List of Slides for The Practical Exam

1). Cardiac Muscle (H & E)

Slide #5:
Heart Wall; Cross Section
Visible at the top of the photo are the Perkingy Fibers (large, “lumpy-looking”
fibers), responsible for causing the contractions of the heart.
Note: The nuclei of heart muscle cells is at their periphery as well
H & E Staining
(Low Magnification)
Slide #5:
Heart Wall; Cross Section
Visible at the top of the photo are the Perkingy Fibers (large, “lumpy-looking”
fibers), responsible for causing the contractions of the heart.
 Beneath the Perkingy Fibers are visible the Cardiac Muscle Cells, and at the periphery
of these cells, stained a very definitive purple are the nuclei.
H & E Staining
(Medium Magnification)

2). Blood Smear (May-Grunwald-Giemsa Staining)

Blood Smear
May-Grünwald-Giemsa Staining
Neutrophil, a.k.a Polymorphonuclear Leukocytes (with 4 nuclei; depicting its
“old” status). Pink Cytoplasm.
Surrounding the Neutrophil, many Red Blood Cells (anucleated, of
course)
(High Magnification)
Blood Smear
May-Grünwald-Giemsa Staining
Neutrophil, a.k.a Polymorphonuclear Leukocytes (with 2 nuclei; depicting its
“young” status, as compared to the previous picture). Pink Cytoplasm.
(High Magnification)

TV Screen:
Blood Smear
Eosinophil. The DEEPLY/INTENSELY RED STAINED granulocytes cover
most of the cytoplasm and make this cell easily recognizable.
The Nucleus also has only 2 lobes.
May-Grünwald-Giemsa Staining
(High Magnification)

TV Screen:
Blood Smear
Eosinophil. The DEEPLY/INTENSELY RED STAINED granulocytes cover
most of the cytoplasm and make this cell easily recognizable.
The Nucleus also has only 2 lobes
May-Grünwald-Giemsa Staining
(High Magnification)

TV Screen:
Blood Smear
 Basophil. Slight “pinky” border with dark blue granules COVERING THE
ENTIRE CYTOPLASM. The cell is so internally granular that the nucleus is
invisible.
It is easy to differentiate between Basophils and other white blood cells
due to its granularity.
May-Grünwald-Giemsa Staining
(High Magnification)

TV Screen:
Blood Smear
Monocyte. Monocytes are MUCH LARGER THAN Neutrophils, and their
kidney-bean shaped nuclei make them easily distinguishable
May-Grünwald-Giemsa Staining
(High Magnification)
TV Screen:
Blood Smear
Lymphocyte. Very small sized. Dark coloured cytoplasm with a small rim of
pink-coloured cytoplasm. These cells are the B cell and the T cells.
May-Grünwald-Giemsa Staining
(High Magnification)

TV Screen:
Blood Smear
Platelets. Seen in this photograph in the left part of the TV screen. Responsible
for blood-clotting.
May-Grünwald-Giemsa Staining
(High Magnification)

TV Screen:
Blood Smear
Eosinophil (bottom & towards the left) and Neutrophil (atop the Eosinophil).
Easily distinguishable due to the Eosinophils intense colour in the
cytoplasm, and due to the Neutrophils multiple nuclei and pink cytoplasm.
May-Grünwald-Giemsa Staining
(High Magnification)

3). Lymph Node (H & E)

Slide #1:
Lymph Node
Visible in this photograph are the lymphatic outer sinuses (spaces within lymph nodes), as well as
within the medulla, the medullary sinuses are visible
The dense purple tissue is the cortex
The majority of what is visible in this photo is the dark purple cortex
 The resident cells (T Cells) are purple within the lymph nodules
 The pink stained areas are merely connective tissue
H & E Staining
(Low Magnification)

Slide #1:
Lymph Node
In this photograph, prominently visible, are the cortex and lymphatic zones.
H & E Staining
(High Magnification)

4). Palatine Tonsil (H & E)

Slide #3:
Palatine Tonsil
Very visible in this photograph are the dark purple stained lymphocytes, and the
“round-shaped” structures are the arteries with their very thick walls. At the
lower left 1/3 of the photograph are visible the veins, which appear as elongated
structures.
H & E Staining
(High Magnification)

5). Lingual Tonsil (H & E)

Slide #6:
Lingual Tonsil
Visible in this photograph are: Mucous glands (left of image), and directly below
them is visible a very large vein (orangy-red colour). As well, dark-purple stained
region is the germinal center, within its periphery which are visible several
arteries (to its left). Furthermore, the elongated-appearing structures which have
the reddish staining are the venuoles.
H & E Staining
(Low Magnification)

6). Spleen (H & E)

Slide #2:
Spleen
The Capsule, trabeculae, trabecular veins and arteries, white pulp (PALS,
lymphatic nodules), red pulp (splenic cords and sinuses), and pulp veins are
visible.
--The pulp veins are visible here as the most easily visible, reddish circular
objects within the white pulp
--The capsule is visible encircling the periphery
--The sinusoids are MUCH smaller than the pulp veins, yet also have the
same colour as the veins. In this picture, the sinusoids are dispersed
throughout the image, with several concentrated near the capsule
H & E Staining
(Medium Magnification)
Slide #2:
Spleen
At the top of the image is visible the capsule, which appears as a band at the top of the photo.
As well, prominently visible in this photo is the “pulp vein” in the center of the
image, with its deep red colour. As well, several dark purple lymphatic nodules
are visible.
 NOTE: Lymphatic nodules ONLY appear during an immune
response!
H & E Staining
(Medium Magnification)
Slide #2:
Spleen
Prominently visible in this photo is the “pulp vein” in the center of the image,
with its deep red colour. As well, several dark purple lymphatic nodules are
visible.
 NOTE: Lymphatic nodules ONLY appear during an immune
response!
H & E Staining
(Medium Magnification)

Slide #2:
Spleen
The most prominent feature of this image is the Trabeculae, which can be seen
here branching and stemming from the right of the image. Within its center can
be seen the trabecular artery.
H & E Staining
(Medium Magnification)

7). Thymus-Juvenile (H & E)

Slide #4:
Thymus-Juvenile
The light pink stained regions are the Medulla (Mature Thymocytes present); –ve Selection here
The dark purple stained regions are the Cortex (Immature Thymocytes present); +ve Selection here
 The lymphoid nodules are easily identifiable in the Cortex as several dark purple
regions
H & E Staining
(Low Magnification)

TV Screen:
Thymus-Juvenile
 In this middle of this photo are visible a couple of easily identifiable Hassal’s
Corpuscles (“Hassal Bodies”)
---Hassal Bodies, ONLY present in the Medulla
 Surrounding the Hassal’s Corpuscles, the Cortex
H & E Staining
(Low Magnification)

Slide #4:
Thymus-Juvenile
In this picture is an easily identifiable Hassal’s Corpuscles (Hassal Body)
---Hassal Bodies, ONLY present in the Medulla
---Reticular cells can also be seen in the vicinity of the interior of
the Hassal Body
H & E Staining
(High Magnification)

8). Thymus in Involution (H & E)

Slide #5:
Thymus in involution
Involution: A progressive decline or degeneration of normal physiological
functioning occurring as a result of the aging process
The white globular cells are adipose tissue
The dark concentric regions within the cortex are hyalized cells
 Note: The cortex is the dark purple stained regions (right of photo) containing the immature
thymocytes
 Note: The medullary region contains the mature thymocytes (center of photo), with the hyalized
cells within it
H & E Staining
(Low Magnification)
Slide #5:
Thymus in involution
H & E Staining
(High Magnification)

9). Thyroid Gland with Parathyroids (H & E)

Slide #4:
Thyroid and Parathyroid Gland
 At the centre of this image is a typical colloid cell, and surrounding it in a disk are the
Thyroid Follicular Cells. Around the Thyroid Follicular Cells lies the Basal Lamina.
 Although H & E DOESN’T stain for it and they are not readily visible in this image,
Parafollicular cells (“C” cells; calcitonin producing) lie around the Thyroid Follicular
Cells.
H & E Staining
(High Magnification)

Slide #4:
Thyroid and Parathyroid Gland
 Visible in this photo are the paraffin-embedded Adipose Tissue
H & E Staining
(High Magnification)

10). Adrenal Gland (H & E)

Slide #2:
Adrenal Gland
In this image are visible large PURPLE stained nuclei (i.e., similar staining pattern to Lymphocyte
nuclei because the nucleus to cell size ratio is high in both these types of cells).
H & E Staining
(High Magnification)

Slide #2:
Adrenal Gland
Visible from the bottom left of the image to the top right of the image are the 3 regions of the gland:
1). Zona Glomerulosa
2). Zona Fasciculata
 3). Zona Reticularis
Note: There is also adrenal medulla as well.
H & E Staining
(Low Magnification)

Slide #2:
Adrenal Gland
From Right to Left in this image are:
The Capsule and 1). Zona Glomerulosa and 2). Zona Fasciculata
H & E Staining
(Low Magnification)

11). Supraoptic and paraventricular nuclei of the hypothalamus (Gomori Staining)

 NOTE: Supraoptic tested ONLY!
Slide #P:
Supraoptic and paraventricular nuclei of the hypothalamus
Visible in this photo: Supraoptic Nuclei
Gomori Staining
(High Magnification)
Slide #P:
Supraoptic and paraventricular nuclei of the hypothalamus
Visible in this photo: Paraventricular Nuclei (look “above and below” to the left of the raphe/slit in
the middle of the image. The cluster of nuclei there are the paraventricular
nuclei)
Gomori Staining
(High Magnification)

12). Hypophysis (Mallory Staining)

Slide #1:
--Microscopic View
Hypophysis
Visible in this photo: 1). Acidophiles (Reddish-orange stained), 2). Basophiles (Blue stained), and
3). Connective tissue (stained blue)
Visible as well: 4). “Chromophobes”: appear as empty “white” spaces
(Chrom: colour phobes: “haters”)
These cells secrete: Vasopressin and Oxytocin
Mallory Staining
(Low Magnification)

13). Nonhairy skin-longitudinal section (H & E)

Slide #2:
Nonhairy Skin-longitudinal section
Visible in this photo are several layers of the skin (from top left to bottom right): The 1). stratum
corneum (the top layer), 2). stratum lucidum (red band), 3). stratum granulosum (dark purple band
beneath the stratum lucidum), 4). stratum spinosum, and 5). stratum basale. The 6). connective
tissue is beneath the stratum basale. The visible “grooves” between the stratum basale and the
connective tissue provide for a more secure lock-and-key mechanism between the two layers.
H & E Staining
(Medium Magnification)

14). Hairy Skin-longitudinal section (H & E)

Slide #1:
Hairy Skin-longitudinal section
Visible in this photo are (from follicle out): The cortex, melanocytes (black specks at bulb of hair)
with dermal papilla just inferior to them, the internal root sheath and the external root sheath, and the
outside layer, the glossy membrane.
H & E Staining
(Medium Magnification)

Slide #1:
TV Screen:
Hairy Skin-longitudinal section
 Prominently visible in the central part of this photo, a sebaceous gland.
H & E Staining
(High Magnification)
Slide #1:
Hairy Skin-longitudinal section
Visible in this photo are (from follicle out): The cortex, melanocytes (black specks at bulb of hair)
with dermal papilla just inferior to them, the internal root sheath and the external root sheath, and the
outside layer, the glossy membrane.
H & E Staining
(High Magnification)

Slide #1:
Hairy Skin-longitudinal section
 Visible in the centre of this photo as a coiled structure with black-“specks”, is the sweat gland
H & E Staining
(High Magnification)

15). Hairy Skin-cross section (H & E)

Slide #2:
Hairy Skin-cross section
Seen in the centre of the photo are (from medulla outwards): The medulla, cortex, cuticle, internal
root sheath, and external root sheath.
H & E Staining
(Low Magnification)

16). Lip (H & E)

Slide #1:
Lip
Visible in this photo are: The Vermillion Zone (far right, subsurface layer pink-
coloured), sebaceous gland, muscle tissue, adipose tissue, and an artery.
H & E Staining
(Low Magnification)

17). Tongue-filiform and fungiform papillae (H & E)

 Note: Fungiform NOT tested

Taste Buds
Foliate Papillae
Note: The Tall structure of the Papillae

Fungiform Papillae
Note: Mushroom Appearance

18). Tongue-filiform and foliate papillae and taste buds (H & E)

Filiform Papillae
Note: These taste buds are VERY shallow and easy to recognize

19). Developing tooth (Mallory staining)

Slide #2:
Developing Tooth
Visible in this photo are (from far right to left): Pulp, Odontoblasts (secrete
Dentin), Predentin, Dentin (with the thick, rich red canaliculae), Enamel, and to
the far left, Ameloblasts=adamentoblasts (secrete enamel).
H & E Staining
(Low Magnification)

20). Adult tooth (Mallory Staining)

Slide #Z:
Adult Tooth-Decalcified
 Decalcification has dissolved the enamel of this tooth
Mallory Staining
(Low Magnification)

21). Parotid Gland (H & E)

Parotid Gland
(Low Magnification)

Parotid Gland
Note: Invaginations of the epithelium!!!
Low Magnification
Slide #15:
Parotid Gland
The duct is prominently visible in the centre of this photo
H & E Staining
(High Magnification)

22). Sublingual gland (H & E)

Sublingual gland
Note: Many mucous tubules; easy to distinguish between sublingual and submandibular
23). Submandibular Gland (H & E)

Slide #17:
Submandibular Gland
Ducts are visible at the centre of this photo
Note: Presence of mucous tubules and prevalence of DARK serous
elements
H & E Staining
(Low Magnification)

Submandibular Gland
Ducts are visible at the centre of this photo
 A great MIXTURE of MUCOUS TUBULES and STRIATED DUCTS
NOTE: Appears MUCH DARKER than sublingual gland as well!
H & E Staining

24). Esophagus (H & E)

Slide #6:
Esophagus
Visible at the the far right of this picture is the Stratified Squamous Epithelium
H & E Staining
(Low Magnification)
Slide #6:
Esophagus
Visible centrally located in this picture is the smooth muscles
H & E Staining
(Medium Magnification)

Slide #6:
Esophagus
Visible in the centre of this picture is an esophageal gland
H & E Staining
(Medium Magnification)
Slide #6:
Esophagus
Visible in this image are skeletal muscles
H & E Staining
(Medium Magnification)

25). Gastric fundus-staining for parietal cells (Kongo Red)

Slide #7:
Gastric Fundus
This slide has been stained for parietal cells
Kongo Red Staining
(Low Magnification)
Slide #7:
Gastric Fundus
 This slide has been stained for “Parietal Cells”
o The orange cells located peripheral to the main cells are the “Parietal Cells”
 The Parietal Cells are responsible for producing HCL
 The cells which the Parietal cells are surrounding are the “Chief Cells”
 The Chief Cells are responsible for producing Pepsinogen
Kongo Red Staining
(Low Magnification)

26). Gastric pylorus (H & E)

Slide #8:
Gastric Pylorus
Visible in this slide are (from far left to right): Muscularis Mucosae, the mucous secreting cells
located adjacent to the Muscularis Mucosae, and cells of the Gastric Pits (dark purple cells which
also produce mucous)
Note: The nerves in the Gastric Plexus. These are nerves of the Auerbach’s Plexus
H & E Staining
(Low Magnification)

Slide #8:
Gastric Pylorus
Visible in this slide is a Nerve passing through the gastric pylorus
Note: The nerves in the Gastric Plexus. These are nerves of the Auerbach’s Plexus
H & E Staining
(Medium Magnification)

27). Small intestine-duodenum (H & E)

Slide #9:
Small Intestine-duodenum
Key Point for Duodenum: Has the LONGEST villi of the 3 regions of the small intestine!
 Visible in this photo are (left to right): villi, the submucosal region with Brunner’s
Glands and the Meissner Plexus, and to the far right, the muscle of the duodenum
Although not visible, the duodenum also has Peyer’s Patches (Lymphoid nodules)
H & E Staining
(Low Magnification)

28). Small intestine-jejunum (H & E)

Slide #10:
Small Intestine-jejunum
Key Point for Jejunum: Has MEDIUM SIZED villi of the three regions, but not as long as
duodenum!
 Visible in this photo are (left to right): the Meissner Plexus and connective tissue, BUT
NO Brunner’s Glands present in Jejunum (contrast with Duodenum), and to the far right, the
villi.
Note: The layering of the duodenum, jejunum, and ileum are all the same, except that
in the jejunum, there are NO Brunner’s Glands and NO Peyer’s Patches in
jejunum, as opposed to the duodenum which DOES possess them.
H & E Staining
(Low Magnification)

29). Small intestine-ileum (H & E)

Small Intestine-ileum
Key Point for Ilium: Has SHORTEST villi of the three regions!
 Visible in this photo are (top to bottom): the SHORT VILLI, and Peyer’s Patches.
Note: The layering of the duodenum, jejunum, and ileum are all the same. The
differences are that the ileum has the SHORTEST VILLI and DOES HAVE
PEYER’S PATCHES.
H & E Staining

30). Large intestine-colon (H & E)

Slide #11:
Large Intestine-colon
H & E Staining
(Low Magnification)

31). Large intestine-appendix (H & E)

Slide #12:
Large Intestine-Appendix
H & E Staining
(High Magnification)
Large Intestine-Appendix
Note: The circular structure of the specimen, as well as the LARGE collection of lymphoid
nodules in the appendix
H & E Staining
(High Magnification)

32). Human liver (H & E)

Slide #1:
Human Liver
Centrally located in this photo is visible the Portal Space, with hepatocytes surrounding it
  At the top of the Portal Space is visible an artery (with its thick tunica media), and at the
centre of the portal space is visible a massive vein.
 At base of Portal Space is visible a Bile Duct (with blue stained central region).
 Directly above the bile duct is an artery, and to the right of the artery and below the
massive vein is a Lymph Vessel
 Liver also has Ito Cells: Control Vasoconstriction.
H & E Staining
(High Magnification)

33). Swine liver (H & E)

Slide #2:
Swine Liver
Visible in this photo: Top of picture is a Lymph Vessel
Bottom left of picture is the Portal Space
The interlying space between the two has many hepatocytes in it.
H & E Staining
(Medium Magnification)
Slide #2:
Swine Liver
Visible in this picture is a Liver Sinusoid
The purple stained regions are the hepatocytes, and within the liver sinusoids are visible
erythrocytes which are red coloured.
H & E Staining
(Medium Magnification)

Slide #2:
Swine Liver
Visible in this picture is a Liver Sinusoid
 The purple stained regions are the hepatocytes, and within the liver sinusoids are visible
erythrocytes which are red coloured.
H & E Staining
(High Magnification)

34). Human liver (Mallory staining)

Slide #2A:
Human Liver
Visible in the centre of this photograph is the liver sinusoid
Mallory Staining
(Medium Magnification)
Slide #2A:
Human Liver
At the centre of this photo is the Central Vein.
Surrounding the central vein are hepatocytes, which are all pointed towards the central vein
concentrically!
Mallory Staining
(Medium Magnification)

Slide #2A:
TV presentation
Human Liver
  Visible are the Portal space (within which are erythrocytes, the artery at base of it)
 To the right of portal space are visible Ito Cells (brown stained region)
Mallory Staining
(Medium Magnification)

35). Gall bladder (H & E)

Slide #10:
Gall Bladder
 Visible in this photograph are columnar epithelium (top layer), and beneath the columnar
epithelium is a smooth muscle layer. Directly beneath this layer is the serosa.
Note for the lab exam: The columnar epithelium folds in ALL DIRECTIONS.
Also, there are NO GLANDS in the mucosal layer.
H & E Staining
(Medium Magnification)

36). Pancreas (H & E)

Slide #3:
Pancreas
 Centrally visible in this photograph is an “Islet of Langerhans”
--Visible surrounding the Islet of Langerhans are the “Serous acinus,” which are exocrine
cells
H & E Staining
(Medium Magnification)

37). Pancreas (Brachet’s staining)

Brachet’s staining stains for RNA, and visible under this staining in this slide are RIBOPROTEINS
38). Trachea-cross section (H & E)

Slide #1:
Trachea
Visible in this photo are the pseudostratified epithelium
Note for the lab exam: LOTS OF HYALINE CARTILLAGE visible under the epithelium
H & E and PAS Staining
(High Magnification)

Slide #1:
Trachea
 Visible in this photo are: Veins (with dark “blotches” of endothelial cells surrounding them),
connective tissue, as well as the pseudostratified epithelium.
H & E and PAS Staining
(Medium Magnification)

39). Lung Parenchyma (H & E)

Slide #2:
Lung Parenchyma
Visible in this photo are: Bronchi, Bronchioles, and Alveoli, which are part of the Conducting
System of the Respiratory Tract. The terminal portion only has Single Alveoli, as opposed to the
Respiratory Portion which has several Alveoli. As well, BRONCHIOLES contain NO
CARTILLAGE, as opposed to BRONCHI which CONTAIN CARTILLAGE.
H & E Staining
(High Magnification)
Slide #2:
Lung Parenchyma
Visible in this photo are: Bronchi, Bronchioles, and Alveoli, which are part of the Conducting
System of the Respiratory Tract. The terminal portion only has Single Alveoli, as opposed to the
Respiratory Portion which has several Alveoli. As well, BRONCHIOLES contain NO
CARTILLAGE, as opposed to BRONCHI which CONTAIN CARTILLAGE.
H & E Staining
(Low Magnification)

Slide #2:
Lung Parenchyma
Visible in this photo are: Vein and Alveoli.
H & E Staining
(Low Magnification)

Slide #2:
Lung Parenchyma
Visible in this photo are: Bronchioles.
H & E Staining
(Low Magnification)

Slide #2:
Lung Parenchyma
 Visible in this photo are: Bronchioles. BRONCHIOLES, as visible here, contain NO
CARTILLAGE, as opposed to BRONCHI which CONTAIN CARTILLAGE.
H & E Staining
(Low Magnification)

Slide #2:
Lung Parenchyma
Visible in this photo are: Bronchi, and clearly visible CHONDROCYTES (forming
CARTILLAGE). As well, BRONCHIOLES contain NO CARTILLAGE, as opposed to BRONCHI
which are clearly visible here and which DO CONTAIN CARTILLAGE.
H & E Staining
(Low Magnification)

40). Human kidney (H & E)

Slide #1:
Human Kidney
Centrally located in this photo is the Renal Corpuscle and visible is the Bowman’s Capsule;
 Within the Bowman’s Capsule is the podocyte
 To the left side of the podocyte is the Distal Tubule (w/only a fragment of the
Afferent Arteriole visible)
 To the right side of the podocyte is the Proximal Convoluted Tubule
 Surrounding the podocyte is the Macula Densa
H & E Staining
(Medium Magnification)
Slide #1:
Human Kidney
Centrally located in this photo is visible the Bowman’s Capsule;
 The pointer is pointing to the Distal Tubule within the Podocyte
H & E Staining
(Medium Magnification)

41). Kidney (injection specimen to demonstrate the vascular bed)

Slide #2:
Human Kidney
Injection specimen to demonstrate the vascular bed
 This dye is a special type of liquid dye stain which forms into a gel
 Two structures can be seen: 1). Very large vein dyed blue
2). A renal corpuscle within which is visible the
Bowman’s capsule and Distal Tubule
H & E Staining
(Low Magnification)
Slide #2:
Human Kidney
Injection specimen to demonstrate the vascular bed
Visible in the centre of this image is the Glomerulosa
H & E Staining
(Medium Magnification)

42). Ureter (H & E)

Slide #4:
Human Ureter-transverse section
Visible in this image is transitional epithelium
H & E Staining
(Low Magnification)

Slide #4:
Human Ureter-transverse section
Visible in this image is transitional epithelium
H & E Staining
(Medium Magnification)

43). Urinary bladder (H & E)

Slide #6:
Human Urinary Bladder-cross section
Visible in this image are the smooth muscles in the urinary bladder
H & E Staining
(Low Magnification)

Slide #6:
Human Urinary Bladder-cross section
Visible in this image are from (right to left): Transitional epithelium with Lamina Propria
beneath it
Note: Distinguishing characteristic is the smooth muscle layer & transitional epithelium
KEY POINT: VACUOLES AROUND THE NUCLEUS!!!
H & E Staining
(Low Magnification)
Slide #6:
Human Urinary Bladder-cross section
Visible in this image are Nerve Bundles in the urinary bladder
H & E Staining
(Low Magnification)

44). Testis (H & E)

Slide #1:
Human Testis
H & E Staining
(High Magnification)
Slide #1:
Human Testis
Visible in this picture are: Border of seminiferous tubules, Fibroblasts within the border of
seminiferous tubules, Sertoli Cells (not round), and Gametogenic cells.
H & E Staining
(Low Magnification)

45). Epididymis and ductuli efferentes (H & E)

Slide #2:
Human Epididymis
 Visible in this picture is the Pseudostratified Epithelium of the Epididymis
 These are the Ductuli Efferentes (No “cilia”), i.e., the head
H & E Staining
(High Magnification)

46). Spermatic cord with ductus deferens (H & E)

Slide #3:
Human Epididymis
Visible in this picture is the Ductus Deferens;
 Visible prominently are the concentric circular smooth muscle layer (interior) and
longitudinal smooth muscle layer (exterior)
H & E Staining
(Low Magnification)

47). Prostate gland (H & E)

Slide #3:
Human Prostate Gland
Visible in this picture is the fibromuscular stroma (pink) and the secretory elements (parenkyma)
which are purplish colour;
H & E Staining
(Low Magnification)

48). Ovary (H & E)

Slide #5:
Ovary
Visible in this slide are (from top left of slide down to bottom right): Primordial follicles next to 1o
follicles. This layer constitutes the germinative epithelium. The large follicle seen in the centre of
the slide is a 2o Follicle, which is internally surrounded by purple stained by Granulosa Cells.
Right beneath it are visible a couple of Atretic ova.
H & E Staining
(Medium Magnification)
Slide #5:
Ovary
Visible centrally in this slide is: A 2o follicle
H & E Staining
(Medium Magnification)
Slide #5:
Ovary
Visible in this slide is: A Graafian follicle
Personal Note: The follicle is so massive that it takes up the entire field of view, and more, of the
slide!
H & E Staining
(Medium Magnification)
Slide #5 (2nd one):
Ovary
Visible in this slide is: One massive Corpus Luteum is seen during pregnancy in women in their
ovaries.
H & E Staining
(Medium Magnification)

49). Oviduct (H & E)

Slide #6:
Oviduct
Visible in this slide is (from top right to bottom left): Connective tissue and smooth muscle layer.
The finger-like projections are simple ciliated columnar epithelium, and a covering layer of cilia.
H & E Staining
(Medium Magnification)
Slide #6:
Oviduct
Visible in this slide is: The finger-like projections are simple ciliated columnar epithelium, and a
covering layer of cilia which is easily visible.
H & E Staining
(High Magnification)

50). Uterus (H & E)

Slide #6:
Uterus
Visible in this slide is (from top right to bottom left): The exterior perimetrium, the middle layer of
myometrium (composed of a muscle layer of circular, spiral, and longitudinal fibers), and the
internal endometrium layer (lined with mucosa, lamina propria, and a lining epithelium composed
of simple columnar epithelium).
H & E Staining
(Low Magnification)
Uterus (mouse)

51). Spinal cord (Stained with osmium and eosin or H & E)

Slide #3
Spinal Cord
Visible: gray matter, white matter, anterior roots, posterior roots, motor nerve cells
 The dark stained regions are the grey matter (to the right of the field pf view), and the
dark visible nuclei are the motor neurons in the ANTERIOR ROOTS
Stained w/Osmic Acid and Eosin
Slide #3
Spinal Cord
Visible in this picture is the Spinal Canal
Stained w/Osmic Acid and Eosin

Slide #3
Spinal Cord
Note: Posterior region. The neurons are much smaller and much less present
Stained w/Osmic Acid and Eosin

52). Cerebellum (Silver impregnation)

Slide #2:
Cerebellum
Stained with Silver Impregnation

52). b. Cerebellum (H & E)

Slide #2:
Cerebellum
Visible is the Medullary Core (nerve fibers);
 Visible in the darker, centre region is the Granular layer, with the Purkinje cell layer at
the periphery of this layer. The lighter pink stained region is the White Matter.
 Note: The Purkinje Cells are right at the border of the Granular layer
H & E Staining