erm ne ae ee a tee evaluation and improvement” ee ey ISTA Seed Symposium, 2004 ABSTRACTS 00g Se ee ea 17-19 May, 2004 Budapest, Hungary—_ Budapest, Hungary, May 17" - 19", 2004 to germinate. The main objective of this study was to determine the best treatment for germination and dormancy elimination in laboratory on following plants: Calendula officinalis, Lavandula angustifolia, Echinacea purpurea, Chrysanthemum Ginerrariae folium and Nepta caturia. This research was conducted base on CRD with 4 replications in seed laboratory using following treatments GAs, KNOs, stratification, physical and chemical scratch. The results showed that there are significant differences between treatments, some of those plants had good germination under GA; treatments and some of them had good germination under KNO3 treatment and some of them had germination under stratification treatment and finally using suitable treatment has led dormancy elimination also promote germination and finally guarantee the growth of seedling in field. 93 - THE USE OF THE ACCELERATED AGING TEST FOR ESTIMATING THE POTENTIAL OF SEEDLING EMERGENCE IN THE FIELD OF SOYBEAN SEED Lots FRANCA-NETO, J.B.', KRZYZANOWSKI, F.C’, PADUA, G. HENNING, A.A." & COSTA, NP." ‘embrapa Soybean, Caixa Postal 231, CEP 86001-970, Londrina, PR, Brazil; franca@enpso.embrapa.br; *EMBRAPAYEPAMIG, Caixa Postal 351, CEP 38001-970 Uberabe, MG, Brazil The accelerated aging test (AAT) is used for estimating a vigour index of seed lots and also for estimating their storage potential. For soybean seeds an exposure period of 72 h at a constant temperature of 419C and 100% RH is recommended by ISTA. However very few studies which relate the results of AAT with soybean seedling emergence in the field (SEF) are available in the literature. Preliminary results obtained by Embrapa Soybean indicated that the AAT could be used for estimating SEF, if the test is applied to soybean seeds just before planting time, and the stress period is reduced to 24 h. The objective of this study was to verify the possibility of using the data of vigour provided by the AAT (41°C/100%RH/24h) for predicting the potential of soybean SEF. Regression analyses were obtained with the results of vigour provided by the AAT with the ones of SEF during the period of five years, from 1998 to 2002, A total of 879 seed samples of different cultivars were evaluated in the study. In each of the study year the AAT with an exposure period of 24 h was performed in October and in November (normal planting period for soybeans in Brazil) the evaluations o SEF were performed, All seed samples were treated with fungicide before planting in the field. The evaluations of SEF were done on the experimental field of Embrapa Soybean, in Londrina, Brazil. The regression analyses provided adjusted equations between the results of SEF and AAT. These analyses were individually evaluated for each study year and in conjunction for the five-year period, Comparing the individual equations obtained each year, It was observed that they were similar to each other. The conjunction analysis for the five-year period provided a regression equation between SEF and AAT (SEF = 0,6316AAT + 28,922; r2 = 0,83 ***), which was also similar to the individual equations obtained each year. This equation was applied to all AAT results of 100 seed samples evaluated in 2002. ‘The values of SEF estimated by this equation were correlated with the actual recorded data of SEF, resulting in a coefficient of regression of 0.80, which was highly significant (P < 0.001). This equation was considered reliable for estimating SEF of soybean seed samples in sols of Londrina, with soll temperature and moisture levels near to Ideal conditions. 94 - THE USE OF THE TETRAZOLIUM TEST FOR PREDICTING THE POTENTIAL OF SEEDLING EMERGENCE OF SOYBEAN SEED LOTS FRANCA-NETO, J.5.', KRZYZANOWSKL, F.C', PADUA, G.P.2, HENNING, A.A." & COSTA, N.P.* "Embrapa Soybean, Caixa Postal 231, CEP 8601-970, Londrina, PR, Brazil; franca@cnpso.embrapa.br; AEMBRAPAYVEPAMIG, Caixe Postal 351, CEP 3601-970 Uderaba, MG, Brazil The tetrazolium test (TTZ) provides a number of seed quality parameters, such as vigour (TZ-Vig), viability (TZ-Viab), and indexes of mechanical damage, field weathering, and stinkbug damage. The soybean seed-producing sector has demanded research studies that relate the results obtained by this test with the potential seedling emergence in the field (SEF). This type of information will greatly contribute for increasing the precision of soybean ael 27th ISTA Congress ~ Seed Symposium seed plantability. Additionally it will improve seed quality evaluation just before they are commercialised, The objective of this study was to verify the possibility of using the data of viability and vigour provided by the TTZ for predicting the potential of soybean SEF. Regression analyses were obtained with the results of vigour and viability provided by the TTZ with the ones of SEF during the period of six years, from 1997 to 2002. A total of 1,117 seed samples of different cultivars were evaluated in the study. In each of the study year the TZ was performed in October and in November (normal planting period for soybeans in Brazil) the evaluations 0 SEF were performed. All seed samples were treated with fungicide before planting in the field. The evaluations of SEF were done on the experimental field of Embrapa Soybean, in Londrina, Brazil. The regression analyses provided adjustec equations between the results of SEF and Z-Vig and Tz-Viab. The regression analyses were individually evaluated for each study year and in conjunction for the six-year period. ‘Comparing the individual equations obtained each year, it wes observed that the ones relating SEF and TZ-Vig were similar to each other. However this trend was not observed for the data relating SEF and TZ-Viab. This fact means that the TZ-Vig data is more reliable than those from TZ-Viab for estimating of SEF. The conjunction analysis for the six-year period provided regression equations between SEF and Tz-Vig and also TZ-Viab. Due to the fact that the Individual equations for each year were more coherent and repeatable for TZ-Vig than for TZ-Viab, it was concluded that SEF is more precisely estimated using TZ-Vig information as reference. The obtained equation for the six-year period involving SEF and TZ-Vig (SEF = 0,6165TZ-Vig + 35,716; 12 = 0,73 ***) was applied to all TZ-Vig of 100 seed samples evaluated in 2002. The values of SEF estimated by this equation were correlated with the actual recorded data of SEF, resulting in a coefficient of regression of 0.79, which was highly significant (P < 0.001). This equation was considered reliable for estimating SEF of soybean seed samples in soils of Londrina, with soil temperature and moisture levels near to ideal conditions. 95 - EFFECTS OF HYDRATION AND DEHYDRATION PERIODS LENGTH ON THE COLEOPTILES AND ROOT GROWTH AND SEEDLING SURVIVAL OF 3 WHEAT CULTIVARS AFTER REHYDRATION GHORBANI, M.H., ZEINALI, E, & SOLTANI., A. Dept. of Agron., Gorgan Univ. of Agric. Sci., Gorgan., Iran, Email: ghorbani304@yahoo.com Wheat (Triticum aestivum L.) seedlings may meet dehydration between seed imbibition and emergence, particular in dryland farming. This laboratory experiment was conducted to study the effects of hydration and dehydration periods length on the coleoptile and root growth, seedling survival, and their ability to resume growth (after rehydration) of 3 wheat cultivars. Seeds of Golestan. Khazar-1 and Tajan cultivars were germinated for 4, 6 and 8 days in suitable moisture conditions. Then, seedlings were dehydrated for 2, 4 and 6 days. Number of normal seedling, lenaths of coleoptiles and root, and dry weights of seed residues Were measured after 7 days. Results showed that when seedlings were grown under suitable moisture conditions for 4 days, dehydration periods had no effect on the number of normal seedlings after rehydration, but an increase in the time of dehydration resulted to decrease the coleoptile and root length of 3 cultivars. Dehydration reduced normal seedlings and root lengths, significantly, when seedlings were placed under normal conditions before dehydration for 6 days or more. Reduction in coleoptile lengths as affected by dehydration wes significant only for the treatment 8 days growth under suitable moisture conditions before dehydration. Dehydration period length had no effect on dry weights of seed residues in treatment 4 days growth under suitable moisture conditions before dehydration, while when seedlings were grown under suitable conditions for 6 days, increasing dehydration time followed by significant increase in dry weights of seed residues. In 8 days growth under suitable conditions before dehydration, increasing dehydration period length caused significant increase in seed residues only in Golestan cultivar. These results demonstrate that dehydration tolerance reduces as wheat seedlings develops. On the other hand, the length of dehydration can be effective an the ability of regrowth after rehydration. So this ability decreases as the length dehydration period increases 74