SUVIDHA TYAGI BSC III (BIOTECH) ROLL NO 111061

TISSUE

CULTURE INVOLVES THE CULTURING OF WHOLE ORGANS OR TISSUE FRAGMENTS OR THE DISPERSED CELLS ON A SUITABLE NUTRIENT MEDIUM. OF TISSUE CULTURE

TYPES 1.

CELL CULTURE. 2. ORGAN CULTURE.

 THECALL

LINE IMPLIES THE PRESENCE OF PRESENCE OF SEVERAL SIMILAR OR DIATINCT CELL LINEAGES. A CELL LINE IS PRODUCED BY SUBCULTURING OF PRIMARY CELL CULTURE AND IS MORE HOMOGENEUS. AMONG THESE CELL LINEAGES, IF A PARTICULAR CELL LINEAGE HAS MAINLY SPECIFIC TYPE OF CELLS, TI IS CALLED A CELL STRAIN. A. FINITE CELL LINES B. CONTINUOUS CELL LINES

cell lines is produce by sub culturing of primary cell culture is more homogeneous among theses cell lineages if a particular cell lineage has mainly specific type of cell its is called a cell strain.

Various cell lines

WHAT IS SERUM
The serum is the component that is neither a blood cell (serum does not contain white or red blood cells) nor a clotting factor; it is the blood plasma with the fibrinogens removed. Serum includes all proteins not used in blood clotting (coagulation) and all the electrolytes, antibodies, hormones, and any exogenous substances (e.g., drugs and microorganism).

SERUM (PROTEIN-FREE PLASMA OBTAINED AS EXUDED FLUID FROM COAGULATED BLOOD) IS ONE OF THE VERY IMPORTANT COMPONENTS OF ANIMAL CALL CULTURE MEDIA. IT MAY BE USED AS SUCH AS NATURAL CULTURE MEDIUM OR IS ADDED (ABOUT 5-20%) TO ARTIFICIAL MEDIA. ADVANTAGES (I) IT PROVIDES CERTAIN GROWTH FACTORS WHICH PROMOTE CELL PROLIFERATION. (II) IT PROVIDES A NUMBER OF PROTEINS FIBRONECTIN, WHICH PROMOTE CELL ADHESION TO THE SUBSTRATE. (III) IT IS ALSO THE SOURCE OF MANY ORGANIC NUTRIENTS LIKE AMINO ACIDS, LIPIDS, VITAMIN ,POTASSIUM, (IV) IT ALSO ACTS AS BUFFER AND MAINTAINS THE PH OF CULTURE MEDIA

 (i)

Serum deteriorates within one year. (ii) It interferes in downstream processing (Recovery) and purification of product. (iii) It may stimulate the growth of certain undesirable Cells. (iv) IT IS VERY COSTLY CONSTITUENT. (v) DEMAND of serum is more than ITS SUPPLY.

Organ culture is a development from tissues culture methods of research, the organ culture is able to accurately model functions of an organ in various states and conditions by the use of the actual in vitro organ itself. Parts of an organ or a whole organ can be cultured in vitro . The main objective is to maintain the architecture of the tissue and direct it towards normal development. In this technique, it is essential that the tissue is never disrupted or damaged. It thus requires careful handling. The media used for a growing organ culture are generally the same as those used for tissue culture. The techniques for organ culture can be classified into (i) those employing a solid medium and (ii) those employing liquid medium.

 DEFINITION:-

ORGAN CULTURE INVOLVES IN VITRO GROWTH AND CULTURE OG ORGANS OR TISSUE FRAGMENTS ON A SUITABLE NUTRIENT MEDIUM SO AS TO PRESERVE THEIR STRUCTURE AND FUNCTION

 Plasma

clot method Agar gel method Raft Methods Grid Method

PROCEDURES OF ORGAN CULTURE:1. PLASMA CLOT OR WATCH GLASS TECHNIQUE (FIG. 4.7A). 2. AGAR GEL METHOD (FIG. 4.7B). 3.GRID METHOD (FIG.4.7C).

 CAN

BE SUBJECTED TO CONTROLLED PHYSICOCHEMICAL ENVIRONMENT (pH, Temperature, Osmotic Pressure, Oxygen, Carbon Dioxide, Etc). AFTER SERIAL PASSAGES, HOMOGENEITY OF CELL TYPES CAN BE ACHIEVED. SINCE SMALLER QUANTITIES OF REAGENTS ARE REQUIRED IN VITRO SO ARE MORE ECONOMICAL. STUDIES ON PATTERNS OF GROWTH AND DIFFERENTIATION OF ORGAN RUDIMENTS.

 ANIMAL

CELLS WERE FOUND TO BE VERY FASTIDIOUS AND COMPLEX IN THEIR NUTRITIONAL REQUIREMENTS THAN THE MOCROBIAL AND PLANT CELLS. TO REGULAT AND INTERPRET THE BEHAVIOUR OF ANIMAL CELLS, MORE EXPERTISE IS REQUIRED. UNSTABLE ANEUPLOID CHROMOSOMAL COMBINATIONS MAY BE PRODUCED. ORGAN CULTURES ARE SHORT LIVED.

THANK

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