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Thesis/project report submitted to Graphic Era University, Dehradun, In partial fulfillment of the requirement for the degree of Bachelor of Technology In Biotechnology by Mr. Bipin Badoni

Under the supervision of Dr. XXXXXXXXX

Official address of external supervisor ( if any)

Dr. XXXXXXXXXX
Department of Biotechnology Graphic Era University Dehradun, UK., India

CERTIFICATE This is to certify that the thesis, entitled Effect of --------------- --------------mutants submitted to Graphic Era University, in partial fulfillment of the requirements for the Degree of M. Sc. Biotechnology/Microbiology or B. Tech. Biotechnology, is a record of original research work done by Mr./Ms.------- during the period of dd/mm/yy to DD/MM/YY of his study in the department of--------, at -------institute/university/company under my supervision and guidance and the thesis/project report has not formed the basis of the award of any Degree/Diploma/Associateship/Fellowship or other similar title to any candidate of any university.

Counter signed Head of Institution/department Supervisor/Mentor

CERTIFICATE This is to certify that the thesis, entitled Effect of --------------- --------------mutants submitted to Graphic Era University, in partial fulfillment of the requirements for the Degree of M. Sc. Biotechnology/Microbiology or B. Tech. Biotechnology, is a record of original research work done by Mr./Ms.------- during the period of dd/mm/yy to DD/MM/YY of his study in the department of--------, at -------institute/university/company under my supervision and guidance and the thesis/project report has not formed the basis of the award of any Degree/Diploma/Associateship/Fellowship or other similar title to any candidate of any university.

Counter signed Head of Institution/department Supervisor/Mentor

DECLARATION

I, (name of candidate) hereby declare that the thesis/report entitled Effect of ---------------------------- mutants submitted to Graphic Era University, in partial fulfillment of the requirements for the Degree of M. Sc. Biotechnology/Microbiology or B. Tech. Biotechnology, is a record of original and independent research work done by me during dd/mm/yy to DD/MM/YY under the supervision and guidance of name of mentor/supervisor with designation and official address and the thesis has not formed the basis of the award of any Degree/Diploma/Associateship/Fellowship or other similar title to any candidate of any university/institution.

ACKNOWLEDGEMENT

First of all, I bow in reverence of Almighty, God the creator of this universe for providing enough patience, zeal and strength that enable me to complete this project successfully. The inspiration, enthusiasm and passion of attaining higher education is the outcome of the massive contribution of my mentors (My Mom, Dad ) who always remained inclined to enlighten my glimmering future and beckoned the hope of mending an extremely scintillating career. And this project is the complete reflection of their efforts.

I wish to express my deep sense of gratitude and humble thanks to Dr., Director,

I had the privilege to work under Mr. Paratpar Sarkar, Asst. prof Biochemistry, Department of Biotechnology, Graphic Era University. His overly enthusiasm and integral view on research and his mission for providing only high quality work and not less, has made a deep impression on me. His invaluable guidance, vital and thought provoking discussions and professional approach were largely responsible for the finalization of the project.

I am heartly thankful to Dr. Nishant Rai, HOD, Department of Biotechnology, Dr. Naveen Bajpai, project coordinator Thakuri and all the technical staff of the Nuclear Medicine Division, INMAS who were of great help while pursuing my project.

I express my sincere thanks to Mr. for their constant help and valuable experienced guidance, encouragement at every step of my project work.

I am thankful to my lab mates Mr., who helped me a lot when I needed.

My parents are the pillar of strength for me, who have supported, loved and cared for me, I thank to them from my inner soul. I am grateful to God who has gifted me such a wonderful parents. Whatever I have achieved in my life is because of the sacrifices and efforts of my parents and love of my brothers and sisters.

BIPIN BADONI

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Project report submitted to Graphic Era University, Dehradun, by Mr. Bipin Badoni For Industrial Training (TBT608) Of the course B. Tech. Biotechnology, VIIIth semester.

Under the supervision of Dr. XXXXXXXXX Asst. Prof. Mr. Paratpar Sarkar Official address of Department of Biotechnology Graphic Era University Dehradun, UK., India

INTRODUCTION
Over 50% of the milk produced in India is Buffalo milk, and 45% is Cow milk, Indian raw milk composition is as follows: Type of milk Protein % Fat % Milk sugar % Ash % Water % Buffalo/Yak 3.60 7.40 5.50 0.80 82.70 Cow 3.50 3.70 4.90 0.70 87.00 Where as pasteurized packaged milk as specified can be divided according to fat content as follows: Type of milk Fat % (Solids-non-fat) SNF % Whole/Full cream milk 6.00 3.60 Toned/standard milk 3.00 3.50 Double toned/skimmed milk 1.50 3.50 Presently only 12% of the milk market is represented by packaged and branded pasteurized milk, valued at about Rs.8,000 crores. plastic pouches replaced the bottles. Plastic pouches made transportation and storage very convenient, besides reducing costs Milk packed in plastic pouches/bottles has a shelf life of just 2-3 days. Milk production in India is pegged at about 122 million tonnes per annum. Of this, processed milk accounts for 35 per cent, while unprocessed (which is the unorganised sector) is 65 per cent. 1.1 The Indian dairy industry is contributing significantly to the country's economy, besides improving the health standard by increasing the nutrition value of the food. The value of output from Dairy Sector increased to Rs.5,00,510 million in 1994-95 from Rs.2,75,080 million in 1990 and is expected to reach the level of Rs. 8,50,000 million by the year 2000 A.D. 1.2 India occupies first position in the world having a total bovine population of 288 million compared to the world's total bovine population of 1420 million. Need of the study

The project was an attempt to know the reasons behind low shelf life of dairy milk. Despite of being the best in the industry,and having the first mover advantage and the brand name, the like Dairy India ,Reliance , Paras, Madhusudan and others, consumers find difficult to store the milk for more than 2 days.. With the help of the responses given by the consumers and data analysis, the company will be able to understand its strengths, weakness , opportunities and threats. The survey report submitted by me will assist the company to take right decisions to increase the turnover and market share. Objective of the study The objectives of the study are the following :1. The main objective of this project is to probe the reasons for decreasing market share of Mother Dairy and to find out the measures that can be adopted to increase the sales 2. To study the distribution channel in the retail chain of Mother Dairy 3. To identify the issues related to Mother Dairy retailers and distributors 4. To get the retailers feedback from specific regions 5. To add up new retailers in the area Summer Internship Report, PGDM 09-11 | 6

6. To study Mother Dairy positioning in the mind of the consumers vis--vis competitors 7. To find out the factors that affect consumers decision to purchase milk 8. To find out short term problem in every sub-region that can be sorted out 9. Suggesting viable recommendations to be implemented in the area Mother Dairy markets approximatley 3.2 million litres of milk daily in the markets of Delhi, Mumbai, Saurashtra and Hyderabad. Mother Dairy milk has a market share of 66% in the branded sector in Delhi where it sells 2.5 million litres of milk daily and undertakes its marketing operations through around 1400 retail outlets and over 1000 exclusive outlets of Mother Dairy.

Reliance: Reliance Dairy Foods, a subsidiary of Reliance Retail, is muscling into the country's branded milk product market with a new brand that will take on established players such as Amul, Mother Dairy, Nestle The organised sector process about 1.3 crore tonnes annually while the unorganised sector processes about 2.2 crore tonnes. India's total milk production totals 10.28 crore tonnes. Paras: . These facilities enable us to cater to the needs of our clients by selling over 2, 50,000 liters of milk per day in Delhi Metro. PARASs history reflects back to 1960, when the procurement of milk started with 60 Liters of milk. The only fundamental that worked right from day one is the quality, which got reinforced everyday of progress. Ch. VedRam, the founder and promoter of our company is one of the connoisseurs in the dairy farm industry. Ved Ram & Sons started as our partnership company in April 1986. Our first unit was established in 1987 under company's name VRS Foods Limited and since then In tune with the rapidly changing technology, production units are well armed with the latest equipment. Dairy india: Company Overview Gopaljee RSD Group was started with a thought of providing a wholesome nutrition to the people through their available range of products including dairy range as well. They started their operations with biscuits and other FMCG's and then diversified into Dairy industry in the year 1989. During the same year they established their first factory plant at Siyana (Distt. Bulandshahar) having a handling capacity of 75,000 - 1, 25,000 liters per day. During the last 20 years, Gopaljee RSD Group has earned a reputation of a nutrition provider all over India.