Mtodo

Ten healthy young male subjects (21.8 0.4

years, 1.78 0.04 m, 75.6 2.9 kg; mean SE)
engaged in unilateral resistance training for 8
week (3 days/week).

Exercises were knee extension and leg press
performed at 8090% of the subjects single
repetition maximum (1RM).
Mtodo
Blood samples were collected in the acute
period before and after the Wrst training bout
and following the last training bout and analyzed
for total testosterone, free-testosterone,
luteinizing hormone, sex hormone binding
globulin, growth hormone, cortisol, and insulin-
like growth factor-1.

Thigh muscle cross sectional area (CSA) and
muscle Wbre CSA by biopsy (vastus lateralis)
were measured pre- and post-training.
Concluso
In conclusion, unilateral training induced
local muscle hypertrophy only in the
exercised limb, which occurred in the
absence of changes in systemic hormones
that ostensibly play a role in muscle
hypertrophy.

Mtodo
In a single-blind, randomized protocol,
resistance-trained males were matched for
strength and placed into one of two groups; the
PRE-POST group consumed a supplement (1
gIkgj1 body weight) containing protein/creatine/
glucose immediately before and after RE.

The MOR-EVE group consumed the same dose
of the same supplement in the morning and late
evening.
Mtodo
All assessments were completed the week
before and after 10 wk of structured, supervised
RE training.

Assessments included strength (1RM, three
exercises), body composition (DEXA), and
vastus lateralis muscle biopsies for
determination of muscle fiber type (I, IIa, IIx),
cross-sectional area (CSA), contractile protein,
creatine (Cr), and glycogen content.

Concluso
Supplement timing represents a simple but
effective strategy that enhances the
adaptations desired from RE-training.

Muscle size and strength are
increased following walk
training with restricted venous
blood flow from the leg.

J.Appl. Physiol. 2006.
CLULAS SATLITES
Fisiologia e Biologia Molecular
Msculo Esqueltico
Exibe grande capacidade para se adaptar a
demandas fisiolgicas do crescimento,
treinamento e leso.

Esse capacidade atribuda a uma pequena
populao de clulas chamadas de Clulas
Satlites.

Em 1961, Mauro a descobriu e as denominou
de Satlites devido a sua localizao no
msculo.
Localizao das Satlites
Localizam-se na periferia muscular, num
espao compreendido entre a lamina
basal e o sarcolema.

Diferem completamente da Clula
Muscular, qual situa-se envolvida pelo
sarcolema (Membrana Plasmtica da
Fibra Muscular).
Funo das Clulas Satlites
Em condies fisiolgicas inalteradas,
permanecem em estado Quiescente, e no
se proliferam.

Em resposta a estmulos como microtrauma,
tornam-se ativas, proliferam e expressam
marcadores da Miognese (Mioblastos).

Mioblastos Regenerao Muscular
Regenerao Muscular
O processo de regenerao muscular
envolve Fatores de Crescimento e uma
seqncia de eventos celulares, quais
resultam na regulao da populao das
clulas satlites.

Dentre todos os fatores de crescimento
conhecidos, dois esto bem documentados
na literatura: o IGF-1 e o HGF
Fator de Crescimento Insulnico
IGF-1

Importante na regulao do metabolismo
da Insulina;

Presentes em processos de regenerao
muscular, proliferando e diferenciando a
populao de clulas satlites.

IGF-1, Sobrecarga Muscular e
Exerccio Excntrico

Muscular
[IGF-1] Hipertrofia
e/ou Hiperplasia.


Vias de Sinalizao do IGF-1
MAP (Protena Mitognica Ativa) e PI-3K
(FosfatidilInositol-3-OH Kinase) so vias
que tem sido ativadas na proliferao de
Clulas Satlites.

A Diferenciao parece ser ativada pela
via do PI-3K.
Processo do Reparo Celular

O aumento da Massa muscular via
treinamento resistido ocorre via Ativao,
Proliferao, Migrao e Fuso para
fibras musculares existentes.

Migrao das Clulas satlites
O processo de Migrao das Clulas
Satlites depende da integridade da
Lamina Basal.

Aps a ruptura da Lamina Basal, as Clulas
Satlites usam tecidos adjacentes como
ponte para migrarem ao local da Leso.

Em resposta ao trauma sem ruptura de
membrana, as Clulas Satlites migram
sob a Lamina basal da poro intacta da
clula para a parte lesada.

Migrao das Clulas Satlites
Regenerao
Toigo & Boutellier, 2006.
Domnio Mio-Nuclear
Como as CLULAS SATLITES
funcionam ?
[Mioncleos]

Trasncrio
Kadi, 2000