SEX DETERMINATION

- Presence and abcence of extra sex chromosome direct effect on tooth size, shape, contour. - Sex determination useful forensic add in determination od crime and identification

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BY DNA TYPING

SOURCE OF DNA
chamber and redicular cannal

DNA From coronal pulp

endodontic access

a.
b.

conventional Crusing entire tooth

c.
d.

Vertical split Horizontal method

20 TO 70 Pico gram DNA required

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AMEL GENE
IN MALE IN FEMALE

X Y
AME L am el AME L

X X
AME L

The human female have tow identical AMEL gene and male have diferntial one on x chromosome and one on y these genes can be identify them this is called as DNA TYPING. PCR can be used when small amount of DNA avelible 8/14/12

Sex determination using pulp

It can be done by fluorescence

Sex determination by pelatel dimension

The race and sex can be identified by craniofacial remains.

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By skull
Male skull can be identyfied by prominent temporal line, mastoid process, nuchal line, Super cilliary arches and ridges. They are smother in 8/14/12

Click to edit Master subtitle style

RACE

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IDENTIFICA TION

RACES
Caucaso id
Shovel shaped incisor Lower 1stmolar longer and once tapering.

Negr oid
Mongolo id
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Small and square lower 1stmolar Shovel shaped incisor Rounded and large lower 1st molar.

Eski mos Amer icon india ns RACE

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Race identification by size of teeth

Smaller teeth seen in yementies ,flipinos and indian jet. Larger teeth is seen in melanasians(Nasioe) , Australian ,Aborigines ,South American indian tribes. In termediate sized teeth are seen in Taiwanese Aborigines And Australian whites respectively
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Mongoloids have larger lateral incisor

AGE ESTIMATION

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VISUAL AND CLINICAL METHODS METHOD

A.ERUPTION OF TEETH

Time of eruption

stage of eruption

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B.SPACE BETWEEN DECIDOUS TEETHS

Gastafson said that before 4 yr of age an spacing is present between decidous teeth than can be used in identify either patent is aged than 4 year or not.

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C.TEETH NUMBER
It can be used in emergencies
Eruption of deciduous teeth CI 6-8 m 7-9 m LI U 7-9 m L 10-12 m 1stm - 12-14 m Canine 17-18 m 2ndm 20-30 m

Permanent teet

1stm -

CI yrs.

LI - yrs.

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1stbicusped

RADIOGRAPHIC AND HISTOLOGICAL METHOD

RADIOGRAPHIC METHOD
HISTOLOGI CAL METHOD

By eruption of teeth radigraph by development of 2nd molar root structure and 3rd molar development by masselar 8/14/12

BY determining stage of development of tooth as bell ,cap.