WILFRID LAURIER UNIVERSITY

R ES E ARC H I N S T RU M E NTAT I ON H A N DB O OK
POLICIES AND FEES FOR INSTRUMEN T USE

E F F E C T I V E : M AY 1 , 2 0 1 0 A P R I L 3 0 , 2 0 1 1 REVISION: 1

RESEARCH INSTRUMENTATION HANDBOOK

TA BLE O F CON TE NTS

GENERAL USER GUIDELINES
LABORATORY SAFETY 2

MULTI-USER INSTRUMENTS AMINCO/THERMO FRENCH PRESS BECKMAN AVANTI J30I CENTRIFUGE CARY 50 UV-VIS SPECTROMETER CARY ECLSIPSE FLUORESCENCE SPECTROMETER CENTRIVAP CENTRIFUGAL CONCENTRATOR DNR BIS 303 IMAGING SYSTEM DYNEX MRX REVELATION PLATE READER EPPENDORF 5804R REFRIDGERATED CENTRIFUGE GLASSCOUNTOUR DRY SOLVENT SYSTEM INSTA-ICE 475 DRY ICE BLOCK MAKER MIELE GLASSWARE WASHER MILLI-Q SYNTHESIS A-10 STERIS PREVACUUM STERILIZER ULTRALOW -80 FREEZERS SPECIALIZED INSTRUMENTS
CHROMATOGRAPHY TITRATION SPECTROSCOPY DNA ANALYSIS MICROSCOPY OTHER SPECIALIZED EQUIPMENT

3 3 3 4 4 5 5 5 5 6 6 6 7 7 7 8 8 9 9 10 12 13

TRAINING AND USER FEES

USER TRAINING AND CERTIFICATION 15

INTERNAL USER FEES

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EXTERNAL USER FEES

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TECHNICAL PROBLEMS AND EQUIPMENT DAMAGE

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RESEARCH INSTRUMENTATION HANDBOOK

PURPOSE
This Instrumentation Handbook has been developed to promote efficient and safe instrument operation and facilitate research collaboration within the Faculty of Science. This handbook lists the instruments currently available for research use and describes the policies and fees governing the use of these instruments. Some of the instruments listed are owned by the Faculty of Science, and are referred to as multi-user instruments. The other instruments listed, termed specialized instruments, are controlled by specific researchers, and are available to other users at the discretion of the researcher.

GENERAL USER GUIDELINES

LABORATORY SAFETY Comprehensive guidelines for laboratory safety,1 biosafety,2 and radiation safety3 have been developed by the Environmental/Occupational Health and Safety Office, and are available on-line. Specific guidelines for the safe use of each multi-user instrument are provided in the User Guidelines and Standard Operating Procedure (SOP) for that specific instrument. The SOPs are also available on-line.4 In all cases, individuals using any laboratory instrument must be aware of the principles of operation, associated hazards, personal protective equipment, and related waste disposal before commencing use. The Instrumentation Technician provides training for many of the instruments listed in this handbook (see the Training section for further details). Laboratory cleanliness must be maintained by everyone using a multi-user or specialized instrument. The laboratory space around the instrument must remain free of dust, debris, and personal items. Each user is responsible for cleaning the area around an instrument after use.

1 2

Laboratory Health and Safety Manual: www.wlu.ca/documents/23120/Laboratory_Health_%26_Safety_Manual.pdf Biosafety Manual: www.wlu.ca/documents/23632/Biosafety_Manual_March_15_2007_Final.pdf 3 Radiation Safety Manual: www.wlu.ca/documents/14021/RSM_Jan_2010.pdf 4 Link for instrument SOPs: www.wlu.ca/page.php?grp_id=2106&p=10255

RESEARCH INSTRUMENTATION HANDBOOK MULTI-USER INSTRUMENTS

The multi-user instruments purchased by the Faculty of Science are available to all internal users, and to external users as determined by the Instrumentation Technician. Specialized instruments purchased under individual faculty grants are available to internal and external users at the discretion of the researcher (referred to as the principal investigator or PI). All of the multi-user instruments listed in this section are available for use on a first come, first serve basis, except the fluorescence spectrometer. Twenty-four hours notice is required to use the fluorescence spectrometer because some samples are time sensitive, and may take up to 8 hours to analyze. A sign-up sheet is available in the fluorescence spectrometer log book. Data recording and back-up is the sole responsibility of the user. Data should be transferred to another computer for safe-keeping, rather than stored on the computer that controls a multi-user instrument. AMINCO/THERMO FRENCH PRESS PI: Dr. Art Szabo The French Press can be used for cell disruption or subcellular fractionation, enzyme preparation, bacterial and plant tissue degradation, and recovery of intercellular proteins and nucleic acid. The cell operates at pressures up to 40,000 psi and can contain a maximum volume of 35 mL.

BECKMAN AVANTI J30I CENTRIFUGE PI: Dr. Art Szabo/Dr. Matt Smith/Science The Avanti J-30I centrifuge can be used to achieve very fast centrifugal separations. Swinging-bucket and fixed-angle rotors provide maximum separation forces in excess of 100,000 x g at speeds up to 30,000 RPM. There are three different rotors that may be used in this centrifuge: the JLA10.500 fixed angle rotor (holds 6 x 500 mL flat bottomed vessels); the JA-30.50 Ti fixed angle rotor (holds 8 x 50 mL round bottomed tubes); and the JS 13.1 swinging bucket rotor.

RESEARCH INSTRUMENTATION HANDBOOK CARY 50 UV-VIS SPECTROMETER PI: Dr. Art Szabo The Cary 50 can be used to measure the absorbance / transmittance / optical density of a variety of samples using the following software applications: Advanced Reads: Read multiple samples in a single run. Concentration: Quantitative analysis using a calibration curve. Color: Measure and calculate transmitted or reflected sample colour. Enzyme Kinetics: Kinetics analysis using Michaelis-Menten equation. Kinetics: Time-based single or multicell measurements. RNA / DNA: Asses the amount, type and purity of nucleic acid. Scan: Measure absorbance across a range of wavelengths. Scanning Kinetics: Determine kinetics curves from a set of spectra. Simple Reads: Measure absorbance readings at a single wavelength.

Thermal: Absorbance measurements with temperature variation, often used for DNA melts.

CARY ECLIPSE FLUORESCENCE SPECTROMETER PI: Dr. Art Szabo The Cary Eclipse can be used to measure sample fluorescence, phosphorescence, or bioluminescence using the following software applications: Advanced Reads: Read multiple samples in a single run. Concentration: Perform quantitative analysis with a calibration curve. Kinetics: Measure the change in emission intensity over time. Lifetimes: Measure phosphorescence lifetimes or delayedfluorescence lifetimes. Scan: scan the emission or excitation spectrum of samples across a wavelength or wavenumber range. Simple Reads: Measure a sample at a single wavelength Thermal: Perform thermal analyses on DNA using the 4-position multicell Peltier accessory.

RESEARCH INSTRUMENTATION HANDBOOK CENTRIVAP CENTRIFUGAL CONCENTRATOR PI: Dr. Art Szabo The Centrivap centrifugal concentrator uses centrifugal force, heat, and vacuum to remove water from multiple small samples. The rotor can accommodate up to 132 x 1.5 mL samples in Eppendorf tubes, 18 x 15 mL samples, or 12 x 50 mL samples. Solvents cannot be removed using this system.

DNR BIS 303 IMAGING SYSTEM PI: Faculty of Science The BIS 303 PC is a bio-imaging system used to image Chemiluminescence on agarose and polyacrylamide gels, membranes, films, blots, TLC plates, and autoradiographs. Gels and plates of up to 12 x 25 cm can be imaged using a cooled 1.75 MP CCD camera connected to the external PC. The final images can be saved onto a disk or memory key.

DYNEX MRX

REVELATION PLATE READER PI: Faculty of Science The MRX Revelation reads sample optical density in a 96-well plate and can be used for endpoint, kinetic and tissue culture agglutination assays. This instrument has a spectral range of 405 to 850 nm Up to six filters can be used in single or dual wavelength reading modes.

EPPENDORF 5804R REFRIDGERATED CENTRIFUGE PI: Faculty of Science This refrigerated benchtop centrifuge is equipped with a swinging bucket A-4-44 rotor and adaptors which can accommodate 2 mL and 15 mL tubes and operate at speeds up to 5000 RPM. The temperature can be set between -9 oC to 40 oC, and the centrifuge can remain in standby to maintain temperature when not in use. There are ten different acceleration and deceleration rates for sensitive samples, and up to 34 individual user settings can be programmed.

RESEARCH INSTRUMENTATION HANDBOOK GLASSCOUNTOUR DRY SOLVENT SYSTEM PI: Faculty of Science

The dry solvent system provides a safe alternative to solvent distillation, and produces dry high purity solvents on demand. Solvents pass through activated columns under low pressure to remove trace impurities and water. The dry solvents currently available are tetrahydrofuran, ether, dichloromethane, toluene, hexane, acetonitrile, dimethylformamide, and methanol.

INSTA-ICE 475 DRY ICE BLOCK MAKER PI: Faculty of Science The 475 Ice block maker can be used to produce small dry ice blocks, up to 8.5 x 6.7 x 18.4 cm. Dry ice is often used for organic chemistry applications or shipment of sensitive samples.

MIELE GLASSWARE WASHER PI: Faculty of Science The Miele general purpose laboratory washer is equipped with three wash routines, and can accommodate a variety of baskets and inserts specifically designed for laboratory glassware. An injection basket is available for narrow mouth glassware and standard baskets should be used for wide mouthed items. The wash and DI rinse water are heated to 70C to ensure proper removal of both the contaminant and detergent residues.

RESEARCH INSTRUMENTATION HANDBOOK MILLI-Q SYNTHESIS A-10 PI: Faculty of Science The Milli-Q A-10 Synthesis provides high quality ultrapure water for a variety of sensitive applications. Milli-Q water has a resistivity of 18.2 M.cm @ 25C, and less than 10 ppb TOC. The main components of the Milli-Q system are as follows: Q-Gard purification pack for the removal of organics and ions from the building DI feedwater. Quantum EX cartridge for additional organic removal to trace levels using mixed acid and base resins and activated carbon. Millipak 0.22 m membrane filter. UV lamp to destroy organic molecules and reduce TOC levels to < 10 ppb. Pyrogard 5000 ultrafiltration cartridge for the production of pyrogenand nuclease-free ultrapure water (< 0.001 EU/mL), which is particularly important for life science or molecular biology applications.

STERIS PREVACUUM STERILIZER PI: Faculty of Science This autoclave is designed for fast, efficient sterilization of heat- and moisture-stable materials including media, glassware, biological waste, and soils. Various liquid, gravity, and prevacuum cycles are available. The autoclave is tested regularly by the Instrumentation Technician to ensure proper operation. Users are responsible for testing their own sterilization procedures, particularly with regard to waste.

ULTRA-LOW -80 FREEZERS PI: Dr. Matt Smith, Dr, Lucy Lee, Dr. Art Szabo There are several ultra-low freezers in the Science Research building. These freezers can only be used after consultation with the appropriate PI. The samples in these freezers are very sensitive and valuable, and brief training is therefore required before using any of the ultra-low freezers.

RESEARCH INSTRUMENTATION HANDBOOK SPECIALIZED INSTRUMENTS A wide variety of specialized and complex equipment is present within the Faculty of Science. Some of the specialized instruments purchased under individual faculty grants are available to internal and external users at the discretion of the PI. If this use is not part of an official collaboration, user and training fees may apply. Users are also responsible for the costs of consumables, as determined by the PI. The following instruments are considered specialized equipment:

CHROMATOGRAPHY VARIAN SATURN 2000 GC/MS A GC/MS can be used to separate a wide variety of compounds based on volatility and polarity, depending on the column used. This technique is particularly useful for small quantity samples. The analyte of interest must be volatile and stable at high temperatures (i.e. up to ~350 oC). The Varian GC-MS is equipped with a CP8200 autosampler which can hold up to 48 vials, and allows for liquid, solid phase microextraction (SPME), or ambient headspace injection of volumes using a 10 l or 100 l syringe. The 1079 programmable temperature vaporizing (PVT) injector can be used for split and splitless, temperature-ramped splitless, large volume (LVI), and cold oncolumn injection. Fractions exiting the column can be detected using flame ionization (FID), electron capture (ECD), and/or mass spectrometry. PIs: Dr. Frdrique Guinel, Dr. Mary Ann Fieldes WATERS DELTA 600 HPLC (2) There are two Waters Delta HPLC systems in the Faculty of Science. Combined, these systems offer semi-preparative and analytical capabilities; a photodiode array; absorbance, or refractive index detection; an autosampler; and fraction collection. Separations can be run in both isocratic and gradient mode at flow rates up to 20 ml/min depending on the column. Waters Empower software can be used to control runs and analyze data. PIs: Dr. Masoud Jelokhani-Niaraki, Dr. Steve MacNeil WATERS MICROMASS ZQ LC-MS This HPLC-MS system is equipped with two Waters 515 pumps, an absorbance detector, a column oven, and the Micromass ZQ single quadrupole mass spectrometer. The ZQ can ionize samples using either electrospray ionization (+/- ESI) or atmospheric pressure chemical ionization (APCI). Data collection and analysis are conducted using Masslynx 4.0 software. PI: Dr. Masoud Jelokhani-Niaraki

RESEARCH INSTRUMENTATION HANDBOOK TITRATION METTLER TOLEDO DL32 KARL FISHER TITRATOR

The DL32 is a coulometric KF titrator which can be used to determine water contents in small sample volumes. The DL32 is ideal for water contents in the range of 1 ppm to 5%. PI: Dr. Steve MacNeil MICROCAL VP-ITC ISOTHERMAL TITRATOR MICROCALORIMETER The VP-ITC can be used to study reaction thermodynamics, binding constants, and stoichiometry. This instrument measures the heat released or absorbed as a reaction occurs, and can be used to monitor reactions that are initiated by a binding component, including: antigen-antibody, DNA-drug, and protein-ligand reactions. Typical injection volumes are between 2-10 L, and sample/macromolecule concentrations should be between 5 M 1 mM. PI: Dr. Masoud Jelokhani-Niaraki TANAGER DUAL PH METER AND TITRIMETER This automatic titrator is interfaced with a modular spectrometer system and can be used to run controlled reactions while monitoring a variety of physical and chemical parameters. PI: Dr. Scott Smith

SPECTROSCOPY AVIV CIRCULAR DICHROSIM SPECTROMETER The circular dichroism (CD) spectrometer can be used to assess the conformation of various sample types, including DNA and proteins. This instrument can be used to conduct wavelength, temperature, kinetic, and titration experiments. CD spectra can be recorded as a function of wavelength, time, temperature, pH, or concentration. PI: Dr. Masoud Jelokhani-Niaraki MODULAR FIBER OPTIC SPECTROMETER This modular fiber optic system includes a 1m light path for colorimetery, a UV-Vis absorbance fiber optic probe, and a fluorescence fiber optic probe. The spectrometer components are connected to a Tanager autotitration system (see above). PI: Dr. Scott Smith MOLECULAR DEVICES SPECTRAMAX M2 MICROPLATE SPECTROPHOTOMETER This microplate reader is equipped with dual-monochromators and dual-mode cuvette ports for absorbance and fluorescence intensity detection. Endpoint, kinetic, spectrum, and area-well scanning assays can be conducted on 6- to 384-well microplates. PI: Dr. Jim McGeer

RESEARCH INSTRUMENTATION HANDBOOK VARIAN SPECTRA 880 ATOMIC ABSORPTION SPECTROMETER

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The Varian 880 AAS is equipped with a sample introduction system pump system (SIPS), GTA100 graphite tube atomizer, and deuterium continuum source background correction. The SIPS system provides multi-point calibration from a single standard and fast on-line dilution of over-range samples. The GTA100 features a Constant Temperature Zone (CTZ) design which provides low detection limits, excellent sensitivities, long tube lifetimes and reduced memory effects. PI: Dr. Jim McGeer PERKIN ELMER MODEL 3100 AAS ATOMIC ABSORPTION SPECTROMETER This AAS is equipped with the lamps required to do all major cations and many heavy metals. The detection limits for most elements is low ppm (i.e. 0.30 ppm), however, heavy metals in natural waters are often difficult to detect because they are usually in the low ppb range. PIs: Chemistry, Co-supervised with Geography and Environmental Studies SIMPLE FIBER OPTIC RAMAN SPECTROMETER (RSI) Raman spectroscopy can be used to study a wide variety of sample types based on a change in polarizability due to vibrational motion following absorption of a photon. PI: Dr. Vladimir Kitaev DNA ANALYSIS BIORAD ICYCLER IQ REAL TIME PCR MACHINE: This real-time PCR instrument enables monitoring while the reaction progresses, allowing for more accurate quantification of the starting template and elimination of postPCR processing. The iCycler accepts 96 well plates and has filters installed for FAM490, Cy3/Vic/Hex/Tet and Rox/texas red. The light source is a tungsten halogen lamp, and the detector is a CCD camera. Protocol development and data analysis are controlled by the iCycler software. PI: Dr. Robin Slawson BIO-RAD MOLECULAR IMAGER GEL-DOC XR The Gel Doc system is equipped with a camera, UV- and white-light illumination, and a filter for ethidium bromide imaging. The detection range covers 3 orders of magnitude. Colony counting and 1-D gel quantification and analysis are conducted using Quantity 1 software. All data must be saved on a memory key and transferred to a separate computer (do not store your data on the computer connected to the Gel Doc). Ethidium bromide very hazardous and a known mutagen: all ethidium bromide stained products must be brought in the lab in a closed container. PI: Dr. Robin Slawson

RESEARCH INSTRUMENTATION HANDBOOK BIORAD PERSONAL FX MOLECULAR IMAGER PHOSPHORIMAGER

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This phosphorimager detects radio-labeled DNA and protein molecules using phosphor screens. The imager accommodates both large (35 x 43 cm) and small (20 x 25 cm) storage phosphor screens. Storage phosphor technology offers the high isotopic sensitivity, with exposure times typically 1/10 that of film. Samples can be labeled using the radioisotopes 32P, 33P, 35S, 14C and 3H. The information obtained from the imager can be used to assess protein metabolism, gene expression, and nucleic acid sequences. Any work involving radioactivity must first be approved by the Environmental/ Occupational Health and Safety Office. PI: Dr. Matt Smith CBS SCIENTIFIC DGGE GEL SYSTEM Denaturing gradient gel electrophoresis can be used to separate PCR products based on sequence differences and can be used to identify single base changes and polymorphisms in DNA. This system includes a pump and gradient maker to prepare gels, allows for buffer heating and agitation, and can run up to 4 gels simultaneously. The generator can power up to 300 V. PI: Dr. Robin Slawson LICOR 4300 DNA SEQUENCER The Licor sequencer can be used for DNA sequencing, microsatellite analysis, and amplified fragment length polymorphism (AFLP) analysis. This instrument is capable of reading up to 1000-1200 bp with very high accuracy using a dual laser system. Up to 64 samples can be loaded in one run. The software can be used to view the electrophoresis results, make manual changes, and to align sequences. PIs: Dr. Scott Ramsay, Dr. Matt Smith

RESEARCH INSTRUMENTATION HANDBOOK MICROSCOPY LEICA DMR HC UPRIGHT BRIGHTFIELD MICROSCOPE This is a research quality microscope with brightfield and differential interference contrast capability. The microscope is equipped with a digital imaging system. PI: Dr. Paul Mallet OLYMPUS BX-51 OPTICAL MICROSCOPE This optical microscope is capable of up to 1500X magnification. It is equipped with dry lenses, which can be coupled to a sensitive UV-Vis spectrometer for microspectroscopy (~10 micron by 10 micron areas). PI: Dr. Vladimir Kitaev OLYMPUS FLUOVIEW FV1000 CONFOCAL LASER SCANNING MICROSCOPE This inverted confocal laser scanning microscope can be used for 3-D imaging, time course experiments, energy transfer visualization, and photobleaching experiments using both live and mounted specimens. The system is equipped with three lasers: a multi argon-ion laser (457, 488, and 515 nm), a helium-neon (green) laser (543 nm), and two diode lasers (643 and 405 nm). The high quality optics include 5 objective lenses: 10x, 20x, 40x, 60x (oil immersion), and 100x (oil immersion). The user friendly software is used for image acquisition and simple analysis. Images can also be viewed on a personal computer using a limited freeware version of the software. This system requires #1 (0.17 mm) coverslips for all objectives except the 40x objective, which has a correction collar. PI: Dr. Diano Marrone HITACHI SU-1510 SCANNING ELECTRON MICROSCOPE

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The SEM is used primarily to gather topographical or morphological information for a variety of specimen types. Some sample composition information can also be collected using the backscattered electron detector. Non-conductive samples can be imaged quickly and easily in the variable pressure mode, or coated with a sputter coater and imaged under high vacuum. The 1510 SEM can obtain a resolution of 3 nm under high vacuum or 4 nm with variable pressure. Samples up to 153 mm in diameter and 60 mm in height can be imaged using this SEM. PI: Dr. Mihai Costea

RESEARCH INSTRUMENTATION HANDBOOK OTHER SPECIALIZED EQUIPMENT ANY-MAZE ANIMAL BEHAVIOUR VIDEO TRACKING SYSTEM ANY-maze is a video tracking system designed to automate testing in a wide range of behavioral apparatus, including various mazes, boxes, tanks, and cages. PI: Dr. Paul Mallet BECKMAN LS 6500 SCINTILATION COUNTER

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The scintillation counter is used for measuring radioactivity in solid or liquid samples. Liquid samples can be prepared in either in the standard 20 ml vial or in a 6 ml miniature vial. The LS6500 can also be used for single photon counting and dual label studies. PI: Dr. Deb MacLatchy, Dr. Matt Smith BECKMAN OPTIMA MAX BENCHTOP ULTRACENTRIFUGE This ultracentrifuge can be used for applications ranging from pelleting to gradient separations for proteins, DNA, RNA, subcellular fractions, membranes, virus, lipoproteins, and other sample types. There are currently two rotors available for this centrifuge: the MLS-50 rotor can accommodate 4 x 5 mL tubes with dimensions of 13 x
51 mm, and can accelerate up to 280,000 x g; the TLA-100.3 rotor can accommodate 6

x 3.5 mL tubes with dimensions of 11 x 32 mm, and can accelerate up to 541,000 x g. PI: Dr. Matt Smith GR-202 ANALYTICAL BALANCE (210 G X 0.0001 G) A precision scale used for mass measurements of up to 210 g. It has a resolution of 0.01 mg and repeatability of 0.02 mg. PI: Dr. Paul Mallet LABCONCO FREEZE DRIER The freeze drier can be used to lyophilize a variety of sample types and volumes for shipping or storage. Compared to other types of freezing, lyophilization causes less damage and allows for the retention of structure and some viability. Samples containing acids or solvents cannot be freeze dried on this instrument. PI: Dr. Masoud Jelokhani-Niaraki LEICA CM3050 CRYOSTAT The Leica CM3050 cryostat is used for cryosectioning delicate samples. The specimen can be oriented very precisely to produce high quality and reproducible thin serial sections. Sample temperature can be lowered to -50 oC. This cryostat can accommodate samples up to 40 x 55 cm in size and sectioning widths can be set from 0.5 to 300 m. PI: CFI-funded shared equipment located in the Animal Care Facility; contact Dr. Paul Mallet before using.

RESEARCH INSTRUMENTATION HANDBOOK METROHM 633 VOLTAMETRIC ANALYZER Voltammetric instrumentation for trace to ultra-trace level metal detection and speciation analysis. The system consists of a dropping mercury electrode (Metrohm 633 VA) and potentiostat unit (Autolab PGSTAT302N). PI: Dr. Scott Smith SARTORIUS SE2 ULTRAMICROBALANCE This ultramicrobalance has a capacity of 2.1 g and 0.1 g readability and is equipped with air buoyancy correction, automatic internal calibration, and draft shield. The data can be automatically transferred to a spreadsheet to speed weighing of multiple samples. PI: Dr. Jim McGeer SHIMADZU TOC-5050 TOTAL ORGANIC CARBON ANALYZER The TOC analyzer can be used to measure total dissolved carbon, inorganic carbon, organic carbon, and non-purgeable organic carbon in water samples. All samples undergo combustion at 680o C, followed by non-dispersive infrared detection. Quantitation is based on a calibration curve, and detection ranges from 50 ppb to 4000 ppm (or 5000 ppm for IC). PI: Dr. Jim McGeer MISONIX S-4000 SONICATOR

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This programmable sonicator features a touchscreen controller and sound enclosure. The output amplitude is controlled from 1-100% giving a greater degree of resolution and the ability to pinpoint the amplitude needed to effectively process the sample. No manual tuning is required. BARNSTEAD MEGA-PURE MP-1 WATER STILL This all-glass still delivers ultrapure Type II water at a rate of 1.4 liters per hour. The vertical condenser design helps ensure high purity water, and the high-temperature cutoff prevents heating element burn-out. PI: Dr. Paul Mallet KAISER REPOKID 5360 COPY STAND This copy stand can be used to photography specimens with minimal shadow on a none-reflective black background. The camera is supported by a column with a cm/inch scale, and the specimen is illuminated by two bright bulbs. The black matt base is 32 by 38 cm in size. PI: Dr. Frederique Guinel

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TRAINING AND USER FEES

USER TRAINING AND CERTIFICATION All users must receive training before initial use. Training includes a brief discussion of theoretical background, hazards, and operating principles, followed by hands-on instrument use. Before training, each user must review the standard operating procedure for an instrument. Please contact the Instrumentation Technician to arrange a mutually agreeable time for training. Previously approved users do not need to be retrained. A list of approved users will be posted near each of the multi-user instruments. A training fee applies for external users only.

INTERNAL USER F EES Internal users are not currently charged for instrument training. User fees have been implemented to alleviate regular maintenance and consumable costs for multi-user instruments. The fees listed in Table 1 will be in effect for the 20102011 fiscal year (May 1, 2010 April 30, 2011). These fees will be adjusted as needed after April 30, 2011 to account for changing consumable costs and increasing maintenance as the instruments age. Fees for new specialized instruments will be appended on an ongoing basis. The use of specialized equipment requires permission from the PI, and user fees for specialized equipment are determined on an individual basis, depending on the experiment and collaboration.

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Table 1: Internal user instrument fees

INSTRUMENT
Steris Prevaccum Sterilizer Miele Glassware Washer Milli-Q Synthesis A-10 Cary Eclipse Fluorescence Spectrometer Cary 50 UV-Vis Spectrometer Beckman LS 6500 Scintillation Counter Beckman Avanti J30I Centrifuge Centrivap Centrifugal Concentrator Aminco/Thermo French Press Olympus FV1000 Laser Scanning Confocal Microscope Hitachi SR1510 Scanning Electron Microscope Acetonitrile DMF Ethyl Ether Glasscontour Dry Solvent system* Hexane Methanol Methylene Chloride THF Toluene Insta-Ice 475 Dry Ice Block Maker* *These costs may be covered directly by the principal users.

USER FEE
$ 0.60 / cycle $ 0.50 / cycle $ 0.40 / liter ----$ 2.15 / sample ------$ 20.00 / hour or $1500 / year $ 35.00 / hour $ 1.53 / 25 ml $ 1.61 / 25 ml $ 1.43 / 25 ml $ 1.35 / 25 ml $ 1.45 / 25 ml $ 1.67 / 25 ml $ 1.77 / 25 ml $ 1.37 / 25 ml $ 4.75 / block

EXTERNAL USER F EES External users must receive permission from a PI before using any of the specialized instruments listed in Table 2. External users must also be trained on any instrument before initial use. Following training, supervision may be required depending on the instrument and whether or not use is part of a collaboration with a science faculty member at Laurier. Any consumables required for instrument operation are also charged to the user. The fees listed in Table 2 will be in effect for the 2010-2011 fiscal year (May 1, 2010 April 30, 2011). Fees for new specialized instruments will be appended on an ongoing basis.

Table 2: Instrument training and user fees for external users*

INSTRUMENT TRAINING FEE (ESTIMATED TRAINING TIME)
$ 35 / hour (2-3 hours)

ASSISTED USE (PER HOUR)

$ 20

UNASSISTED USE (PER HOUR)

$ 10 **

Aviv Circular Dichrosim Spectrometer

Hitachi SU1510 Scanning Electron Microscope Licor DNA Sequencer

$ 70 / hour (1-2 hours) $ 35 / hour (3-4 hours) $ 35 / hour (1 hour)

$ 60

$ 45

$ 20

$ 10 ** $ 10 per hour or $50 per day

Leica CM3050 Cryostat

$ 20

Olympus FV1000 Confocal Laser Scanning Microscope

$ 55 / hour (2-3 hours)

$ 40

$ 30

* These fees apply to academic users only; please contact the Instrumentation Technician for commercial user fees. **An additional fee for consumables may apply

RESEARCH INSTRUMENTATION HANDBOOK TECHNICAL PROBLEMS AND EQUIPMENT DAMAGE

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Technical problems encountered during normal use of a multi-user or specialized instrument must be reported to the Instrumentation Technician immediately, and recorded in the log book. All repairs and the regular maintenance described in the SOP for multiuser instruments will be coordinated or carried out by the Instrumentation Technician. Users should not attempt to repair any of the multi-user instruments. Depending on funding, repairs that are required as a result of normal use will be covered by the Faculty of Science for instruments where the Faculty of Science is listed as the PI. Any damage that is the result of user misconduct or neglect may be charged to the user.