Escolar Documentos
Profissional Documentos
Cultura Documentos
Cell Size
growth
mitosis
3 Time
Cell 1
Cell 2
Multicellular Organism 1
Lower SA to V ratio
leads to
Cell Size
Time
Fig 19-32
Figure 4-2
11
12
Figure 11-4
b) Cell 2: 5m x 5m x 5m
c) Organism 3: 125(1mxm1xm1) d) Cell 4: 0.125m x 5m x 200m
5
200
15
0.125
Strategies to Increase Surface Area Long, thin cells greater surface area:volume
Muscle fiber
Neuron
16
What about egg cells? Do egg cells break the SA:V rule?
Figure 1. Comparisonof egg sizes. Ostrich egg (right), compared to chicken egg (lower left) and quail eggs (upper left).
Photo by Rainer Zenz.
Metabolically inactive Mostly lipids (fats), storage materials Actual viable cell component is very small
May involve:
Cytoskeleton
- Motor proteins + filamentous tracks
Carrier Proteins
- Transport across membranes
http://bio1151.nicerweb.com/med/Vid/Campbell7e/CytoplasmicStream-V.swf
Question to ponder: What factors make it possible for eukaryotic cells to be so much larger than prokaryotic cells?
Ave. Size of Prokaryotic Cell ~1-5 um diameter Ave. Size of Eukaryotic Cell ~10 100 um diameter
22
Eukaryotic Cell
e.g. typical Animal cell
ENDOPLASMIC RETICULUM (ER)
Smooth ER
Plasma membrane
CYTOSKELETON
Microfilaments Intermediatefilaments Microtubules
Ribosomes
Microvilli
Golgi apparatus Peroxisome Mitochondrion Lysosome Similar to Fig 4-5
26
Eukaryotic Cell
e.g. typical Plant cell
Nuclear envelope NUCLEUS
Nucleolus Chromatin
Centrosome
Central vacuole
Golgi apparatus
Mitochondrion Peroxisome Plasma membrane Cell wall Plasmodesmata Wall of adjacentcell Chloroplast
In plant cells but notanimal cells: Chloroplasts Central vacuole and tonoplast Cell wall Plasmodesmata
27
Cell Fractionation
Separation of a cell structures/components Two phases 1. Homogenization
lysing cells open chemicals, enzymes, or sound waves
2. Centrifugation
Homogenize tissue
Mouse liver
Centrifugation
Use of centrifugal force to separate mixture(s)
Fixed-angle Swinging-bucket
Similar to Figure12A-2
Larger/denser objects sediment more quickly (large S value) Smaller/ less dense objects sediment less quickly (smaller S value)
Figure 12A-3
Cytoplasm
- Internal contents of cell - Contains: Cytosol
~semi-fluid material Organelles (eukaryotes)
Subcellular structures
e.g. ribosomes
Plasma Membrane
4-8nm (40-80 ) thick layer of lipids + protein Boundary between cell and external environment
- defines cell - regulates movement in/out - mediates communication with external environment (e.g. receptors)
Fluid Mosaic
- many components (mosaic) - flexible, dynamic structure; not static
38
39
40
Functions
mechanical strength growth/development protection
Fig 17-24
41
Archaea
4 known variations: a. Sulfated polysaccharides b. Glycoproteins stabilized by Na+ c. S-layers protein chain mail d. Pseudomurein (shown below)
NAG NAT polysaccharide Cross-linked with peptides
Gram stain
Tool to ID bacteria based on cell wall characteristics
Cells stained with violet dye Rinse with alcohol Stain again with counter-stain (usually red dye)
Lipopolysaccharide Peptidoglycan layer Plasma membrane Protein Grampositive bacteria 20 m Gramnegative bacteria
Cell wall
(a)
Gram-positive: -Simple cell walls - cell walls have large amount of peptidoglycan - traps violet dye in the cytoplasm - alcohol rinse does not remove the violet dye, which masks the added red dye. - Cells appear violet after counter-stain is added
(b)
Gram-negative: -Complex cell walls with less peptidoglycan - Cell wall located in layer between -PM and an outer membrane - outer membrane also has lipopolysaccharides -violet dye is easily rinsed from the cytoplasm, - cells appears pink/red after counter-stain is added
Examples of Pathogenic Gram Yersinia pestis (Plague) Bordetella pertussis (Whooping cough) Chlamydia tachomatis (STD)
Gram Stain
Used as a quick diagnostic tool Examples:
Classification of new species
Based on CW characteristics Also preserves shape (e.g. spiral, rods)
Abstract We performed a prospective study in patients with tunneled catheters to assess the validity of Gram stain and superficial culture for anticipating catheter exit-site infection and hemodialysis catheterrelated bloodstream infection. The sensitivity and negative predictive value were high, and we succeeded in identifying a subpopulation at low risk of infection. Diagn Microbiol Infect Dis. 2013 Dec 17. pii: S0732-8893(13)00650-0. doi: 10.1016/j.diagmicrobio.2013.12.008. [Epub ahead of print] Copyright 2013 Elsevier Inc. All rights reserved.
- Penicillin
Interferes with peptide synthesis and peptidoglycan cross-linking
Penicillium fungus (DIC image)
Fosfomycin
Interferes with peptidoglycan biosynthesis
Brightfieldimage of Streptomyces fradiae
Nucleus
Stores DNA = CONTROL CENTER Large organelle
~5-6um diameter ~10% total cell volume
Nuclear Pores
Regulated openings through Nuclear Envelope
Openings controlled by Nuclear Pore Complex (NPC) NPC controls movement of substances in/out of Nucleus
Q: What sorts of molecules are moving in/out of nucleus?
Nuclear Lamina
Network of intermediate filaments (components of the cytoskeleton)
Nucleolus
Region within the Nucleus Clustered regions of ribosomal RNA genes surrounded by specific RNAs & proteins Site of ribosomal subunit synthesis Q: How do ribosomal subunits exit the nucleus? Q: What happens after they leave the nucleus?
Nucleus
(SEM)
54
Ribosome
Found in all cell types (pro/eukaryote),and certain organelles
Not an organelle
25 30 nm
55
The process by which genetic information coded in messenger RNA directs the formation of a polypeptide sequence is called____________.
a) b) c) d) e) Transcription Transformation Translation Transgression None of the above
Eukaryote