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In some species, male and females appear very different, but in others the differences are subtle. In any case, the differences, both physiological and behavioral, are usually more apparent during mating season. 1. Genitalia
Behavior
o Males and females behave similarly, but in most species only the
males sing. They do this during mating season to attract the females. If you hear the frog singing or notice it has a vocal sack, it is most likely a male. In some cases, both males and females sing, but their song is different. Also, only male frogs try to mount other frogs, even if the frog they are mounting is not female. Male frogs will often mount other things such as logs, rocks and even people's shoes, mistaking them for female frogs. If you see a frog behaving this way, it is definitely male. Mating
o All male and female frogs have different genitalia and internal
sexual organs that allow them to breed. The sex organs on a frog are internal, however, so it is often difficult to tell the difference between males and females just by looking at them. Males have two internal testicles and a spermatic canal in which they produce sperm to fertilize the eggs. Female frogs have ovaries, oviducts and uteri. Both males and females have a cloaca, an orifice through which digestive waste and reproductive products, such as eggs and sperm, exit the body. The cloaca is usually more visible in females. Physical Features
o The most obvious difference between male and female frogs is the
role they play in mating. During mating, the female is submissive while the male jumps on top of her as she lays her eggs. The male fertilizes the eggs as they are released. Neither males nor females watch over the eggs or take care of the young, who will soon hatch into tadpoles and fend for themselves. Differences Among Species