LTPC: 3 0 3 4 Immunology and Immuno-technology 1.0.

0 Biochemistry, Animal Physiology and Biotechnology To introduce the concept of immune response in a mammalian host thereby emphasize their significance in innovation in developing therapeutic modalities for immunological disorders of humans, to orient the students to the biology of the immune system Expected Outcome: The course outlines the basis of immune responses. Also it describes the effector molecules that mediate the functions, the major signaling pathways of activation of cells of the immune system, the abnormal responses that result in disease Unit No. 1 Immune system Number of hours: 8 An overview of the immunology Cells and tissues of the immune system- Haematopoiesis: Origin and differentiation of Lymphocytes and phagocytic cells- receptors and signals that control lymphocyte lineage commitment. Immunogens and antigens, Innate and acquired immunity. Elements of Immunity B lymphocytes and thymus derived (T) Lymphocytes. Unit No. 2 Humoral and Cellular Immunity Number of hours: 9 Immunoglobulins - Classes and subclasses, organization and expression of immnunoglobulin genes. immunoglobulin gene rearrangement antibody diversity Clonal theory: B cell memory and immune response. Classification of thymus derived (T) Lymphocytes, apoptosis, T cell receptor gene rearrangement, antigen presenting cells. Macrophages, Langerhans cells, dendritic cells, mechanism of phagocytosis, molecular structure and assembly of MHC molecules, Classes of MHC MHC/HLA genetic loci , antigen processing and presentation. Unit No. 3 Immunity to infection and Number of hours: 9 Hypersensitivity Reactions An overview of immune response to infections, complement systems, Hypersensitivity reactions, case studies with remedial measures; cytokines, Immunological tolerance- role of cytokines and regulatory and immunosuppressive T cells- role of B cells in oral tolerance- T cell tolerance-idiotype and anti-idiotype network theory Unit No. 5 Immunology of tumors, autoimmunity and Number of hours: 9 transplantation Transplantation: Types, immunological mechanisms of graft rejection- immunological strategies to prevent graft rejection-role of immunosuppressive drugs. Autoimmunity: HLA alleles and disease susceptibility- an overview of the immunopathogenic mechanisms of autoimmunity -therapeutic approaches. Tumors: Immune response to tumors-types of tumor antigens- immunological approaches to tumor regression. Unit No. 5 Immunotechnology Number of hours: 9 Hybridoma technology - Production and purification of monoclonal antibodies and their application in clinical Diagnosis. Antibody engineering: definition; applications. Immuno diffusion & immuno electrophoresis, ELISA, ELISpot, Immuno fluorescence. Application of Antibodies: Western Blotting; Flow Cytometry; Antibody Arrays; Immunostaining; Immunoblotting; Immunoassay; Immunoaffinity Chromatography; Catalytic Antibodies and phage display. No. Experiment Complement mediated lysis of sheep RBC coated with amboceptor (antisheep RBC antibody) 1. Version No. Course Prerequisites Objectives: Immunodiffusion single radial diffusion 2. Immunodiffusion double diffusion 3. Latex Agglutination test 4. 5. VIDAL tube agglutination test

Quantitative precipitin assay 6. Immuno-electrophoresis & Immunoblotting 7. Immunostaining 8. Antibody labeling with enzymes 9. ELISA 10. Text Books Reference s 1. Goldsby. R.A, Kindt.T.J, Kuby J and Osborne BA Immunology, 5th Eds. W.H. Freeman Pubn. 2002. 2. Abbas K A, Litchman A. H. Cellular and Molecular Immunology. 5th Edn., W. B. Saunders Co. 2005. 1. Roitt I.M., Brostoff. J, Male. D.K. Immunology, 5th B. K. & Cdr eds, Garland Pub Ltd. 2001. 2. C. A. Janeway Jr, P. Travers. Immunobiology, 4th Ed. (or later). 3. R.M. Coleman, M.F. Lombard, R.E.Sichard. Fundamental Immunology, 2nd Ed (or later)

Mode of Written Examinations/ Assignments/ Seminars Evaluation Recomme 13th November, 2008 nded by the Board of Studies on Date of Approval by the Academic Council