Tp ch Khoa hc 2009:11 33-40

Trng i hc Cn Th

NH HNG THUC TR SU CHA HOT CHT DIAZINON LN HOT TNH ENZYME CHOLINESTERASE C R NG (ANABAS TESTUDINEUS): HIU NG CA NHIT V OXY HA TAN
Ng T Linh v Nguyn Vn Cng1

ABSTRACT
Pesticide uses for rice increased greatly in the Mekong delta. Although spraying pesticides is one of common ways to protect rice from insects, it also causes negative effects. Climbing perch (Anabas testudineus) is often found on ricefields; therefore, fish could not avoid of exposure to pesticides. Dissolved oxygen (DO) and water temperature (WT) on ricefields are highly fluctuated. Three levels of WT (20, 25 & 30oC) and two levels of DO (<2 & >5mg/L) were conducted in laboratory to assess effects of diazinon on brain and muscle cholinesterase activity of the perch. Results indicate that WT, DO and interaction between WT and DO did not affect on brain and muscle ChE in the control fish. Increasing WT leaded to more ChE inhibition in diazinon treatment; DO and interaction between DO and WT did not affect on ChE inhibition by diazinon. The study suggests that the species is more risk of ChE inhibition in the high temperature. Keywords: Diazinon, Cholinesterase, Anabas testudineus, Temperature, Dissolved oxygen Title: Effects of insecticide diazinon on activity of enzyme cholinesterase in Climbing perch (Anabas testudineus): Effects of temperature and dissolved oxygen

TM TT
Phun thuc su trong cho la gia tng ng k ng bng sng Cu Long. D phun thuc l bin php tt bo v la nhng n cng gy hi cho mi trng. C r ng (Anabas testudineus) thng xut hin trn rung nn kh trnh khi tip xc vi thuc su. Oxy ha tan (DO) v nhit nc (WT) rung dao ng ln trong ngy v tui la. Ba mc WT (20, 25 v 30oC) v hai mc DO (<2 v >5mg/L) c b tr theo khi hon ton ngu nhin trong phng th nghim tm hiu nh hng ca n n cholinesterase (ChE) trong no v tht c khi tip xc vi diazinon. Kt qu cho thy WT v DO khng nh hng n ChE trong no v tht c i chng. Khi c diazinon, WT lm tng c ch ChE trong no v tht. DO v tng tc gia DO v WT khng nh hng n c tnh ca diazinon n c. Kt qu cho thy c r c nhiu ri ro ChE b c ch khi phun thuc nhit cao. T kha: Diazinon, nhit , oxy

Khoa Mi trng v Ti nguyn thin nhin, Trng i hc Cn Th

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