 g 100 g
$425 $240 $285
Phosphorylated p38 in Embryonic Mouse Eye. Phosphorylated p38 was detected in a frozen section of E15 mouse eye cup using anti-phospho-p38 (T180/Y182) affinity-purified polyclonal antibody (Catalog # AF869). The tissue was stained using antirabbit HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS005; brown) and counterstained with hematoxylin (blue).
p38a p38b p38g p38d Phospho-p53 (S15) Phospho-p53 (S46)
Human/Mouse/Rat Human Human/Mouse/Rat Human Human Human
Polyclonal 142102 280071 Polyclonal Polyclonal Polyclonal 261366 Polyclonal Polyclonal Polyclonal 241631 Polyclonal Polyclonal 215247 Polyclonal
Rabbit IgG Mouse IgG2B Mouse IgG2B Rabbit IgG Rabbit IgG Rabbit IgG Mouse IgG1 Rabbit IgG Goat IgG Goat IgG Mouse IgG2B Rabbit IgG Goat IgG Mouse IgG1 Goat IgG
AF8691 MAB869 MAB3274 AF1347 AF1519 AF1043 MAB18391 AF1489 AF1916 BAF1916 MAB1916 AF8962 AF8964 MAB8962 AF896
$285 $240 $240 $285 $285 $285 $240 $285 $355 $380 $240 $285 $355 $240 $285
p63/TP73L
Human
Biotin None None
p70 S6 Kinase
Human/Mouse/Rat
None None
Phospho-p70 S6 Kinase (T229)
Human
None
84

MOLECULE PAK7 SPECIES Human/Rat LABEL None CLONE 444703 HOST/TYPE Mouse IgG2B CATALOG # MAB4696 SIZE 100 g PRICE $240
PAK7 in Human Cerebellum. p21-activated kinase 7 (PAK7) was detected in a paraffin-embedded human cerebellum section using anti-human PAK7 monoclonal antibody (Catalog # MAB4696). Tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).
Park7/DJ-1 Parkin PARP Parvalbumin a
Human Human Multi-species Human/Mouse/Rat
None None None None
Polyclonal Polyclonal C2-10 Polyclonal
Goat IgG Goat IgG Mouse IgG1 Sheep IgG
AF3995 AF1438 4338-MC-50 AF5058
100 g 100 g 50 g 100 g
$355 $355 $195 $355
Parvalbumin a in Human Cortex. Parvalbumin a was detected in paraffin-embedded sections of human cortex using anti-human/mouse/rat Parvalbumin a monoclonal antibody (Catalog # AF5058). Tissue was stained using anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).
Patched (First Extracellular Loop)
Mouse
None
413220
Rat IgG2A
MAB41051
100 g
$240
Patched in Embryonic Mouse Spinal Cord. Patched was detected in ventral E13.5 mouse spinal cord using anti-mouse Patched monoclonal antibody (Catalog # MAB41051; red). Tissue was counterstained with DAPI (blue).
Patched 2 (First Extracellular Loop) Pax3 Pax4 Pax5 Pax6 Pax7 PBR
Human Human Human/Mouse Human Human Mouse/Rat/Chicken Human/Mouse/Rat/Chicken Human/Mouse/Rat Mouse/Rat Human
None None None None None Biotin None None None None None None Biotin None Biotin None None None None None
Polyclonal Polyclonal 274212 Polyclonal Polyclonal PAX6 PAX6 PAX7 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal
Goat IgG Goat IgG Mouse IgG2A Goat IgG Goat IgG Mouse IgG1 Mouse IgG1 Mouse IgG1 Rabbit IgG Rabbit IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Total Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG
AF4078 AF2457 MAB2457 AF2614 AF3487 BAM1260 MAB1260 MAB1675 6361-PC-100 6362-PC-100 AF1086 AF1021 BAF1021 AF1143 BAF1143 AB-221-NA AF-221-NA AF1055 AF1560 AF1447
100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 L 100 L 100 g 100 g 50 g 100 g 50 g 1 mg 100 g 100 g 100 g 100 g
$355 $355 $240 $355 $355 $260 $240 $240 $310 $310 $355 $355 $380 $355 $380 $355 $355 $355 $355 $355
PD-1
Mouse
PD-ECGF
Human
PDGF-AA
Human Rat
PDGF-C
Human Mouse
85

MOLECULE PDGF-D SPECIES Human Human PDGF Ra Mouse Phospho-PDGF Ra (Y742) Phospho-PDGF Ra (Y762) Human Human Human PDGF Rb Mouse LABEL None None None None Biotin None None None None None Biotin CLONE Polyclonal Polyclonal 35248 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal PR7212 Polyclonal Polyclonal HOST/TYPE Goat IgG Goat IgG Mouse IgG1 Goat IgG Goat IgG Rabbit IgG Rabbit IgG Goat IgG Mouse IgG1 Goat IgG Goat IgG CATALOG # AF1159 AF-307-NA MAB322 AF1062 BAF1062 AF2114 AF21141 AF385 MAB1263 AF1042 BAF1042 SIZE 100 g 100 g 500 g 100 g 50 g 50 g 50 g 100 g 250 g 100 g 50 g PRICE $355 $355 $325 $355 $380 $285 $285 $355 $230 $355 $380
PDGF Rb in Mouse Embryo. Platelet-derived Growth Factor Receptor b (PDGF Rb) was detected in a cryostat tissue section of E13 mouse spinal cord using anti-mouse PDGF Rb affinity-purified polyclonal antibody (Catalog # AF1042). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Human PD-L2 Mouse
None None None Biotin None Biotin None None None None None None None None None None None None None None None None None None
Polyclonal 176611 Polyclonal Polyclonal Polyclonal Polyclonal 267712 Polyclonal Polyclonal Polyclonal 345613 Polyclonal 275508 Polyclonal Polyclonal Polyclonal 257417 420920 Polyclonal Polyclonal HL71/10 Polyclonal 346404 408305
Goat IgG Mouse IgG2B Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG2B Sheep IgG Sheep IgG Goat IgG Rat IgG2A Goat IgG Mouse IgG2B Sheep IgG Goat IgG Goat IgG Mouse IgG2B Rat IgG2A Goat IgG Sheep IgG Mouse IgG1 Goat IgG Mouse IgG1 Rat IgG2A
AF1224 MAB1224 AF1022 BAF1022 AF2419 BAF2419 MAB2419 AF4997 AF5365 AF4565 MAB3548 AF2955 MAB2388 AF5559 AF2800 AF877 MAB2294 MAB4357 AF3759 AF4465 MAB1980 AF742 MAB3716 MAB4309
100 g 500 g 100 g 50 g 100 g 50 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g
$355 $325 $355 $380 $355 $380 $240 $355 $355 $355 $240 $355 $240 $355 $355 $355 $240 $240 $355 $355 $295 $355 $240 $240
PDX-1/IPF1
Human Human/Mouse
PDZK1 PEDF R PER1 aa 159-365 Periostin/OSF-2 Periostin/OSF-2 Isoform 2 Persephin PIBF1 pIgR PIN Pin1 aa 2-163 PINK1 PKCb1 PKC/ PKC // Cross-reactive, aa 446-587 PKR Plasminogen Kringle 5 PLC-g2 aa 400-600 Plexin A1
Human/Mouse Human/Mouse/Rat Human Mouse Mouse Human Human Mouse Human Human/Mouse Human Human/Rat Human/Mouse/Rat Human Mouse Human/Mouse Mouse
86

MOLECULE Plexin A3 SPECIES Mouse LABEL None CLONE Polyclonal HOST/TYPE Goat IgG CATALOG # AF4075 SIZE 100 g PRICE $355
Plexin A3 Expression in Embryonic Spinal Cord. Plexin A3 was detected in mouse embryonic (E13.5) spinal cord using anti-mouse Plexin A3 affinity-purified polyclonal antibody (Catalog # AF4075). Cells were stained using a NorthernLights-557 secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).
Plexin B1 Plexin B2
Human Human
None None None
Goat IgG Sheep IgG Mouse IgG2A
AF3749 AF5329 MAB5329
100 g 100 g 100 g
$355 $355 $240
Plexin B2 in Embryonic Mouse Choroid Plexus. Plexin B2 was detected in a section of embryonic (E13.5) mouse choroid plexus using anti-human Plexin B2 affinity-purified polyclonal antibody (Catalog # AF5329). The tissue was stained using antisheep NorthernLights-557 secondary antibody (Catalog # NL010; red) and the nuclei counterstained with DAPI (blue).
Plexin B2 in Human Colon Cancer. Plexin B2 was detected in paraffin-embedded human colon cancer sections using anti-human Plexin B2 affinity-purified polyclonal antibody (Catalog # AF5329). The tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013) and stained using the anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown). The tissue was counterstained with hematoxylin (blue).
PNR/NR2E3
Human
None None
H7223 Polyclonal Polyclonal 222328 Polyclonal Polyclonal 192703
Mouse IgG2A Goat IgG Goat IgG Mouse IgG2A Goat IgG Goat IgG Rat IgG2B
PP-H7223-00 AF1658 BAF1658 MAB1658 AF1556 BAF1556 MAB1556
100 g 100 g 50 g 100 g 100 g 50 g 100 g
$380 $355 $380 $240 $355 $380 $240
Human Podocalyxin Mouse
Biotin None None Biotin None
Podocalyxin in Human Kidney. Podocalyxin was detected in a paraffin-embedded human kidney tissue section using anti-human Podocalyxin affinity-purified polyclonal antibody (Catalog # AF1658). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Podocalyxin in Human Embryonic Stem Cells (BGO1V). Podocalyxin was detected in the BGO1V human embryonic stem cell line cultured on mouse embryonic fibroblasts using anti-human Podocalyxin monoclonal antibody (Catalog # MAB1658). The cells were stained with anti-mouse NorthernLights-557 secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).
Podocalyxin NorthernLights-conjugated Podocan
Human Human Mouse Human Mouse Rat/Xenopus Mouse/Rat Human Human Human Human
NL557 None None None None None None None None None None Biotin None None Biotin
222328 390117 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 234532 528322 A3409A Polyclonal Polyclonal Polyclonal 121121 Polyclonal
Mouse IgG2A Mouse IgG2B Goat IgG Sheep IgG Goat IgG Rabbit IgG Rabbit IgG Rat IgG2B Mouse IgG1 Mouse IgG2A Goat IgG Goat IgG Goat IgG Mouse IgG2B Goat IgG
NL1658R MAB42201 AF3104 AF3670 AF3244 PPS015 PPS016 MAB16531 MAB5595 PP-A3409A-00 AF166 BAF166 AF149 MAB149 BAF197
500 L 100 g 100 g 100 g 100 g 100 L 100 L 100 g 100 g 100 g 100 g 50 g 100 g 500 g 50 g
$345 $240 $355 $355 $355 $355 $355 $240 $240 $380 $355 $380 $355 $325 $380
Podoplanin Potassium Channel Kv2.2 Phospho-Potassium Channel Kv3.1 (S503) PP2A Catalytic Subunit, aa 209-309 PP2Cg/PPM1G PPARg/NR1C3 Presenilin-1 C-Terminal Fragment, aa 298-407
Presenilin-1 N-Terminal Fragment, aa 1- 80 Presenilin-2 C-Terminal Fragment, aa 322-388
Human Human
87

MOLECULE Presenilin-2 N-Terminal Fragment, aa 1- 80 PRL/PTP4A Pan Specific SPECIES Human Human Human Human/Mouse/Rat/Primate Progesterone R/NR3C3 B Human LABEL None None None None None None CLONE Polyclonal 334407 A9621A 1154 608 H5344 HOST/TYPE Goat IgG Mouse IgG2B Mouse IgG2A Mouse IgG1 Mouse IgG1 Mouse IgG2A CATALOG # AF153 MAB32191 PP-A9621A-00 PPS017 PPS018 PP-H5344-00 SIZE 100 g 100 g 100 g 100 L 100 L 100 g PRICE $355 $240 $380 $355 $355 $380
Progesterone R/NR3C3
Progesterone Receptor-B/NR3C3 in Human Endometrium. Progesterone Receptor isoform B was detected in paraffin-embedded human endometrial epithelial cells using anti-human Progesterone Receptor isoform B monoclonal antibody (Catalog # PPH5344-00). Tissues were stained with a 0.01% DAB solution (brown) and counterstained with hematoxylin (blue).
Human Progranulin Mouse Human Mouse Human Rat Human Human
None None None None None Biotin None None None None
Polyclonal Polyclonal 333731 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 244029 Polyclonal
Goat IgG Sheep IgG Rat IgG2A Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG2B Sheep IgG
AF2420 AF2557 MAB25571 AF3470 AF1445 BAF1445 AF1167 AF1112 MAB2024 AF4599
100 g 100 g 100 g 100 g 100 g 50 g 100 g 100 g 100 g 100 g
$355 $355 $240 $355 $355 $380 $355 $355 $295 $355
Prohibitin Prolactin
Prolactin R Prominin 2 Prostasin/Prss8
Prostasin in Human Prostate Cancer. Prostasin was detected in a paraffin-embedded human prostate cancer section using anti-human Prostasin affinity-purified polyclonal antibody (Catalog # AF4599). The tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013). The section was stained using the antisheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).
Protocadherin a1
Human
None
Polyclonal
Sheep IgG
AF5064
100 g
$355
Protocadherin a1 in Mouse Cerebellum. Protocadherin a1 was detected in a section of adult mouse cerebellum using anti-mouse affinity-purified polyclonal antibody (Catalog # AF5064). The tissue was stained using anti-sheep NorthernLights-557 secondary antibody (Catalog # NL010; red) and the nuclei counterstained with DAPI (blue).
Protocadherin-1
Human
None
Polyclonal
Goat IgG
AF5899
100 g
$355
88

MOLECULE Protogenin SPECIES Mouse LABEL None CLONE Polyclonal HOST/TYPE Sheep IgG CATALOG # AF4919 SIZE 100 g PRICE $355
Protogenin in Embryonic Neural Tube. Protogenin (PRTG) was detected in a section of E9 mouse neural tube using anti-mouse PRTG affinity-purified polyclonal antibody (Catalog # AF4919). The tissue was stained using NorthernLights-557 anti-sheep polyclonal antibody (Catalog # NL101; red) and counterstained with DAPI (blue).
Prox1 PSMA/FOLH1/NAALADase I PTEN PTEN aa 385-403 PTH1 R PTP1B PTP b/ aa 302-754 PTP-MEG2 PTPRg aa 111-541
Human Human Human/Mouse/Rat Human/Mouse/Rat Human Human Human Human/Mouse/Rat Human
Polyclonal Polyclonal 217702 Polyclonal Polyclonal Polyclonal 279245 291835 Polyclonal
Goat IgG Sheep IgG Mouse IgG1 Rabbit IgG Sheep IgG Rabbit IgG Rat IgG2A Mouse IgG2B Goat IgG
AF2727 AF4234 MAB847 AF847 AF5709 AF1366 MAB26881 MAB2668 AF4608
100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g
$355 $355 $240 $285 $355 $285 $240 $240 $355
PTPRg in Human Kidney. Protein Tyrosine Phosphatase, Receptor type g (PTPRg) was detected in paraffin-embedded normal human kidney tissue sections using anti-human PTPRg affinity-purified polyclonal antibody (Catalog # AF4608). Tissues were stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
PTPR aa 30-1260 RACK1 Rae-1g
Human Human/Mouse/Rat Mouse Human
Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 175410
Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Rat IgG2A
AF3430 AF3434 AF1136 AF1145 AF1179 MAB1179
100 g 100 g 100 g 100 g 100 g 500 g
$355 $355 $355 $355 $355 $325
RAGE
Mouse Mouse/Rat
RAGE Expression in Human Alzheimers Brain. Receptor for Advanced Glycation End products (RAGE) was detected in paraffin-embedded Alzheimers disease human cerebellum sections using anti-human RAGE affinity-purified polyclonal antibody (Catalog # AF1145). Tissue was stained using the anti-goat HRPDAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
RANK/TNFRSF11A Rap1A/B RARRES1 Reelin Reg II Reg 2/PAP
Human Mouse Human/Mouse/Rat Mouse Mouse Mouse Rat
80707 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 293523
Mouse IgG2A Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG1
MAB6831 AF692 AF3767 AF4657 AF3820 AF2035 MAB1996
500 g 100 g 100 g 100 g 100 g 100 g 100 g
$325 $355 $355 $355 $355 $355 $240
89

MOLECULE Reg IIIb SPECIES Mouse LABEL None CLONE Polyclonal HOST/TYPE Sheep IgG CATALOG # AF5110 SIZE 100 g PRICE $355
Reg IIIb in Mouse Small Intestine. Reg IIIb was detected in adult mouse small intestine sections using anti-mouse Reg IIIb affinity-purified polyclonal antibody (Catalog # AF5110). Cells were stained using NorthernLights-557 donkey anti-sheep IgG secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue).
Reg IIId Reg IV RelB aa 382-579
Mouse Human Human
None None None
Rat IgG2A Goat IgG Mouse IgG2B
MAB5678 AF1379 MAB2698
100 g 100 g 100 g
$240 $355 $240
RelB in Human Lymphoma. RelB was detected in a paraffin-embedded human lymphoma section using anti-human RelB monoclonal antibody (Catalog # MAB2698). Tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).
c-Rel c-Rel aa 1-588
Human Human/Mouse Mouse
None None Biotin
Rat IgG2A Goat IgG Rat IgG2A
MAB4606 AF2699 BAM2699
50 g 100 g 100 g
$240 $355 $380
c-Rel in Mouse Splenocytes. c-Rel was detected in mouse splenocytes using anti-mouse c-Rel biotinylated monoclonal antibody (Catalog # BAM2699).Cells were stained using NorthernLights Streptavidin-557 (Catalog # NL999; red) and counterstained with DAPI (blue)..
Renin Renin 1 Renin 1 Propeptide Renin Propeptide Resistin Ret REVERBb/NR1D2
Human Mouse Mouse Human Mouse Human Mouse Human
Polyclonal Polyclonal 434703 411507 Polyclonal Polyclonal Polyclonal H2729 Polyclonal Polyclonal 373722
Sheep IgG Goat IgG Rat IgG2A Mouse IgG1 Goat IgG Goat IgG Goat IgG Mouse IgG3 Sheep IgG Sheep IgG Rat IgG2A
AF4090 AF4277 MAB42771 MAB4447 AF1069 AF1485 AF482 PP-H2729-00 AF3598 BAF3598 MAB3598
100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 50 g 100 g
$355 $355 $240 $240 $355 $355 $355 $380 $355 $380 $240
Rex-1
Human
Biotin None
Rex-1 in Human NTera2 Cells. Rex-1 was detected in human NTera2 embryonic carcinoma cell line using antihuman monoclonal antibody (Catalog # MAB3598). Cells were stained using NorthernLights-557 goat antirat IgG secondary antibody (Catalog # NL013; red) and counterstained with DAPI (blue).
90

MOLECULE SPECIES Human Repulsive Guidance Molecule A/RGM-A Mouse Human/Mouse Mouse LABEL None None Biotin None Biotin CLONE Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal HOST/TYPE Goat IgG Goat IgG Goat IgG Sheep IgG Sheep IgG CATALOG # AF2459 AF2458 BAF2458 AF3597 BAF3597 SIZE 100 g 100 g 50 g 100 g 50 g PRICE $355 $355 $380 $355 $380
RGM-B
RGM-B in Mouse Brain. Repulsive Guidance Molecule B (RGM-B) was detected in a cryostat section of mouse brain (trigeminal ganglia) using anti-mouse RGM-B affinity-purified polyclonal antibody (Catalog # AF3597). Tissues were stained using anti-sheep HRP-DAB detection (brown) and counterstained with hematoxylin (blue).
Rheb Phospho-Ribosomal Protein S6 (S235/S236)
Human/Mouse/Rat Human/Mouse/Rat
None None
344912 Polyclonal
Mouse IgG2B Rabbit IgG
MAB3426 AF3918
100 g 100 g
$240 $285
Phospho-RPS6 in Human Colon Cancer. PhosphoRibosomal Protein S6 (S235/S236) was detected in paraffin-embedded human colon cancer tissue sections using anti-human/mouse/rat affinity-purified phospho-RPS6 (S235/S236) affinity-purified polyclonal antibody (Catalog # AF3918). The tissue was stained using anti-rabbit HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS005; brown) and counterstained with hematoxylin (blue).
ROBO1 ROBO2
Rat Human
None None None None None None None None None None None None None None None None None None None
Polyclonal Polyclonal 356001 Polyclonal 327111 H3925 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 232902 Polyclonal K8508 K8508 Polyclonal Polyclonal 438709
Goat IgG Goat IgG Mouse IgG1 Goat IgG Mouse IgG2B Mouse IgG2A Rabbit IgG Rabbit IgG Rabbit IgG Rabbit IgG Rabbit IgG Goat IgG Rat IgG2B Goat IgG Mouse IgG2A Mouse IgG2A Sheep IgG Goat IgG Rat IgG2B
AF1749 AF3147 MAB3147 AF3076 MAB3076 PP-H3925-00 AF889 AF892 AF992 AF1518 AF893 AF2006 MAB2006 AF3765 PP-K8508-00 PP-K8508-10 AF4649 AF4870 MAB4138
$355 $355 $240 $355 $240 $380 $285 $285 $285 $285 $285 $355 $240 $355 $380 $380 $355 $355 $240
ROBO3 ROR/NR1F1-3 Pan Specific Phospho-RSK (S380) Pan Specific Phospho-RSK1/RSK2 (RSK1 S221, RSK2 S227) RSK1 RSK2 Phospho-RSK3 (S218) RUNX2/CBFA1 RUNX3/CBFA3 aa 186-415 RXRa/NR2B1 Ryk S100A2 S100A4
Human Human Human/Mouse/Rat Human/Mouse/Rat Human/Mouse/Rat Human/Mouse/Rat Human/Mouse Human Human/Mouse Human Human Mouse Human Mouse
91

MOLECULE SPECIES LABEL None S100A8 Mouse Biotin None
S100A8 in Mouse NIH-3T3 Cells. S100A8 was detected in mouse NIH-3T3 embryonic fibroblast cell line using anti-mouse S100A8 monoclonal antibody (Catalog # MAB3059; red). Cells were counterstained with DAPI (blue).
CLONE Polyclonal Polyclonal 335806
HOST/TYPE Goat IgG Goat IgG Rat IgG2B
CATALOG # AF3059 BAF3059 MAB3059
SIZE 100 g 50 g 100 g
PRICE $355 $380 $240
S100A9
Human Mouse
None None
474315 Polyclonal
Mouse IgG2B Goat IgG
MAB5578 AF2065
100 g 100 g
$240 $355
S100A9 in Mouse XB2 Cells. S100A9 was detected in mouse XB2 teratoma cell line using anti-mouse S100A9 affinity-purified polyclonal antibody (Catalog # AF2065). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).
Human S100A10
None None
Polyclonal Polyclonal Polyclonal 344015 Polyclonal Polyclonal
Goat IgG Goat IgG Goat IgG Rat IgG2A Goat IgG Goat IgG
AF1698 AF2377 BAF2377 MAB2377 AF4874 AF4328
100 g 100 g 50 g 100 g 100 g 100 g
$355 $355 $380 $240 $355 $355
Mouse
Biotin None
S100A11 S100A13
Human Mouse
None None
S100A13 in Embryonic Mouse Cartilage. S100A13 was detected in a cryostat section of developing embryonic mouse (E13) cartilage using anti-mouse S100A13 affinity-purified polyclonal antibody (Catalog # AF4328). Tissues were stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
S100A16
Human
None
Polyclonal
Sheep IgG
AF5180
100 g
$355
S100A16 in Human Brain Cortex. S100A16 was detected in a paraffin-embedded human brain cortex section using anti-human S100A16 affinity-purified polyclonal antibody (Catalog # AF5180). The tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013). The section was stained using the anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).
S100B
Human
None
Polyclonal
Goat IgG
AF1820
100 g
$355
92

MOLECULE S100P SPECIES Human LABEL None None CLONE Polyclonal 357517 HOST/TYPE Goat IgG Mouse IgG1 CATALOG # AF2957 MAB2957 SIZE 100 g 100 g PRICE $355 $240
S100P in Human Liver. S100P was detected in paraffin-embedded human liver sections using antihuman S100P affinity-purified polyclonal antibody (Catalog # AF2957). Tissue was stained using antigoat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
SALL1 aa 258-499 d-Sarcoglycan
Human Human
None None None
K9814 Polyclonal Polyclonal Polyclonal 177228 177228 462002 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 180627 180627
Mouse IgG2A Goat IgG Goat IgG Goat IgG Rat IgG2A Rat IgG2A Rat IgG2A Goat IgG Goat IgG Rabbit IgG Goat IgG Goat IgG Goat IgG Rat IgG2A Rat IgG2A
PP-K9814-00 AF2778 AF1226 BAF1226 BAM1226 MAB1226 MAB4754 AF2278 AF2268 AF3527 AF332 AF1356 BAF1356 BAM1356 MAB1356
$380 $355 $355 $380 $380 $325 $240 $355 $355 $285 $355 $355 $380 $380 $325
Sca-1/Ly6
Mouse
Biotin Biotin None
SCARA5 SCF/c-kit Ligand Phospho-SCF R/c-kit (Y730)
Mouse Canine Feline Human Human
SCF R/c-kit
Mouse
Biotin Biotin None
SCF R in Mouse Cerebellum. Stem Cell Factor Receptor (SCF R) was detected in frozen sections of mouse cerebellum using anti-mouse SCF R monoclonal antibody (Catalog # MAB1356). Tissue was stained using anti-rat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS017; brown) and counterstained with hematoxylin (blue).
SCF R/c-kit aa 716-726 SCGF/CLEC11a SCL/Tal1 SCL/Tal1
Polyclonal 239029 Polyclonal 333803 294301 Polyclonal Polyclonal 300812 BBIG-E6 BBIG-E4 Polyclonal BBIG-E4 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal
Rabbit IgG Mouse IgG2A Goat IgG Mouse IgG2B Mouse IgG2B Goat IgG Goat IgG Rat IgG2A Mouse IgG1 Mouse IgG1 Goat Serum Mouse IgG1 Chicken IgY Chicken IgY Goat IgG Goat IgG Goat IgG
AF3267 MAB1904 AF3360 MAB3360 MAB2357 AF2556 BAF2556 MAB2556 BBA1 BBA16 BBA18 BBA2 AF575 BAF575 AF977 AF1534 BAF1534
50 g 500 g 100 g 100 g 100 g 100 g 50 g 100 g 100 g 200 g 500 L 1 mg 100 g 50 g 100 g 100 g 50 g
$285 $325 $355 $240 $240 $355 $380 $240 $230 $230 $195 $840 $355 $380 $355 $355 $380
SDNSF/MCFD2
Mouse
Biotin None None
Human E-Selectin/CD62E Mouse Rat L-Selectin/CD62L Rat
None None None None Biotin None None Biotin
93

MOLECULE P-Selectin/CD62P Semaphorin 3C Semaphorin 3E Semaphorin 4D/CD100 SPECIES Human Mouse Human Mouse Human Semaphorin 6A Mouse Human Mouse Human Mouse Human Human Human LABEL None None Biotin None None None None None None None None None None None None None None None Biotin None None CLONE 9.00E+01 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 201932 Polyclonal Polyclonal 256631 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 187003 Polyclonal Polyclonal Polyclonal Polyclonal HOST/TYPE Mouse IgG1 Sheep IgG Sheep IgG Goat IgG Sheep IgG Goat IgG Goat IgG Rat IgG2A Goat IgG Goat IgG Rat IgG2A Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG2B Goat IgG Goat IgG Goat IgG Goat IgG CATALOG # BBA30 AF1728 BAF1728 AF3239 AF5235 AF1146 AF1615 MAB1615 AF2094 AF2108 MAB2108 AF1669 AF4065 AF3156 AF1786 AF1177 MAB1177 AF1239 BAF1239 AF2948 AF3856 SIZE 200 g 100 g 50 g 100 g 100 g 100 g 100 g 500 g 100 g 100 g 500 g 100 g 100 g 100 g 100 g 100 g 500 g 100 g 50 g 100 g 100 g PRICE $315 $355 $380 $355 $355 $355 $355 $325 $355 $355 $325 $355 $355 $355 $355 $355 $325 $355 $380 $355 $355
Semaphorin 6B Semaphorin 6C Serpin A4/Kallistatin Serpin A6 Serpin A8/Angiotensinogen Serpin E1/PAI-1 Serpin F1/PEDF
Serpin F2/a2-Antiplasmin Serum Amyloid A1 Serum Amyloid A4
Mouse Mouse Human
Serum Amyloid A4 in Human Liver. Serum amyloid A4 (SAA4) was detected in paraffin-embedded human liver tissue sections using anti-human serum amyloid A4 affinity-purified polyclonal antibody (Catalog # AF3856) and Antigen Retrieval Reagent - Basic (Catalog # CTS013). Tissues were stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
SF-1/NR5A1 sFRP-3
Human Mouse
None None Biotin None Biotin None None None
N1665 Polyclonal Polyclonal Polyclonal Polyclonal 257812 255402 427043
Mouse IgG1 Goat IgG Goat IgG Goat IgG Goat IgG Rat IgG2A Rat IgG2A Mouse IgG2B
PP-N1665-00 AF592 BAF592 AF2656 BAF2656 MAB2317 MAB1878 MAB18781
100 g 100 g 50 g 100 g 50 g 100 g 100 g 100 g
$380 $355 $380 $355 $380 $240 $240 $240
SHBG SHIP aa 874-941 SHP-1 aa 205-595
Human Human/Mouse/Rat Human Human/Mouse/Rat
SHP-1 in Human Thymus. Src-Homology 2 domain Phosphatase-1 (SHP-1) was detected in a paraffinembedded human thymus section using antihuman/mouse/rat SHP-1 monoclonal antibody (Catalog # MAB18781). The tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).
Phospho-SHP-2 (Y542) Siglec-2/CD22 Siglec-5
Human/Mouse Human Mouse Human
None None None None
Polyclonal 219934 Polyclonal 194117
Rabbit IgG Mouse IgG1 Goat IgG Mouse IgG2B
AF3790 MAB1968 AF2296 MAB1072
100 g 100 g 100 g 500 g
$285 $240 $355 $325
94

MOLECULE Siglec-5/14 Siglec-7 SPECIES Human Human LABEL None None None None Biotin None None CLONE Polyclonal Polyclonal 194212 Polyclonal Polyclonal Polyclonal Polyclonal HOST/TYPE Goat IgG Goat IgG Mouse IgG2B Goat IgG Goat IgG Goat IgG Goat IgG CATALOG # AF1072 AF1138 MAB1138 AF1139 BAF1139 AF4025 AF5638 SIZE 100 g 100 g 500 g 100 g 50 g 100 g 100 g PRICE $355 $355 $325 $355 $380 $355 $355
Siglec-9 SKI SLAIN1 aa 262-483
SLAIN1 in Mouse D3 Cells. SLAIN1 was detected in the D3 mouse embryonic stem cell line using antihuman/mouse SLAIN1 affinity-purified polyclonal antibody (Catalog # AF5638). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).
Sleeping Beauty Transposase SLITRK1
Multi-species Human
None None None None
Polyclonal 324622 327829 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 376520 378611
Goat IgG Mouse IgG1 Mouse IgG2B Total Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG2B Rat IgG1
AF2798 MAB2798 MAB30091 AB-260-NA AF1274 BAF1274 AF7891 AF2039 MAB4037 MAB4038
100 g 100 g 100 g 1 mg 100 g 50 g 100 g 100 g 100 g 100 g
$355 $240 $240 $355 $355 $380 $355 $355 $240 $240
SLPI
Human
None Biotin
SMAC/Diablo Smad1 aa 2-454 Smad2 aa 20-108 Smad3 aa 2-230
None None None None
Smad3 in Human Pancreatic Cancer. Smad3 was detected in paraffin-embedded human pancreatic cancer sections using anti-human Smad3 monoclonal antibody (Catalog # MAB4038). Tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013) and stained using the anti-rat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown). Tissue was counterstained with hematoxylin (blue).
Smad4 aa 139-332 Smad4 aa 139-332 Smad5 aa 155-258
Human Human Human
None None None
Goat IgG Mouse IgG2B Goat IgG
AF2097 MAB2097 AF2250
100 g 100 g 100 g
$355 $240 $355
Smad5 in Human Colon. Smad5 was detected in paraffin-embedded human colon tissue sections using anti-human Smad5 affinity-purified polyclonal antibody (Catalog # AF2250). Tissues were stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
95

MOLECULE Smad7 aa 320-398 SPECIES Human/Mouse/Rat LABEL None CLONE 293039 HOST/TYPE Mouse IgG2B CATALOG # MAB2029 SIZE 100 g PRICE $240
Smad7 in Human Liver Cancer. Smad7 was detected in paraffin-embedded human liver cancer sections using anti-human/mouse/rat Smad7 monoclonal antibody (Catalog # MAB2029). Tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).
Smad8 aa 158-223 SMAGP
Human Human Rat
None None None
Goat IgG Goat IgG Goat IgG
AF2309 AF3959 AF3967
100 g 100 g 100 g
$355 $355 $355
SMAGP in Human Colon. Small Transmembrane and Glycosylated Protein (SMAGP) was detected in paraffin-embedded human colon sections using antihuman SMAGP affinity-purified polyclonal antibody (Catalog # AF3959). Tissue was stained using the antigoat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
a-Smooth Muscle Actin N-terminal Synthetic Decapeptide Snail SNF1LK2 SOCS-3 aa 1-225 SOCS-5 SOD1/Cu-Zn SOD SOD2/Mn-SOD aa 25-222 Somatostatin R2/SSTR2 Sonic Hedgehog C-Terminus Sonic Hedgehog N-Terminus SorCS1 aa 111-1099 SorCS2 SorCS3 SorCS3 aa 34-1122 SorLA Sortilin
Human Human Human Human/Mouse Human Human Human/Mouse/Rat Human Mouse Mouse Human Mouse Mouse Mouse Human Human Mouse Mouse Human Human Human/Mouse
None None None None None None None None None None None None None None None None None None None Biotin None None Biotin None
1A4 Polyclonal Polyclonal 516919 Polyclonal 348808 349810 402038 Polyclonal Polyclonal 171018 Polyclonal Polyclonal Polyclonal 339615 525122 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 245610
Mouse IgG2A Goat IgG Goat IgG Mouse IgG2B Goat IgG Mouse IgG2A Mouse IgG1 Mouse IgG2A Goat IgG Goat IgG Rat IgG2A Goat IgG Sheep IgG Goat IgG Rat IgG2A Mouse IgG1 Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG2A
MAB1420 AF3639 AF5737 MAB5696 AF4796 MAB3418 MAB3419 MAB4224 AF445 AF464 MAB4641 AF3457 AF4237 AF3067 MAB30671 MAB5699 AF3154 AF2934 AF1589 BAF1589 AF3369 AF2018 BAF2018 MAB2018
100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 500 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 50 g 100 g 100 g 50 g 100 g
$240 $355 $355 $240 $355 $240 $240 $240 $355 $355 $325 $355 $355 $355 $240 $240 $355 $355 $355 $380 $355 $355 $380 $240
SOST/Sclerostin SOX1
SOX2
96

MOLECULE SPECIES LABEL NL493 SOX2 NorthernLights-conjugated Human/Mouse NL557 NL637
SOX2 in Human BG01V Cells. SOX2 was detected in the human BG01V embryonic stem cell line using anti-human/mouse SOX2 NorthernLights-637-conjugated monoclonal antibody (Catalog # NL2018V; red). Cells were counterstained with DAPI (blue). The human BG01V embryonic stem cell line was grown on Irradiated Mouse Embryonic Fibroblasts (iMEF; Catalog # PSC001).
CLONE 245610 245610 245610
HOST/TYPE Mouse IgG2A Mouse IgG2A Mouse IgG2A
CATALOG # NL2018G NL2018R NL2018V
SIZE 500 L 500 L 500 L
PRICE $345 $345 $345
SOX3 SOX7 SOX9
Human Human Human
None None None None Biotin
Polyclonal 287403 Polyclonal Polyclonal Polyclonal
Goat IgG Mouse IgG2A Goat IgG Goat IgG Goat IgG
AF2569 MAB2569 AF2766 AF3075 BAF3075
100 g 100 g 100 g 100 g 50 g
$355 $240 $355 $355 $380
SOX9 in Human HEK293 Cells. SOX9 was detected in human HEK293 embryonic kidney cell line using anti-human SOX9 affinity-purified polyclonal antibody (Catalog # AF3075). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).
SOX9 NorthernLights-conjugated SOX10 SOX10 NorthernLights-conjugated SOX15
Human Human Human/Rat Human/Rat Human
NL557 None None NL557 None None
Polyclonal Polyclonal 20B7 20B7 Polyclonal Polyclonal Polyclonal 245013 Polyclonal
Goat IgG Goat IgG Mouse IgG1 Mouse IgG1 Sheep IgG Goat IgG Goat IgG Mouse IgG3 Goat IgG
NL3075R AF2864 MAB2864 NL2864R AF4070 AF1924 BAF1924 MAB1924 NL1924R
500 L 100 g 100 g 500 L 100 g 100 g 50 g 100 g 500 L
$350 $355 $240 $345 $355 $355 $380 $240 $350
SOX17
Human
Biotin None
SOX17 NorthernLights-conjugated, aa 177-144
Human
NL557
SOX17 in Mouse Melanoma Cells. SOX17 was detected in the B16 mouse melanoma cell line using anti-human SOX17 affinity-purified polyclonal antibody (Catalog # AF1924). The cells were stained with NorthernLights-557 anti goat secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).
SOX21
None None None None None None Biotin None
Polyclonal Polyclonal 122511 124413 Polyclonal Polyclonal Polyclonal Polyclonal
Goat IgG Goat IgG Mouse IgG1 Rat IgG2B Goat IgG Goat IgG Goat IgG Rabbit IgG
AF3538 AF941 MAB941 MAB942 AF2495 AF1928 BAF1928 AF2685
100 g 100 g 500 g 500 g 100 g 100 g 50 g 100 g
$355 $355 $325 $325 $355 $355 $380 $285
SPARC/Osteonectin
Spinesin
Mouse Human
Phospho-Src (Y419)
97

MOLECULE Src Src aa 1-79 SPECIES Human Human/Mouse/Rat LABEL None None CLONE 327554 Polyclonal HOST/TYPE Mouse IgG2A Goat IgG CATALOG # MAB3807 AF3389 SIZE 100 g 100 g PRICE $240 $355
Src in Human Colon. Src was detected in paraffinembedded normal human colon sections using antihuman/rat/mouse Src affinity-purified polyclonal antibody (Catalog # AF3389). Tissue was stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
SSEA-1 SSEA-1 NorthernLights-conjugated SSEA-3 SSEA-4 STAT1 STAT2 Phospho-STAT3 (Y705)
Human/Mouse Human/Mouse Human/Mouse Human/Mouse Human Human Human
None NL557 None Biotin None None None None
MC-480 MC-480 MC-631 MC-813-70 MC-813-70 246523 545103 Polyclonal
Mouse IgM Mouse IgM Rat IgM Mouse IgG3 Mouse IgG3 Rat IgG2B Mouse IgG2B Rabbit IgG
MAB2155 NL2155R MAB1434 BAM1435 MAB1435 MAB1490 MAB16661 AF4607
100 g 500 L 100 g 100 g 100 g 100 g 100 g 100 g
$240 $345 $230 $295 $230 $240 $240 $285
Phospho-STAT3 in Human Daudi Cells. PhosphoSignal Transducer and Activator of Transcription 3 (STAT3) was detected in human INF-a-treated Daudi human cells (Y705) treated with IFN-a using antihuman phospho-STAT3 affinity-purified polyclonal antibody (Catalog # AF4607). Cells were stained using NorthernLights-557 donkey anti-rabbit IgG secondary antibody (Catalog # NL004; red) and counterstained with DAPI (blue).
STAT3 aa 1-175 STAT3 aa 688-770 STAT3 NorthernLights-conjugated Phospho-STAT5a/b (Y699)
Human Human/Mouse/Rat Human/Mouse/Rat Human
None None None NL557 None
Polyclonal 232209 Polyclonal 232209 Polyclonal
Sheep IgG Mouse IgG2B Goat IgG Mouse IgG2B Rabbit IgG
AF17991 MAB1799 AF1799 NL1799R AF4190
100 g 100 g 100 g 500 L 100 g
$355 $240 $355 $345 $285
Phospho-STAT5 (Y699) in IFN-a-treated Daudi cells. Phospho-Signal Transducer and Activator of Transcription 5 (Y699) was detected in IFN-a treated Daudi BurKitts lymphoma human cells using antihuman/mouse phosphor-STAT5 (Y699) polyclonal antibody (Catalog # AF4190). The cells were stained using NorthernLights-557 anti-rabbit secondary antibody (Catalog # NL004; red) and counterstained with DAPI (blue).
STAT5a aa 774-794 STAT6 aa 342-640
Human Human/Mouse Human
None None None
251610 251619 253906
Mouse IgG1 Mouse IgG3 Mouse IgG2A
MAB21741 MAB2174 MAB2167
100 g 100 g 100 g
$240 $240 $240
STAT6 in Human Peripheral Blood Mononuclear Cells (PBMCs). Signal Transducer and Activator of Transcription 6 (STAT6) was detected in PHA-stimulated human PBMCs using anti-human/mouse STAT6 monoclonal antibody (Catalog # MAB2167; red). Cells were counterstained (green).
98

MOLECULE SPECIES LABEL None Stella/Dppa3 Mouse Biotin None STRA6 STRO-1 Substance P SUMO3 Survivin SUZ12 Synapsin I Pan Specific Synaptophysin Synaptotagmin Human Human Human/Mouse/Rat Human Human Human Human/Mouse/Rat/Bovine Human Rat None None None None None None None None None None CLONE Polyclonal Polyclonal 283910 496613 STRO-1 266815 401513 Polyclonal 448909 Polyclonal Polyclonal ASV48 ASV30 HOST/TYPE Goat IgG Goat IgG Rat IgG2A Mouse IgG1 Mouse IgM Mouse IgG1 Rat IgG2A Rabbit IgG Mouse IgG1 Rabbit IgG Goat IgG Mouse IgG2B Mouse IgG2A CATALOG # AF2566 BAF2566 MAB2566 MAB5904 MAB1038 MAB4375 MAB2959 AF886 MAB4184 PPS035 AF5555 MAB4364 MAB43641 SIZE 100 g 50 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 10 g 100 g 100 g 100 g PRICE $355 $380 $240 $240 $295 $240 $240 $285 $240 $355 $355 $240 $240
Synaptotagmin I in Rat Spinal Cord. Synaptotagmin I was detected in a cryostat section of rat spinal cord using anti-rat Synaptotagmin I monoclonal antibody (Catalog # MAB43641; red). The tissue was counterstained green.
Syndecan-1/CD138
Human Human
None Biotin None None None None None None None None None None None None None None None None None Biotin None
Polyclonal Polyclonal 359103 Polyclonal 300506 Polyclonal 305507 374412 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 516505 514304 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 165609 Polyclonal Polyclonal Polyclonal 525803 393720 Polyclonal
Goat IgG Goat IgG Rat IgG1 Goat IgG Rat IgG1 Goat IgG Rat IgG2A Rat IgG2A Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Sheep IgG Mouse IgG2B Mouse IgG1 Goat IgG Goat IgG Chicken IgY Chicken IgY Goat IgG Goat IgG Mouse IgG1 Goat IgG Goat IgG Goat IgG Mouse IgG1 Rat IgG2A Goat IgG
AF2780 BAF2780 MAB2780 AF3190 MAB2966 AF2965 MAB29651 MAB35391 AF2734 AF3539 AF2918 AF5675 AF1338 AF5528 MAB5528 MAB5745 AF3578 AF2129 AF930 BAF930 AF174 BAF174 MAB174 AF1041 BAF1041 AF3494 MAB5385 MAB50401 AF4509
$355 $380 $240 $355 $240 $355 $240 $240 $355 $355 $355 $355 $355 $355 $240 $240 $355 $355 $355 $380 $355 $380 $325 $355 $380 $355 $240 $240 $355
Syndecan-1/CD138 Mouse
Syndecan-2
Human Human Mouse Human Human Human/Mouse/Rat Human Human Human Human/Mouse Human Human
Syndecan-3 Syndecan-3 Isoform 1 Syndecan-4 Syntaxin-BP1 Synuclein-a Synuclein-b Synuclein-g TAB1 aa 7-159 TACE/ADAM17 Cytosolic Domain TACE/ADAM17 Ectodomain
Human TACI/TNFRSF13B Mouse Tau T-bet/TBX21 TBX2 TBX3 aa 321-483 Multi-species Human Human Human
Biotin None None Biotin None None None None
99

MOLECULE TBX6 SPECIES Human LABEL None CLONE Polyclonal HOST/TYPE Goat IgG CATALOG # AF4744 SIZE 100 g PRICE $355
TBX6 in Mouse Embryonic Mesoderm. T-box Protein 6 (TBX6) was detected in E9.5 mouse embryonic tailbud presomitic mesoderm sections using antihuman TBX6 affinity-purified polyclonal antibody (Catalog # AF4744). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).
TC21/R-Ras2 TCCR/WSX-1 TCF-2/HNF-1b TCF-7/TCF-1 TCL1A TEM8 Isoform 1
Human/Rat Human Human Human Human Human Human Human/Mouse
Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 391819 578 Polyclonal 619
Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Rat IgG2A Rat IgG2A Goat IgG Mouse IgG1
AF3605 AF1479 AF3330 AF5596 AF4847 AF3886 AF3358 MAB3358 MAB2138 AF3865 MAB1624
100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g 100 g
$355 $355 $355 $355 $355 $355 $355 $240 $240 $355 $240
Tenascin C
Tenascin R
Human Mouse/Rat
Tenascin R in Human Brainstem. Tenascin R was detected in paraffin-embedded human brainstem (medulla) tissue sections using anti-human Tenascin R affinity-purified polyclonal antibody (Catalog # AF3865). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
TER-119 TFF3
Mouse Human
Biotin None None
TER-119 TER-119 415909
Rat IgG2B Rat IgG2B Mouse IgG1
BAM1125 MAB1125 MAB4407
250 g 500 g 100 g
$380 $325 $240
TFF3 in Human Colon. Trefoil factor 3 (TFF3) was detected in a paraffin-embedded human colon section using anti-human TFF3 monoclonal antibody (Catalog # MAB4407). The tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).
TFPI-2 TGF-a
Human Human
None None None None None None None None None None
243220 Polyclonal Polyclonal Polyclonal Polyclonal 9016 1D11 8607 Polyclonal Polyclonal 20724 44922
Mouse IgG2A Total Goat IgG Goat IgG Total Goat IgG Goat IgG Mouse IgG1 Mouse IgG1 Mouse IgG2B Goat IgG Goat IgG Mouse IgG1 Mouse IgG1
MAB2545 AB-239-NA AF-239-NA AB-246-NA AF-246-NA MAB240 MAB1835 MAB612 AF-302-NA AF-243-NA MAB243 MAB643
500 g 1 mg 100 g 1 mg 100 g 500 g 500 g 500 g 100 g 100 g 500 g 500 g
$325 $355 $355 $355 $355 $325 $325 $325 $355 $355 $325 $325
LAP TGF-b1 TGF-b1 TGF-b1, 2, 3 TGF-b2 TGF-b2/1.2
Human Multi-species Multi-species Multi-species Multi-species
TGF-b3
Multi-species
None None
100

MOLECULE TGF-b RI/ALK-5 TGF-b RII Thioredoxin-1 Thioredoxin Reductase 2/TRXR2 aa 268-393 Thimet Oligopeptidase/THOP1 SPECIES Human Human Human Human/Mouse Human Human Thrombomodulin/THBD/CD141 Mouse Human Human Human/Mouse Human Zebrafish LABEL None None None None None None None None None None None None None None CLONE Polyclonal Polyclonal Polyclonal 575322 Polyclonal 501733 Polyclonal 461714 301221 Polyclonal 88016 Polyclonal Polyclonal Polyclonal HOST/TYPE Goat IgG Goat IgG Goat IgG Mouse IgG1 Sheep IgG Mouse IgG1 Goat IgG Rat IgG2B Mouse IgG2B Goat IgG Mouse IgG1 Rabbit IgG Goat IgG Goat IgG CATALOG # AF3025 AF-241-NA AF1970 MAB5815 AF3439 MAB3947 AF3894 MAB3894 MAB3074 AF619 MAB619 AF3909 AF313 AF928 SIZE 100 g 250 g 100 g 100 g 100 g 100 g 100 g 100 g 500 g 100 g 500 g 100 g 100 g 100 g PRICE $355 $355 $355 $240 $355 $240 $355 $240 $325 $355 $325 $285 $355 $355
Thrombospondin-1 Tie-1 Phospho-Tie-2 (Y1100) Tie-2
Tie-2 in Developing Blood Vessels of Zebrafish. Tie-2 was detected in the blood vessels of an embryonic zebrafish using anti-zebrafish Tie-2 affinity-purified polyclonal antibody (Catalog # AF928). The tissue was stained using NorthernLights-557 donkey antigoat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).
TIM-1/KIM-1/HAVCR
Human Rat
None None
219211 Polyclonal
MAB1750 AF3689
500 g 100 g
$325 $355
TIM-1 in Rat Kidney. T cell Immunoglobulin and Mucin domain-containing (TIM-1), also known as Kidney Injury Molecule 1 (KIM-1), was detected in frozen sections of rat kidney using anti-rat TIM-1 affinity-purified polyclonal antibody (Catalog # AF3689). Tissue was stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Human TIMP-1 Mouse Rat TIMP-2 Human
63515 Polyclonal Polyclonal Polyclonal 89025
Mouse IgG2B Goat IgG Goat IgG Goat IgG Mouse IgG1
MAB970 AF980 AF580 AF971 MAB971
500 g 100 g 100 g 100 g 500 g
$325 $355 $355 $355 $325
TIMP-2 in Human Ovarian Cancer. Tissue Inhibitor of Metalloproteinase 2 (TIMP-2) was detected in a paraffin-embedded tissue section of human ovarian cancer using anti-human TIMP-2 affinity-purified polyclonal antibody (Catalog # AF971). Tissues were stained with the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown), and counterstained with hematoxylin (blue).
TIMP-3 TIMP-4 TLR4 TMEFF1/Tomoregulin-1
None None None None Biotin
183551 153934 Polyclonal Polyclonal Polyclonal
Mouse IgG1 Mouse IgG1 Goat IgG Goat IgG Goat IgG
MAB973 MAB974 AF1478 AF1995 BAF1995
500 g 500 g 100 g 100 g 50 g
$325 $325 $355 $355 $380
101

MOLECULE SPECIES Bovine LABEL None Biotin None Canine Biotin None Equine TNF-a Human None None Biotin None Mouse Porcine Rat TNF-b/TNFSF1 Human Mouse Human Mouse None None None None None Biotin None None CLONE Polyclonal Polyclonal Polyclonal Polyclonal 236812 Polyclonal Polyclonal Polyclonal 28401 Polyclonal Polyclonal 103302 Polyclonal 5802 Polyclonal Polyclonal Polyclonal HOST/TYPE Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG1 Goat IgG Goat IgG Goat IgG Mouse IgG1 Goat IgG Goat IgG Mouse IgG1 Goat IgG Mouse IgG1 Goat IgG Goat IgG Goat IgG CATALOG # AF2279 BAF2279 AF1507 BAF1507 MAB1507 AF1814 AF-210-NA BAF210 MAB610 AF-410-NA AF690 MAB6903 AF-510-NA MAB211 BAF749 AF225 AF-425-PB SIZE 100 g 50 g 100 g 50 g 500 g 100 g 100 g 50 g 500 g 100 g 100 g 100 g 100 g 500 g 50 g 100 g 100 g PRICE $355 $380 $355 $380 $325 $355 $355 $380 $325 $355 $355 $240 $355 $325 $380 $355 $355
TNF RI/TNFRSF1A
TNF RI in Human Peripheral Blood Lymphocytes. Tumor Necrosis Factor Receptor I (TNF RI) was detected in human peripheral blood lymphocytes using anti-human TNF RI affinity-purified polyclonal antibody (Catalog # AF225; red) and cells were counterstained green.
TNF RII/TNFRSF1B
Human Mouse
None None None None
Polyclonal 22221 Polyclonal 303728
Total Goat IgG Mouse IgG2A Goat IgG Rat IgG2A
AB-226-PB MAB226 AF-426-PB MAB1537
1 mg 500 g 100 g 100 g
$355 $325 $355 $240
TOR
Human/Mouse
TOR in HEK293 cells. Target of rapamycin (TOR) expression was detected in HEK293 human embryonic kidney cells using anti-human TOR monoclonal antibody (Catalog # MAB1537). Cells were stained using NorthernLights-557 goat anti-rat IgG secondary antibody (Catalog # NL013; red) and counterstained with DAPI (blue).
TRa/NR1A1 TR4/NR2C2 TRA-1-85 TRADD
None None Biotin None None None
H2804 H0107B TRA-1-85 TRA-1-85 Polyclonal Polyclonal 75411 75402 Polyclonal Polyclonal Polyclonal 118929
Mouse IgG2A Mouse IgG2A Mouse IgG1 Mouse IgG1 Goat IgG Goat IgG Mouse IgG1 Mouse IgG1 Goat IgG Goat IgG Goat IgG Rat IgG2A
PP-H2804-00 PP-H0107B-00 BAM3195 MAB3195 AF2658 AF375 MAB375 MAB687 AF1121 BAF1121 AF347 MAB1540
100 g 100 g 100 g 100 g 100 g 100 g 500 g 500 g 100 g 50 g 100 g 500 g
$380 $380 $240 $240 $355 $355 $325 $325 $355 $380 $355 $325
Human TRAIL/TNFSF10 Mouse TRAIL R1/TNFRSF10A TRAIL R2/TNFRSF10B Human Mouse
102

MOLECULE TRAIL R3/TNFRSF10C SPECIES Human LABEL None None CLONE Polyclonal 90905 HOST/TYPE Goat IgG Mouse IgG1 CATALOG # AF630 MAB6301 SIZE 100 g 500 g PRICE $355 $325
TRAIL Receptor 3 in Human Spleen. Tumor Necrosis Factor-related Apoptosis-inducing Ligand Receptor 3 (TRAIL R3) was detected in paraffin-embedded human spleen tissue sections using anti-human TRAIL Receptor 3 monoclonal antibody (Catalog # MAB630). Tissues were subjected to antigen retrieval using basic reagent (Catalog # CTS013) and stained using anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown). Tissues were counterstained with hematoxylin (blue).
TRAIL R4/TNFRSF10D TRANCE/TNFSF11/RANK L TRANCE/TNFSF11/RANK L TfR (Transferrin R)
Polyclonal 70525 Polyclonal Polyclonal Polyclonal 174031 174031 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 75133 Polyclonal Polyclonal 225105 Polyclonal 75219 Polyclonal Polyclonal
Goat IgG Mouse IgG2B Goat IgG Goat IgG Goat IgG Rat IgG2A Rat IgG2A Goat IgG Goat IgG Sheep IgG Sheep IgG Goat IgG Goat IgG Goat IgG Mouse IgG2B Goat IgG Goat IgG Rat IgG2B Goat IgG Mouse IgG2B Goat IgG Goat IgG
AF633 MAB626 AF462 AF2474 AF1187 BAM1187 MAB1187 AF1828 BAF1828 AF1729 BAF1729 AF175 AF1056 BAF1056 MAB397 AF1494 BAF1494 MAB1494 AF373 MAB373 AF1404 BAF1404
100 g 500 g 100 g 100 g 100 g 100 g 500 g 100 g 50 g 100 g 50 g 100 g 100 g 50 g 500 g 100 g 50 g 500 g 100 g 500 g 100 g 50 g
$355 $325 $355 $355 $355 $380 $325 $355 $380 $355 $380 $355 $355 $380 $325 $355 $380 $325 $355 $325 $355 $380
TREM-1
Mouse
Biotin None
Human TREM-2 Mouse Human TrkA Rat Human TrkB
None Biotin None Biotin None None Biotin None None
Mouse
Biotin None
Human TrkC Mouse
TrkC in Rat Spinal Cord. TrkC was detected in a cryostat tissue section of rat spinal cord using antihuman TrkC monoclonal antibody (Catalog # MAB373; red).
103

MOLECULE SPECIES Human TROP-2 Mouse Troponin T (Cardiac) TROY/TNFRSF19 Trypsin Pan Specific (PRSS1/2/3) Trypsin 1/PRSS1 Trypsin 3/PRSS3 Human Mouse Human Human Mouse LABEL None Biotin None Biotin None None Biotin None None None None CLONE Polyclonal Polyclonal Polyclonal Polyclonal 200805 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 419911 HOST/TYPE Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG2A Goat IgG Goat IgG Sheep IgG Sheep IgG Goat IgG Rat IgG1 CATALOG # AF650 BAF650 AF1122 BAF1122 MAB1874 AF723 BAF723 AF3586 AF3848 AF3565 MAB3565 SIZE 100 g 50 g 100 g 50 g 100 g 100 g 50 g 100 g 100 g 100 g 100 g PRICE $355 $380 $355 $380 $240 $355 $380 $355 $355 $355 $240
Trypsin 3 in Mouse Pancreas. Trypsin 3 was detected in a frozen section of mouse pancreas using antimouse Trypsin-3 monoclonal antibody (Catalog # MAB3565). Tissues were stained using anti-rat HRPDAB reagents (brown) and counterstained with hematoxylin (blue).
Trypsin-3 in Mouse Kidney. Trypsin-3 was detected in frozen mouse kidney sections using anti-mouse Trypsin-3 monoclonal antibody (Catalog # MAB3565). Tissue was stained using the anti-rat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS017; brown) and counterstained with hematoxylin (blue).
Tryptophan Hydroxylase Pan Specific Tryptophan Hydroxylase 1 TSG TSG
Multi-species Human Mouse Mouse Human
None None None Biotin None None None Biotin None None None Biotin None
Polyclonal Polyclonal Polyclonal 120024 120024 Polyclonal Polyclonal Polyclonal 512913 Polyclonal 458811 TuJ-1 TuJ-1
Sheep IgG Goat IgG Goat IgG Rat IgG2A Rat IgG2A Goat IgG Goat IgG Goat IgG Mouse IgG1 Goat IgG Rat IgG2B Mouse IgG2A Mouse IgG2A
PPS038 AF5276 AF756 BAM756 MAB756 AF2104 AF2326 BAF2326 MAB5794 AF981 MAB4734 BAM1195 MAB1195
100 L 100 g 100 g 250 g 500 g 100 g 100 g 50 g 100 g 100 g 100 g 100 g 100 g
$355 $355 $355 $380 $325 $355 $355 $380 $240 $355 $240 $380 $240
TSG-6
Mouse Human Human Human Multi-species
TSH b TSLP R TSPAN8 Neuron-specific b-III Tubulin
b-III Tubulin and Nestin in Rat Cortical Stem Cells. b-III Tubulin and Nestin were detected in rat cortical stem cells (Catalog # NSC001) using anti-neuron specific b-III Tubulin monoclonal (clone TuJ-1) antibody (Catalog # MAB1195; red) and anti rat Nestin affinitypurified polyclonal antibody (Catalog # AF2736; green). Cells were stained with NorthernLights-557 donkey anti-mouse IgG (Catalog # NL007) and NorthernLights-493 donkey anti-goat IgG (Catalog # NL003) secondary antibodies. Tissue was counterstained with DAPI (blue).
b-III Tubulin and GFAP in Adult Mouse Brain. Neuronal b-III Tubulin and astrocytic GFAP were detected in adult mouse brain sections using anti-b-III Tubulin monoclonal antibody (Catalog # MAB1195; Tuj1) and anti-human GFAP affinity-purified polyclonal antibody (Catalog # AF2594). The tissue was stained using NorthernLights-493 donkey anti-mouse (Catalog # NL009; green) and NorthernLights-557 donkey anti-sheep IgG (Catalog # NL010; red) secondary antibodies, respectively. The nuclei were stained using DAPI (blue)
Neuron-specific b-III Tubulin NorthernLights-conjugated TWEAK/TNFSF12 TWEAK R/TNFRSF12
Multi-species Human Human Mouse Multi-species
NL557 NL637 Biotin None None None None
TuJ-1 TuJ-1 Polyclonal Polyclonal Polyclonal 179003 216954
Mouse IgG2A Mouse IgG2A Goat IgG Goat IgG Goat IgG Mouse IgG1 Rat IgG2A
NL1195R NL1195V BAF1090 AF1199 AF1610 MAB1676 MAB16761
500 L 500 L 50 g 100 g 100 g 500 g 500 g
$345 $345 $380 $355 $355 $325 $325
Phospho-Phospho-Tyrosine
104

MOLECULE Phospho-Tyrosine Hydroxylase (S19) Phospho-Tyrosine Hydroxylase (S31) Phospho-Tyrosine Hydroxylase (S40) Tyrosine Hydroxylase Ubiquitin/Ubiquitin+1 Ubiquitin+1 ULBP-2 UNC5H1 UNC5H2 SPECIES Rat Mouse/Rat/Primate Rat Multi-species Human Human Human Rat Rat LABEL None None None None None None None None None None Biotin None None None None None CLONE Polyclonal Polyclonal Polyclonal TH-2 6D7 83406 82001 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 200414 Polyclonal Polyclonal Polyclonal HOST/TYPE Rabbit IgG Rabbit IgG Rabbit IgG Mouse IgG1 Mouse IgG1 Mouse IgG2B Mouse IgG2A Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG2B Goat IgG Goat IgG Sheep IgG CATALOG # PPS087 PPS088 PPS041 MAB1423 PPS040 MAB701 MAB703 AF1298 AF1405 AF1006 BAF1006 AF1005 MAB1005 AF1310 AF534 AF5144 SIZE 100 L 100 L 100 L 100 g 100 L 100 g 100 g 100 g 100 g 100 g 50 g 100 g 500 g 100 g 100 g 100 g PRICE $355 $355 $355 $240 $355 $240 $240 $355 $355 $355 $380 $355 $325 $355 $355 $355
UNC5H3 u-Plasminogen Activator (uPA)/Urokinase uPAR Uromodulin
Uromodulin in Human Kidney. Uromodulin was detected in paraffin-embedded human kidney sections using anti-human Uromodulin affinity-purified polyclonal antibody (Catalog # AF5144). Tissue was stained using anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).
Uteroglobin/SCGB1A1 UTF1 VAMP-1
None None None
Rat IgG1 Goat IgG Goat IgG
MAB4218 AF3958 AF4828
100 g 100 g 100 g
$240 $355 $355
VAMP-1 in Mouse Brain. Vesicle-associated membrane protein 1 (VAMP-1) was detected in frozen sections of mouse brain (spinal cord) using anti-human/ mouse VAMP-1 affinity-purified polyclonal antibody (Catalog # AF4828). The tissue was stained using antigoat IgG NorthernLights-557-conjugated secondary antibody (Catalog # NL001; red) and counterstained green.
Vang-like Protein 1/VANGL1 Vanilloid R1/TRPV1
Human Rat
None None
497707 Polyclonal
MAB5476 AF3066
100 g 100 g
$240 $355
Vanilloid R1 in Rat Dorsal Root Ganglion. Vanilloid R1 was detected in a frozen section of rat dorsal root ganglion using anti-rat Vanilloid R1 affinity-purified polyclonal antibody (Catalog # AF3066; red) and counterstained green.
Vanilloid R-like 3/TRPV3 VASA
Human Human
None None
Sheep IgG Goat IgG
AF4167 AF2030
100 g 100 g
$355 $355
105

MOLECULE SPECIES LABEL None VCAM-1/CD106 Human None None Mouse VDR/NR1I1 VEGF Human Canine Human Canine VEGF 164 Mouse Rat None None None None None Biotin None None CLONE Polyclonal BBIG-V1 BBIG-V1 Polyclonal H4537 247109 26503 Polyclonal Polyclonal Polyclonal Polyclonal HOST/TYPE Goat Serum Mouse IgG1 Mouse IgG1 Goat IgG Mouse IgG2A Mouse IgG1 Mouse IgG2B Goat IgG Goat IgG Goat IgG Goat IgG CATALOG # BBA19 BBA5 BBA6 AF643 PP-H4537-00 MAB1603 MAB293 AF1603 BAF1603 AF-493-NA AF564 SIZE 500 L 100 g 1 mg 100 g 100 g 500 g 500 g 100 g 50 g 100 g 100 g PRICE $195 $230 $840 $355 $380 $325 $325 $355 $380 $355 $355
VEGF in Mouse Kidney. Vascular Endothelial Growth Factor (VEGF) was detected in a cryostat section of mouse kidney glomerulus using anti-mouse VEGF affinity-purified polyclonal antibody (Catalog # AF493-NA; red) and counterstained green.
VEGF in Rat Kidney. Vascular Endothelial Growth Factor (VEGF) was detected in a cryostat section of rat kidney glomerulus using anti-rat VEGF 164 affinitypurified polyclonal antibody (Catalog # AF564). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
VEGF 165 VEGF 165b VEGF-B 167/186 VEGF-B 186 VEGF-C
Human Human Human/Mouse Mouse Human Human Mouse Human Mouse Human
None None None None None None None None None None Biotin None None None None None None None None None None None None
Polyclonal Polyclonal 56-1 58013 Polyclonal Polyclonal Polyclonal 78923 Polyclonal Polyclonal Polyclonal 141515 Polyclonal 89115 Polyclonal 522302 Polyclonal 54703 Polyclonal 368011 237018 Polyclonal 280618
Total Goat IgG Goat IgG Mouse IgG1 Mouse IgG1 Goat IgG Goat IgG Goat IgG Mouse IgG1 Goat IgG Goat IgG Goat IgG Rat IgG2A Goat IgG Mouse IgG1 Goat IgG Rat IgG1 Goat IgG Mouse IgG1 Goat IgG Rat IgG2A Mouse IgG1 Goat IgG Rat IgG2A
AB-293-NA AF-293-NA MAB3045 MAB751 AF767 AF752 AF286 MAB286 AF469 AF321 BAF321 MAB471 AF357 MAB3571 AF644 MAB4432 AF349 MAB3491 AF3054 MAB30481 MAB16541 AF2105 MAB2105
1 mg 100 g 500 g 500 g 100 g 100 g 100 g 500 g 100 g 100 g 50 g 500 g 100 g 500 g 100 g 100 g 100 g 500 g 100 g 100 g 100 g 100 g 100 g
$355 $355 $325 $325 $355 $355 $355 $325 $355 $355 $380 $325 $355 $325 $355 $240 $355 $325 $355 $240 $240 $355 $240
VEGF-D
VEGF R1/Flt-1
VEGF R2/KDR/Flk-1 Mouse
VEGF R3/Flt-4 Versican Isoform V0 VG5Q VHR Vimentin
Human Human Mouse Human Human
Vimentin in Human Lung Carcinoma Cells. Vimentin was detected in cultured A549 human lung carcinoma cells cells using anti-human Vimentin monoclonal antibody (Catalog # MAB2105). The Cells were stained using NorthernLights-493 anti-rat secondary antibodies (Catalog # NL015; green) and the nuclei counterstained with DAPI (blue).
106
IHC/ICC SUPPLEMENTAL REAGENTS

MOLECULE VIP R2 Vitronectin VLDL R VSIG1 SPECIES Human Human Mouse Mouse Human LABEL None None None None None CLONE 476031 342603 347317 Polyclonal Polyclonal HOST/TYPE Mouse IgG1 Mouse IgG2B Rat IgG2A Goat IgG Sheep IgG CATALOG # MAB5416 MAB2349 MAB38751 AF2258 AF4818 SIZE 100 g 100 g 100 g 100 g 100 g PRICE $240 $240 $240 $355 $355
VSIG1 in Human Testis. V-set and Ig domain-containing protein 1 (VSIG1) was detected in frozen a section of human testis using anti-human VSIG1 affinity-purified polyclonal antibody (Catalog # AF4818). Cells were stained using NorthernLights-557 donkey anti-sheep IgG secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue).
WASP
Human Human/Mouse Human Human
None None None None None None Biotin None None
336925 Polyclonal 133015 133015 Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal
Mouse IgG1 Goat IgG Mouse IgG1 Mouse IgG1 Goat IgG Sheep IgG Sheep IgG Goat IgG Goat IgG
MAB3070 AF3070 MAB134-050 MAB134-500 AF1627 AF1680 BAF1680 AF1620 AF3464
100 g 100 g 50 g 500 g 100 g 100 g 50 g 100 g 100 g
$240 $355 $150 $325 $355 $355 $380 $355 $355
WIF-1
WISP-1/CCN4
Mouse Mouse Human
Wnt-1 Wnt-2
Wnt-2 in Human Stomach Cancer. Wnt-2 was detected in a paraffin-embedded human stomach cancer section using anti-human Wnt-2 affinity-purified polyclonal antibody (Catalog # AF3464). The section was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013) and stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown). The tissue was counterstained with hematoxylin (blue).
Wnt-2b Wnt-4 Wnt-5a
Mouse Mouse Mouse
None None None
Goat IgG Goat IgG Goat IgG
AF3900 AF475 AF645
100 g 100 g 100 g
$355 $355 $355
Wnt-5a in Embryonic Mouse Rib. Wnt-5a was detected in a paraffin-embedded tissue section of E15 mouse rib using anti-mouse Wnt-5a affinity-purified polyclonal antibody (Catalog # AF645). The tissue was stained using the anti-goat HRP-DAB (brown) Cell and Tissue Staining Kit (Catalog # CTS008) and counterstained with hematoxylin (blue).
Wnt-5b Wnt-6 Wnt-7a Wnt-7b Wnt-8a Wnt-8b
Mouse Human Human Human Mouse Human/Mouse
325004 Polyclonal Polyclonal Polyclonal Polyclonal 341504
Rat IgG2A Sheep IgG Goat IgG Goat IgG Goat IgG Rat IgG2A
MAB3006 AF4109 AF3008 AF3460 AF2248 MAB3367
100 g 100 g 100 g 100 g 100 g 100 g
$240 $355 $355 $355 $355 $240
107

MOLECULE Wnt-9a SPECIES Human LABEL None None CLONE Polyclonal 326707 HOST/TYPE Goat IgG Mouse IgG2A CATALOG # AF3157 MAB31571 SIZE 100 g 100 g PRICE $355 $240
Wnt-9a in Human Breast Cancer. Wnt-9a was detected in a paraffin-embedded human breast cancer section using anti-human Wnt-9a monoclonal antibody (Catalog # MAB31571). The section was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013) and stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown). The tissue was counterstained with hematoxylin (blue).
Wnt-9b Wnt-11 XCL1/Lymphotactin XCR1 XIAP
Mouse Mouse Mouse Human Human Human/Mouse/Rat Human/Mouse/Rat Human/Rat Human
None None None Biotin None None None None None None
Polyclonal Polyclonal Polyclonal Polyclonal Polyclonal 117318 Polyclonal 339827 Polyclonal Polyclonal
Goat IgG Goat IgG Goat IgG Goat IgG Goat IgG Mouse IgG1 Goat IgG Mouse IgG2B Goat IgG Goat IgG
AF3669 AF2647 AF486 BAF486 AF857 MAB8221 AF8221 MAB32051 AF3205 AF3709
100 g 100 g 100 g 50 g 100 g 100 g 100 g 100 g 100 g 100 g
$355 $355 $355 $380 $355 $240 $355 $240 $355 $355
Yes aa 1-88 ZAP70 aa 163-254
ZAP70 in Human Lymphoma. Zeta-chain (TCR) associated protein kinase 70 (ZAP70) was detected in a paraffin-embedded human lymphoma section using anti-human ZAP70 affinity-purified polyclonal antibody (Catalog # AF3709). The tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013) and stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown). The section was counterstained with hematoxylin (blue).
ZIC1
Human/Mouse
None
Polyclonal
Goat IgG
AF4978
100 g
$355
ZIC1 in Embryonic Mouse Brain. Zinc-finger protein of the cerebellum 1 (ZIC1) was detected in a frozen section of E13 mouse brain using anti-human ZIC1 affinity-purified polyclonal antibody (Catalog # AF4978). The tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
ZNF24 ZNF206
Human Human
None None
Goat IgG Goat IgG
AF3637 AF4020
100 g 100 g
$355 $355
108

Secondary & Control Antibodies for IHC/ICC SECONDARY ANTIBODIES
ANALYTE Chicken IgY LABEL None Biotin None Biotin Biotin Biotin HRP Goat IgG HRP HRP APC Fluorescein PE None Goat IgG (H+L) PerCP APC Hamster IgG PE None Biotin Human IgG Fc Human IgG1 Fc Human IgM Human IgE Human IgG (H+L) None None None None None PerCP None Mouse IgG Biotin Biotin HRP HRP Mouse IgG Fc Mouse IgG None None APC Mouse IgG F(ab)2 (H+L) Fluorescein PE None Biotin HRP Rabbit IgG APC Fluorescein PE None HRP Rat IgG None PerCP Biotin Rat IgG F(ab)2 Rat IgG F(ab)2 (H+L) APC Fluorescein PE TYPE Chicken IgY specific polyclonal goat IgG Chicken IgY specific polyclonal goat IgG Goat IgG specific polyclonal donkey IgG Goat IgG specific polyclonal donkey IgG Goat IgG specific polyclonal rabbit IgG Goat IgG specific polyclonal chicken IgY Goat IgG specific polyclonal donkey IgG Goat IgG specific polyclonal rabbit IgG Goat IgG specific polyclonal chicken IgG Goat IgG specific polyclonal donkey IgG Goat IgG specific polyclonal donkey IgG Goat IgG specific polyclonal donkey IgG Goat IgG specific polyclonal rabbit IgG Goat IgG specific polyclonal donkey IgG (H+L) chains Monoclonal mouse IgG2B, clone # MAH1.12 Monoclonal mouse IgG2B, clone # MAH1.12 Monoclonal mouse IgG2B, clone # MAH1.12 Monoclonal mouse IgG2B, clone # MAH1.12 Human IgG Fc specific polyclonal goat IgG Human IgG1 Fc specific monoclonal mouse IgG1 Human IgM specific polyclonal goat IgG Human IgE specific polyclonal goat IgG Human IgG specific polyclonal goat IgG (H+L) chains Mouse IgG specific polyclonal goat IgG F(ab')2 (H+L) Fragment Mouse IgG specific polyclonal goat IgG Mouse IgG specific polyclonal goat IgG Mouse IgG specific polyclonal donkey IgG Mouse IgG specific polyclonal goat IgG Mouse IgG specific polyclonal donkey IgG Mouse IgG Fc specific polyclonal goat IgG Mouse IgG specific polyclonal donkey IgG Mouse IgG specific polyclonal goat IgG F(ab)2 (H+L) Fragment Mouse IgG specific polyclonal goat IgG F(ab)2 (H+L) Fragment Mouse IgG specific polyclonal goat IgG F(ab)2 (H+L) Fragment Rabbit IgG specific polyclonal goat IgG Rabbit IgG specific polyclonal goat IgG Rabbit IgG specific polyclonal goat IgG Rabbit IgG specific polyclonal goat IgG Rabbit IgG specific polyclonal goat IgG Rabbit IgG specific polyclonal goat IgG Rabbit IgG specific polyclonal donkey IgG Rat specific polyclonal goat IgG Rat IgG specific polyclonal goat IgG Rat IgG specific polyclonal goat IgG F(ab')2 (H+L) Fragment Rat IgG specific polyclonal goat IgG Rat IgG specific polyclonal goat IgGF(ab)2 (H+L) Fragment Rat IgG specific polyclonal goat IgG F(ab)2 (H+L) Fragment Rat IgG specific polyclonal goat IgG F(ab)2 (H+L) Fragment APPLICATIONS Western blot Western blot Western blot Western blot Western blot Western blot Western blot Western blot Western blot Flow Cytometry Flow Cytometry Flow Cytometry Western blot Flow Cytometry Flow Cytometry Flow Cytometry Western blot Western blot Western blot Western blot Western blot Western blot Western blot Flow Cytometry Western blot Western blot Western blot Western blot Western blot Western blot Western blot Flow Cytometry Flow Cytometry Flow Cytometry Western blot Western blot Western blot Flow Cytometry Flow Cytometry Flow Cytometry Western blot Western blot Western blot Flow Cytometry Western blot Flow Cytometry Flow Cytometry Flow Cytometry CATALOG # AF010 BAF010 AF109 BAF109 BAF017 BAF019 HAF109 HAF017 HAF019 F0108 F0109 F0107 R-401-C-ABS F0124 F0121 F0120 MAB011 BAM011 G-102-C MAB110 G-105-C G-107-C G-101-C-ABS F0114 AF007 BAF007 BAF018 HAF007 HAF018 G-202-C D-201-C-ABS2 F0101B F0103B F0102B AF008 BAF008 HAF008 F0111 F0112 F0110 D-301-C-ABS2 HAF005 AF005 F0115 BAF005 F0113 F0104B F0105B SIZE 500 g 250 g 500 g 250 g 250 g 250 g 1 mL 1 mL 1 mL 100 Tests 100 Tests 100 Tests 500 g 100 Tests 100 Tests 100 Tests 500 g 250 g 100 g 500 g 100 g 100 g 500 g 100 Tests 500 g 250 g 250 g 1 mL 1 mL 100 g 500 g 100 Tests 100 Tests 100 Tests 500 g 250 g 1 mL 100 Tests 100 Tests 100 Tests 500 g 1 mL 500 g 100 Tests 250 g 100 Tests 100 Tests 100 Tests PRICE $75 $75 $75 $75 $75 $75 $95 $95 $95 $220 $140 $175 $75 $190 $220 $175 $165 $180 $355 $325 $355 $355 $75 $155 $99 $100 $75 $105 $95 $355 $75 $175 $105 $135 $75 $75 $95 $220 $140 $175 $75 $105 $100 $190 $100 $210 $120 $175
109

SECONDARY ANTIBODIES
ANALYTE LABEL Biotin Sheep IgG HRP None APC Mouse IgM Fluorescein PerCP PE TYPE Sheep IgG specific polyclonal donkey IgG Sheep IgG specific polyclonal donkey IgG Sheep IgG specific polyclonal donkey IgG Mouse IgM specific polyclonal goat IgG Mouse IgM specific polyclonal goat IgG Mouse IgM specific polyclonal goat IgG Mouse IgM specific polyclonal goat IgG APPLICATIONS Western blot Western blot Western blot Flow Cytometry Flow Cytometry Flow Cytometry Flow Cytometry CATALOG # BAF016 HAF016 D-501-C-ABS F0117 F0118 F0119 F0116 SIZE 250 g 1 mL 500 g 100 Tests 100 Tests 100 Tests 100 Tests PRICE $75 $95 $75 $220 $140 $190 $175
NorthernLights A line of fluorochrome-conjugated secondary reagents for IHC/ICC NorthernLights secondary antibodies are bright and fade-resistant, recognizing mouse, goat, sheep, rat, and rabbit IgG and chicken IgY. Like all R&DSystems antibodies, NorthernLights antibody conjugates are specific and deliver a high signal-to-noise ratio. Also available are streptavidin conjugates for labeling biotinylated antibodies.
DESCRIPTION CONJUGATE NorthernLights-557 Donkey Anti-Goat IgG, Cross-adsorbed NorthernLights-637 NorthernLights-493 NorthernLights-557 Donkey Anti-Rabbit IgG, Cross-adjusted NorthernLights-637 NorthernLights-493 NorthernLights-557 Donkey Anti-Mouse IgG, Cross-adsorbed NorthernLights-637 NorthernLights-493 NorthernLights-557 Donkey Anti-Sheep IgG, Cross-adsorbed NorthernLights-637 NorthernLights-493 NorthernLights-557 Goat Anti-Rat IgG NorthernLights-637 NorthernLights-493 NorthernLights-557 Goat Anti-Chicken IgY NorthernLights-637 NorthernLights-493 Streptavidin NL493 Streptavidin NL637 Streptavidin NL557 NorthernLights-493 NorthernLights-637 NorthernLights-557 CATALOG # NL001 NL002 NL003 NL004 NL005 NL006 NL007 NL008 NL009 NL010 NL011 NL012 NL013 NL014 NL015 NL016 NL017 NL018 NL997 NL998 NL999 SIZE 500 L 500 L 500 L 500 L 500 L 500 L 500 L 500 L 500 L 500 L 500 L 500 L 500 L 500 L 500 L 500 L 500 L 500 L 500 L 500 L 500 L PRICE $115 $115 $115 $115 $115 $115 $115 $115 $115 $115 $115 $115 $115 $115 $115 $115 $115 $115 $115 $115 $115
ANTIBODY CONTROLS
CONTROL LABEL Fluorescein PE Goat IgG PerCP None Biotin Human IgG1 Fc Human IgG None None None Sheep IgG APC Fluorescein TYPE Normal goat IgG Normal goat IgG Normal goat IgG Normal goat IgG Normal goat IgG NS0-expressed recombinant protein Normal human IgG Normal sheep IgG Normal sheep IgG Normal sheep IgG CATALOG # IC108F IC108P IC108C AB-108-C BAF108 110-HG-100 1-001-A 5-001-A IC016A IC016F SIZE 200 Tests 200 Tests 200 Tests 1 mg 100 g 100 g 1 mg 1 mg 200 Tests 200 Tests PRICE $165 $165 $165 $75 $75 $325 $75 $75 $165 $165
110
IHC PROTOCOL GUIDE

ANTIBODY CONTROLS, continued
CONTROL LABEL PerCP APC Mouse IgG1 None Biotin Fluorescein PE None None PerCP Mouse IgG2A APC Biotin Fluorescein PE Mouse IgG2A Fc None None None None Mouse IgG2B APC Fluorescein PE PerCP None Mouse IgG3 APC Fluorescein PE APC Rabbit IgG Fluorescein PE None Rat IgG None None APC Rat IgG1 Biotin Fluorescein PE None APC Rat IgG2A Biotin Fluorescein PE PerCP PerCP APC Rat IgG2B Biotin Fluorescein PE None Mouse IgG3 Mouse IgM Chicken IgY PerCP PerCP None TYPE Normal mouse IgG1, clone # 11711 Normal mouse IgG1, clone # 11711 Normal mouse IgG1, clone # 11711 Normal mouse IgG1, clone # 11711 Normal mouse IgG1, clone # 11711 Normal mouse IgG1, clone # 11711 Normal mouse IgG2B, clone # 20102 Normal mouse IgG2B, clone # 133304 Normal mouse IgG2B, clone # 20102 Normal mouse IgG2B, clone # 20102 Normal mouse IgG2B, clone # 20102 Normal mouse IgG2B, clone # 20102 Normal mouse IgG2B, clone # 20102 NS0-expressed recombinant protein Normal mouse IgG2B, clone # 20116 Normal mouse IgG2B, clone # 133303 Normal mouse IgG2BB, clone # 73009 Normal mouse IgG2B, clone # 133303 Normal mouse IgG2B, clone # 133303 Normal mouse IgG2B, clone # 133303 Normal mouse IgG2B, clone # 133303 Normal mouse IgG3, clone # 133316 Normal Mouse IgG3, clone # 133316 Normal Mouse IgG3, clone # 133316 Normal Mouse IgG3, clone # 133316 Normal rabbit IgG Normal rabbit IgG Normal rabbit IgG Normal rabbit IgG Normal rat IgG Normal rat IgG1, clone # 43414 Normal rat IgG1, clone # 43414 Normal rat IgG1, clone # 43414 Normal rat IgG1, clone # 43414 Normal rat IgG1, clone # 43414 Normal rat IgG2A, clone # 54447 Normal rat IgG2A, clone # 54447 Normal rat IgG2A, clone # 54447 Normal rat IgG2A, clone # 54447 Normal rat IgG2A, clone # 54447 Normal rat IgG, clone # 54447 Normal rat IgG, clone # 141945 Normal rat IgG2B, clone # 141945 Normal rat IgG2B, clone # 141945 Normal rat IgG2B, clone # 141945 Normal rat IgG2B, clone # 141945 Normal rat IgG2B, clone # 141945 Normal mouse IgG, clone # 133316 Normal mouse IgM Normal chicken IgY CATALOG # IC002C IC002A MAB002 IC002B IC002F IC002P MAB003 MAB0031 IC003C IC003A IC003B IC003F IC003P 4460-MG-100 MAB004 MAB0041 MAB0042 IC0041A IC0041F IC0041P IC0041C MAB007 IC007A IC007F IC007P IC105A IC105F IC105P AB-105-C 6-001-A MAB005 IC005A IC005B IC005F IC005P MAB006 IC006A IC006B IC006F IC006P IC006C IC013C IC013A IC013B IC013F IC013P MAB0061 IC007C IC015C AB-101-C SIZE 200 Tests 200 Tests 500 g 200 Tests 200 Tests 200 Tests 500 g 500 g 200 Tests 200 Tests 200 Tests 200 Tests 200 Tests 100 g 500 g 500 g 500 g 200 Tests 200 Tests 200 Tests 200 Tests 500 g 200 Tests 200 Tests 200 Tests 200 Tests 200 Tests 200 Tests 1 mg 1 mg 500 g 200 Tests 200 Tests 200 Tests 200 Tests 500 g 50 g 50 g 200 Tests 200 Tests 200 Tests 200 Tests 200 Tests 200 Tests 200 Tests 200 Tests 500 g 200 Tests 200 Tests 1 mg PRICE $165 $165 $170 $165 $165 $165 $170 $170 $165 $165 $165 $165 $165 $325 $170 $170 $170 $165 $165 $165 $165 $170 $165 $165 $165 $165 $165 $165 $75 $75 $170 $165 $165 $160 $165 $170 $165 $165 $165 $165 $165 $165 $165 $165 $165 $165 $170 $165 $165 $75
111
IHC PROTOCOL GUIDE

Staining Kits & Reagents for Cells & Tissues Cell and Tissue Staining Kits are intended for localization of antigens in a broad range of histological and cytological specimens. These kits are based on the formation of the Avidin-Biotin Complex (ABC) with primary antibodies that react with the tissue antigens under study. The kits are made in ready-to-use format including prediluted Secondary Biotinylated Antibodies, and HSS-HRP that eliminates the need for extra steps in the procedure, minimizes hands-on time, maximizes convenience, and reduces the risk of erroneous calculations. Cell & Tissue Staining Kits
SPECIES Anti-goat Anti-mouse Anti-rabbit Anti-rat Anti-sheep Anti-goat Anti-mouse Anti-rabbit Anti-rat Anti-sheep LABEL HRP-DAB System COMPONENTS Secondary Biotinylated Antibody, Streptavidin-HRP Conjugate, DAB Chromogen, DAB Chromogen Buffer, Blocking Reagents CATALOG # CTS008 CTS002 CTS005 CTS017 CTS019 CTS009 CTS003 CTS006 CTS018 CTS020 SIZE 50 Tests PRICE $240
HRP-AEC System
Secondary Biotinylated Antibody, Streptavidin-HRP Conjugate, AEC Chromogen, AEC Chromogen Buffer, Blocking Reagents
50 Tests
$240
PRODUCT p27/Kip1 IHC Detection Kit
DESCRIPTION Includes reagents for antigen retrieval, blocking reagent, detection reagents, and a specific monoclonal primary antibody for human p27 antigen
p27 in Human Prostate Cancer. p27 was detected in paraffin-embedded human prostate cancer sections using the Human p27 Immunohistochemistry Detection Kit (Catalog # CTS2256). Tissue was stained using anti-mouse HRP-DAB (brown) and counterstained with hematoxylin (blue).
CATALOG # CTS2256
SIZE 50 Tests
PRICE $425
Cell & Tissue Staining Reagents

PRODUCT Basic Antigen Retrieval Reagent Acidic Antigen Retrieval Reagent Universal Antigen Retrieval Reagent Antigen Retrieval Reagent Sampler Avidin-Fluorescein Streptavidin-Allophycocyanin Streptavidin-Phycoerythrin Streptavidin-Alkaline Phosphatase DAB Enhancer DESCRIPTION Antigen retrieval system that utilizes a heat-induced recovery of cell and tissue antigens. Antigen retrieval system that utilizes a heat-induced recovery of cell and tissue antigens. Antigen retrieval system that utilizes a heat-induced recovery of cell and tissue antigens. All three reagents (CTS013, CTS014, CTS015) in one convenient pack. For use as a secondary reagent in immunofluorescent assays in conjunction with biotinylated primary labeling reagents. For use as a secondary reagent in immunofluorescent assays in conjunction with biotinylated primary labeling reagents. For use as a secondary reagent in immunofluorescent assays in conjunction with biotinylated primary labeling reagents. For use in ELISA or Western blot assays. DAB Enhancer is a concentrated 50X metal based solution used to intensify the DABChromogen (Catalog Numbers # CTS002, CTS005, CTS008, CTS017). In the presence of the DAB Enhancer, the DAB Chromogen produces a clear brown-black staining with minimal background. Permanent aqueous mounting medium CATALOG # CTS013 CTS014 CTS015 CTS016 F0030 F0050 F0040 AR001 CTS010 SIZE 50 mL 50 mL 50 mL 50 mL 100 Tests 100 Tests 100 Tests 1 mL 3 mL PRICE $60 $60 $60 $120 $65 $85 $75 $145 $45
Mounting Medium
CTS011
15 mL
$35
Control
Antigen Retrieval
Antigen Retrieval Improves Detection of Neuroplastin 65. Neuroplastin 65 was detected in human cerebellar tissue using anti-human/mouse Neuroplastin 65 affinity-purified polyclonal antibody (Catalog # AF5360). Tissue was pretreated with Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the anti-Goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
112
Ordering Information
CuSTOMERS IN uSA & CANADA
Online ordering is available for customers in the U.S.A. and Canada. After finding your products, click the shopping cart symbol to create an account or to make an online purchase.
Website: www.RnDSystems.com Phone: Fax: Mail: 1-800-343-7475 1-612-656-4400 R&D Systems, Inc. 614 McKinley Place NE Minneapolis, MN 55413
INTERNATIONAL DISTRIBuTORS
Please visit www.RnDSystems.com for a full list of international distributors.
FOR ALL PuRCHASES, PLEASE INCLuDE THE FOLLOWING INFORMATION WITH yOuR ORDER
Name & Contact Information Name of Institution Delivery Address Invoice Address Purchase Order Number* or Credit Card Number Quantity and Product Size Product Catalog Number and Description Name of Principal Investigator (optional) Telephone Number E-mail Address (optional) For European Customers: VAT Exemption Certificate (UK) or E.C. equivalent * New customers wishing to use a P.O. number, please call customer service at 1-800-343-7475 to set up an account.
New IHC/ICC Screensavers!
To download R&D Systems IHC/ICC image screensavers, please visit our website at: www.RnDSystems.com/go/Screensavers
USA & Canada R&D Systems, Inc. 614 McKinley Place NE Minneapolis, MN 55413, USA TEL: (800) 343-7475 (612) 379-2956 FAX: (612) 656-4400 E-MAIL: info@RnDSystems.com www.RnDSystems.com
Europe R&D Systems Europe Ltd. 19 Barton Lane, Abingdon Science Park Abingdon OX14 3NB, UK TEL: +44 (0)1235 529449 FAX: +44 (0)1235 533420 E-MAIL: info@RnDSystems.co.uk www. RnDSystems.com
China R&D Systems China Co., Ltd. 24A1 Hua Min Empire Plaza 726 West Yan An Road, Shanghai, PRC 200050 TEL: +86 (21) 52380373 FAX: +86 (21) 52371001 E-MAIL: info@RnDSystemsChina.com.cn www.RnDSystemsChina.com.cn
International Distributors Please visit www.RnDSystems.com/go/Distributors for a full list of international distributors.
CA103_IHCprotocol_MAR

IHC Protocol Guide

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IHC Protocol Guide

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R&D Systems Tools for Cell Biology Research

IHC Products & Protocol Guide

Catalog # AF3970 Vanilloid R1 in Rat Dorsal Root Ganglion

Catalog # AF928 IL-2 Receptor a in Mouse Spleen

Catalog # AF3066 Notch-2 in Rat Choroid Plexis

Catalog # AF2418/MAB1326 STAT6 in Human Peripheral Blood Mononuclear Cells (PBMCs)

Catalog # AF2438 Glutathione Peroxidase 1 (GPX1) in Rat Liver

Catalog # AF1190 MESDC2 in Adult Mouse Testis

Catalog # MAB2167 Meprin b Subunit in Mouse Intestine

Catalog # AF3798 GFAP in Rat Astrocytes

VARIABLES FOR EXPERIMENTAL DESIGN & OPTIMIZATION

For research use only. Not for use in diagnostic procedures.

IHC SAMPLE PREPARATION COMPARISON

Cytospin is a registered trademark of Thermo Electron Corporation.

Fixation of Cultured Cells

For research use only. Not for use in diagnostic procedures.

Preventing Non-specific Hydrophobic Interactions

Endogenous Enzyme Interference

Endogenous Biotin Interference

Preventing Non-specific Ionic Interactions

Selecting a Primary Antibody Supplier

Optimization of Primary Antibody Incubation

Selecting a Monoclonal or Polyclonal Antibody

Affinity Purification of Polyclonal Antibodies

STARTING CONDITIONS FOR PRIMARY ANTIBODY INCUBATION

For research use only. Not for use in diagnostic procedures.

Direct or Indirect Detection, & Amplification

DETECTION METHODS & SIGNAL INTENSITY

ADVANTAGE Good for multicolor labeling INDIRECT

LIMITATION Lower sensitivity

ADVANTAGE Higher sensitivity

LIMITATION Potential non-specific binding of secondary antibodies AMPLIFICATION ABC METHOD

ADVANTAGE Increased signal intensity

LIMITATION Requires additional time & controls

NorthernLights is a trademark of R&D Systems.

For research use only. Not for use in diagnostic procedures.

Endogenous Tissue Background Control

No Primary Antibody Control

Tissue Type Control

Limitations of Western Blot Comparisons

Antigen Retrieval Techniques

Protease-induced Epitope Retrieval (PIER)

Heat-induced Epitope Retrieval (HIER)

Optimization & Limitations

For research use only. Not for use in diagnostic procedures.

BLOCK PRIMARY ANTIBODIES SECONDARY ANTIBODIES LABEL COUNTERSTAIN COVERSLIP

RAGE in Alzheimers Brain.

Meteorin in Rat Cortical Stem Cells.

40 g Paraformaldehyde Powder 1 N NaOH UNTIL POWDER DISSOLVES

HEAT WHILE STIRRING

ADJUST THE pH TO 6.9 WITH DILUTE HCl ALIQUOT & FREEZE

Formaldehyde Structure. In an aqueous solution, formaldehyde exists primarily as methylene glycol.

For research use only. Not for use in diagnostic procedures.

5 g Gelatin AFTER THE GELATIN DISSOLVES ADD 0.5 g CrK(SO4)2

HEAT AT 45 C WHILE STIRRING

COOL & FILTER AT ROOM TEMPERATURE

ADD Gelatin-coating Solution

DIP RACKS WITH SLIDES 3 to 5 TIMES, 5 SECONDS EACH

DRY AT ROOM TEMPERATURE FOR 48 HOURS

STORE AT ROOM TEMPERATURE OR AT -20 C IF INTENDED FOR CRYOSTAT SECTIONS

CRYOSTAT SECTIONS THAW SLIDES AT ROOM TEMPERATURE 10-20 MINUTES

PARAFFIN-EMBEDDED TISSUE SECTIONS