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Medical Microbiology
Dr.T.V.Rao MD
Dr.T.V.Rao MD
What is Microbiology?
Microbes, or microorganisms are minute living things that are usually unable to be viewed with the naked eye. What are some examples of microbes?
Bacteria, fungi, protozoa, algae, viruses are examples! Some are pathogenic Germ refers to a rapidly growing cell.
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What is Microbiology
Microbiology study of microorganisms (simple forms of life visible only with a microscope) Microorganisms may be
Normal flora Pathogenic
Why is it Important?
Infection is one of the most important causes of mortality and morbidity in the population. Approximately 30% of hospital patients are on antibiotics at any one time 1 in 10 patients acquires an infection whilst in hospital.
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1673-1723, Antoni van Leeuwenhoek (Dutch) described live microorganisms that he observed in teeth scrapings, rain water, and peppercorn infusions.
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Pioneers of Microbiology
Antoni van Leeuwenhoek, DE (1673)
First observed live microorganisms (animalcules)
Pioneers of Microbiology
Louis Pasteur (1822-1895), Chemist
Fermentation (1857) Pasteurization: heat liquid enough to kill spoilage bacteria (1864) Vaccine development rabies Proposed the germ theory of disease Proposed aseptic techniques (prevent contamination by unwanted microbes) Director of Pasteur Institute, Paris (1894)
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Pioneers of Microbiology
Joseph Lister, UK (1867)
Used phenol (carbolic acid) to disinfect wounds First aseptic technique in surgery
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Microbiology
The study of organisms too small to be seen without magnification
bacteria viruses fungi protozoa Helminths (Parasites) algae
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Classification of Organisms
All living organisms are classified into:
Kingdom Phyllum (family) Genus Species
Organisms that can cause disease are many and varied and include:
Viruses Bacteria Fungi Parasites
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Impact of pathogens
Nearly 2,000 different microbes cause diseases 10 B infections/year worldwide 13 M deaths from infections/year worldwide
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Characteristics of microbes
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Scientific Method
Form a hypothesis - a tentative explanation that can be supported or refuted by observation & experimentation A lengthy process of experimentation, analysis & testing either supports or refutes the hypothesis. Results must be published & repeated by other investigators.
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Spontaneous generation
Early belief that some forms of life could arise from vital forces present in nonliving or decomposing matter. (flies from manure, etc)
(1822-1895)
Robert Koch
Established a sequence of experimental steps to show that a specific m.o. causes a particular disease. Developed pure culture methods. Identified cause of anthrax, TB, & cholera.
(1843-1910)
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Kochs work on diseases and diagnostics culminated with the creation of what are now known as Kochs Postulates. Kochs Postulates are the 4 steps necessary to confirm if a suspected pathogen is indeed the cause of a disease.:
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How do we know that a given pathogen causes a specific disease? Koch's postulates
the pathogen must be present in every case of the disease the pathogen must be isolated from the diseased host & grown in pure culture the specific disease must be reproduced when a pure culture of the pathogen is inoculated into a healthy susceptible host the pathogen must be recoverable from the experimentally infected host
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Koch's Postulates
1. Microorganisms are isolated from dead animals
2.
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Figure 14.3, steps 12
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Figure 14.3, steps 34
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Joseph Lister
Joseph Lister, was a British surgeon and a pioneer of antiseptic surgery. By applying Louis Pasteur's advances in microbiology, he promoted the idea of sterile surgery while working at the Glasgow Royal Infirmary. .
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Joseph Lister
He instructed surgeons under his responsibility to wear clean gloves and wash their hands before and after operations with 5% carbolic acid solutions. Instruments were also washed in the same solution and assistants sprayed the solution in the operating theatre. One of his additional suggestions was to stop using porous natural materials in manufacturing the handles of medical instruments.
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Joseph Lister
Lister successfully introduced carbolic acid (now known as phenol) to sterilise surgical instruments and to clean wounds, which led to a reduction in postoperative infections and made surgery safer for patients. Dr.T.V.Rao MD
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Domains
Eubacteria -true bacteria, peptidoglycan Archaea odd bacteria that live in extreme environments, high salt, heat, etc Eukarya- have a nucleus, & organelles
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Naming Microorganisms
Binomial (scientific) nomenclature Gives each microbe 2 names
Genus - noun, always capitalized species - adjective, lowercase
Bacteria
500-800nm Capable of independent replication Cause of most infections seen in hospital Pneumonia, bacterial meningitis, cellulitis, UTI Many different species Treated with antibiotics
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Viruses
Smallest known infectious agents Subcellular microorganism
Have only nucleic acid surrounded by a protein coat Must live and grow in living cells of other organisms
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Hepatitis virus
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Viruses
Small (50-300nm)
Unable to replicate independently
Viruses (cont.)
Illnesses caused by viruses
Colds Influenza Croup Hepatitis Warts
AIDS Mumps Rubella Measles Herpes
Fungi
Complex, large organisms Eukaryotes (as are humans!) Divided into yeasts & moulds Cause a range of diseases e.g.:
Thrush Athletes foot Invasive & allergic Aspergillosis
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Fungi
Eukaryotic organisms with rigid cell wall Yeasts
Single-celled Reproduce by budding
Superficial infections
Athletes foot Ringworm Thrush
Molds
Large, fuzzy, multicelled organisms Produce spores
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Multicellular Parasites
Organisms that live on or in another organism and use it for nourishment
Parasitic worms
Usually due to poor sanitation Roundworms Flatworms Tapeworms
Parasitic insects
Bite or burrow under the skin Mosquitoes Ticks Lice mites
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Protozoans
Single-celled eukaryotic organisms, larger than bacteria
Found in soil and water Illnesses
Malaria Amebic dysentery Trichomoniasis vaginitis
Protozoan Trichomonas vaginalis
Normal Flora
Human beings are not microbiologically sterile. We are ALL covered with bacteria, fungi and some parasites. Skin, nose, mouth, gastrointestinal tract ~109 bacteria per gram of faeces Each person carries more non-human cells on their body than their own
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Generalized symptoms
Fever Tiredness Aches Weakness
Normal flora
Provides a barrier Can cause an infection
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2. Obtain specimen(s)
Label properly Include presumptive diagnosis
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4. Culture specimen
Culture medium contains nutrients Examine culture visually and microscopically
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Programme created by Dr.T.V.Rao MD for Medical and Paramedical Students in the Developing World Email doctortvrao@gmail.com
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