Prepared by: COSUE, BRIAN 2110043

I. Basic Information A. Pathogenic Gram-Positive Bacteria B. Lesson Title: Clostridium tetani C. Year Level: 2nd Year BMLS Students D. Time Allotment: 1 hour II. Behavioral Objectives At the end of the lesson, the students are expected to: A. Be familiar of the morphological and colonial characteristics of Clostridium tetani. B. Know the disease caused and pathogenicity of the bacterium. C. Know the diagnosis, progression, chain of infection and signs and symptoms of tetanus. III. Subject Matter A. Major Concepts 1. The general characteristics of Clostridium tetani. 2. The disease caused by Clostridium tetani. 3. The pathogenicity of Clostridium tetani. B. Content Outline 1. General Characteristics a. Morphological Characteristics i. Gram-positive bacilli ii. Spore-forming iii. Motile iv. Round and terminal w/in swollen sporangium (lollipop shaped) b. Cultural Characteristics i. Round ii. Small with fimbriated margins iii. Beta-hemolytic 2. Disease and Pathogenicity a. Virulence Factor i. Tetanospasmin b. Disease: Tetanus i. Chain of Infection ii. Signs and Symptoms iii. Progression iv. Diagnosis C. Instructional Materials 1. Board & Marker

2. Video presentation D. References/ Resources 1. Reddy P, Bleck TP. Clostridium tetani (Tetanus). In: Mandell GL, Bennett JE, Dolan R, eds. Mandell, Douglas, and Bennetts Principles and Practice of Infectious Diseases . 7th ed. Orlando, FL: Saunders Elsevier; 2009:chap 244 2. http://health.nytimes.com/health/guides/disease/tetanus/overview.html IV. Lesson Proper A. Motivation 1. The students will be shown pictures of the organism microscopically. 2. The students will be shown a video presentation of the signs and symptoms of tetanus. B. Presentation 1. Lecture Discussion 2. Some topics aided with photo/video presentation V. Evaluation Students will be given a ten-point seatwork to be answered on a sheet of paper with the questions: A. Enumerate the characteristics of Clostridium tetani on BAM. 1. Round 2. Beta-hemolytic 3. Small with fimbriated margins B. Identification: 4. The virulence factor of the bacterium. (Tetanospasmin) 5. Another name for lockjaw. (Trismus) 6. The disease caused by the bacteria. (Tetanus) 7. Sustained lock jaw. ( Risus sardonicus) 8. Persistent back spasms with backward arching. (Opisthotonus) 9. Habitat of Clostridium tetani in the environment. (Soil) 10. Infection of newborns through the use of contaminated umbilical clamp. (Tetanus neonatorum) VI. Assignment Students are given a five-point assignment to be written on a piece of paper with the question: A. Why is diagnosis of tetanus primarily clinical instead of making use of diagnostic tests?