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Abstract
The molecular interactions and cell-biological mechanisms behind the adhesive behavior of a cell are not properly understood.
In the last decades, the development of bio-protheses and the hope for elaboration of successful cell replacement therapies
accelerated the search for appropriate scaffold materials and conditions which may help to keep cells alive and functioning on
artificial surfaces.
For understanding some crucial steps of cell-attachment, the initial phases of attachment of two different types of cloned cells –
NE-4C neural stem and MDCK epithelial cells - were analyzed by optical waveguide light mode spectroscopic (OWLS) methods,
34 PL
Evanescent light
0
19
0 PLL+F
14
OWLS methods allow quantifying the deposition of material in a 0
thin (<150 nm) layer above the solid sensor surface. In terms of 9
cells, it can provide data on focal contacts and adhesion sites, 4
while the rest of the cellular mass remains out of the field of 0
-
detection. 23 23 24 24 25 25 26 26 27
2 7 2 7 Time
2 7 2 7 2
[min]
Surface chemistry, sensitization of the
The length of the latency period depends on the adhesivity of the
sensor surface provided substrate
Bare or amino-functionalized sensor surfaces were coated with
different attachment-molecules including poly-L-lysine, laminin,
fibronectin, and RGD peptide-mimetics.
Deposition of NE 4C cells on laminin
34
Cell preparations 0
Cell suspensions in protein-free, artificial cerebro-spinal fluid were 29 Active processes
37oC
Absorbed mass [arb.units / area]
D e p o s itie d m a te ri a l
4
200 200 0
[a r b .u n its ]
[a r b .u n its ]
-
0 10 20 30 40 50 60 70
150 150 10
Time [min]
100 100
Conclusion
50 50
The ∆N versus time function clearly distinguished the active
0
attachment from passive sedimentation.
0 The results indicate that OWLS techniques allow rapid evaluation
5 15 25 35 45 55 65
5 15 25 35 45 55 of cell – matrix interactions and provide tools to characterize the
Time [min] Time [min]
composition of sets of adhesion molecules on cell surfaces.
MicroVacuum Ltd. H-1147, Kerékgyártó u. 10., Budapest, Hungary Phone: + 36 1 252 1991; +36 1 467 0108 Fax: +36 1 221 7996
Web: http://www.owls-sensors.com/; E-mail: info@owls-sensors.com Copyright @ Microvacuum, 2006