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Lab Activity 1: Determination of Copper Concentration in a Wastewater Sample Group no. Group members (name and i.d.) (1) (2) (3) (4)
2. Objectives
(1) To perform a calibration experiment using standard solutions. (2) To determine the concentrations of copper found in wastewater samples. (3) To investigate the effect of instrument condition variations on the readings produced.
3. Materials
standard solution of copper in deionised water simulated wastewater samples of copper chloride dihydrate in deionised water graph papers
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4. Experimental Procedure:
4.1 Standard solution preparation
(1) A stock copper ion solution is provided, prepared by dissolving pure copper ion solution in deionized water. (2) 5 sets of standard solutions are prepared from the stock solution, labelled as AF accordingly. (3) Perform calibration experiment using these samples, list the corresponding concentrations in section 5 (Results and Discussion).
4.2
(1) Identify the main components involved: a. Fuel gas b. Oxidant c. Instrument d. Blower e. Lamp f. Flame location (2) Close the air release valve of the compressor. (3) Turn on the acetylene gas by turning the knob anti-clockwise. (4) Switch on the power supply for air compressor. (5) Switch on the AAS instrument. (6) Turn on the blower (to remain on during the course of experiment). (7) Open the software WinLab32 a. Window will show system status wait till green light comes on. (8) Create work station. a. Flame b. Manual analysis control c. Lamp set up choose lamp (9) Create a new method. a. File New Method b. Starting condition c. Element choose element, then click OK d. Method Editor define element i. Spectrometer do not change ii. Sampler do not change iii. Calibration iv. File save as name (10) To start, go to: open method lamp station (11) Flame control: on (12) Manual analysis control: analyse blank (13) Insert sampling tube into Standard 1 click analyse standard a. When result appears, remove tube, re-insert into water to rinse sample residue. (14) Repeat step (13) until all samples are analysed. (15) To stop the experiment: a. Turn off flame b. Turn off lamp c. Switch off instrument d. Turn off acetylene and compressor e. Release air from compressor.
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5.
H J K L M *Note: the samples used in the AAS analyses have been diluted. You need to determine the actual concentrations based on the dilution factor used.
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Comment on linearity:
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Comment on the effects of instrument condition variation to the results produced. (1) Wavelength
How can the sensitivity be adjusted? What are your recommendations to achieve the most efficient analysis?
Calculations:
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