Group 1KC 31003-Bioprocess principles 3rd year Bachelor of Engineering with honors (Chemical engineering) Brain teasers (To

be solved by students on 15 May 2012 in the Class) Problems: (1).Determine initial rate of product (P) formation from enzyme X and the substrate Y that form the complex XY. The reaction kinetics is given by X+Y XY X+P

(2).Determine the initial rate of product (P) formation from enzyme X and substrate Y that form the complex XY. The reaction kinetics is given by X+Y (XY) 1 (XY)2 X+P

(3).Determine the effect of diffusion (in percent) on the reaction rate constant for a first order reaction for an immobilized enzyme of radius r=1nm, rate constant k=310-8 mol-1 s-1 Deff = 6.510-7 cm2/sec.

Group 2-KC 31003-Bioprocess principles 3rd year Bachelor of Engineering with honors (Chemical engineering) Brain teasers (To be solved by students on 15 May 2012 in the Class) Problems: (1).The growth rate of E. coli be expressed by Monod kinetics with the parameters of max = 0.935 hr-1 and KS = 0.71 g/L (Monod, 1949). Assume that the cell yield YX/S is 0.6 g dry cells

per g substrate. If CX0 is 1 g/L and CS1= 10 g/L when the cells start to grow exponentially, at t0 = 0, show how ln CX, CX, CS, dlnCX /dt, and dCX /dt change with respect to time
(2).Immobilized cell or enzyme technology for formation of biocatalyst has been a valuable tool in many bioprocesses. Among the technologies, entrapment of biological materials within calcium alginate gel beads is the most commonly used. a) Explain why calcium alginate has been a popular as immobilization material. Suggest alternative materials suitable for immobilization b) Describe at least two purposes for using immobilization system in such fashion and give example on how the each purpose is served. c) Describe at least three methods for large-scale production of calcium alginate beads and state the pros and cons of each method. d) Describe one industrial application for each immobilized cell and enzyme system with the aid of PID diagram. e) Describe the problems that envisaged when using immobilized cell/enzyme technology. (3).A by-product of fermentation process has shown to be inhibitory to cell growth. From the data
below: a) Obtain the kinetic parameters m, ks. b) Determine the type of inhibition by the inhibitor. Justify your answer and what do you think of the data accuracy. c) Determine inhibition constant, kp. What can u do to improve the reliability of the value?

Specific growth rate of cells at different glucose concentration in fermentation medium containing different lactic acid concentration.

By (g/L) 0.00

Glucose (g/L) 3.00 4.00 5.00 8.00 12.00 16.00 20.00

Specific growth rate (hr-1) 0.15 0.17 0.19 0.25 0.26 0.29 0.41 0.08 0.10 0.11 0.11 0.17 0.23 0.07 0.07 0.09 0.10 0.15 0.18

20.00

3.00 4.00 5.00 6.00 10.00 20.00

25.00

3.00 4.00 5.00 8.00 12.00 20.00