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4
P13- P20
DETERMINATION of
CHOLESTEROL and
TRIGLYCERIDE IN SERUM
TC—
(CHO-PAP Enzymatic Endpoint Method, Leadman Kit)
TG—
(GPO Enzymatic Endpoint Method, Leadman Kit)
Objective
Comprehend the principle and the
method of determination of TC and TG in
serum
Associate with the clinical significance of
TC and TG .
1. Determination of
Total Cholesterol (TC) in Serum
Chol esterase
Chol E + H2O Chol + FA
Chol oxidase
Chol + O2 Cholestene-3-one +H2O2
POD
2 H2O2 + Phenol + 4-aminoantipyrene Quinoneimine +
Red color H2O
determine A520 nm
2. Determination of
Triglycerides (TC) in Serum
Lipases
TG + H2O Glycerol + FA
Glycerol Kinase
Glycerol + ATP Glycerol-1-phosphate + ADP
GPO
Glycerol-1-phosphate +O2 Dihydroxyacetone phosphate +
H2O2
POD
2 H2O2 + 4-aminoantipyrine + 4 Chlorophenol