Wound Healing, Tissue Engineering and New Materials

Roger W. Yurt, M.D. William Randolph Hearst Burn Center

Burn Center
Clinical care of burn injured patients including smoke inhalation and carbon monoxide poisoning Clinical care of patients with acute and chronic wounds eg, purpura fulminans Clinical care of patients with exfoliating skin disease - TENS

Skin bank

Hyperbaric Oxygen Program

(Partial thickness)

Superficial

Deep

(Full thickness)

Wound Closure
Life-saving - large surface areas, closure ASAP Cosmetic - life time deficits/defects Function joint, tactile, sight, oral

Life-saving
Excise &Autograft Excise & Allograft Recrop Autograft Excise & Allograft Recrop Autograft

Life-saving
Excise &Autograft Excise & Integra Recrop Autograft Excise & Allograft Recrop Autograft

Integra Dermal Regeneration Template

Silastic membrane
Collagen, glycosaminoglycan matrix

Collaboration with School of Chemical and Biomolecular Engineering

Abraham Stroock Microfluidic wound dressings Cabodi M et al J. Biomedical Materials Research

Concept Active Dressing

Dressing
vapor barrier

Inputs
hydration sanitation drug delivery gene delivery

mechanical barrier

Outputs
diagnostics patient profiling biochemical analysis

flow
wound bed

Interface
biochemical regulation
mechanical stimulation perfusion of tissue

Technical Approach: Soft Lithography

silicone monomer

micro-molding

micro-mold polymerization

release

release

sealing fluidic connections

photo-patterning
UV light

Model System

Function Extraction

Extraction

Function Delivery

Delivery

Prototype Active Wound Dressing

Collaboration with College of Human Ecology

C.C. Chu Department of Fiber Science and Apparel Design

Newly Invented Biodegradable Biomaterials: Poly(ester-amide) (PEA)

An example of PEA: amino acids as main building blocks
O O O O O O

C (CH2)4 C NH CH C O (CH2)6 O C CH NH C (CH2)4 C NH (CH2)4 CH NH CH2CH(CH3)2 CH2CH(CH3)2 0.75 COOH

Adipic acid L-Leucine 1,6-Hexanediol L-Leucine Adipic acid L-Lysine

0.25 n

An elastomer: biodegradable rubber band

Load (g)
GJ-X

Strain (%) 881.879.9

Stress (Kg/mm2) 0.10480.0041

Modulus (Kg/mm2) 1.5900.449

Toughness (Kg-mm) 1.02100.1354

133.1 5.2

C. C. Chu, et al., US Patent 6,503,538, January 7, 2003

Electrospun PEA Fibrous Mat

1 inch Video insert

SEM Image of 3D Microporous PEA Fibrous Structure

PEA control (no GN)

PEA w/ GN loaded

Fiber diameter: 1/100 3/100 of human hair

Collaboration with the Department of Biomedical Engineering

Moonsoo Jin, C.C. Chu A novel growth factor and matrix mimeticenhanced biodegradable scaffold for skin tissue engineering

Collaboration with the Department of Biomedical Engineering

Recently funded seed grant Jin growth factors, matrix-mimetic production, conjugation and cell culture Chu 3 dimensional PEA scaffold design and fabrication Burn Center animal laboratory and clinical application

Burn Research Team

Palmer Q. Joe Bessey, M.D. Joe Turkowski, M.D. Suzanne Schwartz, M.D. Research co-ordinators: Nicole Alden, RN, MPH Angela Rabbitts, RN, MS