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I’m
Staphylococcus
aureus
And I’m
Streptococcus
agalactiae
Nutrient Requirements
Energy Source
Phototroph
Uses light as an energy source
Chemotroph
Uses energy from the oxidation of
reduced chemical compounds
Electron (Reduction potential)
Source
Organotroph
Uses reduced organic compounds as a
source for reduction potential
Lithotroph
Uses reduced inorganic compounds as a
source for reduction potential
Carbon source
Autotroph
Can use CO2 as a sole carbon source
(Carbon fixation)
Heterotroph
Requires an organic carbon source;
cannot use CO2 as a carbon source
Nitrogen source
Organic nitrogen
Primarily from the catabolism of amino acids
Oxidized forms of inorganic nitrogen
Nitrate (NO32-) and nitrite (NO2-)
Reduced inorganic nitrogen
Ammonium (NH4+)
Dissolved nitrogen gas (N2) (Nitrogen
fixation)
Phosphate source
Organic phosphate
Inorganic phosphate (H2PO4- and HPO42-)
Sulfur source
Organic sulfur
Oxidized inorganic sulfur
Sulfate (SO42-)
Reduced inorganic sulfur
Sulfide (S2- or H2S)
Elemental sulfur (So)
Special requirements
Amino acids
Nucleotide bases
Enzymatic cofactors or “vitamins”
What are the factors that
Influenced the growth of
bacteria?? Hmmm…
I’m
streptococcus
pyogenes
Oxygen requirements
Aerobes
Obligate
facultative
Anaerobe
Obligate
facultative
microaerophillic
Thermal requirement
Cryophillic or Psychrophiles
Grows well at 0ºC; optimally between
0ºC – 15ºC
Mesophillic
Optimum around 20 – 45ºC
Thermophillic
Optimum around 55 – 65 ºC
Thermoduric
Optimum around 80 – 113 ºC
pH requirement
Acidophillic
Grow optimally between ~pH 0 and 5.5
Basophillic/alkalophiles
Grow optimally between pH 8 – 11.5
Neutrophiles
Grow optimally between pH 5.5 and 8
Salt concentration
Phosphate group
Pentose sugar
Nitrogenous base
Structure of DNA
Double stranded (double helix)
Chains of nucleotides
5’ to 3’ (strands are anti-parallel)
Complimentary base pairing
A-T
G-C
DNA Structure
Phosphate-P
Sugar-blue
Bases-ATGC
DNA Replication
Bacteria have closed, circular DNA
Genome: genetic material in an
organism
E. coli
4 million base pairs
1 mm long (over 1000 times larger that
actual bacterial cell)
DNA takes up around 10% of cell volume
Protein Synthesis
DNA------- mRNA------
protein
transcription translation
Central Dogma
of Molecular Genetics
Transcription
One strand of DNA used as a template to
make a complimentary strand of mRNA
Promoter/RNA polymerase/termination
site/5’ to 3’
Ways in which RNA & DNA differ:
RNA is ss
RNA sugar is ribose
Base pairing-A-U
Transcription
Types of RNA
Three types:
mRNA: messenger RNA
Contains 3 bases ( codon)
rRNA: ribosomal RNA
Comprises the 70 S ribosome
tRNA: transfer RNA
Transfers amino acids to ribosomes for protein
synthesis
Contains the anticodon (3 base sequence that is
complimentary to codon on mRNA)
Genetic Code
DNA: triplet code