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Foodborne Bacteria www.neogen.com/foodsafetyorder.htm

NEO-GRID™ / ISO-GRID™
Direct Coliform/E. coli Enumeration

Durable Results
ISO-GRID
Disposable
System
NEO-GRID
System

Coliforms E. coli under long

wave ultraviolet light

LMG Agar
Tryptose 10 g/L
Proteose Peptone SM27 5 g/L
Intended Use Yeast Extract 3 g/L
Lactose 12.5 g/L
Using Lactose Monensin Glucuronate (LMG) Agar, the ISO-GRID and
NEO-GRID Membrane Filter Systems are used to provide a method Monensin 0.038 g/L
for detecting and confirming a sample’s coliform and E. coli count. T7 0.15 g/L
Buffered MUG Agar (BMA) is used for E. coli confirmation. Aniline Blue (CI #42755) 0.1 g/L
Glucuronic Acid, sodium salt 0.5 g/L
The Test Agar 15 g/L
The ISO-GRID/NEO-GRID systems are based on the principle of Final pH: 7.2 ± 0.2
hydrophobic grid membrane filtration. Coliforms and E. coli are de-
tected through the use of a unique membrane filter containing a grid Buffered MUG Agar (BMA)
of 1,600 squares. First, a diluted sample is prefiltered to eliminate any Dibasic Sodium Phosphate 8.23 g/L
food particles present. The sample is then filtered through a hydrophobic Monobasic Sodium Phosphate 1.20 g/L
membrane, and the membrane is placed on LMG Agar. LMG Agar is Sodium Chloride 5 g/L
specifically formulated to detect coliforms. 4-Methylumbelliferyl-B-D-glucuronide 0.1 g/L (MUG)
After incubation, the membrane filter is examined for the presence Agar 15 g/L
of blue colonies. Any blue (presumptive coliform) colonies present Final pH: 7.2 ± 0.2
are confirmed using procedures detailed in the Methods Manual. To
detect E. coli, transfer the membrane filter to BMA and incubate. E. coli Ordering Information
colonies will fluoresce under long wave ultraviolet light. Prod.# Product description
Test Procedure For Use With Either the NEO-GRID or ISO-GRID Test System
6902A LMG Agar
1. Prepare a sample homogenate. 6903A BMA Agar
2. Filter 1.0 mL of homogenate through an ISO-GRID or 6810 1-Place Filtration Manifold
NEO-GRID hydrophobic grid membrane filter. 6800 3-Place Filtration Manifold
3. Place the membrane filter on Lactose Monensin Glucuronate 6801 6-Place Filtration Manifold
(LMG) Agar. 6821 Rubber Tubing (10 feet)
4. Incubate for 24 ± 2 hours at 35–37°C. Obtain coliform count. 6822 Side-Arm Flask Assembly (1 L)
5. Transfer membrane filter to BMA. 6824 Vacuum Pump - ¼ hp (Gast)
6. Incubate BMA plates for 2 hours at 35–37°C. 6828 Forceps
7. Examine filter under long wave ultraviolet light. E. coli colonies For Use With The NEO-GRID Test System Only
flouresce bright blue-white on BMA. 6848 NEO-GRID Test System - Contains 48 individually
Refer to the Methods Manual for complete instructions, and other packaged membrane filtration systems
available test methods. 6825 NEO-GRID Silicone Stopper
6826 Filta Tips (box of 100)
Product Specifications 6827 Filter Bags - 24 oz Whirl-Pak (box of 250)
Approvals: AOAC Official Method #990.11 6829 1.0 mL Sterile Serological Pipettes (bag of 50)
For Use With The ISO-GRID Test System Only
Please see page 95 for the required ISO-GRID equipment and
accessories.

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