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Osmosis Lab Part I

Goal of Lab Document the physical changes that occur to potato cores over a period of two days in different concentrations of sucrose solutions and relate these changes to the phenomenon of osmosis. You will use tables to carefully track your measurement data, graphs to interpret your data, and write a lab report to connect your observations to the biological phenomenon osmosis. Materials and Methods

6 potato cores 6 Beakers Table sugar (sucrose) Cork corer Tape and marker Ruler Accurate scale measuring to at least 1/100 grams Graph paper

You will be assigned to either a salad (red) potato group or a baked (brown) potato group. You will submit your potato cores to a total of six treatments. Your experimental treatments are: 0%, 5%, 10%, 15%, and 20% sucrose solution. You will also conduct an experiment to determine the concentration of a mystery solution provided by your instructor. Write down which potato you were assigned here _________________.

1. Prepare your sucrose solutions by mixing sugar and water in a beaker. The % sucrose you want for
your solutions is equal to the # grams of sucrose you should use. For example if you want 5% sucrose solution then measure out 5 grams of sucrose. Add the 5 grams of sucrose to water. The amount of water you need is [100 the grams of sucrose]. So for our example you take 100 5 = 95 ml of water. Label each beaker as you go using tape and markers. This is really important!!! Fill in the table below you mix up your solutions correctly.

% sucrose 0 5 10 15 20

Grams sugar

ml water

2. Now you need to get your potato cores ready. Use one of the cork borers to get two cores from your
assigned potato. Cut the cores down to approximately 30 mm using a ruler. Cut the core as close to

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vertical as you candont angle the cuts. Also make you should only use pieces of potato core that are shaped like a cylinder. Some cores will be missing parts. Fill in the table below to track the data you need for your cores. Use the appropriate number of significant figures in your dataask what this means if you do not know!

Name/Hour__________________ Group #_________

Core # Start 1 2 3 4 5 6

Length Day 1

Day 2

Start

Width Day 1

Day 2

Start

Weight Day 1

Day 2

3. Each beaker should be labeled with its % sucrose concentration, the core you added, one of your
group members names, and your class hour. Set the beakers aside where they will not be disturbed. Percent Change We will be using percent change to document any changes in our potato cores. Percent change is defined by the following equation: % change = {[final measurement initial measurement] [initial measurement]} X 100. You can use this for all of the data we are tracking above. Please set up a table in the space below to track % change for each of your potato cores.

Questions 1. Which of the solutions you prepared is most likely to be hypertonic compared to your potato cores? Justify your answer.

2. What do you think will happen to your potato cores if they are placed in a hypertonic solution
(reference the data you are collecting)?

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3. Which of the solutions you prepared is most likely to be hypotonic compared to your potato cores? Justify your answer.

4. 5.

What do you think will happen to your potato cores if they are placed in a hypotonic solution? What do you think will happen to your potato cores if they are placed in an isotonic solution? Justify your answer.

6.

Based on your answers for 1-5, create a graph that shows what you think will happen to the % change in the weight of your potato cores in the solutions we are using. Focus on the general pattern of what the graph should look like and dont worry about the actual numbers. Please label the axes appropriately.

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7. How could you use your graph above to predict the concentration of a mystery solution if you knew how much the mass of the potato core changed?

8.

This lab states we are studying osmosis. How does osmosis relate to diffusion? How is osmosis similar to diffusion? How is it different? You may use a Venn diagram below or a t-chart to organize your ideas.