Manual de Servicio de Agilent

Maintaining Your Agilent LC and LC/MS Systems
Our measure is your success.
Agilent Systems for UHPLC and HPLC.............1 Pump Maintenance..........................................9

Pump Maintenance Tips....................................................................10 Maintenance Procedures ..................................................................11 Ordering Information .........................................................................19
Autosampler Maintenance ............................27

Autosampler Maintenance Schedule ................................................28 Maintenance Procedures ..................................................................28 Ordering Information .........................................................................33
Fraction Collector Maintenance ....................39

Fraction Collector Maintenance Schedule ........................................40 Maintenance Procedures ..................................................................41 Ordering Information .........................................................................42
Valve Maintenance ........................................45

Valve Maintenance Notes .................................................................46 Ordering Information .........................................................................46
Detector Maintenance...................................53
Detector Maintenance Tips ...............................................................54 Maintenance Procedures ..................................................................55 Ordering Information .........................................................................59
LC/MS System Maintenance ........................69

LC/MS Maintenance Schedule .........................................................70 Ordering Information .........................................................................71
General Supplies............................................79
Ordering Information .........................................................................80
Welcome to the next generation of HPLC

Agilent's UHPLC and HPLC Systems are designed for the ultimate robustness, reliability, and performance.
No matter what your application may be, the bottom line is results. And Agilent's UHPLC and HPLC platforms, combined with ZORBAX Rapid Resolution High Definition (RRHD) and ZORBAX Rapid Resolution High Throughput (RRHT) columns, help you achieve the best results. This maintenance guide will help you keep your Agilent LC systems running at optimal performance and peak efficiency. Inside this guide you'll find:
Essential facts about Agilent supplies and accessories Vital maintenance procedures and troubleshooting tips All the Agilent part numbers you need in one easy-to-use guide
Order online at www.agilent.com/chem/store
Agilent Systems for UHPLC and HPLC
Agilent 1290 Infinity LC System

An infinitely better UHPLC system that meets all your LC and LC/MS needs
The Agilent 1290 Infinity LC is designed to provide highest speed, resolution and sensitivity. A new power range allows you to deploy any particle type, any column dimensions, or any mobile and stationary phases. Innovative technology components offer the next level of performance for both UHPLC and HPLC applications. The 1290 Infinity LC is the first system to provide the foundation for method transfer to or from any Agilent or non-Agilent UHPLC or HPLC system. In short, it gives you infinite capabilities to solve all your analytical challenges in LC and LC/MS.
ZORBAX Rapid Resolution High Definition (RRHD) 1.8 m Columns

Achieve stability up to 1200 bar for use with the Agilent 1290 Infinity LC. Available in 50, 100 and 150 mm lengths for fast or high resolution separations of your most complex samples.
For more information, visit www.agilent.com/chem/RRHD
Widest power range for infinitely better performance and flexibility
Infinite power range of the Agilent 1290 Infinity LC combines ultra high pressure up to
1200 bar and high flow rates up to 5 mL/min for maximum chromatographic performance, compatibility, flexibility and investment protection New 1290 Infinity Binary Pump combines active damping combined with lowest delay volumes facilitated by proprietary multi-layer technology in the new Agilent Jet Weaver Mixer to provide ultra-fast gradients and superior LC/UV and LC/MS performance New optical design in 1290 Infinity Diode Array Detector including Agilent Max-Light Cartridge Flow Cell offers a new level of UV-sensitivity and baseline robustness, including fast spectral acquisition at data rates up to 160 Hz Next generation flow-through design of the 1290 Infinity Autosampler achieves highest precision for both large and small injection volumes without changing sample loops New 1290 Infinity Flexible Cube combines with Autosampler to facilitate fixed-loop injection mode for ultra-fast cycle times, or automatic backflushing of needle seat for a new benchmark in lowest carryover New Agilent Quick-Change Valves in the 1290 Infinity Thermostatted Column Compartment boosts usability and paves the way for ultra high-throughput, multi-method and automated method development solutions
The power range of the Agilent 1290 Infinity LC is the widest available, combining ultra high pressures up to 1200 bar and high flow rates up to 5 mL/min for maximum chromatographic performance, compatibility, flexibility and investment protection.
Agilent 1290 Infinity LC System

Description 1290 Infinity Binary Pump 1290 Infinity Autosampler 1290 Infinity Flexible Cube 1290 Infinity Thermostatted Column Compartment 1290 Infinity Diode Array Detector Part No. G4220A G4226A G4227A G1316C G4212A
For more information, visit www.agilent.com/chem/1290
Agilent 1200 Series Rapid Resolution LC System

Discover the plus in performance
Increased productivity, confidence in analytical results and cost-effectiveness are key objectives in todays analytical laboratories. The Agilent 1200 Series Rapid Resolution LC (RRLC) System is designed to meet these challenges by delivering significantly faster results with higher data quality. On one system you can push RRLC performance to new limits and continue to run your conventional methods, too. Discover now the plus in separation power and detection capabilities with new innovative system components, providing you with a new level of sample information for more informed decisions in shorter time. Furthermore, the Agilent 1200 Series Rapid Resolution LC System offers new levels of serviceability, uptime and compliance.
Get the maximum in speed, resolution, sensitivity and flexibility
High resolution chromatography 90,000 plates in 4 minutes Full compatibility with existing HPLC methods More detection capabilities from UV-Visible and ELSD through LC/MS Near-zero sample carryover for uncompromised data quality High system flexibility for automated method development
Agilent 1200 Series Rapid Resolution LC System

Description 1200 Series Micro Degasser 1200 Series Binary Pump SL 1200 Series High Performance Autosampler SL Plus 1200 Series Standard Autosampler SL 1200 Series Thermostat for Autosamplers (2x) 1200 Series Sample Capacity Extension CTC Analytics HTC PAL Injection System CTC Analytics HTS PAL Injection System 1200 Series Thermostatted Column Compartment SL 1200 Series Variable Wavelength Detector SL Plus 1200 Series Multiple Wavelength Detector SL 1200 Series Diode Array Detector SL 1200 Series Fluorescence Detector 1200 Series Evaporative Light Scattering Detector Part No. G1379B G1312B G1367D G1329B G1330B G2257A G4270-CTC G4271-CTC G1316B G1314E G1365C G1315C G1321A G4218A
Agilent ZORBAX Rapid Resolution High Throughput Columns

Maximize your performance by combining the Agilent 1200 Series Rapid Resolution System with Agilent ZORBAX Rapid Resolution HT columns. ZORBAX RRHT columns use a totally porous, 1.8 m particle to provide maximum resolution in fast, ultra-fast and high resolution analyses.
For more information, visit www.agilent.com/chem/rrht
Agilent 1200 Series Standard LC Systems

The leading industry-standard for quality, reliability and robustness
Whatever your liquid chromatography analysis needs are, you will find your individual solution within the Agilent LC portfolio. The Agilent 1200 Series is the industry-leading standard, offering everything you need for analytical HPLC methodologies. From routine analysis through new product research and development in chemical analysis and pharmaceutical industries, the Agilent 1200 Series helps you achieve reliable and robust results for all your applications. Make a secure investment in the future of your lab with the scalable and open architecture of the Agilent 1200 Series HPLC solutions.
1200 Series Isocratic LC System

The LC workhorse for demanding QA/QC applications
Entry-level system for world-class LC technology Easy and fast maintenance Flow rates up to 10 mL/min for a wide range of column dimensions and applications Upgrade options from isocratic to automated quaternary LC system
1200 Series Quaternary LC System

For routine method development and high-throughput gradient applications
Greatest flexibility for multi-solvent gradients by accessing up to 4 solvents Flow rates up to 10 mL/min support narrow bore, standard and
semi-preparative applications Wide selection of autosamplers for full flexibility in injection volumes and sample containers
1200 Series Binary LC System

For research, high-throughput and fast applications
High pressure gradient mixing for superior gradient performance even at lower flow rates Optimized delay volumes for fast chromatography Flow rates from 0.05 to 5 mL/min provide ideal support for narrow and standard
bore applications
Agilent 1200 Series Standard LC Systems

Description 1200 Series Vacuum Degasser 1200 Series Micro Degasser 1200 Series Isocratic Pump 1200 Series Binary Pump 1200 Series Quaternary Pump 1200 Series Manual Injector 1200 Series High Performance Autosampler 1200 Series Standard Autosampler 1200 Series Thermostat for Autosamplers (2x) 1200 Series Thermostatted Column Compartment 1200 Series Variable Wavelength Detector 1200 Series Multiple Wavelength Detector 1200 Series Diode Array Detector 1200 Series Fluorescence Detector 1200 Series Refractive Index Detector 1200 Series Evaporative Light Scattering Detector Part No. G1322A G1379B G1310A G1312A G1354A G1328B G1367B G1329A G1330B G1316A G1314B G1365D G1315D G1321A G1362A G4218A
Related Literature
Order the Agilent HPLC Column Selection Guide from your local Agilent Representative or Agilent Authorized Distributor using publication number 5990-4435EN.
Agilent Rack for LC Systems

Agilents NEW LC system rack eliminates clutter and keeps instruments secure
This sturdy steel rack gives you fast, safe access to instruments and cables during maintenance procedures and module transfers. It is suitable for all Agilent LC module stacks, and features:
Easy assembly that saves you time and trouble (see diagram below) Adjustable shelves that let you tailor the rack to suit your needs Open design that ensures proper airflow management and power distribution
of equipment and cabling
Agilent rack for LC system, 5001-3726
For more information, contact your local Agilent Representative or Agilent Authorized Distributor.
Related Literature
Order the complete 1200 Series brochure from your local Agilent Representative or Agilent Authorized Distributor using publication number 5989-5200EN.
Pump Maintenance
Regular pump maintenance will help you lower your operating costs and generate precise results that make you feel confident.
You can count on Agilent isocratic, binary, quaternary, capillary, and preparative pumps for superior flow and composition stability. And by following a regular maintenance routine, you can also count on maximum uptime and a steady, accurate solvent flow for the life of the pump. On the following pages, you will find step-by-step instructions for these procedures:
Exchanging the purge valve frit Replacing the active inlet valve cartridge Cleaning the solvent filter Exchanging the outlet ball valve Changing the pump seals Changing and inspecting the sapphire pistons Seal wear-in procedure Replacing the wash seals
You may perform these procedures all at once, or as needed. Keep in mind that some parts may need replacing more often than others, depending on your application and solvent preparation procedures.
Pump Maintenance
Pump Maintenance Tips

Symptom Solvent dripping out of waste outlet when valve closed Pressure ripple unstable Pressure ripple unstable Gradient performance problems, intermittent pressure fluctuations A pressure drop of > 10 bar across the frit (5 mL/min H2O with purge valve open) indicates blockage Leaks at lower pump head side Unstable retention time Pressure ripple unstable Seal life time shorter than normally expected Loss of wash solvent Cause Leak on pump head Solution Exchange the purge valve frit or the purge valve
Dirty active inlet valve cartridge Run leak test for verification and exchange the active inlet valve cartridge Leak on pump head Solvent filter is blocked Dirty frit High seal wear Run leak test for verification and exchange the outlet ball valve sieve or the complete valve Change the solvent filter Exchange the purge valve frit or the purge valve Run leak test for verification and exchange the pump seals Check plungers while changing the seals Exchange the wash seals
Scratch on plunger Leaky wash seals
10
Pump Maintenance
Tools required
Maintenance Procedures
Exchanging the Purge Valve Frit
Maintenance Procedure
1. Using a 1/4 inch wrench, disconnect the pump outlet capillary at the purge valve. 2. Disconnect the waste tube. Beware of leaking solvents due to hydrostatic pressure. 3. Using a 14 mm wrench, unscrew the purge valve and remove it from the purge valve holder. 4. Remove the plastic cap with the gold seal from the purge valve. 5. Use a pair of tweezers or a toothpick to remove the frit. 6. Place a new frit into the purge valve with the slit facing the gold seal. 7. Replace the cap with the gold seal. Note: Before reinstallation, always check the gold seal. A deformed seal or a cracked cap should be replaced. 8. Insert the purge valve into the purge valve holder and orient the waste outlet nozzle downward. 9. Tighten the purge valve and reconnect outlet capillary and waste tubing.
Wrench 1/4-5/16 inch Wrench 14 mm Wrench 1/4 inch Pair of tweezers

or toothpick
11
Pump Maintenance
Tools required
Replacing the Active Inlet Valve Cartridge

Preparations
1. Switch off pump at the main power switch and unplug the power cable. 2. Unplug the active inlet valve cable from the connector. 3. Disconnect the solvent inlet tube at the inlet valve (beware of leaking solvents). Note: Binary pumps without the solvent selection valve (SSV) have an adapter installed between the solvent line and the active inlet valve (AIV). Disconnect the solvent tubes at the adapter and remove the adapter from the AIV.
Wrench 14 mm Pair of tweezers
Active inlet valve body
1. Using a 14 mm wrench, loosen the active inlet valve and remove the valve from pump head. 2. Use tweezers to remove the valve cartridge from the actuator assembly.
Valve cartridge
3. Clean the area in the actuator assembly. Flush the cartridge area thoroughly. 4. Insert a new valve cartridge into the actuator assembly (make sure the valve cartridge is fully inserted into the actuator assembly). 5. Insert the valve into the pump head. Using the 14 mm wrench, turn the nut until it is hand tight. 6. Position the valve so that the solvent inlet tube connection points towards the front. 7. Using the 14 mm wrench, tighten the nut by turning the valve in its final position (do not overtighten the valve). 8. Reconnect the active inlet valve cable to the connector and the inlet tube to the valve. Note: After an exchange of the valve cartridge, it may take several mL of pumping with the solvent used in the current application before the flow stabilizes at a low percentage-ripple as low as when the system was working properly.
Plug
Active inlet valve
Solvent inlet tube
12
Pump Maintenance
Cleaning the Solvent Filter

If the filter is in good condition, the solvent will freely drip out of the solvent tube (hydrostatic pressure). If the solvent filter is partially blocked, only very little solvent will drip out of the solvent tube. Caution: Small particles can permanently block the capillaries and valves of the module.
Always filter solvents Never use the module without solvent inlet filter
1. Remove the blocked solvent filter from the bottle head assembly and place it in a beaker with concentrated nitric acid (35%) for one hour. 2. Thoroughly flush the filter with bidistilled water (remove all nitric acid, some capillary columns can be damaged by nitric acid). 3. Replace the filter.
13
Pump Maintenance
Tools required
Exchanging the Outlet Ball Valve

Before exchanging the outlet ball valve, you can try to clean it in a sonic bath. Remove the gold seal and the sieve. Place the valve in upright position (onto the plastic cap) in a small beaker with alcohol. Place in a sonic bath for 5 to 10 minutes. Insert a new sieve and replace the gold seal.
Wrench 1/4 inch Wrench 14 mm Pair of tweezers
Valve body
1. Using a 1/4 inch wrench, disconnect the valve capillary from the outlet ball valve. 2. With a 14 mm wrench, loosen the valve and remove it from the pump body. 3. Remove the plastic cap with the gold seal. 4. Remove the sieve with a pair of tweezers. Note: Check the gold seal. It should be exchanged when badly deformed. Place the valve in an upright position, insert the sieve into the recess and replace the gold seal with the cap. Make sure that the sieve cannot move and is away from the gold seal area.
Sieve (G1312A/B only) Gold seal
5. Place a new sieve into the recess of the outlet ball valve and replace the cap with the gold seal.
Plastic cap
6. Reinstall the outlet ball valve and tighten the valve. 7. Reconnect the valve capillary.
Valve capillary
Outlet ball valve
14
Pump Maintenance
Pistons and Seals

Agilent pistons are made from a high purity, monocrystalline sapphire for maximum durability. Although ceramic pistons can be manufactured at a lower cost, ceramic is a sintered, polycrystalline material, which can cause undesirable variations during the production process. Agilent sapphire pistons are:
Meticulously cut at just the right angle, making them the most durable and longest
Sapphire piston and seals
lasting pistons in the world
Precisely aligned in their stainless steel holder to minimize wear on the piston and seal
Agilent seals are designed to fit snugly around our pistons, and are capable of adapting to a wide range of flow rates and pressures. Agilent piston seals are:
Spring-loaded and engineered to deliver optimal performance over highly dynamic flow
and pressure ranges
Manufactured from a proprietary polymer blend, and feature a spring made from the same
high-quality stainless steel that is used in our pump's flow path The combination of our piston and seal has undergone extensive testing under temperature stress, with all common HPLC solvents, and in many instruments. More importantly, they yield consistent, reproducible results.
15
Pump Maintenance
Changing the Pump Seals

Pump seals should be replaced when there are leaks on the bottom of the pump head, when retention times are inconsistent or when the pressure ripple is unstable.
All pumps except the G1361A preparative pump and the G4220A Infinity pump
1. Disassemble the pump head and use a piston to remove the two seals. 2. Clean the pump chambers and seal areas by flushing with solvent and wiping with a lint-free cloth. 3. Insert new seals. 4. Reassemble the pump head. 5. Perform the seal wear-in procedure (standard seals 5063-6589 only). Note: The wear-in procedure involves flushing the seals with isopropanol at high pressure while they are in place.
Changing and Inspecting the Sapphire Pistons

Each time you replace the seals, examine the pistons for scratches, which can cause small but damaging leaks.
All pumps except the G1361A preparative pump
1. Disassemble the pump head assembly. 2. Remove any deposits particularly buffers from the piston surface using alcohol or toothpaste. Replace the pistons if scratched. 3. Rinse thoroughly in alcohol and HPLC-grade water. 4. Reassemble the pump head. 5. Check to make sure there are no fractures in the springs. Replace piston housing assembly if springs are damaged. 6. Reinsert the pistons.
16
Pump Maintenance
Tools required
Seal Wear-In Procedure

Important: Do not perform this procedure when installing normal phase seals (0905-1420). It will cause severe damage to the seals.
Solvent bottle or beaker Isopropanol AIV adapter (0100-1847) Restriction capillary

(5022-2159)
Recommended after replacing standard seals only (5063-6589)
1. Fill the bottle with 100 mL of isopropanol, and place it in the solvent cabinet. Then, place the new pump head tubing (including bottle head assembly) into the bottle. 2. Screw the adapter to the active inlet valve. Connect the inlet tube from the bottle head directly to the adapter (quaternary pumps and binary pumps with selection valves only). 3. Connect the restriction capillary to the purge valve. Insert the other end of the capillary into a waste container. 4. Open the purge valve and purge the system for 5 minutes with isopropanol at a flow rate of 2 mL/min. 5. Close the purge valve and set the flow to a rate that is adequate to achieve a pressure of 350 bar. Pump for 15 minutes at this pressure to wear in the seals. You can monitor the pressure at your analog output signal using the handheld controller, ChemStation or any other controlling device connected to your pump. 6. Turn OFF the pump flow and slowly open the purge valve to release pressure from the system. 7. Disconnect the restriction capillary and reconnect the outlet capillary to the purge valve. Reconnect the appropriate tubing to the active inlet valve. 8. Rinse your system with the solvent that you plan to use for your next application.
17
Pump Maintenance
Tools required
Replacing the Wash Seals

1. Switch off binary pump at power switch. 2. Remove the seal keeper and the seal wash support rings from the plunger housing. Remove the seal keeper from the support ring assembly. 3. Using the blade of a flat head screwdriver, remove seal wash gasket and the secondary seal from the support ring. 4. Use the insert tool to press the seal (spring pointing upwards) into the recess of the support ring. Place a seal wash gasket in the recess of the support ring and replace the seal keeper. 5. Reassemble the pump head assembly.
Hexagonal key 3 mm Hexagonal key 4 mm Insert tool Small flat head

screwdriver
18
Pump Maintenance
Ordering Information
Purge Valve
Description Purge valve assembly Purge valve assembly, 400 bar Purge valve assembly, 600 bar
Purge valve assembly, G1311-60009
Comments For 1120 RRLC pump For 1100/1200 pumps Includes PTFE frit For 1200 RRLC pump Includes PTFE frit For 1290 purge valve For 1290 purge valve For 1120 RRLC pump For 1100/1120/1200 RRLC, 1120 and 1290 pumps For 1120 RRLC pump For 1100/1200/1200 RRLC, 1120 and 1290 pumps For 1100/1200/1200 RRLC and 1120 pumps
Part No. G4280-60031 G1311-60009 G1312-60023 5068-0005 5068-0004 G4280-60033 01018-22707 0905-1192 5001-3707 5062-2485
5 position/7 port rotor seal 5 position/7 port stator Purge valve actuator PTFE frits, 5/pk Seal for purge valve Gold seal, outlet Outlet caps, 4/pk
PTFE frits, 01018-22707
19
Pump Maintenance
Inlet Valves
Description
Active Inlet Valve (AIV)
Active inlet valve, G1312-60025
Comments For 1100/1200/1200 RRLC pumps
Part No. G1312-60025 5062-8562 G1312-60020 5001-3708
Active inlet valve, without cartridge
Cartridge for active inlet valve, 400 bar For 1100/1200 pumps Cartridge for active inlet valve, 600 bar For 1200 RRLC pump Gold seal for inlet valve
Passive Inlet Valve (PIV)
For 1100/1200/1200 RRLC and 1120 pumps For 1120 Compact LC System For 1290 Infinity LC For 1200 Prep Pump
Passive inlet valve Inlet valve

Valve
Cartridge, 400 bar, 5062-8562
G4280-60005 G4220-60022 G1361-60012
Valve assembly, inlet/outlet
Outlet Ball Valve

Description Outlet ball valve Comments For quaternary, isocratic 1100/1200 and 1120 pumps For binary pump, 400 bar
Outlet ball valve, G1311-60012
Part No. G1311-60012 G1312-60012 G1312-60022 G4220-60028 5001-3707 5062-2485 5063-6505
For binary SL pump, 600 bar For 1290 Infinity LC Gold seal, outlet Outlet caps, 4/pk Sieves for outlet ball valve, 10pk For 1100/1200/1200 RRLC and 1120 pumps For 1100/1200/1200 RRLC and 1120 pumps For G1312-60012 valve
20
Pump Maintenance
Solvent Reservoir and Supplies

Description
Solvent Reservoir
Part No. 9301-1420 9301-1421 9301-0656 9301-1450 5065-4421 9301-6341 9301-6342 G1311-60003 G1312-68716 G1376-60003 G1361-60022 G4220-60007 5063-6531
Solvent reservoir, 1 L Solvent reservoir, 1 L with cap Solvent reservoir, 1 L

Solvent reservoirs, 9301-1421
Solvent reservoir, amber, 1 L Solvent bottle, clear, 2 L, 2 inlets Solvent bottle, amber, 2 L Solvent bottle, clear, 2 L
Bottle Head Assembly
Bottle head assembly for screw bottle Bottle head assembly for F29/32 tapered solvent bottle Bottle head assembly with tubing and filter For capillary and nano systems (with stainless steel solvent filter) Bottle head assembly for prep system Bottle head assembly for 1290 Infinity LC System Bottle cap with 3-hole insert
21
Pump Maintenance
Solvent Filters
Description Glass filter, solvent inlet, 20 m pore size Glass filter, solvent inlet, 40 m pore size Glass filter, solvent inlet, 40 m pore size Stainless steel filter Recommended Use Analytical scale, micro scale Preparative LC For G2258A Dual Loop Autosampler For use in capillary and nano systems Part No. 5041-2168 3150-0944 3150-0944 01018-60025 Frit Adapter Frit adapter, PTFE, 3 mm, 4/pk Frit adapter, PTFE, 4 mm Frit adapter, PTFE, for 1/8 in. OD tubing Part No. 5062-8517 G1361-23204 G2258-23201
Pistons and Seals

Piston Description Comments Sapphire piston For 1100/1120/1200/1200 RRLC pumps Part No. 5063-6586 Seal Description Piston seals, graphite filled PTFE (reversed phase), 2/pk Piston seals, polyethylene (normal phase) Piston Sapphire plunger For 1290 Infinity LC For 1200 Prep Pump 5067-4603 G1361-22402 Pump seal PE for 1290 Infinity Binary Pump Piston seal for G1361A Preparative Pump Part No. 5063-6589 0905-1420 0905-1719 5022-2188
22
Pump Maintenance
Seal Wash
Description Peristaltic pump cassette with silicone tubing
Peristaltic pump, 5042-8507
Comments For 1100/1200/1200 RRLC pumps
Part No. 5042-8507 5065-9978
Silicone tubing, 1 mm ID, 3 mm OD, 5 m Peristalitic pump with ChemSure tubing ChemSure tubing for peristaltic pump
Wash Seal and Wash Keeper
For 1100/1200/1200 RRLC pumps
5065-9952 5042-8954
Wash seal Seal keeper Wash seal gasket, 6/pk Wash seal PE Support ring Backup ring for seal holder
Seal Wash Kit
For 1100/1200/1200 RRLC and 1120 pumps For 1100/1200/1200 RRLC and 1120 pumps For 1100/1200/1200 RRLC and 1120 pumps For 1290 Infinity Binary Pump For 1290 Infinity Binary Pump with seal wash For 1290 Infinity LC Pump
0905-1175 5001-3743 5062-2484 0905-1718 G4220-63010 G4220-24013
Active seal wash kit for isocratic or quaternary pumps Active seal wash kit for binary pumps
Includes 2 wash seal gaskets, 2 pump seals, peristaltic pump (includes pump cassette and motor), 2 seal keepers, 2 support ring assemblies, seal insert tool and silicone tubing Includes 4 wash seal gaskets, 4 pump seals, 2 peristaltic pumps (includes pump cassette and motor), 4 seal keepers, 4 support ring assemblies, seal insert tool and silicone tubing Includes 2 wash seal gaskets, 4 m flex tubing, 2 pump seals, 1 flow regulator, 2 seal keepers, 2 support ring assemblies, 20 mL luer lock syringe, seal insert tool and abrasive paper
G1311-68711
G1312-68711
Continuous seal wash kit
01018-68722
23
Pump Maintenance
Vacuum Degassers
Description Tubing kit, degasser to pump, 4/pk, 30 cm pieces of tubing with screws and bushings Mounting tool for flangeless nut
Plastic tubing cutter, 8710-1930
Comments For G1322A, G1379A/B For G1322A, G1379A/B For G1322A, G1379A/B For G1322A, G1379A/B For G1322A, G1379A/B For G1322A, G1379A/B For G1322A, G1379A/B For G1322A For G1322A For G1379A For G1379A For G1379B For G1322A, G1379A/B
Part No. G1322-67300 0100-1710 8710-1930 5063-6598 5063-6599 5022-2155 5062-2483 5062-8534 9301-1337 G1379-60010 G1379-67310 5042-8922 G1322-68705
Plastic tubing cutter Tefzel ferrules and SS lock rings, 1/8 in., 10/pk PPS nuts, 1/8 in., 1/4-28 thread, 10/pk Union, 1/4-28 thread, polypropylene PTFE solvent tubing, 5 m, 1.5 mm ID, 3 mm OD Disposable syringes, 20 mL, 10/pk Syringe adapter, 1/16 in. OD, 2 in. long Dual channel micro degasser vacuum chamber Micro vacuum degasser tubing kit for G1379A
Ferrules and rings, 5063-6598
Micro vacuum degasser tubing kit for G1379B Online degasser accessory kit Includes 8 screws, 8 bushings, 4 markers, tubing, syringe and syringe adapter
PPS nuts, 5063-6599
24
Pump Maintenance
Maintenance Kits
Description
Start-up Kits
Comments Includes 1 outlet cap, 5 PTFE frits, 4 piston seals, 1 outlet gold seal, 2 glass solvent inlet filters, 20 m, and 1 cartridge for active inlet valve Includes PEEK coated fused silica capillaries, column and fittings to start up a Nanoflow LC System Includes 2 wash seal gaskets, 2 pump seals, peristaltic pump (includes pump cassette and motor), 2 seal keepers, 2 support ring assemblies, seal insert tool and silicone tubing Includes 4 wash seal gaskets, 4 pump seals, 2 peristaltic pumps (includes pump cassette and motor), 4 seal keepers, 4 support ring assemblies, seal insert tool and silicone tubing Includes 2 wash seal gaskets, 4 m flex tubing, 2 pump seals, 1 flow regulator, 2 seal keepers, 2 support ring assemblies, 20 mL luer lock syringe, seal insert tool and abrasive paper Includes piston seal, PTFE frits, 2 gold seals and 2 outlet caps Includes 4 piston seals, PTFE frits, 3 gold seals, 2 sieves and 3 outlet caps Includes 5063-6589 piston seals (2), 01018-22707 PTFE frits (5/pk), 5062-8562 cartridge active inlet valve, G1311-60012 outlet ball valve and 5063-6586 pistons (2) Includes 4 pump seals, 4 stainless steel sieves, 4 gold seals, 1 stainless steel frit and 4 outlet caps Includes 3150-0942 filter, 5022-2192 filter assembly, 5065-4445 peristaltic pump, 0890-1764 tubing and 4 prep pump seals Includes 2 wash seals and 1 pack of 6 wash seal gaskets For 1120 manual injector systems Includes piston seals, PTFE frits, gold seals and rotor seal For 1120 automated injector systems Includes piston seals, PTFE frits, gold seals, rotor seal, needle and needle seat
Part No. G1311-68710 G2228-68700
Pump start-up kit For 1100/1200/1290 RRLC pumps Nanoflow LC start-up kit
Seal Wash Kits
Active seal wash kit for isocratic or quaternary pumps Active seal wash kit for binary pumps Continuous seal wash kit For 1100/1200/1290 RRLC pumps
Preventive Maintenance Kits
G1311-68711 G1312-68711 01018-68722
PM kit for isocratic/quaternary pump PM kit for binary pump Extended PM kit For 1100/1200/1290 RRLC pumps Capillary pump PM kit PM kit for prep pump PM kit for seal wash option PM kit for manual injector PM kit for automated injector
G1310-68730 G1312-68730 5065-4499 G1376-68710 G1361-68710 G1310-68731 G4280-68710 G4280-68730
25
Pump Maintenance
Maintenance Kits
Description
Accessory Kits
(Continued)
Comments Includes 3 wrenches, 5 PTFE frits, tubing, capillary and wrist strap Includes side cover with fixed rail, top and right cover for pump housing, plus 6 connecting capillaries. Allows automatic switching between different delay volumes to optimize the system for 2.1 mm ID or 4.6 mm ID columns. Includes purge valve and holder, hex keys 2.5, 3 mm, 2 wrenches 1/2 in. x 1/16 in., wrenches 1/4 in. x 5/16 in. and 14 mm, wrist strap, torque adaptor and stainless steel frit, 0.5 m Includes stainless steel connecting capillaries, solvent mixer, 2 L solvent bottle, bottle head assembly, filter, glass stop valve, stainless steel union, tubing and other parts Includes 8 screws, 8 bushings, 4 markers, tubing, syringe and syringe adapter Includes all parts to go from 20 L/min to 100 L/min flow rate in a capillary LC system Includes position sensing, 10 mL loop, 25 mL syringe, ring mounting bracket, start cable and SS connecting capillaries, 0.5 mm ID, 40 cm and 60 cm Part No. G1311-68705 G1312-68726
1100/1200 pump accessory kit Pump configuration kit for G1312B Needs G1158B 2 position/6 port valve Capillary pump accessory kit
G1376-68705
Accessory kit prep pump/gradient G1361A Online degasser accessory kit Extended flow range kit, 100 L/min Manual prep injection valve kit, stainless steel
G1361-68707 G1322-68705 G1376-68707 5065-9922
26
Autosampler Maintenance
Ensuring peak performance, productivity, and peace-of-mind.
Your Agilent autosampler is designed to deliver accurate measurements, precise injection volumes, and high-quality data. And by following a regular schedule of preventive maintenance, you can ensure a lifetime of defensible results. This section will give you step-by-step instructions on how to perform crucial procedures such as:
Exchanging the needle Replacing the seat assembly Changing the rotor seal
You will also find the high-quality Agilent supplies you need to keep your autosampler running at top efficiency.
27
Autosampler Maintenance Schedule

Procedure Exchanging the needle assembly Exchanging the seat assembly Exchanging the metering seal Typical Procedure When needle shows indication of damage or blockage When the seat shows indication of damage or blockage When autosampler reproducibility indicates seal wear Time Required 15 minutes 10 minutes 30 minutes
Tools required
Exchanging the Needle
Warning: To avoid personal injury, keep fingers away from the needle area during autosampler operation.
Wrench 1/4 inch

(supplied in accessory kit) Hexagonal key 2.5 mm (supplied in accessory kit) Pair of pliers
Preparations
1. Select "Start" in the maintenance function "Change Needle." 2. When the needle is positioned approximately 15 mm above the needle seat, remove the front cover.
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1. Select "Needle Down" until the needle screw is aligned with the hole in the safety cover.
2. Remove the sample loop fitting from the needle fitting.
3. Loosen the fixing screw and lift out the needle.
4. Insert the new needle. Align the needle in the seat, then tighten the screw firmly.
5. Reconnect the sample loop fitting to the needle fitting.
6. Use "Needle Up" to lift the needle to a position approximately 2 mm above the seat.
7. Ensure the needle is aligned with the seat. 8. On completion of this procedure, install the front cover. 9. Select "End" in the maintenance function "Change Needle."
29
Tools required
Exchanging the Seat Assembly

Preparations
1. Select "Start" in the maintenance function "Change Needle." 2. Remove the front cover. 3. Use the "Needle Up" command in the "Change Needle" function to lift the needle an additional 1 cm.
Wrench 1/4 inch

(supplied in accessory kit) Flat head screwdriver
1. Disconnect the seat capillary fitting from the injection valve (port 5).
2. Use a small flat head screwdriver to ease out the needle seat.
3. Insert the new needle seat assembly. Press the seat firmly into position.
4. Connect the seat capillary fitting to port 5 of the injection valve.
5. Use "Down" to position the needle approximately 2 mm above the seat.
6. Ensure the needle is aligned with the seat. If required, bend the needle slightly until the needle is aligned correctly. 7. On completion of this procedure, install the front cover. 8. "Select "End" in the maintenance function "Change Needle."
30
Tools required
Replacing the Rotor Seal

Caution: The stator face is held in place by the stator head. When you remove the stator head, the stator face can fall out of the valve. Carefully handle the valve to prevent damage to the stator face.
Wrench 1/4 inch

(supplied in accessory kit) Hex key 9/64 inch (supplied in accessory kit)
1. Remove all capillary fittings from the injection valve ports.
2. Loosen each fixing bolt two turns at a time. Remove the bolts from the head.
3. Remove the stator head, stator face and stator ring.
4. Remove the rotor seal and isolation seal.
5. Install the new rotor seal and isolation seal. Ensure the metal spring inside the isolation seal faces towards the valve body.
6. Install the stator ring with the shorter of the two pins facing towards you at the 12 o'clock position. Ensure the ring sits flat on the valve body.
(Continued)
31
7. Place the stator face in place on the stator head.
8. Install stator head and stator face. Tighten the bolts alternating two turns at a time until the stator head is secure.
9. Reconnect the pump capillaries to the valve ports.
10. Slide the waste tube into the waste holder in the leak tray. 11. On completion of this procedure, install the front cover.
32
Injection Valves
Valve 2 position/6 port injection valve Use With G1313A, G1329A, G1367A, 1120 G1377A, G1389A Part No. Rotor Seal Material Tefzel PEEK 0101-1050 Vespel Rotor Seal Part No. 0100-1853 0100-1849 0100-2231 0100-2088 0101-1416 0100-2089 0101-1417 Stator Part No. Stator Face Part No. RheBuild Kit Part No. 0101-1257 0101-0921 Vespel 0100-1850 0100-1851
2 position/6 port micro injection valve 2 position/6 port injection valve, 600 bar 10 port, dual loop valve 2 position/6 port MBB injection valve 2 position/6 port ultra high pressure valve, 1200 bar
*Includes seal and stator face
G1367C SL, 0101-1422 PEEK G1367D SL Plus, G1329B G2258A G2260A G4226A 0101-1385 Vespel 0101-1267 PEEK 5067-4117 Vespel
0101-2415 0101-1268* 5068-0007
0101-1390 0100-2195 5068-0006 0101-1268
33
Needles and Needle Seats

Agilent Autosampler G1313A, G1329A, G1120A (automated sampler only) Needle Assembly Description Needle assembly, standard autosampler Part No. G1313-87201 Compatible with Needle Seat Standard needle seat 0.17 mm ID capillary, 2.3 L Standard needle seat 0.12 mm ID capillary, 1.2 L G1313A, G1329A, G1120A (automated sampler only) G1313A, G1329A, G1120A (automated sampler only) G1389A Needle assembly, for use with PEEK seat Needle assembly, 900 L upgrade Needle assembly, micro LC autosampler G1313-87203 G1313-87202 G1329-80001 Standard needle seat, PEEK 0.17 mm ID capillary, 2.3 L Standard needle seat 0.17 mm ID capillary, 2.3 L Micro LC needle seat 100 m ID capillary, 1.2 L Micro LC needle seat 50 m ID capillary, 0.3 L G1367A/B Needle assembly, well plate autosampler (green) Needle assembly, well plate autosampler (new, blue) G1367C SL Needle assembly, well plate autosampler G1367-87200 G1367-87201 G1367-87201 Needle seat, well plate autosampler 0.17 mm ID capillary, 2.3 L Needle seat, well plate autosampler 0.12 mm ID capillary, 1.2 L Needle seat, 600 bar, without capillary Seat capillary, 0.17 x 100 mm, 0.8 mm OD Seat capillary, 0.12 x 100 mm, 0.8 mm OD G1367D SL Plus Needle assembly, well plate autosampler (black) G1367-87202 Part No. G1313-87101 G1313-87103 G1313-87102 G1313-87101 G1329-87101 G1329-87103 G1367-87101 G1367-87102 G1367-87104 G1367-87302 G1367-87303
Needle seat, 600 bar, with seat capillary, G1367-87017 0.17 mm ID x 100 mm, 0.8 mm OD Needle seat, 600 bar, with seat capillary, G1367-87012 0.12 mm ID x 100 mm, 0.8 mm OD
34
Needles and Needle Seats

Agilent Autosampler G1377A
(Continued)
Part No. G1377-87201 Compatible with Needle Seat Micro needle seat with seat capillary, 100 m Micro needle seat with seat capillary, 75 m Micro needle seat with seat capillary, 50 m Part No. G1377-87000 G1377-87001 G1377-87002 G2258-87102 G2260-87101
Needle Assembly Description Needle assembly, micro well plate sampler
G2258A G2260A G1313A, G1120A (automated sampler only) G1313A, G1329A, G1389A, G1120A (automated sampler only) G1377A G4226A
Needle assembly, dual loop autosampler Needle assembly, prep autosampler Seat adapter Finger caps, 15/pk
G2258-68710 G2260-87201 G1313-43204 5063-6506
Twin needle seat, dual loop autosampler Needle seat, prep autosampler 0.5 mm ID, 20 L
Tool for micro seal capillary mounting Needle assembly, 1290 Infinity Autosampler
G1377-44900 G4226-87201 Seat assembly, 0.12 mm, 1290 Infinity LC G4226-87012
35
Metering Device Supplies

Infrequently, the metering device seal and piston may need replacement if you see loss in injection volume precision or metering device leaking.
Piston Description Sapphire piston, 40 L Sapphire piston, 100 L Sapphire piston, 900 L Piston, 5 mL Use With G1367D, G1389A, G1377A, G4226A G1313A, G1329A, G1120A, G1367A/B/C G1313A, G1329A, 1120 G2258A Part No. 5064-8293 5063-6586 5062-8587 Seal Description Piston seal, 2 mm, for G1367D, G1389A, G1377A Piston seal for G4226A Piston seals, graphite filled PTFE (reversed phase), 2/pk Metering valve seal Part No. 5022-2175 0905-1717 5063-6589 0905-1294 0905-1599
G2258-60003 Piston seal
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Autosampler Trays
Description 100 position tray for 2 mL vials 100 position tray for 2 mL vials, thermostattable 40 position tray for 2 mL vials 15 position tray for 6 mL vials External vial tray for 17 vials (disposal position) Disposal tube for external vial tray Well plate tray, 2 well plates, 10 vials (supports 50 mm plates) Vial plate for 54 x 2 mL vials, 6/pk Vial plate for 15 x 6 mL vials 100 position tray for micro vials Tray for 27 Eppendorf safe lock tubes, 0.5/1.5/2 mL 8.5 in. well plate rack, 2/pk For 16 shallow well plates, 4 deep well plates (max 48 mm height) or 6 vial racks 10 in. well plate rack, 2/pk For 20 shallow well plates (max height 16 mm), not compatible with deep well plates 8.5 in. well plate rack extension Includes 3 racks for 3 x 16 shallow well plates, 2 x 4 deep well plates (max 48 mm height) or 3 x 6 vial racks 10 in. well plate rack extension Includes 3 racks for 3 x 20 shallow well plates (max height 16 mm), not compatible with deep well plates 205H rack, two 96 deep well plates 200 rack, 13 x 100 mm tubes (9 mL), 96 207 rack, 16 x 100 mm tubes (12 mL), 75 209 rack, 12 x 32 mm tubes (12 mL), 96 94A special holding 1100 tray For G2250A For G1367A/B/C/D, G4226A For G1367A/B/C/D For G2257A For G1367A/B/C/D, G2258A, G4226A Comments For G1313A, G1329A, G1120A Part No. G1313-44510 G1329-60011 G1313-44512 G1313-44513 G1313-60004 G1313-27302 G2258-60011 G2255-68700 5022-6539 G4226-60021 5022-6538 G2255-68709 G2255-68710 G2255-68720 G2255-68730 G2250-04504 G2250-04503 G2250-04502 G2250-04501 G2250-04500
Vial plate, G2255-68700
Tray, 5022-6538
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Autosampler Kits
Description PM kit for 1200 autosampler SL G1313/27A autosampler maintenance kit G1313/27A autosampler accessory kit Comments Includes PEEK rotor seal, needle seat, needle, 2 seals and 15 finger caps Includes 3 hex keys, 2 wrenches, tubing, 1 label halftray, wrist strap, 15 finger caps, 0.17 ID x 180 mm capillary, 100 screw top 2 mL vials and caps, tray for 40-2 mL vials and tray for 15-6 mL vials Includes 1 each: rotor seal, isolation seal, stator face, needle and needle seat Includes Vespel rotor seal, standard needle seat and needle Includes opaque front and side doors and front cover Includes transparent front and side doors Includes 100/pk 2 mL screw top vials and caps, 10/pk 0.5 mL 96 well plates, tools, 380 mm 0.17 mm capillary and CAN cable Includes 100/pk 2 mL screw top vials and caps, 10/pk 0.5 mL 96 well plates, tools, 40 L loop, 50 cm-50 m fused silica/PEEK capillary, 75 m seat capillary and CAN cable Part No. G1329-68719 G1313-68705 Includes 1 rotor seal (Vespel), 1 needle, 1 needle seat, 2 metering seals and 15 finger caps G1313-68709
PM kit for standard autosampler PM kit for standard autosampler Light protection kit for G1329A Door replacement kit for G1329A Well plate sampler accessory kit PM kit for well plate autosamplers Micro well plate sampler accessory kit
5065-4498 G1313-68730 G1329-68718 G1329-68727 G1367-68705
Includes Vespel rotor seal, needle, needle seat, peristaltic pump cartridge and seal tight nut G1367-68730 G1377-68705 G2260-68705 G1313-68711 G4280-68710 G4280-68730
Accessory kit for G2260A prep autosampler Includes stainless steel connecting capillaries, hex keys, wrenches, 100/pk 2 mL screw cap vials and caps, tray for 6 mL vials, stainless steel union and other parts Multidraw upgrade kit for G1313A/G1327A/G1329A autosamplers PM kit, manual injector PM kit, auto injector Includes 500 L capillary, 1500 L capillary and ZDV union For 1120 manual injector systems Includes piston seals, PTFE frits, gold seals and rotor seal For 1120 automated injector systems Includes piston seals, PTFE frits, gold seals, rotor seal, needle and needle seat
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Fraction Collector Maintenance

A higher level of purity for results that stand up to scrutiny.
When you are under pressure to generate better results faster, there is no time to compromise on recovery and purity. And with Agilents 1100 and 1200 Series Fraction Collectors, you will not have to. Agilent fraction collectors are designed to process data in real time for instantaneous and precise fraction collection while increasing throughput on your purification system. So you can be certain that you are getting the highest degree of recovery and purity for your fractions even with low flow rates. In this section, you will find essential details about our analytical scale (AS) and preparative scale (PS) fraction collectors, including:
Fraction collector maintenance, including simple repairs you can perform without
a service call
Guidelines for maintaining the micro fraction collector and spotter Agilent-engineered parts, such as trays, collecting tubes, plates, and needle assemblies MALDI spotting accessories
39
Fraction Collector Maintenance Schedule

Procedure When to Perform
Analytical and Preparative Fraction Collector Maintenance Replace the inlet/waste tubing Replace the valve-to-needle tubing Exchange the preparative needle assembly Exchange the analytical needle assembly Once per year or when you notice signs of damage or wear Once per year or when you notice signs of damage or wear When the needle shows signs of damage or blockage When the needle shows signs of damage or blockage or when using the short needle assembly with high test tubes (>45 mm) When the valve is leaking or not switching properly When the flow delay sensor no longer works When defective, leaky, blocked or contaminated
Exchange the diverter valve Exchange the internal tray Repair or exchange a funnel within the internal tray or funnel tray
Micro Fraction Collector/Spotter Maintenance Replace fraction collector capillary Exchange the capillary guiding assembly Exchange the internal tray Exchange the flap septum and waste tubing At least every six months or when worn, blocked or damaged When bent or damaged When the flow delay sensor no longer works properly At least every six months or when defective or contaminated
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Tubing Replacement
There are two types of tubing available to connect the valve with the needle: preparative scale PTFE tubing (0.8 mm ID) and analytical scale tubing (0.25 mm ID). Both types should be replaced when they become contaminated, worn or damaged.
1. Position the fraction collector's transport unit in the "Home" position. 2. Remove all installed trays from the tray base. 3. Position the transport unit in the "Change Parts" position. 4. Turn off the instrument.
Capillary Replacement
Agilent offers three types of connecting capillaries each with different internal diameters. We recommend replacing the capillaries and flap septum every six months or when contaminated, blocked, worn, or visibly damaged.
1. Place the transport unit of the micro fraction collector/spotter in the "Home" position. 2. Remove all installed trays from the tray base. 3. Place the transport unit in the "Change Parts" position. 4. Turn off the instrument. 5. Unscrew the micro fraction collector/spotter capillary from the flow cell of the detector. Consult your user's manual for detailed replacement instructions.
41
Fraction Collector Capillary Kits and Needles
Module G1364B G1364C Max Flow Rate 100 mL/min 1 mL/min 10 mL/min 10 mL/min 100 mL/min G1364D 4 L/min 4-30 L/min 30-100 L/min Tubing ID (mm) 0.8 mm ID 0.15 mm ID 0.25 mm ID 0.25 mm ID 0.8 mm ID 25 m ID 50 m ID 100 m ID Tubing Kit G1364-68711 G1364-68723 G1364-68712 G1364-68712 G1364-68711 G1364-87304 G1364-87305 G1364-87306 50 mm 50 mm 20 mm 20 mm Needle Length Needle Typical Use G1364-87201 Tubes (max 100 mm) G1367-87200 Tubes (max 48 mm), well plates, vials G1367-87200 G1364-87202 Funnel tray (tubes max 75 mm) G1364-87202 MALDI targets, well plates
Collecting Tubes and Trays

Tray Part No. G1364-84523 G1364-84524 G1364-84525 G1364-84516 G1364-84532 Hole Diameter (mm) 30 25 16 12 Funnel tray No. of Tubes 40 60 126 215 40 Tube Dimensions 30 x 48 mm 25 x 100 mm 16 x 48 mm 12 x 48 mm Any size Tube Part No. Unit 5042-6470 100/pk 5042-6459 100/pk 5022-6533 100/pk 5022-6534 100/pk
42
Well Plate Trays

Description Tray for 4 well plates, cooled Tray for 4 plates, adjustable, cooled Tray for 2 well plates, 10 funnels, cooled Tray for 2 well plates, 10 vials, 2 mL Part No. G1364-84521 G1364-84531 G1364-84522 G1367-60001
Well Plates
Description 96 well plates, 0.5 mL, polypropylene 96 well plates, 0.5 mL, polypropylene 96 deep well plates, 1 mL, polypropylene Closing mats for 96 well plates, silicone, pre-slit, fits 96 well plates 5042-1385 and 5042-1386 only 96 well plates, 150 L, conical, polypropylene
Collection plate, showing 96-position closing mat, 5042-1389
Unit 120/pk 10/pk 50/pk 50/pk 25/pk 30/pk 25/pk
Part No. 5042-1385 5042-1386 5042-6454 5042-1389 5042-8502 5042-1388 5188-5375 5065-4402
384 well plates, 90 L, polypropylene 384 well plates, max 45 L, skirted 96 deep well collection plates with glass inserts, caps, and septa, pre-assembled, 0.35 mL Glass inserts, 350 L Caps/septa for glass inserts Vial plate for 54 x 2 mL vials Tray for 27 Eppendorf safe lock tubes, 0.5/1.5/2 mL Vial plate for 15 x 6 mL vials Plate for 24 tubes with 18 mm diameter
1000/pk 1000/pk 6/pk
5188-5321 5188-5322 G2255-68700 5022-6538 5022-6539 5042-8544
Tray, 5022-6538
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G1364D Micro Fraction Collector Supplies

Description MALDI spotting adapter for G1364D Well plate adapter assembly for G1364C/D Flap septum, PEEK, for internal tray Fused silica/PEEK capillary, 25 m, 50 cm Fused silica/PEEK capillary, 50 m, 50 cm Fused silica/PEEK capillary, 100 m, 50 cm Waste tube, PTFE, 20 cm, 1.4 mm ID, 2.0 mm OD MALDI plate carrier Bruker MALDI plate carrier Bruker PAC MALDI plate carrier ABI MALDI plate carrier ABI Opti-TOF MALDI plate carrier Agilent MALDI plate carrier Micromass Target plate for AP-MALDI LC/MS Calibration plate Bruker Calibration plate ABI 192 Calibration plate ABI 10x10 & 20x20 Calibration plate Agilent Calibration plate Micromass On-line matrix kit for MALDI spotting Includes BCD board/cable, syringe, needles, adapters, connector and capillary Adapter, female to female 1/4-28 Adapter, male luer to female 1/4-28 Syringe, glass, 1 mL, 1/4-28 connector Micro T-connector, PEEK, swept vol 29 nL, with 1/32 in. ID fittings MALDI spotting tips, PTFE, 10/pk Part No. G1364-83205 G1364-60021 G1364-27107 G1364-87304 G1364-87305 G1364-87306 G1364-86711 5022-6541 5022-6546 5022-6542 5023-0238 5022-6543 5022-6544 G1972-60025 5023-0208 5023-0209 5023-0213 5023-0214 5023-0215 G1364-68706 5042-8517 5042-8518 5181-1541 5042-8519 G1364-81701
MALDI spotting adapter, G1364-83205
Well plate adapter assembly, G1364-60021
Maldi plate carrier Bruker, 5022-6541
Calibration plate Bruker, 5023-0208
44
Valve Maintenance
Engineered for consistent results and optimal system performance.
Agilent's industry-leading Manual Injection Valves are designed to ensure trouble-free operation with your HPLC System. Our valves also feature patented "Make-Before-Break" architecture that allows you to switch between LOAD and INJECT positions without interrupting the flow. So you can analyze more samples in less time. In this section, you will find the latest in LC injection valve technology, along with important maintenance information that will maximize reproducibility.
45
Valve Maintenance
Valve Maintenance Notes

Vespel is a polyimide with low wear and high chemical resistance. Vespel tolerates a pH
range of 0 to 10. More basic solutions dissolve Vespel, which damages the rotor seal.
PEEK offers a high chemical resistance and versatility and will tolerate the entire pH range
from 0 to 14.
Tefzel is recommended for use in applications where PEEK cannot be used, such as
methylene chloride or DMSO in higher concentrations.
pH Range Vespel PEEK Tefzel 0-7 7-10 10-14
Injection Valves
Valve 2 position/6 port injection valve Use With G1313A, G1329A, G1367A, 1120 G1377A, G1389A G1367C SL, G1367D SL Plus, G1329B G2258A G2260A G4226A Part No. 0101-0921 RheBuild Kit Part No. 0101-1257 Rotor Seal Material Vespel Tefzel PEEK 0101-1050 0101-1422 Vespel PEEK Rotor Seal Part No. 0100-1853 0100-1849 0100-2231 0100-2088 0101-1416 0100-2089 0101-1417 Stator Part No. Stator Face Part No. 0100-1850 0100-1851
2 position/6 port micro injection valve 2 position/6 port injection valve, 600 bar 10 port, dual loop valve 2 position/6 port MBB injection valve 2 position/6 port ultra high pressure valve, 1200 bar
*Includes seal and stator face
0101-1385 0101-1267 5067-4117 0101-1268
Vespel PEEK Vespel
0101-2415 0101-1268* 5068-0007
0101-1390 0100-2195 5068-0006
46
Valve Maintenance
External Switching Valve Replacement Parts

Description 12 position/13 port preparative solvent selection valve 6 position/14 port column selection valve (six column selector) 2 position/10 port valve dual-sided MBB 2 position/6 port switching valve Use With G1160A G1159A G1157A G1158A RheBuild Kit Part No. 0101-1288 0101-1290 0101-1359 0101-1289 0101-1358 (with PEEK rotor seal) Vespel Tefzel PEEK HP PEEK blend 0100-1855 0100-1854 0100-2233 0101-1409 0100-2087 0101-1361 0101-1361 0101-1417 0100-2089 0101-1363 0101-1410 Rotor Seal Material Rotor Seal Part No. Stator Part No. 0101-1365 0101-1364 0101-1362 0100-1850
2 position/6 port switching valve, 600 bar 2 position/6 port micro switching valve 2 position/10 port micro switching valve 6 position/7 port selection valve
G1158B G1162A G1163A G1156A
Internal Switching Valves

Description 2 position/6 port column switching valve head, 400 bar Use With Valve Head G1316A 0101-0920 Rotor Seal Material Tefzel Vespel PEEK 2 position/6 port ultra high pressure valve head, 1200 bar 2 position/6 port micro column switching valve head, 400 bar 8 position/9 port low pressure valve head, 400 bar 8 position/9 port high pressure switching valve head, 600 bar 8 position/9 port ultra high pressure valve head, 1200 bar 2 position/10 port ultra high pressure valve head, 1200 bar 2 position/10 port switching valve head, 400 bar 2 position/10 port micro column switching valve head, 600 bar G1316C G1316A G1316C G1316C G1316C G1316C G1316A G1316B 5067-4117 0101-1051 5067-4108 5067-4107 5067-4121 5067-4118 0101-1343 0101-1419 Rotor Seal Part No. 0100-1854 0100-1855 0100-2233 5068-0008 0100-2087 5067-4113 5067-4111 5068-0002 5068-0012 0101-1361 0101-1415 5068-0006 0100-2089 5067-4112 5067-4110 5068-0001 5068-0011 0101-1362 0101-1421 Stator Part No. 0100-1851
47
Valve Maintenance
Manual Injection Valves

Agilent provides the latest developments in LC injection technology from Rheodyne.
Continuous flow path with "Make-Before-Break" design Sample capacity Choice of stainless or PEEK flow path Easy access to fittings due to wide 30 port angles
Manual Injection Valves with Position Sensing Switches
Description 7125 Comments Part No. N/A Rotor Seal Material Vespel Tefzel PEEK 7725i, stainless steel 9725i, PEEK 3725i, stainless steel prep valve 3725i, PEEK prep valve 7010/7000/7040 Manual prep injection valve kit, stainless steel Includes position sensing, 10 mL loop, 25 mL syringe, ring mounting bracket, start cable and SS connecting capillaries, 0.5 mm ID, 40 cm and 60 cm Analytical scale Analytical scale Preparative scale Preparative scale 5063-6502 0101-1253 0101-1232 0101-1231 N/A 5065-9922 PEEK PEEK PEEK PEEK PEEK Rotor Seal Part No. 0101-0623 0101-0620 0101-1255 0101-1255 0101-1255 0101-1233 0101-1233 0101-1256 0100-1860 0100-1859 0101-1254 Stator Part No. Stator Face RheBuild Kit Part No. Part No. 0101-0624
2 position/6 port Does not include 20 L loop manual injection valve and needle port for 1120
5067-4104
Vespel
5067-4105
0100-1850
48
Valve Maintenance
Manual Injection Valve Replacement Parts

Description Isolation seal Ring stand mounting bracket PEEK adapter, 1/8 in. to 1/16 in. Position sensor switch for manual valves For 3725i Comments For Rheodyne Series 7725, 9725, 3725 Part No. 1535-4046 1400-3166 5067-1503 0490-1849
Manual Injection Valve Sample Loops

The right mix of injection valve sample loops are available for your application needs. Agilent offers factory-cut and finished loops of the highest quality.
Stainless steel loops are square cut, free of burrs, for a flush connection Flexible PEEK loops have a clean, straight cut for low dead volume connections
Volume 5 L ID (mm) 0.18 0.18 0.18 10 L 0.30 0.30 0.25 20 L 0.51 0.30 0.25 50 L 0.51 0.51 0.51 100 L 0.51 0.51 0.51 Material SS SS PEEK SS SS PEEK SS SS PEEK SS SS PEEK SS SS PEEK Use With 7125 and 7010 7725 9725 7125 and 7010 7725 9725 7125 and 7010 7725 9725 7125 and 7010 7725 9725 7125 and 7010 7725 9725 Part No. 1535-4860 0101-1248 0101-1241 0101-0376 0100-1923 0101-1240 0101-0377 0100-1922 0101-1239 0101-0378 0100-1924 0101-1238 0101-0379 0100-1921 0101-1242
49
Valve Maintenance
Volume 200 L
ID (mm) 0.76 0.76 0.51
Material SS SS PEEK SS SS PEEK SS SS PEEK SS SS PEEK PEEK SS SS PEEK PEEK PEEK PEEK
Use With 7125 and 7010 7725 9725 7125 and 7010 7725 9725 7125 and 7010 7725 9725 7125 and 7010 7725 9725 3725 7125 and 7010 7725 9725 3725 3725 3725
Part No. 0101-1252 0101-1247 0101-1237 0101-1251 0101-1246 0101-1236 0101-1219 0101-1245 0101-1235 0101-1250 0101-1244 0101-1234 0101-1229 0101-1249 0101-1243 0101-1230 0101-1228 0101-1227 0101-1226
500 L
0.76 0.76 0.76
1 mL
0.76 0.76 0.76
2 mL
1.00 1.00 0.76 1.6
5 mL
1.00 1.00 0.76 1.6
10 mL 20 mL
2.0 2.0
50
Valve Maintenance
Syringes for Manual Injection

New color-coded manual syringes let you identify critical volumes at a glance and help boost productivity along every step of your sample processing workflow
Syringes for manual injection
Agilent color-coded manual syringes allow you to determine syringe volume with one quick glance, so you can more efficiently perform manual dilution, extraction, and sample prep. They also give you the advantages of:
Improved scale readability with a new vertical syringe scale orientation Unique package design that allows you to store syringes right on your lab bench Accuracy within 1% of nominal volume, and with precision within 1%, measured at 80%
of total scale volume
Earth-friendly cardboard and recyclable plastic packaging Certificate of Conformance to ensure the highest quality construction and performance Lot traceable for accurate identification
LC Manual Syringes with Fitted Plungers
Volume (L) Description 5 10 Fixed Fixed Removable Replacement needle for 10 L syringe 25 50 100 250 500 Fixed Fixed Fixed Fixed Fixed 3/pk 22 gauge/2 in./LC tip 22 gauge/2 in./LC tip 22 gauge/2 in./LC tip 22 gauge/2 in./LC tip 22 gauge/2 in./LC tip Unit Needle Gauge/Length/Tip Part No. 22 gauge/2 in./LC tip 22 gauge/2 in./LC tip 22 gauge/2 in./LC tip 5190-1480 5190-1484 5190-1485 5190-1486 5190-1494 5190-1501 5190-1508 5190-1515 5190-1522
Related Literature
To select the best syringe for your application, order the Agilent Syringe Specifications and Selection Guide from your local Agilent Representative or Agilent Authorized Distributor using publication number 5990-3563EN.
51
Valve Maintenance
LC Manual Syringes with PTFE-Tipped Plungers

Volume (L) Description 10 Removable Replacement needle for 10 L syringe Replacement plunger with PTFE tip for 10 L syringe 25 Removable Replacement needle Replacement plunger with PTFE tip for 25 L syringe 50 Removable Replacement needle Replacement plunger with PTFE tip for 50 L syringe 100 Removable Replacement needle Replacement plunger with PTFE tip for 100 L syringe 250 500 Removable Replacement needle Removable Replacement needle Replacement plunger with PTFE tip for 500 L syringe 3/pk 3/pk 22 gauge/2 in./LC tip 22 gauge/2 in./LC tip 3/pk 22 gauge/2 in./LC tip 3/pk 22 gauge/2 in./LC tip 3/pk 22 gauge/2 in./LC tip 3/pk Unit Needle Gauge/Length/Tip Part No. 22 gauge/2 in./LC tip 5190-1492 5190-1486 5190-1558 5190-1499 5190-1571 5190-1560 5190-1505 5190-1571 5190-1561 5190-1512 5190-1571 5190-1562 5190-1520 5190-1571 5190-1526 5190-1571 5190-1564
52
Detector Maintenance
Ensure uncompromised chromatographic performance with these tips and techniques.
Agilent wavelength detectors combine exceptional flexibility with superior instrument control, data communication, and analytical capabilities. This section will show you how to maintain your detectors high level of selectivity and sensitivity by performing the following procedures:
Choosing the most compatible flow cell for your application Disassembling, cleaning, and installing the flow cell Changing the Deuterium lamp
Of course, you will also find the precision-engineered Agilent parts you need to get the job done quickly and keep your downtime to a minimum.
53
Detector Maintenance Tips

Symptom Lamp does not ignite Noise exceeds application limit Drift exceeds application limit Leaky flow cell (For G4212 only) Leaky flow cell (For all G1314/G1315/ G1365 detectors) Lower intensity (For G4212 only) Lower intensity (For all G1314/G1315/ G1365 detectors) What To Do Exchange the lamp Exchange the flow cell Exchange the lamp Exchange the flow cell Clean or exchange the flow cell Additional Information Perform a wavelength calibration test and an intensity test after the lamp replacement Perform a wavelength calibration test after the flow cell replacement Perform a wavelength calibration test and a pressure tightness test after the flow cell replacement Perform a wavelength calibration test after the flow cell replacement Perform a wavelength calibration test and a pressure tightness test after the flow cell replacement Perform a wavelength calibration test after the flow cell replacement Perform a wavelength calibration test and a pressure tightness test after the flow cell replacement
Exchange the flow cell Clean or exchange the flow cell
54
Tools required
Screwdriver (POZI 1 PT3)
Replacing the Deuterium Lamp

Preparations
1. Turn the lamp off. Note: Allow lamp to cool down. 2. Remove the front cover and locate the lamp area.
1. Unscrew the three screws of the lamp housing cover and remove the cover. 2. Locate the two screws that secure the lamp and unscrew. 3. Disconnect the lamp connector and remove the lamp. 4. Place the lamp on a clean surface. Note: Do not touch the glass bulb of the new lamp with fingers. Doing so may reduce light output.
Long Life Deuterium Lamp with RFID tag, 2140-0820
5. Insert the new lamp and reconnect the lamp connector. 6. Secure the lamp with the two screws. 7. Fit the lamp wires inside the lamp housing cover so that they are not scratched by the cover. 8. Replace the lamp housing cover and secure the three screws. Note: Perform a wavelength re-calibration after lamp warm up.
55
Tools required
Hexagonal wrench
Replacing the Max-Light Cartridge Cell in a 1290 Infinity LC

Preparations
1. Turn the pump off. Note: If the flow cell has not been used for some time, flush it with isopropanol. 2. Disconnect the inlet capillary to CELL IN (left) and the waste tube to CELL OUT (right).
1. Unlock the cell cartridge holder by pulling the lever to the front. The lever should be in the final down position. 2. Pull the cell cartridge holder completely out towards the front. 3. Remove the cell from the cartridge holder. 4. Replace the black hoods (A) to the cell interfaces (IN/OUT) and insert plugs (B) for safe storage. Note: The hoods and plugs should always be in place in order to protect the flow cell. For long-term storage, the flow cell should be flushed and filled with isopropanol to prevent the growth of algae. 5. Slide the cell cartridge holder completely into the module. 6. Lift the two levers into the upper final position to secure the cell. 7. Remove the plugs from the CELL IN and CELL OUT and set aside. 8. Connect the inlet capillary to CELL IN (left) and the waste tubing to CELL OUT (right).
56
Replacing the Flow Cell

Flow Cell Maintenance (Procedure A)
Standard flow cell, G1315-60012
1. Remove both capillaries from the flow cell. 2. Remove the flow cell from the holder. 3. Remove the cell screw with a 4 mm hex key. 4. Remove the gasket. 5. Use ethanol to clean the cell body. 6. Insert the new gasket. 7. Insert the new cell screw. 8. Carefully tighten the cell screw.
Flow Cell Maintenance (Procedure B)

1. Remove both capillaries from the flow cell. 2. Unscrew the cell screws and remove the flow cell. 3. Remove the screw with a 4 mm hex key. 4. Carefully remove the three washers and the gasket. 5. Carefully remove the cell window and the seal. 6. Use ethanol to clean the cell body. 7. Insert the new seal and the new cell window. 8. Insert the gasket and the three washers. 9. Insert the cell screw. 10. Carefully tighten the cell screw.
57
Installing the Detector Cell

Before you return the flow cell to the instrument module: 1. Connect the capillaries. 2. Switch on the pump and check the cell for leaks. Once you are convinced the cell is leak free: 3. Install the flow cell into the instrument. 4. Screw down the cell. We recommend the following tests after cell installation: Zero-order calibration Wavelength calibration Because the flow cell is part of the optical system and has now been moved since these tests were last performed, we recommend running them again before your next analysis.
58
Detector Lamps
Description Comments Part No. G1314-60101 G1314-60100
1100/1200 Series Variable Wavelength Detector (VWD)

Long life Deuterium lamp with RFID tag For G1314D/E Long life Deuterium lamp For G1314A/B/C/1120
1100/1200 Series Diode Array Detector (DAD)/Multiple Wavelength Detector (MWD)

Long life HiS Deuterium lamp (8-pin) with RFID tag Long life Deuterium lamp
Long Life Deuterium Lamp with RFID tag, 2140-0820
For G4212A
5190-0917 2140-0820 2140-0813 5181-1530 2140-0590 G1103-60001
Long life Deuterium lamp with RFID tag For G1315D/E and G1365D/E For G1315A/B/C and G1365A/B/C For G1315A/B/C and G1365A/B/C For G1315A/B/C and G1365A/B/C For G1315A/B/C/D/E and G1365A/B/C/D/E Long life Deuterium lamp Deuterium lamp Tungsten lamp
59
Flow Cell and Repair Kits for VWD

Description Standard flow cell, RFID Standard "D" type flow cell Semi-micro flow cell, RFID Semi-micro flow cell Micro flow cell, 3 mm, RFID Micro flow cell, 3 mm Micro flow cell, 5 mm High pressure flow cell, RFID High pressure flow cell
*See page 57 for repair procedures
Use With G1314DE G1314A/B/C G1314D/E G1314A/B/C G1314D/E G1314A/B/C G1314A/B/C G1314D/E G1314A/B/C
Comments 10 mm, 14 L, 40 bar 10 mm, 14 L, 40 bar 6 mm, 5 L, 40 bar 6 mm, 5 L, 40 bar 2 L, 120 bar 2 L, 120 bar 1 L, 40 bar 10 mm, 14 L, 400 bar 10 mm, 14 L, 400 bar
Part No. G1314-60086
Repair Kit Part No.
Repair Procedure* B A A
G1314-60186 G1314-65061 G1314-60183 G1315-68713 G1314-60083 G1314-60187 G1315-68713 G1314-60087 G1314-60081 G1314-60182 G1314-65054 G1314-60082
Capillaries for VWD Flow Cell

Use With Standard flow cell, RFID Standard "D" type flow cell Semi-micro flow cell, RFID Semi-micro flow cell Micro flow cell, 3 mm, RFID Micro flow cell, 3 mm Micro flow cell, 5 mm High pressure flow cell, RFID High pressure flow cell Inlet Capillary Inlet capillary, 600 mm, 0.17 mm ID Inlet capillary, 400 mm, 0.12 mm ID Inlet capillary, 310 mm, 0.12 mm ID Inlet capillary, 400 mm, 0.12 mm ID Inlet capillary, 380 mm, 0.17 mm ID Part No. 5062-8522 5021-1823 Outlet Capillary Waste capillary, PEEK, 0.25 mm ID Fingertight fitting Waste capillary, PEEK, 0.25 mm ID Fingertight fitting G1314-87301 Outlet capillary, 120 mm, 0.17mm ID 5021-1823 G1315-87311 Part No. 5062-8535 0100-1516 5062-8535 0100-1516 G1314-87302
60
Flow Cell and Repair Kits for DAD/MWD

Description Standard flow cell, RFID Standard flow cell Semi-micro flow cell, RFID Semi-micro flow cell Micro flow cell, RFID Micro high-pressure flow cell Preparative flow cell Max-light cartridge cell Max-light cartridge test cell
*See page 57 for repair procedures
Use With G1315C/D, G1365C/D G1315A/B, G1365A/B G1315C/D, G1365C/D G1315A/B, G1365A/B G1315C/D, G1365C/D G1315A/B, G1365A/B G1315A/B, G1365A/B G4212A Infinity DAD Must be used to perform detector build-IN tests
Comments 10 mm, 13 L, 120 bar 10 mm, 13 L, 120 bar 6 mm, 5 L, 120 bar 6 mm, 5 L, 120 bar 3 mm, 2 L, 120 bar 6 mm, 1.7 L, 400 bar 3 mm, 120 bar, stainless steel 10 mm, 1.0 L Must be used to perform detector build-IN tests
Part No. G1315-60012
Repair Kit Part No.
Repair Procedure* A A A A
G1315-60022 G1315-68712 G1315-60025 G1315-68713 G1315-60011 G1315-60024 G1315-68713 G1315-60015 N/A G1315-60016 G1315-68712 G4212-60008 N/A G4212-60011 N/A
Capillaries for DAD/MWD Flow Cell

Flow Cell Description Standard flow cell with RFID tag Standard flow cell Semi-micro flow cell with RFID tag Semi-micro flow cell Micro flow cell with RFID tag Micro high-pressure flow cell Part No. G1315-60022 G1315-60012 G1315-60025 G1315-60011 G1315-60024 Inlet Capillary Inlet capillary with heat exchanger, 0.17 mm ID, 590 mm long DAD heat exchanger capillary, 0.17 mm ID, 310 mm long Part No. Outlet Capillary Part No. G1315-87302 G1315-87321 Outlet capillary, 0.17 mm ID, 200 mm long
G1315-87319 Outlet capillary, 0.12 mm ID, 200 mm long Outlet capillary, 0.17 mm ID, 200 mm long Outlet capillary, 0.12 mm ID, 200 mm long Outlet capillary, 0.17 mm ID, 200 mm long G1315-87339 Outlet capillary, 0.12 mm ID, 200 mm long Outlet capillary, 0.17 mm ID, 200 mm long G1315-87325 Outlet capillary, 0.12 mm ID, 200 mm long
G1315-87306 G1315-87302 G1315-87306 G1315-87302 G1315-87306 G1315-87302 G1315-87306
DAD heat exchanger capillary, 0.12 mm ID, 310 mm long Inlet capillary with heat exchanger, 0.12 mm ID, 290 mm long
G1315-60015
61
80 nL and 500 nL Flow Cell Supplies

Description Fitting screw Double winged nuts and 1/32 in. ferrules 1/32 in. ferrule and SS lock ring, lite touch Union adjustment tool Universal ZDV union, stainless steel, no fittings Torque wrench adapter Open end wrench, 4 mm Unit 10/pk 10/pk 10/pk 2/pk 2/pk Part No. 5063-6593 5065-4422 5063-6592 5022-2146 5022-2184 G1315-45003 8710-1534
500 nL Flow Cell and Replacement Parts

Description 500 nL flow cell Comments Contains quartz flow cell with 10 mm path length and 500 nL volume and connecting capillaries, max 50 bar pressure Includes torque adapter, 2 cell seal assemblies, 5 litetouch front and back ferrules Part No. G1315-68724
Sealing kit
G1315-68715
Quartz cell body, 10 mm Cell seal assembly, 500 nL Fused silica/PEEK capillary, 100 m ID, 30 cm long Fused silica/PEEK capillary, 50 m ID, 40 cm long Fused silica/PEEK capillary, 100 m ID, 12 cm long Fused silica/PEEK capillary, 50 m ID, 12 cm long Inlet Inlet Outlet Outlet
G1315-80001 G1315-87101 G1315-87333 G1315-87323 G1315-87338 G1315-87328
500 nL flow cell and replacement parts
62
80 nL Flow Cell and Replacement Parts

Description 80 nL flow cell Comments Contains quartz flow cell with 6 mm path length and 80 nL volume and connecting capillaries, max 50 bar pressure Includes torque adapter, 2 cell seal assemblies, 5 litetouch front and back ferrules and 5 sleeves for 360 m OD capillaries Part No. G1315-68716
Sealing kit for 80 nL flow cell
G1315-68725
Quartz cell body, 80 nL, 6 mm path length Fused silica/PEEK capillary, 50 m ID, 40 cm long Fused silica/PEEK capillary, 50 m ID, 12 cm long Fused silica/PEEK capillary, 25 m ID, 20 cm long Fused silica/PEEK capillary, 25 m ID, 60 cm long Inlet Outlet Inlet Outlet
G1315-80002 G1315-87323 G1315-87328 G1315-87313 G1315-87318
Preparative Flow Cells and Replacement Parts

Description Prep flow cell, quartz, 0.3 mm, 20 bar Prep flow cell, quartz, 0.06 mm, 20 bar PTFE tubing, 0.8 mm ID, 2 m PTFE tubing, 0.5 mm ID, 0.8 m Cell housing 1/16 in. finger-tight PEEK fittings, beige Quartz body, 0.3 mm Quartz body, 0.06 mm Prep flow cell stainless steel, 3 mm, 120 bar Stainless steel connecting capillary, 0.5 mm, 250 mm Part No. G1315-60017 G1315-60018 G1315-67301 G1315-67302 G1315-27705 0100-1516 G1315-80004 G1315-80003 G1315-60016 G1315-87305
63
Detector Maintenance Kits

Description G1314A VW detector accessory kit Comments Part No.
1100/1200 Series Variable Wavelength Detector (VWD)

Includes 1/4 in. waste tubing, 2 hex keys, G1314-68705 2 wrenches, outlet tubing, 1/16 in. PEEK male fitting Includes 2 windows, 2 gaskets #1, 2 gaskets #2 Includes 2 windows, 4 gaskets: 2 standard #1, 1 semi-micro #1, 1 semi-micro #2 Includes 2 windows, 2 gaskets #1, 2 gaskets #2 Includes window screw kit, 4 mm hexagonal wrench and seal kits Includes 2 windows, 2 Kapton gaskets and 2 PEEK rings G1314-65061 G1314-65056
Standard "D" type flow cell kit Semi-micro flow cell kit
Micro flow cell kit Cell repair kit, semi-micro High-pressure flow cell kit
G1314-65052 G1315-68713 G1314-65054
1100/1200 Series Diode Array Detector (DAD)/ Multiple Wavelength Detector (MWD)
Cell repair kit Cell repair kit, semi-micro Sealing kit for 500 nL flow cell Includes window screw kit, 4 mm hexagonal wrench and seal kit Includes window screw kit, 4 mm hexagonal wrench and seal kits Includes torque adapter, 2 cell seal assemblies, 5 litetouch front and back ferrules Includes torque adapter, 2 cell seal assemblies, 5 litetouch front and back ferrules and 5 sleeves for 360 m OD capillaries G1315-68712 G1315-68713 G1315-68715
Sealing kit for 80 nL flow cell
G1315-68725
64
G4218A 1200 Series Evaporating Light Scattering Detector Supplies

Description Standard flow nebulizer Semi-micro flow nebulizer Large flow nebulizer Micro flow nebulizer RRLC nebulizer Nebulization chamber, glass Black plastic nut, 13 mm diameter, glassware Black plastic nut, 22 mm diameter, glassware Black exhaust tube, 2.5 m Bulkhead Cartridge, 0.01 m for gas regulator Pneumatic tube with stainless steel fitting Drain tube with stainless steel fitting Gas regulator with 0.01 m filter and manometer Seal kit for nebulization chamber Caffeine standard, 250 g/mL Part No. G4218-20000 G4218-20001 G4218-20002 G4218-20003 G4218-20004 G4218-40000 G4218-40010 G4218-40011 G4218-40110 G4218-40130 G4218-40150 G4218-40220 G4218-40100 G4218-60100 G4218-68010 G4218-85000
65
G1362A 1100/1200 Series Refractive Index Detector (RID) Supplies

Description Tubing kit Includes 300 mm recycle valve to recycle port, 200 mm recycle valve to waste port, 120 mm purge valve to recycle valve, 270 mm purge valve to sample cell, 170 mm purge valve to reference cell Interface tubing kit Includes 1/8 in. ferrule, 1/3 in. nut, PTFE tubing Interface capillary, 400 mm, 0.17 mm ID Restriction capillary, 0.17 mm ID Part No. G1362-68709
G1362-68706 G1362-87300 G1362-87301
66
G1321A 1100/1200 Series Fluorescence Detector (FLD) Supplies

Description Xenon flash detector lamp
Stainless steel front ferrules, 5180-4108
Part No. 2140-0600 G1321-60005 G1321-60007
Flow cell Cuvette kit, 8 L, 20 bar Includes tubing, SS fitting, front and back ferrule, PEEK fitting, syringe needle and syringe Cut-off filter kit: 389, 408, 450, 500, 550 nm 380, 399, 418, 470, 520 nm 280, 295, 305, 335, 345 nm Corrugated tubing, polypropylene, 6.5 mm ID, 5 m PTFE tubing, FEP, 0.7 mm ID, 5 m 1/16 in. finger-tight PEEK fittings, beige, 2/pk Column connecting capillary with fittings, 380 x 0.17 mm 1/16 in. front ferrule, stainless steel, 10/pk 1/16 in. back ferrule, stainless steel, 10/pk 1/16 in. male fittings, stainless steel, 10/pk Hex key, 4.0 mm, 10 cm long straight handle Hex key, 2.5 mm, 10 cm long straight handle Fluorescence detector calibration sample, 1 g glycogen Hex key set, 1-5 mm Open end wrench, 1/4 and 5/16 in. Glass syringe Syringe needle
5061-3327 5061-3328 5061-3329 5062-2463 5062-2462 0100-1516 G1315-87311 5180-4108 5180-4114 5061-3303 5965-0027 5965-0028 5063-6597 8710-0641 8710-0510 9301-1446 9301-0407
Flow cell for G1321A fluorescence detector, G1321-60005
Back ferrule 1/16 in., 5180-4114
67
1100/1200 Series Chip LC Supplies

Description Rotor, inner valve, 3 grooves, chip LC Rotor, outer valve, 5 grooves, chip LC PEEK fitting, special for chip LC Fused silica/PEEK capillary, 15 m, 90 cm Nano pump to chip cube Fused silica/PEEK capillary, 25 m, 105 cm Micro well plate sampler to chip cube Fused silica/PEEK capillary, 100 m, 100 cm Chip cube to waste Fused silica/PEEK capillary, 75 m, 100 cm Syringe pump to chip cube Fused Silica/PEEK capillary, 50 m, 50 cm Inline micro filter kit, 0.5 m, PEEK Use with chip cube LC system Fitting with 0.5 m PEEK frit, 10/pk PEEK fitting for use with 1/32 in. OD, 10/pk Part No. G4240-23705 G4240-25206 G4240-43200 G4240-87300 G4240-87301 G4240-87302 G4240-87303 G4240-87304 5067-1582 5067-1584 5067-1585
68
LC/MS System Maintenance

A higher level of purity for results that stand up to scrutiny.
Combined with Agilent's industry-leading LC systems, our single quadrupole, ion trap, triple quadrupole, TOF and Q-TOF LC/MS solutions combine world-class performance with legendary reliability and ease-of-use. This section contains essential information to keep your LC mass spectrometer running at peak performance, including:
LC/MS maintenance, including routine procedures Supplies, chemicals, calibrants, and tools for maintaining your system Quiet Cover II for easy movement and maintenance of your rough pumps
69
LC/MS Maintenance Schedule

Procedure Flush the nebulizer Clean the electrospray spray chamber Replace the electrospray nebulizer needle Clean the APCI spray chamber Replace the APCI nebulizer needle Clean the multimode source When to Perform Daily or at the end of each shift to flush traces of samples and buffers out of the tubing, valves, and nebulizer. Daily or anytime you suspect carryover contamination from one sample or analysis to another. When the needle is plugged. Common symptoms of a plugged needle are increased LC back pressure, off-axis spraying, or dripping from the nebulizer. Daily or anytime you suspect carryover contamination from one sample or analysis to another. When the needle is plugged. Common symptoms of a plugged needle are increased LC back pressure or off-axis spray from the nebulizer. Daily or anytime you suspect carryover contamination from one sample or analysis to another, or when you must access the end cap and capillary cap for cleaning and inspection. Monthly or weekly if you tune the LC/MS frequently.
Check calibrant levels
70
LC/MS Supplies
Description ES nebulizer assembly Used for analytical sprayer API-ES nebulizer needle replacement kit APCI nebulizer assembly APCI nebulizer needle replacement kit Needle assembly Corona needle APCI Capillary cap (G1946A/B/C) Capillary cap (G1946D and G1956A/B) Capillary (G1946A/B/C and G1956A) Capillary (G1946D, G1956B) Capillary (G6140A, G6460A, G6530A) Spring canted coil, 0.25 in. ID, 0.53 mm 1/6 in. tee, low dead volume, stainless steel ] ] ] ] 6100 Series Single Quadropole LC/MS ] ] ] ] ] ] ] ] ] ] ] ] ] ] ] ] ] ] ] ] ] ] ] 6200 Series TOF LC/MS ] ] ] ] ] ] 6300 Series Ion Trap LC/MS ] ] ] ] ] ] ] 6400 Series Triple Quadropole LC/MS ] ] ] ] 6500 Series Accurate-Mass Q-TOF LC/MS Part No. ] G1946-60098 ] ] ] ] ] G2427A G1946-60037 G2428A G1947-60103 G1947-20029 G1946-20056 G1946-20301 G1946-80009 59987-20040 G1960-80060 1460-2571 0100-0969
71
LC/MS Supplies
Description Syringe adapter Syringe pump
(Continued)
6100 Series Single Quadropole LC/MS 6200 Series TOF LC/MS 6300 Series Ion Trap LC/MS ] ] ] ] ] ] ] ] ] ] ] ] ] ] ] ] ] ] ] ] ] ] ] ] ] ] ] ] 6400 Series Triple Quadropole LC/MS 6500 Series Accurate-Mass Q-TOF LC/MS Part No. 9301-1291 3162-0178 0100-1516 0100-2304 0890-1915 5182-9710 G1969-20302 G1946-20089 G1946-20302 G1956-20302 G1946-20087 G1946-80019 G2571-80103 G1956-80000 05971-80103 G2441-80010
1/16 in. finger-tight PEEK fittings, beige Female luer to female 10/32 adapter PEEK tubing Gas-tight syringe, PTFE luer lock High-throughput skimmer, 2 mm Skimmer 1 (G1946A/B/C) Skimmer 1 (G1946D) Skimmer 1 (G1956A/B) Skimmer 2 (G1946A/B/C and G1956A only) HED electron multiplier (G1946A/B/C/D) HED electron multiplier (G6140A, G6460A, G6530A) Detector assembly (G1956A/B) Electron multiplier replacement horn Replacement horn and dynode
72
LC/MS Chemicals
Description ES/APCI positive ion performance standard, 5 x 1 mL ampoules Electrospray LC demo sample (Sulfamix) ESI+APCI LC demo sample ES Negative Ion Performance Standard, 5 x 1 mL ampoules APCI Negative Ion Performance Standard, 5 x 1 mL ampoules ES/APCI Positive Ion Performance Standard Multiple-charge compound performance evaluation sample (horse heart myoglobin) Caffeine Standards Kit for LC/MS OQ/PV ES/APCI positive ion performance standard, 5 x 1 mL ampoules ES-TOF reference mix, 6 x 2 mL ampoules ES-TOF Biopolymer reference standard kit Flushing solvent High purity water, 4 L Methyl alcohol, 1 L Ammonium formate Formic acid, 5 mL Acetonitrile, 1 L
*Recommended item for familiarization
6100 Series Single Quadropole LC/MS ] ] ] ] ] ] ] ] ]
6200 Series TOF LC/MS ]
6300 Series Ion Trap LC/MS
6400 Series Triple Quadropole LC/MS ] ]*
6500 Series Accurate-Mass Q-TOF LC/MS Part No. ] G2423A 59987-20033 G1978-85000
G2424A G2425A G1946-85004 G2426A
8500-6917 G2423A ] ] ] G1969-85001 G1969-85003 ] ] ] ] ] ] ] ] ] ] ] ] ] ] ] ] ] ] G1969-85026 8500-2236 8500-1867 G1946-85021 G2453-85060 G2453-85050
] ] ] ] ] ]
] ] ] ] ] ]
73
LC/MS Calibrant Mixes

Description ESI tuning mix, 100 mL APCI/APPI calibrant solution, 100 mL ESI tuning mix for ion trap, 100 mL ES-TOF tuning mix, 100 mL APCI-L low concentration tuning mix, 100 mL MMI-L low concentration tuning mix, 100 mL Part No. G2421A G2432A G2431A G1969-85000 G1969-85010 G1969-85020
LC/MS Calibrant Mix and Source Compatibility Matrix

6100 Series Single Quadropole LC/MS G2421A G2432A G2432A G2432A 6140 Series Single Quadropole LC/MS G1969-85000 G1969-85010 G2432A G1969-85000 G1969-85000** 6200 Series TOF LC/MS G1969-85000 G1969-85010 G1969-85010 G1969-85020 G1969-85000**** G1969-85000**** 6300 Series Ion Trap LC/MS G2431A G2432A G2432A G2432A G2431A G2431A* G1969-85000*** 6400 Series Triple Quadropole LC/MS G1969-85000 G1969-85010** G2432A** G1969-85020 6500 Series Accurate-Mass Q-TOF LC/MS G1969-85000 G1969-85010** G1969-85010** G1969-85020 G1969-85000**** G1969-85000****
Source ESI APCI APPI MMI NanoESI HPLC chip cube

*5X dilution suggested **No autotune ***ESI positive tune only ****Calibration only
74
LC/MS Pump Supplies

Description Oil mist filter kit for E2M18 Oil return kit Foreline pump oil, Inland 45, 1 L Rotary pump oil, 4 L Foreline exhaust adapter Hose clamp Oil mist filter element for E2M18 KF25 clamp, stainless steel KF25 coseal (inside clamp) Exhaust tubing Pump oil drip pan Part No. 3162-1056 3162-1057 6040-0834 6040-0798 59980-20134 1400-0563 1535-4970 0100-0549 0100-1597 0890-1727 G1946-00034
75
LC/MS Common Supplies*

Description
Common Parts
Part No. 0100-2051 0100-1854 0100-1855 G1946-60180 G1969-60086 1460-2571 8660-0827 G1946-80054 5190-1401 05980-60051 5080-5400 BHT-4 RMSN-2 5183-2003 5183-2004 5183-2014
Filter element, 5 m, 5/pk Rotor seal, Tefzel, pH 0 to 14 Rotor seal, Vespel, pH 0 to 10 Inlet filter assembly SSV long drain tubing assembly Spring canted coil
Cleaning Supplies
Abrasive mesh, 4000 grit Capillary cleaning wire for dip tube Cleaning powder, dielectric capillary, Alconox Cloths, lint free Cotton swabs, 100/pk
Gas Purifiers and Generators
Nitrogen gas generator, 5183-2003
Big hydrocarbon trap, 1/4 in. fittings Big universal trap, 1/8 in. fittings, nitrogen Nitrogen gas generator Nitrogen gas generator with oxygen monitor, 120/220V Maintenance kit for LC MSD nitrogen generator
*These parts are common to all LC MSD systems
76
LC/MS Common Supplies*

Description
Tools
(Continued)
Part No. G1946-60157 G1946-20215 G1946-80049 8710-1930 8710-1622 8710-1615 8710-0510 8710-0806 8710-0004 8710-2699 5190-1443
G1946A tool kit Nebulizer adjustment fixture

Nebulizer 25X magnifier Plastic tubing cutter Screwdriver, Torx T15 Screwdriver, Torx T20 Open end wrench, 1/4 and 5/16 in. Wrench, 1/2 and 7/16 in. Needle nose pliers, pointed serrated jaws 3 mm wrench for nebulizer needle adjustment
Maintenance Kit
LC/MS Preventive Maintenance kit Includes vacuum pump oil, oil element, big hydrocarbon trap, 5 m filter, rotor seal and canted coil spring
*These parts are common to all LC MSD systems
77
Quiet Cover
Agilent has a solution to the frequent maintenance and inherent noise of LC/MS rough pumps. The Quiet Cover II was designed for easy movement, maintenance, and better living with rough pumps used with Agilent and other LC/MS systems.
Locking castors to move heavy pump for maintenance No tools necessary to remove sectioned cover for easy access to pump Built in Lift and Tilt lever raises end of pump to drain oil Removable drip pan with well and hand holds to collect and transport oil Sound absorbing cabinet with resistant foam insulation to reduce pump noise Pump mounted on cushioned grommets to minimize vibration 2 Integrated fans maintain temperature inside cover LEDs and audible alarm if temperature exceeds 35 C limit Maximum ambient temperature of 35 C when airflow is neither restricted nor recycled Standard one-year warranty. Installation and familiarization included with new LC/MS orders
The Quiet Cover II is compatible with these Agilent LC/MS Systems:
6100 Single Quads: G6110AA, G6120AA, G6130AA, G6140AA 6300 Traps: G2440DA, G2451AA, G4533AA, G2474SS 6410 QQQ: G6410AA 6210 TOF: G3250AA, G3252A 6510AA Q-TOF: G6510AA
Or any analytical system using BOC Edwards pumps (lbs/kg): E2M28, E2M18, E1M18.
Quiet Cover
Quiet Cover II for Agilent LC/MS Systems

Description Quiet Cover II for Agilent LC/MS Systems 12.5 W x 17.3 H x 33.5 L Part No. G3199B
78
General Supplies
They may be small, but they have a BIG effect on your productivity and results.
While supplies and accessories are the least expensive components of your LC system, they can contribute to problems such as injector damage, ghost peaks, and analyte degradation. That is why Agilent supplies are engineered with the same reliability and reproducibility you expect from Agilent instruments. And they are precisely designed to work with your 1100, 1120, 1200 or 1290 Series HPLC System. The following pages feature a wide selection of Agilent-engineered supplies, including:
Connection capillaries Fittings and unions LC tubing In-line and solvent filters Frits and adapters Sample filtration supplies
These supplies are also designed for fast, easy replacement, so you can get your system running again quickly.
79
General Supplies
Made of flexible stainless steel (0.6 mm OD) with 1/16 in. OD tubing at both ends to accept standard fittings Pre-swaged fittings are assembled according to Swagelok specifications
Capillaries for 1100 and 1200 LC Systems

From Pump To Autosampler ID (mm) 0.17 0.25 0.25 0.17 Manual valve Universal 1 multi assembly 1 multi assembly Restriction capillary SSV/MCGV OBV Mixing capillary Damper Damper Filter Needle seat EMPV EMPV Union Union EMPV1 AIV Piston 2 Damper Mixer Purge valve EMPV Injection valve Next module Multi assembly EMPV2 Mixer Union 0.6 0.5 0.6 0.6 367 400 160 111 40 0.6 0.6 0.17 0.25 Pre-swaged 230 495 130 0.17 0.25 0.6 0.6 Length (mm) Material* 600 130 320 500 900 700 173 175 SS SS SS SS SS SS SS SS SS PTFE SS SS SS SS SS SS SS SS SS SS SS Green Blue Color Code Fittings/Connection Green Blue Blue Green Green Blue Pre-swaged Pre-swaged Pre-swaged Non-swaged 1 end pre-swaged 1 end pre-swaged Male to male Male to male A/A Pre-swaged Pre-swaged Pre-swaged Pre-swaged A/A A/A -C Male to male Male to male Male to female Male to female Male to female Part No. G1312-67305 01090-87308 79835-87638 G1328-87600 G1329-87300 01018-67305 G1361-67300 G1361-67301 G1312-67302 G1311-67304 G1312-67300 G1312-67304 01090-87308 G1312-67301 G1375-87400 G1329-87101 G1361-67302 G1361-67303 G1361-67304 G1361-67305 G1361-67306
80
General Supplies
Capillaries for 1100 and 1200 LC Systems

From Autosampler To Column ID (mm) 0.12 0.12 0.12 0.17 0.17 0.17 0.17 0.5 Manual valve Column compartment Detector Column Column Column Column Waste DAD VWD 0.17 0.12 0.17 0.12 0.17 0.18 180 280 105 180 280 800 130 600 500 70 90 150 380 40
(Continued)
Color Code Fittings/Connection Red Red Red Green Green Green Green Green Red Green Red Green 1 end pre-swaged 1 end pre-swaged 1 end pre-swaged 1 end pre-swaged 1 end pre-swaged 1 end pre-swaged 1 end pre-swaged Non-swaged 1 end pre-swaged Non-swaged Pre-swaged Pre-swaged Part No. G1313-87304 01090-87610 01090-87611 G1313-87305 01090-87304 01048-87302 01090-87305 G2260-87300 G1328-87600 G1316-87303 G1316-87300 G1315-87312 G1315-87311 5062-8522 SS SS SS SS SS SS SS SS SS SS SS SS SS PEEK
Length (mm) Material*
Inlet capillary, With fittings 600 mm, 0.17 mm ID Red Red Red Red Red Green Green Green Green Green Without fittings Without fittings Without fittings Without fittings 1 end pre-swaged Without fittings Without fittings Without fittings Without fittings With fittings
0.12 0.12 0.12 0.12 0.12 0.17 0.17 0.17 0.17 0.17 VWD Waste 0.25
105 150 280 400 70 105 150 280 400 90 48
SS SS SS SS SS SS SS SS SS SS PEEK
5021-1820 5021-1821 5021-1822 5021-1823 G1316-87303 5021-1816 5021-1817 5021-1818 5021-1819 G1316-87300 5062-8535
Waste Without fittings capillary, PEEK, 0.25 mm ID Blue Color Code Fittings/Connection Red Green Red Pre-swaged Pre-swaged 1 end pre-swaged
Injector valve Injector valve Description Stainless steel capillary Stainless steel capillary Stainless steel capillary
*SS = stainless steel
Waste Prep head
0.25 0.5 ID (mm) 0.12 0.17 0.12
120 160 210 250 250
SS SS SS SS SS
G1377-87301 G2258-87301 Part No. G1316-87328 G1367-87304 G1373-87300
Length (mm) Material*
81
General Supplies
1200 Rapid Resolution Stainless Steel Connection Capillaries

From Pump Damper Damper Mixer Column Cell out DAD heat exchanger capillary DAD heat exchanger capillary Valve Micro valve WPS Micro valve TCC VWD Column Valve Regeneration pump Micro valve Heat exchanger MS DAD cell To Autosampler Cooled autosampler Pressure sensor Mixer Outlet valve Flow cell MS ID (mm) 0.17 0.17 0.17 0.17 0.17 0.12 0.17 0.12 0.12 0.12 0.12 0.12 0.12 0.12 0.12 0.12 0.12 0.17 WPS TCC 0.12 0.12
*0.8 mm OD stainless steel capillaries use 0.8 mm ID fittings
Length (mm) Connection 400 700 150 105 150 500 310 310 100 130 340 75 500 50 70 90 130 150 170 210 Male to male Male to male Male to male Male to male Male to female Male to male Male to male Male to male Male to male Male to female Male to male Male to male Male to male Male to female Male to female Male to female Male to female Male to female Male to male Male to male
Fittings Pre-swaged Pre-swaged Pre-swaged Pre-swaged Non-swaged 1 end pre-swaged 1 end pre-swaged 1 end pre-swaged Non-swaged Non-swaged 1 end pre-swaged Non-swaged 1 end pre-swaged Non-swaged Non-swaged Non-swaged Non-swaged Non-swaged Non-swaged Non-swaged
Part No. G1312-87303 G1312-87304 G1312-87305 G1312-87306 G1315-87303 G1315-87307 G1315-87319 G1315-87339 G1316-27301* G1316-87304* G1316-87305* G1316-87306* G1316-87309 G1316-87312 G1316-87313 G1316-87314 G1316-87315 G1315-87303 G1316-87316 G1316-87317
82
General Supplies
1200 Rapid Resolution Stainless Steel Connection Capillaries

From Cooled WPS Column Micro valve Column WPS 10 port valve WPS Description DAD heat exchanger capillary DAD heat exchanger capillary Absorber capillary, 500 L Calibration capillary assembly Stainless steel restriction capillary, T-piece to pressure sensor Column connecting capillary with fittings Flexible capillary Stainless steel capillary Stainless steel capillary Stainless steel capillary
*0.8 mm OD stainless steel capillaries use 0.8 mm ID fittings
(Continued)
Part No. G1316-87318 G1316-87319 G1316-87321 G1316-87323 G1316-87326* G1316-87327 G1316-87328* G1367-87303* G1367-87304 Part No. G1315-87319 G1315-87339 G1312-87300 G1312-67500 G1312-87301
To TCC Cooler (50-150 mm column) Cooler (20-30 mm column) Detector DAD cell 10 port valve Column TCC
ID (mm) 0.12 0.12 0.17 0.17 0.12 0.12 0.12 0.12 0.17 ID (mm) 0.17 0.12 0.17 0.17 0.17 0.17 0.17 0.17 0.17
Length (mm) Connection 300 340 105 170 75 170 210 100 250 310 310 1770 Male to male Male to male Male to male Male to male Male to female Male to female Male to male Male to male Male to male Male to male Male to male Male to male
Fittings Non-swaged Non-swaged Non-swaged Non-swaged Non-swaged Non-swaged Pre-swaged Non-swaged Pre-swaged Fittings 1 end pre-swaged 1 end pre-swaged Pre-swaged
Length (mm) Connection
90 280 230 320 150
Male to male Male to male Male to male Male to female
Non-swaged Without fittings Pre-swaged Pre-swaged Pre-swaged
G1316-87300 5021-1818 5067-1570 5067-1571 5067-1572
83
General Supplies
1290 Infinity LC Stainless Steel Connection Capillaries

From Pump Pump Autosampler TCC 1290 LC System To Autosampler Thermostatted Autosampler TCC DAD CTC Autosampler Valve Valve HX1-HX2 Valve (short) Valve (long) Valve Detector Purge valve Purge valve Purge valve Metering device ID (mm) Length (mm) 0.17 0.17 0.12 0.12 0.17 0.12 0.12 0.17 0.12 0.12 0.12 0.12 0.12 0.25 0.25 0.17 0.17 300 450 340 220 600 600 340 700 90 150 280 120 200 250 80 140 160 Fittings Pre-swaged Pre-swaged Non-swaged Non-swaged Pre-swaged Non-swaged Pre-swaged Pre-swaged Pre-swaged Non-swaged Non-swaged Pre-swaged Pre-swaged Pre-swaged Pre-swaged Pre-swaged Pre-swaged Part No. 5067-4657 5067-4658 5067-4659 5067-4660 5067-4670 5067-4669 5067-4647 5067-4648 5067-4649 5067-4650 5067-4651 5067-4652 5067-4653 5067-4655 5067-4656 G4220-87000 G4226-60301
CTC Autosampler Column Autosampler Second pump Valve Column Column Valve Valve Filter Pressure sensor Mixer Valve
84
General Supplies
PEEK Coated Fused Silica Capillaries for use with 20 L/min Flow Range
From EMPV Flow sensor Injection valve Metering device Injection valve Column Detector -switching valve To Flow sensor Injection valve Metering device Needle Column Detector Waste Column ID (m) 50 50 50 100 50 50 75 50 Length (mm) 220 550 200 110 500 400 700 280 Fitting Part No. B/B B/C B/C B/B C/D D/E E/C/D G1375-87301 G1375-87310 G1375-87302 G1375-87303 G1375-87304 G1315-68703 G1315-68708 G1375-87309
PEEK Coated Fused Silica Capillaries for use with 100 L/min Flow Range
From EMPV Flow sensor Injection valve Metering device Injection valve Column Detector -switching valve To Flow sensor Injection valve Metering device Needle Column Detector Waste Column ID (m) 100 100 100 100 75 75 75 50 Length (mm) 220 550 200 110 500 400 700 280 Fitting Part No. B/B B/C B/C B/B C/D D/E E/C/D G1375-87305 G1375-87306 G1375-87312 G1375-87303 G1375-87311 G1375-87308 G1315-68708 G1375-87309
85
General Supplies
Replacement Fittings and Ferrules for Capillary and Nano Flow System
Graphic Description 1/16 in. stainless steel fittings, front and back ferrules, 10/pk 1/16 in. stainless steel fittings, male, 4 mm, 10/pk Type A Part No. 5062-2418
5063-6593
1/32 in. ferrule and stainless steel lock ring, 10/pk 6 fittings, 2 plugs, PEEK for -valves
5065-4423
5065-4410
Double winged PEEK nuts and 1/32 in. ferrules, 10/pk
5065-4422
PEEK fitting, long for 1/32 in. OD capillary
5022-6536
PEEK Coated Fused Silica Capillaries for Nano LC

From Switching valve EMPV Flow sensor Injection valve Switching valve Switching valve Switching valve Injection valve To Column Flow sensor Injection valve Flow sensor Column Column Column Injector seat or 2nd pump 25 50 75 700 100 650 C/D C/D C/D G1375-87324 G1375-87325 G1375-87327 ID (m) 25 25 25 25 Length (mm) 100 220 350 550 Fitting Part No. C/D B/B B/C C/D G1375-87320 G1375-87321 G1375-87322 G1375-87323
86
General Supplies
Fittings for 1/16 in. OD Capillaries

Description
Stainless steel fittings, 5062-2418
Part No. 5062-2418 5065-4454 5065-9967 5061-3303 5180-4108 5180-4114 5063-6591 0100-1516 5062-8541 5065-4426 5042-6500 0100-1631 0100-2175 0350-1402 01080-83202 5067-1540 5067-1547 0100-1259 5042-8957 5067-1557 5067-1558
1/16 in. stainless steel fittings, front and back ferrules, 10/pk 1/16 in. stainless steel long fittings, front and back ferrules, 10/pk 1/16 in. extra long stainless steel fittings and ferrules, 10/pk 1/16 in. male fittings, stainless steel, 10/pk 1/16 in. front ferrule, stainless steel, 10/pk 1/16 in. back ferrule, stainless steel, 10/pk 1/16 in. finger-tight PEEK fittings, beige, 10/pk 1/16 in. finger-tight PEEK fittings, beige, 2/pk 1/16 in. finger-tight PEEK long fittings, beige, 10/pk 1/16 in. finger-tight PEEK fittings, 10/pk 2 each: yellow, blue, black, green, red 1/16 in. double winged 2 piece fitting, 10/pk PEEK RheFlex 2-piece fittings, 5/pk PEEK RheFlex 2-piece fittings, colored, 10/pk ChromTrac identifiers, 20/pk 2 each: black, green, white; 4 each: yellow, blue, red 1/16 in. blanking nut, stainless steel Stainless steel hex nut fitting, PEEK ferrule, max 600 bar, 6/pk 1/16 in. PEEK ferrule, max 600 bar, 6/pk, for use with 5067-1540 fitting 1/16 in. plastic fitting (plug)
Stainless steel long fittings, 5065-4454
PEEK fittings, 5063-6591
PEEK RheFlex fittings, 0100-1631
PEEK long fittings, 5062-8541
1/16 in. finger-tight polyketone fittings, max pressure 600 bar, 10/pk 0.8 mm ID stainless steel ferrules, 6/pk 0.8 mm ID stainless steel fittings, M4/4 mm, 6/pk
Finger-tight PEEK fittings, 5065-4426
ChromTrac identifiers, 0350-1402
87
General Supplies
Unions
Description True ZDV union, no fittings
Micro T-connector, PEEK, 5042-8519
Use With Nano LC Capillary/Nano/Standard LC Standard LC Bio applications Prep LC
Part No. 5022-2145 5022-2184 0100-0900 0100-2441 5022-2133 0100-1847 0100-2298 5065-9971 5042-8517 5042-8518
Universal ZDV union, stainless steel, no fittings ZDV union, with fittings ZDV union, PEEK with fittings High flow union, no fittings PEEK adapter 1/4-28 to 10-32
ZDV Union with Fittings, 0100-0900
Adapter, PEEK int. 1/4-28 to ext. 10-32 Barbed Y-Connector PP for 3/16 in. ID tube, 10/pk Adapter, female to female 1/4-28 Adapter, male luer to female 1/4-28 T-connector, PEEK, swept volume 0.57 L For 1/16 in. OD tubing
5022-2144 5042-8519
High Flow Union, 5022-2133
Micro T-connector, PEEK, swept vol 29 nL, with 1/32 in. ID fittings
88
General Supplies
Flexible Stainless Steel Capillaries Without Fittings

ID (mm) 0.12 Length (mm) 105 150 200 280 400 500 0.17 105 150 200 280 400 600 700 900 0.25 250 280 320 800 0.50 105 150 280 800 Color Code Red Red Red Red Red Red Green Green Green Green Green Green Green Green Blue Blue Blue Blue None None None None Volume (L) 1.2 1.7 2.3 3.2 4.5 5.6 2.4 3.4 4.6 6.4 9.1 13.6 15.9 20.5 12.3 13.8 15.8 39.3 20.6 29.5 55 157 Part No. 5021-1820 5021-1821 5065-9935 5021-1822 5021-1823 5065-9964 5021-1816 5021-1817 5065-9931 5021-1818 5021-1819 5065-9933 5065-9932 5065-9963 5065-9979 5022-6508 5065-9980 5065-9930 5065-9927 5022-6509 5022-6510 5065-9926
PEEK Tubing
ID (mm) 0.50 0.25 0.25 0.18 0.18 0.13 0.13 Length (m) 1.5 1.5 5 1.5 5 1.5 5 Color Code Orange Blue Blue Yellow Yellow Red Red Part No. 0890-1761 0890-1762 5042-6463 0890-1763 5042-6462 0890-1915 5042-6461
89
General Supplies
Other Tubing
Description PTFE tubing, FEP, primary use for valve solutions PTFE solvent tubing, primary use for flow path from solvent bottle to degasser, to pump Corrugated tubing, polypropylene Silicone tubing Clamps and micro clamps, 10/pk Barbed Y-Connector PP for 3/16 in. ID tube, 10/pk Front seat tube, SS Back seat tube, SS Front seat tube, PTFE Back seat tube, PTFE Waste tube Waste tube Drawing tube assembly for flush solvent with filter and bottle cap Tubing assembly, solvent flush Waste tube Waste tubing, 5 m, 6.5 mm ID, corrugated polypropylene Waste tube, FEP For G1313/27/29A 1100/1200 Series Autosampler For G1387A 1100/1200 Series Micro Autosampler 5 6.5 0.8 1.6 For G2258A 1100/1200 Series Dual Loop Autosampler 0.1 0.12 0.1 0.12 0.15 0.1 0.5 0.5 0.2 0.25 0.8 0.8 Comments Length (m) 5 5 5 5 ID (mm) 0.7 1.5 6.5 1 3 OD (mm) 1.6 3.1 Part No. 5062-2462 5062-2483 5062-2463 5065-9978 5065-9976 5065-9971 G2258-87316 G2258-87315 G2258-87312 G2258-87313 G2258-87310 G2258-87311 G2258-87307 G2258-87314 G1313-87300 5062-2463 G1375-87326
90
General Supplies
Accessories
Description Plastic tubing cutter Blades for plastic tubing cutter, 5/pk
Part No. 8710-1930 8710-1931 5065-9948 5065-9950 0100-2410 G1313-43216
Fitting screws, SS, 10-32, 4 mm, 5/pk PEEK ferrule and stainless steel ring for 2 mm tube, 5/pk Union, PEEK for 1/8 in. OD tubing Waste adapter, 1200 Series autosamplers, gray
Capillary and Fittings Kits

Description SS flexible capillary tubing kit SS flexible capillary tubing kit Capillary/fitting starter kit, 0.17 mm ID Contents Includes 0.12 mm ID capillaries: 3 each 105 mm long, 1 each 150 mm long, 1 each 280 mm long, plus fittings Includes pre-swaged 0.12 mm ID capillaries: 3 each 105 mm long, 1 each 280 mm long, 1 each 35 mm long Kit includes: Qty 1 PEEK capillary 0.18 mm ID, 1.5 m Qty 4 Stainless steel capillary, 105 x 0.17 mm Qty 4 Stainless steel capillary, 150 x 0.17 mm Qty 2 Stainless steel capillary, 200 x 0.17 mm Qty 2 Stainless steel capillary, 280 x 0.17 mm Qty 1 Stainless steel capillary, 400 x 0.17 mm Tubing cutter for PEEK capillaries 1/16 in. stainless steel fittings, 10/pk 1/16 in. PEEK fittings, color, 10/pk 1/16 in. PEEK fittings, 10/pk Qty 3 ZDV union, stainless steel Rheotool Cybertool Part No. 5061-3304
5061-3315
5065-9939
91
General Supplies
Capillary and Fittings Kits

Description Capillary/fitting starter kit, 0.12 mm ID Contents
(Continued)
Part No. 5065-9937
Kit includes: PEEK capillary, 0.13 mm ID, 1.5 m Qty 4 Stainless steel capillary, 105 x 0.12 mm Qty 4 Stainless steel capillary, 150 x 0.12 mm Qty 2 Stainless steel capillary, 200 x 0.12 mm Qty 2 Stainless steel capillary, 280 x 0.12 mm Stainless steel capillary, 400 x 0.12 mm Tubing cutter for PEEK capillaries 1/16 in. stainless steel fittings, 10/pk 1/16 in. PEEK fittings, color, 10/pk 1/16 in. PEEK fittings, 10/pk Qty 3 ZDV fitting Rheotool Cybertool
Capillary/fitting starter kit for 1100 Capillary LC System
5065-9938 Kit includes: Qty 2 Fused silica/PEEK capillary, 50 m, 55 cm Qty 1 Fused silica/PEEK capillary, 50 m, 20 cm Qty 1 Fused silica/PEEK capillary, 100 m, 110 cm Qty 2 Fused silica/PEEK capillary, 50 m, 50 cm Qty 2 Fused silica/PEEK capillary, 50 m, 40 cm Qty 4 4 mm stainless steel fitting, male 10-32 Qty 4 1/32 in. PEEK ferrule and stainless steel lock ring Qty 4 PEEK fittings for -valves Qty 4 Double winged PEEK nuts and 1/32 in. ferrules Qty 1 Cybertool Kit includes: Qty 5 Capillaries to optimize 1100 Series LC for use with RRHT columns Includes capillaries to optimize 1200 LC for low dispersion operation 5065-9947
Rapid Resolution High Throughput capillary kit 1200 capillary kit for 0.12 mm ID
G1316-68716
92
General Supplies
HPLC In-Line Filters

Description RRLC in-line filter 4.6 mm, 0.2 m pore size filter, connecting capillary, max 600 bar RRLC in-line filter 2.1 mm, 0.2 m pore size filter, connecting capillary, max 600 bar Low dispersion in-line filter Includes two frits, 2.1 mm, 2 m pore size; filter holder with inserts; 60 x 0.12 mm connecting capillary Universal in-line filter Includes two frits, 4.8 mm, 2 m pore size; filter holder with inserts; 130 x 0.25 mm connecting capillary Semi prep filter High pressure semi prep filter Prep filter In-line filter for G1311A 1290 in-line filter Frit Porosity (m) 0.2 Frit Inlet ID (mm) 4.6 Comments max 600 bar Part No. 5067-1553 Replacement Frits 5067-1562, 10/pk
0.2
2.1
max 600 bar
5067-1551
5067-1555, 10/pk
2 0.5
2.1
< 1 mL/min
01090-68702 280959-904, 10/pk 280959-907, 10/pk
4.8
1-5 mL/min
01090-68703 01090-27609, 2/pk
0.5 10 10
12.7 19
1-5 mL/min 5-10 mL/min
5064-8273 5022-2165
5022-2185 5022-2166, 10/pk 5065-9901 Replacement Glass Cartridge
10-100 mL/min 5065-4500 G1311-60006 5067-4638
Recommended when high salt concentrations are used 0.3 2.0 1200 bar
5023-0271, 5/pk
93
General Supplies
Solvent Filters
Description Glass filter, solvent inlet, 20 m pore size Glass filter, solvent inlet, 40 m pore size Glass filter, solvent inlet, 40 m pore size Recommended Use Analytical scale, micro scale Preparative LC For G2258A Dual Loop Autosampler Part No. 5041-2168 3150-0944 3150-0944 Frit Adapter Part No. Frit Inlet Frit Inlet ID (mm) ID (mm) 5 7 7 3.2 4 3.2 Frit adapter, PTFE, 3 mm, 4/pk 5062-8517 Frit adapter, 4 mm, PTFE Frit adapter, PTFE for 1/8 in. OD tubing G1361-23204 G2258-23201
Frits and Adapters

Description Solvent mixer, 1100 Series Solvent mixer, short, 200 L Frit adapter, PTFE, for 4.7 mm OD tubing Frit adapter, PTFE, for 1/8 in. OD tubing For G2258A Dual Loop Autosampler O-ring, Viton, 30 mm Stainless steel filter assembly with PEEK ring, 2 m pore size
Glass filter, 5041-2168
Part No. G1312-87330 5067-1565 G1361-23205 G2258-23201 0905-1516 5022-2192
94
General Supplies
Sample Filtration
Syringe Filters, Membrane Econofilters, 200/pk
Description Regenerated cellulose Regenerated cellulose
Membrane Econofilters, 5185-5830
Diameter (mm) 25 25 25 25 25 25
Pore Size (m) 0.2 0.45 0.2 0.45 0.2 0.45
Part No. 5185-5830 5185-5831 5185-5832 5185-5833 5185-5834 5185-5835
Nylon Nylon PTFE PTFE
Mini-UniPrep Syringeless Filters, 100/pk

Description 0.45 m PTFE For aggressive samples 0.45 m Nylon For aqueous and organic samples, pH 3-10
Mini-UniPrep Syringeless Filters
Part No. 5190-1415 5190-1416 5190-1417 5190-1418 5190-1419 5190-1420 5190-1421 5190-1422
0.45 m PP For solvent based samples, low water breakthrough values 0.45 m regenerated cellulose For aqueous or organic solvents, very low non-specific protein binding membrane 0.20 m PTFE For aggressive samples 0.20 m Nylon For aqueous and organic samples, pH 3-10 0.20 m PP For solvent-based samples, low water breakthrough values 0.20 m regenerated cellulose For aqueous or organic solvents, very low non-specific protein binding membrane
For more information, including chemical compatibilities, visit www.agilent.com/chem/miniuniprep
95
General Supplies
Solvent Filters/Degassers
Description HPLC solvent filter/degasser assembly
Replacement Parts for 3150-0577
Part No. 3150-0577 5188-2743 5188-2744 5188-2745 5188-2746 3150-0576 9301-0895 3150-0509
Glass funnel, 250 mL PTFE coated sieve PTFE seal Funnel base, glass
Filter Membranes
Regenerated Cellulose filter membranes Diameter 47 mm, pore size 0.45 m, 100/pk Nylon filter membranes Diameter 47 mm, pore size 0.45 m, 100/pk PTFE filter membranes Diameter 47 mm, pore size 0.45 m, 10/pk
96
Agilent e-Seminars
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With different series and topics for troubleshooting, maintenance, informatics, compliance, and industry applications, Agilent e-Seminars are the easy way to get the latest information without the hassle or expense of travel. These free 60 to 90 minute online seminars are offered continuously:
Choose from live or recorded events to best fit your schedule Participate wherever you are from the office, home, or hotel Ask Agilent experts and industry leaders questions during live Q&A sessions
For more information and to see a complete listing of available sessions, visit www.agilent.com/chem/eseminars
How to contact Agilent

For the latest information on Agilent products and services: Visit our Web site: www.agilent.com/chem Contact your local Agilent sales office Contact your local Agilent Authorized Distributor
Information, descriptions, and specifications in this publication are subject to change without notice. Agilent Technologies, Inc. 2009 Printed in the USA November 9, 2009 5990-4957EN

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Maintaining Your Agilent LC and LC/MS Systems

Our measure is your success.

Agilent Systems for UHPLC and HPLC.............1 Pump Maintenance..........................................9

Autosampler Maintenance ............................27

Fraction Collector Maintenance ....................39

Valve Maintenance ........................................45

LC/MS System Maintenance ........................69

Welcome to the next generation of HPLC

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Agilent Systems for UHPLC and HPLC

Agilent 1290 Infinity LC System

ZORBAX Rapid Resolution High Definition (RRHD) 1.8 m Columns

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Agilent Systems for UHPLC and HPLC

Widest power range for infinitely better performance and flexibility

Agilent 1290 Infinity LC System

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Agilent Systems for UHPLC and HPLC

Agilent 1200 Series Rapid Resolution LC System

Get the maximum in speed, resolution, sensitivity and flexibility

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Agilent Systems for UHPLC and HPLC

Agilent 1200 Series Rapid Resolution LC System

Agilent ZORBAX Rapid Resolution High Throughput Columns

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Agilent Systems for UHPLC and HPLC

Agilent 1200 Series Standard LC Systems

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1200 Series Binary LC System

Agilent 1200 Series Standard LC Systems

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Agilent Systems for UHPLC and HPLC

Agilent Rack for LC Systems

Agilent rack for LC system, 5001-3726

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Pump Maintenance Tips

Scratch on plunger Leaky wash seals

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Wrench 1/4-5/16 inch Wrench 14 mm Wrench 1/4 inch Pair of tweezers

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Replacing the Active Inlet Valve Cartridge

Wrench 14 mm Pair of tweezers

Active inlet valve body

Active inlet valve

Solvent inlet tube

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Cleaning the Solvent Filter

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Exchanging the Outlet Ball Valve

Wrench 1/4 inch Wrench 14 mm Pair of tweezers

Sieve (G1312A/B only) Gold seal

Outlet ball valve

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Pistons and Seals

lasting pistons in the world

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