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  • Methods For The Study of Deep-Sea Sediments, Their Functíoning and Biodíversity

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    David Masalles
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    This document is a book that provides methods for studying deep-sea sediments, biodiversity, and ecosystem functioning. It contains 42 chapters organized into 6 sections that cover topics such as measuring environmental variables in sediments, quantifying the food supply to deep-sea floors, assessing abundance and diversity of different benthic organisms, conducting deep-sea experiments, and analyzing ecosystem functions like organic matter degradation. The book serves as a reference for standard techniques to characterize deep-sea sediment environments and the living communities that inhabit them.

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    Methods for the Study of Deep-Sea Sediments, Their Functíoning and Biodíversity

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    This document is a book that provides methods for studying deep-sea sediments, biodiversity, and ecosystem functioning. It contains 42 chapters organized into 6 sections that cover topics such as measuring environmental variables in sediments, quantifying the food supply to deep-sea floors, assessing abundance and diversity of different benthic organisms, conducting deep-sea experiments, and analyzing ecosystem functions like organic matter degradation. The book serves as a reference for standard techniques to characterize deep-sea sediment environments and the living communities that inhabit them.

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    Attribution Non-Commercial (BY-NC)
    Baixe no formato PDF, TXT ou leia online no Scribd
    Sinalizar o conteúdo como inadequado
    155 visualizações5 páginas

    Methods For The Study of Deep-Sea Sediments, Their Functíoning and Biodíversity

    Enviado por

    David Masalles
    This document is a book that provides methods for studying deep-sea sediments, biodiversity, and ecosystem functioning. It contains 42 chapters organized into 6 sections that cover topics such as measuring environmental variables in sediments, quantifying the food supply to deep-sea floors, assessing abundance and diversity of different benthic organisms, conducting deep-sea experiments, and analyzing ecosystem functions like organic matter degradation. The book serves as a reference for standard techniques to characterize deep-sea sediment environments and the living communities that inhabit them.

    Direitos autorais:

    Attribution Non-Commercial (BY-NC)
    Baixe no formato PDF, TXT ou leia online no Scribd
    Sinalizar o conteúdo como inadequado
    de 5

    Methods for the Study of Deep-Sea Sediments, Their Functoning and Biodversity

    Edited by

    Roberto Danovaro

    CRC Press
    Taylor & Francis Group

    Contents
    Foreword Premise Preface Conributors Introduction xi xiii xv xvii xix

    ."

    SECTION I
    Chapter 1

    Deep-Sea Environmental Variables


    3

    Total Organic Matter and Water Contents, Grain Size, Bulk Density, Porosity, and Redox Potential of Sediments

    Chapter 2

    Total Organic Carbn, Total Nitrogen, and Organic Phosphorus in Marine Sediments ,

    13

    Chapter 3

    Bioavailable Organic Matter: Total and Enzymatically Hydrolyzable Proteins, Carbohydrates, and Lipids

    23

    Chapter 4

    Photosynthetic Pigment Concentrations in Marine Sediments

    45

    SECTION II

    Food Supply of Organic Matter to the Deep-Sea Floor


    55

    Chapter 5

    Fluxes of Labile Organic Matter to the Sea Floor

    Chapter 6

    Total Carbohydrate Flux from Sediment Trap Samples

    59

    Chapter 7

    Total Protein Flux from Sediment Trap Samples

    63

    Chapter 8

    Total Lipid Flux from Sediment Trap Samples

    69

    Chapter 9

    Total DNA from Sediment Trap Samples

    75

    Chapter 10 Phytopigment Flux from Sediment Trap Samples

    81

    viii

    Contents

    SECTION III

    Deep-Sea Benthic Life


    87

    Chapter 11 Viral Abundance

    Chapter 12 Prokaryotic Abundance

    95

    Chapter 13 Fluorescence In Situ Hybridization and Catalyzed Reprter Deposition for Benthic Prokaryote Assemblage Structure

    107

    Chapter 14 Prokaryotic Abundance by Real-Time PCR

    121

    Chapter 15 Abundance of Heterotrophic Benthic Protists

    131

    Chapter 16 Abundance of Benthic Foraminifera

    141

    Chapter 17 Abundance of Metazoan Meiofauna

    149

    Chapter 18 Macrofaunal Abundance

    161

    Chapter 19 Megafaunal Abundance

    167

    SECTION IV

    Deep-Sea Benthic Diversity


    181

    Chapter 20 Extraction and Purification of DNA from Marine Sediments Suitable for the Analysis of Prokaryotic Diversity

    Chapter 21 Archaeal Diversity Analysis Using 16S rDNA T-RFLP (Termimil-Restriction Fragment Length Polymorphisms)

    197

    Chapter 22 Benthic Bacterial Diversity Based on Cloning and Sequencing of 16S rRNA Genes

    209

    Chapter 23 Benthic Bacterial Diversity Analysis Based on ARISA (Automated Ribosomal Intergenic Spacer Analysis)

    223

    Chapter 24 Meiofaunal Diversity

    235

    Contents Chapter 25 Macrofaunal and Megafaunal Diversity

    ix 253

    Chapter 26 Parameters for the Measurement of Marine Benthic Structural and Functional Diversity

    259

    SECTION V

    Deep-Sea Benthic Functioning


    269

    Chapter 27 Deep-Sea Experiments and Manipulations

    Chapter 28 Effect of Pressure on Enzymatic Activities, Viral Production, and Prokaryotic Heterotrophic Production

    285

    Chapter 29 Organic Carbn Remineralization Rates in Marine Surface Sediments

    291

    Chapter 30 Degradation and Turnover of Organic Matter in Marine Sediments

    297

    Chapter 31 Extracellular DNA Extraction from Marine Sediments

    303

    Chapter 32 Degradation of Extracellular DNA in Marine Sediments

    311

    Chapter 33 Viral Production in Marine Sediments

    317

    Chapter 34 Determination of Living/Dead and Active/Dormant Bacteria! Fractions in Marine Sediments

    323

    Chapter 35 Prokaryotic Biomass in Marine Sediments

    331

    Chapter 36 Benthic Prokaryotic Heterotrophic Production Using the Leucine Incorporation Method

    337

    Chapter 37 Prokaryotic Chemoautotrophic Production in Marine Sediments

    343

    Chapter 38 Meiofaunal Biomass and Secondary Production

    351

    Chapter 39 Macrofaunal and Megafaunal Biomass and Biochemical Composition

    369

    Chapter 40 Protistan Grazing on Benthic Prokaryotes and Viruses

    377

    x Chapter 41 Meiobenthic Predation on Prokaryotes Chapter 42 Analysis of Benthic Food Webs and Benthic Trophodynamics

    Contents 385 393

    SECTION VI Suggested Readings for the Study of Deep-Sea Biodiversity and Ecosystem Functoning
    Suggested Readings Index 405 417

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